Click here to switch to the map view.

The map label for this gene is parE [H]

Identifier: 182681122

GI number: 182681122

Start: 672123

End: 674012

Strand: Reverse

Name: parE [H]

Synonym: XfasM23_0564

Alternate gene names: 182681122

Gene position: 674012-672123 (Counterclockwise)

Preceding gene: 182681130

Following gene: 182681121

Centisome position: 26.58

GC content: 52.38

Gene sequence:

>1890_bases
ATGAACTCCCGCTATAACGCCGCAGACATCGAAGTTCTCACTGGCCTGGACCCAGTCAAACGCCGCCCCGGGATGTATAC
CGATACGACACGTCCCAACCATCTGGCACAGGAAGTTATTGACAATTCAGTAGACGAGGCACTTGCAGGCTACGCGAATC
GAATTGAAGTCACACTCTACAAAGACGGTAGCTGCGAAGTCGCCGACAACGGTCGTGGCATGCCAGTGGATATTCACCCA
GAAGAGAAGATTCCCGGCGTTGAATTGATCCTGACCAGGCTACACGCTGGCGGCAAATTTAGTAATCGCAACTACACCTT
TTCTGGAGGATTGCACGGCGTAGGCGTGAGCGTAGTCAACGCACTGTCCAGTAAAGTTGATCTCTTCATCAAACGCGAAG
GTAGCGAACACCACATGGAATTTCGCGAAGGCTTTGCCGTATCCAAATTGAAAGTACTCGGCACAGTCAGCAAAAAAAAT
ACAGGTACACGTCTGCGCTTCTGGCCTGATTCAAAATACTTCGACACCCCTAAATTCAACGTACGTGTATTGCGCCATCT
ACTGCGTGCCAAAGCGGTCCTCTGCCCCGGTTTGACAGTCGAATTACTTGAAGAGGCCACAGGAGAGATCAACACATGGC
ACTTCAAAGACGGCTTACGCGACTACCTTAAAGGCGAAATGACCGAAAGTGAACTGCTGCCTGCTGAGCTGTTCATTGGC
TGCCTGAAGAAAGATACCGAAGTAGTGGACTGGGCGGTCGCCTGGGTCCCCGAAGGAGAACTCACTCAAGAAAGCTACGT
CAACCTGATCCCTACCCCACAACATGGCACCCACGTCAATGGACTGCGCTCAGGATTAACCGACGCACTACGCGAATTCT
GTGACTTCCGCAATCTACTGCCACGCGGAATCAAACTGGCTCCGGAAGATGTCTGGGATCGTGTCACTTTTGTGCTGTCG
CTGAAAATGAGTGACCCCCAGTTCTCCGGTCAAACCAAGGAACGCTTATCTTCACGCCAAGCAGCTGGTTTCATTGAAAG
CACAGTGCATGACGCCTTCAGCCTATGGTTAAACCAGAACGTAGACATTGGCGAACACATTGCGCAGATCGCTATTGAAC
GTGCCAGCGCACGTCTGAAAACCGAAAAACAAGTCACCCGAAAGAAAATCACCCATGGCCCCGCACTCCCCGGAAAATTA
GCCGACTGCATCAGCCTGGATCTTTCACGCACAGAACTGTTCCTGGTAGAAGGCGACTCGGCCGGAGGCAGCGCCAAACA
AGCACGGGACAAAGATTTCCAAGCGATCCTGCCGTTACGCGGCAAAATCCTCAACACATGGGAAGTCGCCTCCGGCAGCG
TCCTCGCCTCCGATGAAGTGCATAACCTGGCAATCGCAATCGGCTGCGACCCTGGCAAAGACAATCTCTCAGGATTGCGC
TACGGCAAAATCGTCATCCTGGCCGACGCCGACTCCGACGGCCTACACATCGCCACCCTGCTTACCGCACTCTTCCTCAA
ACACTTCCCCGGACTCGTTGCAGCCGGTCACGTGTTCGTGGCAATGCCTCCCCTGTTCCGCGTCGACGTAGGCAAGCAAG
TGTTTTACGCACTAGACGATGAAGAAAAGACCATACTGCTGGAAAAGATACAGCGTGAAAAAATCAAGGGACAAATCAGC
GTCACCCGCTTCAAAGGCCTAGGAGAAATGAACCCGCAGCAACTACGCGAATCCACCATTCATCCGGATACACGCCGCCT
CGTACAACTGACCATGGATGACAACACCCAGACTTGCTCACTGATGGACATGCTACTAGCCAAAAAACGCGCAAGCGATC
GTAAACAATGGCTGGAAACAAAAGGTAACTTAGCGTCACTAGACATATAG

Upstream 100 bases:

>100_bases
CAGGAATAGCAAGACCTTCACTGAACGAACCTGAATGGCGTGCAAAAATGTGTAGCAAACAGCACACTTAGACGTTACCT
CACGCCACCCCAAAAGACGC

Downstream 100 bases:

