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The map label for this gene is mrdA [H]

Identifier: 182681148

GI number: 182681148

Start: 696899

End: 698962

Strand: Direct

Name: mrdA [H]

Synonym: XfasM23_0590

Alternate gene names: 182681148

Gene position: 696899-698962 (Clockwise)

Preceding gene: 182681147

Following gene: 182681149

Centisome position: 27.48

GC content: 54.12

Gene sequence:

>2064_bases
ATGAGCGTGCATCATCCACAAGCAAAGCATACGTATGCCGAAGTTGAGCAGTTTCGCCGCCGTGCTTTGCTGAGCTTGTT
TGTTGTAGTGGTGTGTCTGTTTGGTTTGGTTGGCTGGTACTTCAAGTTGCAGGTGCTTGATCATGAGATCTATGCGACTC
GTTCTGACGCCAATCGGATCAAATTGCATCCAGTCGTGCCAGGGCGCGGAATGATATATGACCGGGACGGACGCCTTTTG
GCGGATAACGTGCCTGCATTCCGATTGGACGTGATGCCGGATAAGGTTGCCGACATGGAAGCAATGCTTGTTGAACTGGG
AAAGGTGATCCCGCTTTCAGAGGATGAACTGGAGCGTTTTCACCGTGAGCGTAAGGTGCGTCGAAGATTTATGCCGGTGA
CATTGAAGCTGCGCATGAGTGAGGAGGAGATGGCGCGTTTTGCAGTTGATCGGTGGCGTTTTCCTGGTGTGGAGTTGGAA
CCCTATTTCACTCGCCGTTATCTTTATAGCGACTTGTTTGCTCACGTGATCGGCTATGTCGGTCGTATCGACGAAAAGGA
CTTGGAGACGCTGAACGGTGGGGATGCTGCATTGACCTACATCGGTAAATCGGGCCTGGAGCGCTACTACGAGCAGGTGT
TGCGTGGCAAGATCGGTTATCAGCAAATCGAAACAAATGTGCAAGGACGTGCGATTCGCAGTCTGGGACGGGTAACGGCA
CAAGCGGGTGCCGACTTGCGGTTGTCGATTGACGCTGACTTGCAGCGTGCGATGGTCGCTGCGTTTGGTCAATATGAAGG
TGCGGCGGTGGCGATGGATCCGCGCAGCGGGGAAATTCTGGGGATGGTGAGTTTACCCAGCTACGATCCCAATTTATTTG
TTAATGGCATTTCTCATGCTCAATTCAATGCGCTTAATGCGAATCCTTCGCGACCGCAATTTAACCGTTTGGTCTCTGGC
GGCGTGGCTCCTGGTTCTACACTCAAACCCTTGATTGCGTTGGCTGGATTGGATGCCGGCGTGCGTCGTCCCGAGGACCG
GATTCTTTCCACCGGTATGTTCTACCTGCCCGGTGTCAATCGGGGTTGGGGAGATTCGCATCGTGGTGGTCATGGTTGGA
CTGATTTGCGTAAATCGATCGCACAGTCGGTTAATACTTACTACTATAAATTAGCGTTGGATCTGGGCATTGAGCGCTTC
ACCGACTACATGCGTCGCTATGGCCTCGGTCAGCTCACCGGGGTCGATTTGACCGGAGAGATTAGCGGTATTCTCCCTTC
CCTCGAATATAAGCGTAAGGTGCGTAAGCAGGCTTGGTATCAGGGAGATACGGTCAATGTTTCGATTGGGCAGGGAGACT
GGAAAGTGACCCTGCTTCAGTTAGTACGTAGCGTTGCTGGCTTGGCCAGTGGTCACATGAACCGCCCGCACTTGGTGGTG
GCCGAGCGTGCCGCCTTCAGCCAGCCGTGGCAGGTGTTGCCGCAACCGCCATCACAGCCAATTAGCTCGAACCCGGGGCA
TGTGCAGGTGGTACGTGAAGGGATGATAGATACCATGCGTCCTGGAGGGACCGGCTACCAAGCTGCGATTGGTGCACCTT
ATTCGATTGCTGGCAAAACAGGCACTGCGCAAGTTGCCAGCCGTAAGGGGGAGGCTGCCGTTGATCCGCGCAACTTACCA
ATGCATTTGCGTCATCGTGCGTTATTCGTTGGTTTTGCTCCTGCTGATAATCCGACGATTGCGGTTGCGGTTGCGGTCGA
GGGCGGCGGGTATGGTGCTAGCACTGCTGCACCCATTGCACGCAAGATTTTTGATGCTTGGTTGCTAGGCAAGTTGCCAG
AGGGCTTGGAGCCTTTGGACAGTGCGCGAGGTCGTACCGCGATTGGTTTGACTCAGTTTGATATGACTGTTGCCGGGGTA
TGCGAAGCTGGAAACCGTGCTGCCTTGCAACTGGGAGAAATACAGGTTTCGGTTGCTGGTGGAATGCTAAATTCAACCGC
CATGATGCCGGTTGCGCCACGTGCGCCAGTGACTCTCGACTCATTGCCGGAGGAAGACCCTTGA

