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The map label for this gene is rpoN [H]

Identifier: 182681225

GI number: 182681225

Start: 795037

End: 796377

Strand: Reverse

Name: rpoN [H]

Synonym: XfasM23_0670

Alternate gene names: 182681225

Gene position: 796377-795037 (Counterclockwise)

Preceding gene: 182681226

Following gene: 182681224

Centisome position: 31.41

GC content: 53.47

Gene sequence:

>1341_bases
ATGACACCCCAGCTACACCAAAGCATCCGTCTGCTGCAAATGTCCAACGCCGAATTGCAACTGGAAATCACCGCCGCGAT
AGAGAGCAATCCACTCTTGGACTGGGCCGAGAACCAATCATCGGAGACCAGCGAAGCTACCGAAAGGGAAGTAGAAACAG
AAGATTGGCAAGCCAAAGAATCCGCCTGGGATATCAACGGCAGTAAAAGCAACGAAAATGACGACAACAACCCTGCTGAA
CGTCTTACCACCGGGAACACTCTCACTGATCACTTGCTGTGGCAACTGCACCTCTCCCCACTGTCAGCACGTGATTACCA
AATAGGCACCGTCTTGATTGATGCCATAGAAGAAGATGGTTACCTGCGTGAACTTTTGTCCACACTGACTGCAACCCTGG
CCCCGTTATCCGTAGATGAAAGCGAACTGCTACCAGTATTACGACACATTCAATCTTTTGACCCGAGCGGCGTGGCTGCA
CGCTCGCTGGCCGAGTGCTTGACACTGCAACTGAAATCATTGAGAGAAGCGATACCCGGACGCACACTTGCACTGCAGAT
TGTCAACAGCCCACTGCTCGAACAATTGCCGCGCAGTGGTGTCACCGGACTGGCACGCGAACTGAAACGACCCTCCCAAG
ACGTCCAACAGGCAGTGGAATTGATCCGTGGACTGGACCCTCGCCCAGGTAAGAAAATTGGCGACTTAGAAGTTGGAACC
TACGTTGTACCGGACTGTGTGATCTGGCGCCAACATGGGTTGTGGCAAGCCGCGTTGTTGGAACGTGCATTCCCACAGGT
CGTCCTCCACCGCGGCTACGAACAGCTCATCAACAGCTGCAATGACGCCAATGCGAGCTACCTACGCAACCAACTCCAGG
AAGCACGGTGGCTGCTCAAGGGCCTGGAAGCACGCGGTGAAACCTTGTTAAAAGTGGTAAACAGCCTCATACGTCACCAA
GCGGGATTCTTGGAATTCGGCCAACACGCGTTACGTCCATTGACTATCCGCGAATTAGCAACGGAACTTGCCCTGCATGA
ATCCACCGTATCCCGTGCAATTGCTGGCAAGTATGTACGCACCCCACGTGGCACCTTACCGCTACGCACCTTTTTCGCTT
CAGGAATCAGCACCGACAGCGGCGGAGAGACCTCCAGCTCTGCCATTCAAGCAATCATCAGACGTCTCATAGAAACTGAA
AACCCCCGCAAGCCACTCTCTGACGCCAAACTAGCTGAATTACTGAAGACATCCGGTATCCCAGTAGCGCGACGCACCAT
AGCAAAGTATCGTGATGCTATGAACATCTCCGCATCCCACGAACGCGTCCGAGTCCTCTGA

Upstream 100 bases:

>100_bases
TCCACCTGTGATCGACATCTAACGCCGATGATGGTCAGTACGAATAAACGATATGACATTGAAAGCAAGACTGCGAACAT
CCATCGGACAACAGCTGGTC

Downstream 100 bases:

>100_bases
TCTATCTATTTCCGGCGTCTCAGCGCCAAACAGGTGGATCAAACAGGAATAGGATGATTGATTAACGTAATAACCAATGG
GAGGTATCCGATGCACATCG

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTPQLHQSIRLLQMSNAELQLEITAAIESNPLLDWAENQSSETSEATEREVETEDWQAKESAWDINGSKSNENDDNNPAE
RLTTGNTLTDHLLWQLHLSPLSARDYQIGTVLIDAIEEDGYLRELLSTLTATLAPLSVDESELLPVLRHIQSFDPSGVAA
RSLAECLTLQLKSLREAIPGRTLALQIVNSPLLEQLPRSGVTGLARELKRPSQDVQQAVELIRGLDPRPGKKIGDLEVGT
YVVPDCVIWRQHGLWQAALLERAFPQVVLHRGYEQLINSCNDANASYLRNQLQEARWLLKGLEARGETLLKVVNSLIRHQ
AGFLEFGQHALRPLTIRELATELALHESTVSRAIAGKYVRTPRGTLPLRTFFASGISTDSGGETSSSAIQAIIRRLIETE
NPRKPLSDAKLAELLKTSGIPVARRTIAKYRDAMNISASHERVRVL