>100_bases
CAGACCATCCATACGAACCATAAAAATCAGCAATTGCAACATTCACATAGCCCCCAAGATCCAACGCCAACATCAGGCAT
CAATGCACAACCTGTCGCAT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MNSRYNAADIEVLTGLDPVKRRPGMYTDTTRPNHLAQEVIDNSVDEALAGYANRIEVTLYKDGSCEVADNGRGMPVDIHP
EEKIPGVELILTRLHAGGKFSNRNYTFSGGLHGVGVSVVNALSSKVDLFIKREGSEHHMEFREGFAVSKLKVLGTVSKKN
TGTRLRFWPDSKYFDTPKFNVRVLRHLLRAKAVLCPGLTVELLEEATGEINTWHFKDGLRDYLKGEMTESELLPAELFIG
CLKKDTEVVDWAVAWVPEGELTQESYVNLIPTPQHGTHVNGLRSGLTDALREFCDFRNLLPRGIKLAPEDVWDRVTFVLS
LKMSDPQFSGQTKERLSSRQAAGFIESTVHDAFSLWLNQNVDIGEHIAQIAIERASARLKTEKQVTRKKITHGPALPGKL
ADCISLDLSRTELFLVEGDSAGGSAKQARDKDFQAILPLRGKILNTWEVASGSVLASDEVHNLAIAIGCDPGKDNLSGLR
YGKIVILADADSDGLHIATLLTALFLKHFPGLVAAGHVFVAMPPLFRVDVGKQVFYALDDEEKTILLEKIQREKIKGQIS
VTRFKGLGEMNPQQLRESTIHPDTRRLVQLTMDDNTQTCSLMDMLLAKKRASDRKQWLETKGNLASLDI

Sequences:

>Translated_629_residues
MNSRYNAADIEVLTGLDPVKRRPGMYTDTTRPNHLAQEVIDNSVDEALAGYANRIEVTLYKDGSCEVADNGRGMPVDIHP
EEKIPGVELILTRLHAGGKFSNRNYTFSGGLHGVGVSVVNALSSKVDLFIKREGSEHHMEFREGFAVSKLKVLGTVSKKN
TGTRLRFWPDSKYFDTPKFNVRVLRHLLRAKAVLCPGLTVELLEEATGEINTWHFKDGLRDYLKGEMTESELLPAELFIG
CLKKDTEVVDWAVAWVPEGELTQESYVNLIPTPQHGTHVNGLRSGLTDALREFCDFRNLLPRGIKLAPEDVWDRVTFVLS
LKMSDPQFSGQTKERLSSRQAAGFIESTVHDAFSLWLNQNVDIGEHIAQIAIERASARLKTEKQVTRKKITHGPALPGKL
ADCISLDLSRTELFLVEGDSAGGSAKQARDKDFQAILPLRGKILNTWEVASGSVLASDEVHNLAIAIGCDPGKDNLSGLR
YGKIVILADADSDGLHIATLLTALFLKHFPGLVAAGHVFVAMPPLFRVDVGKQVFYALDDEEKTILLEKIQREKIKGQIS
VTRFKGLGEMNPQQLRESTIHPDTRRLVQLTMDDNTQTCSLMDMLLAKKRASDRKQWLETKGNLASLDI
>Mature_629_residues
MNSRYNAADIEVLTGLDPVKRRPGMYTDTTRPNHLAQEVIDNSVDEALAGYANRIEVTLYKDGSCEVADNGRGMPVDIHP
EEKIPGVELILTRLHAGGKFSNRNYTFSGGLHGVGVSVVNALSSKVDLFIKREGSEHHMEFREGFAVSKLKVLGTVSKKN
TGTRLRFWPDSKYFDTPKFNVRVLRHLLRAKAVLCPGLTVELLEEATGEINTWHFKDGLRDYLKGEMTESELLPAELFIG
CLKKDTEVVDWAVAWVPEGELTQESYVNLIPTPQHGTHVNGLRSGLTDALREFCDFRNLLPRGIKLAPEDVWDRVTFVLS
LKMSDPQFSGQTKERLSSRQAAGFIESTVHDAFSLWLNQNVDIGEHIAQIAIERASARLKTEKQVTRKKITHGPALPGKL
ADCISLDLSRTELFLVEGDSAGGSAKQARDKDFQAILPLRGKILNTWEVASGSVLASDEVHNLAIAIGCDPGKDNLSGLR
YGKIVILADADSDGLHIATLLTALFLKHFPGLVAAGHVFVAMPPLFRVDVGKQVFYALDDEEKTILLEKIQREKIKGQIS
VTRFKGLGEMNPQQLRESTIHPDTRRLVQLTMDDNTQTCSLMDMLLAKKRASDRKQWLETKGNLASLDI