Upstream 100 bases:

>100_bases
AGTTATTGGTCGGCACCGCTATTGGGAATGTTGTTGTGGGCACCGTTGTTTGTGTTGCTGGATGCGTTGCGACTAGGTCA
TGGGAGTCGGAAGTGATCCG

Downstream 100 bases:

>100_bases
GGGATTTGCTGCGCTTGCTTGTGGATCTGGTGTTGCGCTTTACGCGCACTCTGGATTTGCCGCTGTGCTTGACTCTTGGG
GCATTGATGGTGATTGGACT

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MSVHHPQAKHTYAEVEQFRRRALLSLFVVVVCLFGLVGWYFKLQVLDHEIYATRSDANRIKLHPVVPGRGMIYDRDGRLL
ADNVPAFRLDVMPDKVADMEAMLVELGKVIPLSEDELERFHRERKVRRRFMPVTLKLRMSEEEMARFAVDRWRFPGVELE
PYFTRRYLYSDLFAHVIGYVGRIDEKDLETLNGGDAALTYIGKSGLERYYEQVLRGKIGYQQIETNVQGRAIRSLGRVTA
QAGADLRLSIDADLQRAMVAAFGQYEGAAVAMDPRSGEILGMVSLPSYDPNLFVNGISHAQFNALNANPSRPQFNRLVSG
GVAPGSTLKPLIALAGLDAGVRRPEDRILSTGMFYLPGVNRGWGDSHRGGHGWTDLRKSIAQSVNTYYYKLALDLGIERF
TDYMRRYGLGQLTGVDLTGEISGILPSLEYKRKVRKQAWYQGDTVNVSIGQGDWKVTLLQLVRSVAGLASGHMNRPHLVV
AERAAFSQPWQVLPQPPSQPISSNPGHVQVVREGMIDTMRPGGTGYQAAIGAPYSIAGKTGTAQVASRKGEAAVDPRNLP
MHLRHRALFVGFAPADNPTIAVAVAVEGGGYGASTAAPIARKIFDAWLLGKLPEGLEPLDSARGRTAIGLTQFDMTVAGV
CEAGNRAALQLGEIQVSVAGGMLNSTAMMPVAPRAPVTLDSLPEEDP

Sequences:

>Translated_687_residues
MSVHHPQAKHTYAEVEQFRRRALLSLFVVVVCLFGLVGWYFKLQVLDHEIYATRSDANRIKLHPVVPGRGMIYDRDGRLL
ADNVPAFRLDVMPDKVADMEAMLVELGKVIPLSEDELERFHRERKVRRRFMPVTLKLRMSEEEMARFAVDRWRFPGVELE
PYFTRRYLYSDLFAHVIGYVGRIDEKDLETLNGGDAALTYIGKSGLERYYEQVLRGKIGYQQIETNVQGRAIRSLGRVTA
QAGADLRLSIDADLQRAMVAAFGQYEGAAVAMDPRSGEILGMVSLPSYDPNLFVNGISHAQFNALNANPSRPQFNRLVSG
GVAPGSTLKPLIALAGLDAGVRRPEDRILSTGMFYLPGVNRGWGDSHRGGHGWTDLRKSIAQSVNTYYYKLALDLGIERF
TDYMRRYGLGQLTGVDLTGEISGILPSLEYKRKVRKQAWYQGDTVNVSIGQGDWKVTLLQLVRSVAGLASGHMNRPHLVV
AERAAFSQPWQVLPQPPSQPISSNPGHVQVVREGMIDTMRPGGTGYQAAIGAPYSIAGKTGTAQVASRKGEAAVDPRNLP
MHLRHRALFVGFAPADNPTIAVAVAVEGGGYGASTAAPIARKIFDAWLLGKLPEGLEPLDSARGRTAIGLTQFDMTVAGV
CEAGNRAALQLGEIQVSVAGGMLNSTAMMPVAPRAPVTLDSLPEEDP
>Mature_686_residues
SVHHPQAKHTYAEVEQFRRRALLSLFVVVVCLFGLVGWYFKLQVLDHEIYATRSDANRIKLHPVVPGRGMIYDRDGRLLA
DNVPAFRLDVMPDKVADMEAMLVELGKVIPLSEDELERFHRERKVRRRFMPVTLKLRMSEEEMARFAVDRWRFPGVELEP
YFTRRYLYSDLFAHVIGYVGRIDEKDLETLNGGDAALTYIGKSGLERYYEQVLRGKIGYQQIETNVQGRAIRSLGRVTAQ
AGADLRLSIDADLQRAMVAAFGQYEGAAVAMDPRSGEILGMVSLPSYDPNLFVNGISHAQFNALNANPSRPQFNRLVSGG
VAPGSTLKPLIALAGLDAGVRRPEDRILSTGMFYLPGVNRGWGDSHRGGHGWTDLRKSIAQSVNTYYYKLALDLGIERFT
DYMRRYGLGQLTGVDLTGEISGILPSLEYKRKVRKQAWYQGDTVNVSIGQGDWKVTLLQLVRSVAGLASGHMNRPHLVVA
ERAAFSQPWQVLPQPPSQPISSNPGHVQVVREGMIDTMRPGGTGYQAAIGAPYSIAGKTGTAQVASRKGEAAVDPRNLPM
HLRHRALFVGFAPADNPTIAVAVAVEGGGYGASTAAPIARKIFDAWLLGKLPEGLEPLDSARGRTAIGLTQFDMTVAGVC
EAGNRAALQLGEIQVSVAGGMLNSTAMMPVAPRAPVTLDSLPEEDP

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=606, Percent_Identity=39.6039603960396, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1786272, Length=616, Percent_Identity=28.4090909090909, Blast_Score=171, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 75387; Mature: 75256

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVHHPQAKHTYAEVEQFRRRALLSLFVVVVCLFGLVGWYFKLQVLDHEIYATRSDANRI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCCEEECCCCCEE
KLHPVVPGRGMIYDRDGRLLADNVPAFRLDVMPDKVADMEAMLVELGKVIPLSEDELERF
EEEEECCCCCEEEECCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
HRERKVRRRFMPVTLKLRMSEEEMARFAVDRWRFPGVELEPYFTRRYLYSDLFAHVIGYV
HHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GRIDEKDLETLNGGDAALTYIGKSGLERYYEQVLRGKIGYQQIETNVQGRAIRSLGRVTA
CCCCHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHH
QAGADLRLSIDADLQRAMVAAFGQYEGAAVAMDPRSGEILGMVSLPSYDPNLFVNGISHA
HCCCCEEEEECHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCCCCEEEECCCCC
QFNALNANPSRPQFNRLVSGGVAPGSTLKPLIALAGLDAGVRRPEDRILSTGMFYLPGVN
EEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCEEEECCCC
RGWGDSHRGGHGWTDLRKSIAQSVNTYYYKLALDLGIERFTDYMRRYGLGQLTGVDLTGE
CCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEHHHHCHHHHHHHHHHCCCCCEECCCCCCC
ISGILPSLEYKRKVRKQAWYQGDTVNVSIGQGDWKVTLLQLVRSVAGLASGHMNRPHLVV
HHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
AERAAFSQPWQVLPQPPSQPISSNPGHVQVVREGMIDTMRPGGTGYQAAIGAPYSIAGKT
ECCHHCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCEEECCCCEEECCCC
GTAQVASRKGEAAVDPRNLPMHLRHRALFVGFAPADNPTIAVAVAVEGGGYGASTAAPIA
CCHHHHHCCCCCCCCCCCCCHHEECEEEEEEECCCCCCEEEEEEEEECCCCCCCHHHHHH
RKIFDAWLLGKLPEGLEPLDSARGRTAIGLTQFDMTVAGVCEAGNRAALQLGEIQVSVAG
HHHHHHHHHHCCCCCCCCHHHCCCCEEEEEEHHHHHHHHHHCCCCCCEEEEEEEEEEEEC
GMLNSTAMMPVAPRAPVTLDSLPEEDP
CEECCCCCCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure 
SVHHPQAKHTYAEVEQFRRRALLSLFVVVVCLFGLVGWYFKLQVLDHEIYATRSDANRI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCCEEECCCCCEE
KLHPVVPGRGMIYDRDGRLLADNVPAFRLDVMPDKVADMEAMLVELGKVIPLSEDELERF
EEEEECCCCCEEEECCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHH
HRERKVRRRFMPVTLKLRMSEEEMARFAVDRWRFPGVELEPYFTRRYLYSDLFAHVIGYV
HHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GRIDEKDLETLNGGDAALTYIGKSGLERYYEQVLRGKIGYQQIETNVQGRAIRSLGRVTA
CCCCHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHH
QAGADLRLSIDADLQRAMVAAFGQYEGAAVAMDPRSGEILGMVSLPSYDPNLFVNGISHA
HCCCCEEEEECHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCCCCCCEEEECCCCC
QFNALNANPSRPQFNRLVSGGVAPGSTLKPLIALAGLDAGVRRPEDRILSTGMFYLPGVN
EEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCEEEECCCC
RGWGDSHRGGHGWTDLRKSIAQSVNTYYYKLALDLGIERFTDYMRRYGLGQLTGVDLTGE
CCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEHHHHCHHHHHHHHHHCCCCCEECCCCCCC
ISGILPSLEYKRKVRKQAWYQGDTVNVSIGQGDWKVTLLQLVRSVAGLASGHMNRPHLVV
HHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
AERAAFSQPWQVLPQPPSQPISSNPGHVQVVREGMIDTMRPGGTGYQAAIGAPYSIAGKT
ECCHHCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCEEECCCCEEECCCC
GTAQVASRKGEAAVDPRNLPMHLRHRALFVGFAPADNPTIAVAVAVEGGGYGASTAAPIA
CCHHHHHCCCCCCCCCCCCCHHEECEEEEEEECCCCCCEEEEEEEEECCCCCCCHHHHHH
RKIFDAWLLGKLPEGLEPLDSARGRTAIGLTQFDMTVAGVCEAGNRAALQLGEIQVSVAG
HHHHHHHHHHCCCCCCCCHHHCCCCEEEEEEHHHHHHHHHHCCCCCCEEEEEEEEEEEEC
GMLNSTAMMPVAPRAPVTLDSLPEEDP
CEECCCCCCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]