Sequences:

>Translated_446_residues
MTPQLHQSIRLLQMSNAELQLEITAAIESNPLLDWAENQSSETSEATEREVETEDWQAKESAWDINGSKSNENDDNNPAE
RLTTGNTLTDHLLWQLHLSPLSARDYQIGTVLIDAIEEDGYLRELLSTLTATLAPLSVDESELLPVLRHIQSFDPSGVAA
RSLAECLTLQLKSLREAIPGRTLALQIVNSPLLEQLPRSGVTGLARELKRPSQDVQQAVELIRGLDPRPGKKIGDLEVGT
YVVPDCVIWRQHGLWQAALLERAFPQVVLHRGYEQLINSCNDANASYLRNQLQEARWLLKGLEARGETLLKVVNSLIRHQ
AGFLEFGQHALRPLTIRELATELALHESTVSRAIAGKYVRTPRGTLPLRTFFASGISTDSGGETSSSAIQAIIRRLIETE
NPRKPLSDAKLAELLKTSGIPVARRTIAKYRDAMNISASHERVRVL
>Mature_445_residues
TPQLHQSIRLLQMSNAELQLEITAAIESNPLLDWAENQSSETSEATEREVETEDWQAKESAWDINGSKSNENDDNNPAER
LTTGNTLTDHLLWQLHLSPLSARDYQIGTVLIDAIEEDGYLRELLSTLTATLAPLSVDESELLPVLRHIQSFDPSGVAAR
SLAECLTLQLKSLREAIPGRTLALQIVNSPLLEQLPRSGVTGLARELKRPSQDVQQAVELIRGLDPRPGKKIGDLEVGTY
VVPDCVIWRQHGLWQAALLERAFPQVVLHRGYEQLINSCNDANASYLRNQLQEARWLLKGLEARGETLLKVVNSLIRHQA
GFLEFGQHALRPLTIRELATELALHESTVSRAIAGKYVRTPRGTLPLRTFFASGISTDSGGETSSSAIQAIIRRLIETEN
PRKPLSDAKLAELLKTSGIPVARRTIAKYRDAMNISASHERVRVL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen utilization genes [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=466, Percent_Identity=39.2703862660944, Blast_Score=327, Evalue=8e-91,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 49531; Mature: 49400

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQLHQSIRLLQMSNAELQLEITAAIESNPLLDWAENQSSETSEATEREVETEDWQAKE
CCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHH
SAWDINGSKSNENDDNNPAERLTTGNTLTDHLLWQLHLSPLSARDYQIGTVLIDAIEEDG
HCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCH
YLRELLSTLTATLAPLSVDESELLPVLRHIQSFDPSGVAARSLAECLTLQLKSLREAIPG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
RTLALQIVNSPLLEQLPRSGVTGLARELKRPSQDVQQAVELIRGLDPRPGKKIGDLEVGT
CEEEHHHHCCHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
YVVPDCVIWRQHGLWQAALLERAFPQVVLHRGYEQLINSCNDANASYLRNQLQEARWLLK
EECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GLEARGETLLKVVNSLIRHQAGFLEFGQHALRPLTIRELATELALHESTVSRAIAGKYVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPRGTLPLRTFFASGISTDSGGETSSSAIQAIIRRLIETENPRKPLSDAKLAELLKTSGI
CCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
PVARRTIAKYRDAMNISASHERVRVL
CHHHHHHHHHHHHCCCCCCCCCEECC
>Mature Secondary Structure 
TPQLHQSIRLLQMSNAELQLEITAAIESNPLLDWAENQSSETSEATEREVETEDWQAKE
CCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHH
SAWDINGSKSNENDDNNPAERLTTGNTLTDHLLWQLHLSPLSARDYQIGTVLIDAIEEDG
HCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCH
YLRELLSTLTATLAPLSVDESELLPVLRHIQSFDPSGVAARSLAECLTLQLKSLREAIPG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
RTLALQIVNSPLLEQLPRSGVTGLARELKRPSQDVQQAVELIRGLDPRPGKKIGDLEVGT
CEEEHHHHCCHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
YVVPDCVIWRQHGLWQAALLERAFPQVVLHRGYEQLINSCNDANASYLRNQLQEARWLLK
EECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
GLEARGETLLKVVNSLIRHQAGFLEFGQHALRPLTIRELATELALHESTVSRAIAGKYVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPRGTLPLRTFFASGISTDSGGETSSSAIQAIIRRLIETENPRKPLSDAKLAELLKTSGI
CCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
PVARRTIAKYRDAMNISASHERVRVL
CHHHHHHHHHHHHCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969534 [H]