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=578, Percent_Identity=25.4325259515571, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI19913406, Length=573, Percent_Identity=24.956369982548, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1789408, Length=632, Percent_Identity=63.9240506329114, Blast_Score=857, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=552, Percent_Identity=39.8550724637681, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17535065, Length=627, Percent_Identity=24.8803827751196, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=598, Percent_Identity=23.5785953177258, Blast_Score=118, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6324241, Length=633, Percent_Identity=24.4865718799368, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI17136538, Length=624, Percent_Identity=24.8397435897436, Blast_Score=126, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 69825; Mature: 69825

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSRYNAADIEVLTGLDPVKRRPGMYTDTTRPNHLAQEVIDNSVDEALAGYANRIEVTLY
CCCCCCCCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHCHHHHHHHCCCCEEEEEEE
KDGSCEVADNGRGMPVDIHPEEKIPGVELILTRLHAGGKFSNRNYTFSGGLHGVGVSVVN
ECCCEEECCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHH
ALSSKVDLFIKREGSEHHMEFREGFAVSKLKVLGTVSKKNTGTRLRFWPDSKYFDTPKFN
HHHHHEEEEEEECCCHHHHHHHCCCHHHHEEEEEECCCCCCCCEEEECCCCCCCCCCCCH
VRVLRHLLRAKAVLCPGLTVELLEEATGEINTWHFKDGLRDYLKGEMTESELLPAELFIG
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCHHHCCCHHHHHH
CLKKDTEVVDWAVAWVPEGELTQESYVNLIPTPQHGTHVNGLRSGLTDALREFCDFRNLL
HHCCCCCEEEEEEEECCCCCCCHHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
PRGIKLAPEDVWDRVTFVLSLKMSDPQFSGQTKERLSSRQAAGFIESTVHDAFSLWLNQN
CCCCCCCCHHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VDIGEHIAQIAIERASARLKTEKQVTRKKITHGPALPGKLADCISLDLSRTELFLVEGDS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEEEECCC
AGGSAKQARDKDFQAILPLRGKILNTWEVASGSVLASDEVHNLAIAIGCDPGKDNLSGLR
CCCCCHHHCCCCCCEECCCCCCEEEEEECCCCCEEECCCCCEEEEEEECCCCCCCCCCEE
YGKIVILADADSDGLHIATLLTALFLKHFPGLVAAGHVFVAMPPLFRVDVGKQVFYALDD
ECEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHCEEEEECCCCEEEECCCEEEEEECC
EEKTILLEKIQREKIKGQISVTRFKGLGEMNPQQLRESTIHPDTRRLVQLTMDDNTQTCS
CCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHHH
LMDMLLAKKRASDRKQWLETKGNLASLDI
HHHHHHHHHHHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure
MNSRYNAADIEVLTGLDPVKRRPGMYTDTTRPNHLAQEVIDNSVDEALAGYANRIEVTLY
CCCCCCCCCEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHCHHHHHHHCCCCEEEEEEE
KDGSCEVADNGRGMPVDIHPEEKIPGVELILTRLHAGGKFSNRNYTFSGGLHGVGVSVVN
ECCCEEECCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHH
ALSSKVDLFIKREGSEHHMEFREGFAVSKLKVLGTVSKKNTGTRLRFWPDSKYFDTPKFN
HHHHHEEEEEEECCCHHHHHHHCCCHHHHEEEEEECCCCCCCCEEEECCCCCCCCCCCCH
VRVLRHLLRAKAVLCPGLTVELLEEATGEINTWHFKDGLRDYLKGEMTESELLPAELFIG
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCHHHCCCHHHHHH
CLKKDTEVVDWAVAWVPEGELTQESYVNLIPTPQHGTHVNGLRSGLTDALREFCDFRNLL
HHCCCCCEEEEEEEECCCCCCCHHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
PRGIKLAPEDVWDRVTFVLSLKMSDPQFSGQTKERLSSRQAAGFIESTVHDAFSLWLNQN
CCCCCCCCHHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VDIGEHIAQIAIERASARLKTEKQVTRKKITHGPALPGKLADCISLDLSRTELFLVEGDS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEEEECCC
AGGSAKQARDKDFQAILPLRGKILNTWEVASGSVLASDEVHNLAIAIGCDPGKDNLSGLR
CCCCCHHHCCCCCCEECCCCCCEEEEEECCCCCEEECCCCCEEEEEEECCCCCCCCCCEE
YGKIVILADADSDGLHIATLLTALFLKHFPGLVAAGHVFVAMPPLFRVDVGKQVFYALDD
ECEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHCEEEEECCCCEEEECCCEEEEEECC
EEKTILLEKIQREKIKGQISVTRFKGLGEMNPQQLRESTIHPDTRRLVQLTMDDNTQTCS
CCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHHH
LMDMLLAKKRASDRKQWLETKGNLASLDI
HHHHHHHHHHHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]