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The map label for this gene is trmL [H]

Identifier: 182681428

GI number: 182681428

Start: 1039132

End: 1039599

Strand: Reverse

Name: trmL [H]

Synonym: XfasM23_0879

Alternate gene names: 182681428

Gene position: 1039599-1039132 (Counterclockwise)

Preceding gene: 182681435

Following gene: 182681427

Centisome position: 41.0

GC content: 54.91

Gene sequence:

>468_bases
ATGTCCATGCCTCTCTACGATGTGCTGCTGTACCAGCCCGAAATACCCCCCAACACCGGCAATGTCATCCGCCTGTGTGC
CAATACTGGCGCGCGGCTACATTTGATCGAACCACTCGGTTTTGATCTGGATGACCGGCAACTCAAGCGTGCTGGATTGG
ACTATCACGAATACGCCCGGATGCAGGTACATCCCAACCTGAGCAGTGCACTGGCTGCAATCGCACCACGACGCCTATTT
GCGCTCAGTACACGTGGCCAAGTGCGCTATGACACCCCGACTTTCCGCACGGGAGATGCTTTTATTTTCGGTCCGGAAAC
CCGCGGTTTGCCAGACACTGTGTTGCATACCATCCCGGAGTCTCAACGCCTGTATTTACCGATGCAACCACAAAGCCGCA
GCTTAAACCTCTCCAATGCCGTCGCGGTGATGGTATTTGAGGCATGGCGGCAACAGGGTTTTCAATGA

Upstream 100 bases:

>100_bases
CCTTCAGAGCGTCACCATCATTGATGCGTTACTCTGCTCATTAAGATGCGCCGAACAAATTAAACAGTGGCTGCCTCGAC
GCTGACGCTACACTGATCTC

Downstream 100 bases:

>100_bases
CAATGACAATGACAATGACAATGACAATGACAATGACAATATTGTGCTGATCTTGCTCATGAGCAACACTACGTTAAGCC
AGTGAAGCTAAGAGCGTGCT

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MSMPLYDVLLYQPEIPPNTGNVIRLCANTGARLHLIEPLGFDLDDRQLKRAGLDYHEYARMQVHPNLSSALAAIAPRRLF
ALSTRGQVRYDTPTFRTGDAFIFGPETRGLPDTVLHTIPESQRLYLPMQPQSRSLNLSNAVAVMVFEAWRQQGFQ

Sequences:

>Translated_155_residues
MSMPLYDVLLYQPEIPPNTGNVIRLCANTGARLHLIEPLGFDLDDRQLKRAGLDYHEYARMQVHPNLSSALAAIAPRRLF
ALSTRGQVRYDTPTFRTGDAFIFGPETRGLPDTVLHTIPESQRLYLPMQPQSRSLNLSNAVAVMVFEAWRQQGFQ
>Mature_154_residues
SMPLYDVLLYQPEIPPNTGNVIRLCANTGARLHLIEPLGFDLDDRQLKRAGLDYHEYARMQVHPNLSSALAAIAPRRLFA
LSTRGQVRYDTPTFRTGDAFIFGPETRGLPDTVLHTIPESQRLYLPMQPQSRSLNLSNAVAVMVFEAWRQQGFQ

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=150, Percent_Identity=55.3333333333333, Blast_Score=189, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17539; Mature: 17408

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00104 EPSP_SYNTHASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMPLYDVLLYQPEIPPNTGNVIRLCANTGARLHLIEPLGFDLDDRQLKRAGLDYHEYAR
CCCCCEEEEEECCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHCCCHHHEEE
MQVHPNLSSALAAIAPRRLFALSTRGQVRYDTPTFRTGDAFIFGPETRGLPDTVLHTIPE
EEECCCHHHHHHHHCCHHEEEECCCCEEEECCCCEECCCEEEECCCCCCCCHHHHHHCCC
SQRLYLPMQPQSRSLNLSNAVAVMVFEAWRQQGFQ
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SMPLYDVLLYQPEIPPNTGNVIRLCANTGARLHLIEPLGFDLDDRQLKRAGLDYHEYAR
CCCCEEEEEECCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHCCCHHHEEE
MQVHPNLSSALAAIAPRRLFALSTRGQVRYDTPTFRTGDAFIFGPETRGLPDTVLHTIPE
EEECCCHHHHHHHHCCHHEEEECCCCEEEECCCCEECCCEEEECCCCCCCCHHHHHHCCC
SQRLYLPMQPQSRSLNLSNAVAVMVFEAWRQQGFQ
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA