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The map label for this gene is ppsA [H]

Identifier: 182681106

GI number: 182681106

Start: 649935

End: 652334

Strand: Reverse

Name: ppsA [H]

Synonym: XfasM23_0548

Alternate gene names: 182681106

Gene position: 652334-649935 (Counterclockwise)

Preceding gene: 182681110

Following gene: 182681105

Centisome position: 25.73

GC content: 53.67

Gene sequence:

>2400_bases
ATGGCCCTAAACGGAGCATCGTACTTGAACGAAAATATCCTTTGGTTACATGAACTGCGCTTGACCGATCTGGCCCGCGT
CGGTGGTAAAAATTCCTCACTCGGCGAAATGATCGGCAATCTCACTGCCCTTGGAGTCTCTGTTCCAGGTGGCTATGCCA
CCACCGCAGCAGCATTTAAAGCCTTTATCGCTTACAACAACCTGTCCAAACGGATCTACGACAAACTAGCCACCCTTGAT
GTTGAGGACGTCAAAGCACTGACAGCTGCCGGCAAAGAAATCCGCGACTGGGTCATTGACGCTCCATTACAACCAGAACT
GGAGAAGGATATTCGTGACGCCTACGACACGTTGTCAAAAGACAATAGCGGCAACGACGTCGCCGTAGCGGTGCGCTCTT
CAGCGACCGCGGAAGATTTACCAGACGCCTCATTCGCCGGCCAACAGGAAACCTTTCTTAATGTGATCGGCGCCGACCAG
GTCGTACACAAAATTAAAGAAGTATTCGCAAGTCTTTATAACGATCGTGCAATTGCCTACCGCGTCCATCATGGCTTCAA
GCACGAGGACGTATTCCTCTCCGCCGGAATACAACTGATGGTTCGCTCGGATATCAGTGCCTCCGGCGTGCTGTTCACTC
TAGACACCGAGTCAGGCTTCCGCGAGGTGGTGTTCGTCACCGCCAGCTTCGGCCTTGGTGAAATGGTCGTACAGGGCGCC
GTCAACCCAGACGAATTCTATGTCTATAAAAAGACGCTCAAAGCTGGCAAACCTGCCATCCTGCGCCGCACACTCGGCAC
CAAACTGGTGCGCATGGTCTATTCAGACGCCCCAGGAGAGCGCGTCAAGATCGAAGATACCCCAACTGAATTACGTCACT
GCTTCGCCATCAGCGACGCTGACGTACAAGAGCTGGCGAGACAAGCACTGATTATTGAGCAGCACTACGGCCGCCCGATG
GACATTGAATGGGCTAAGGATGGGATCAGCGGCAAATTATTTATTGTGCAGGCACGTCCGGAGACAGTGAAATCACGCAG
TCACACCACCCAGATTGAACGCTTTGCACTCCAACAACCCGGCCAGATACTGACAGAGGGACGTGCCATCGGCCAGAAGA
TCGGCAGCGGCACAGCCCGTGTGGTGAACTCACTTGAGGACATGCACCACATGCAACCCGGCGATGTCCTGGTTGCAGAC
ATGACCGACCCAGACTGGGAGCCAGTCATGAAACGCGCCAGCGCGATTGTCACCAATCGCGGCGGACGTACCTGCCACGC
CGCCATTATCGCCCGTGAGCTGGGAGTACCCGCAGTGGTTGGCACCGGCAATGCGCTATCAATCATTCAAGACGGCCAAC
CCATCACGGTAAGCTGCGCCGAAGGTGACACCGGCTATATCTACGCCGGGAAACTACCGTTCGAACGCACCACAACAGAC
CTCGGCCACATGCCACCAGCGCCGCTAAAAATCATGATGAATGTTGCCAATCCGGAACGTGCGTTCGACTTTGGGCAACT
CCCGAATGCCGGTATCGGCTTGGCACGCCTGGAAATGATCATCGCCGCACACATCGGCGTGCACCCAAAAGCACTTCTGG
AATACCACAAACAAGATGCTGACACGAAACAGAAGATTGACGCCAAGATTGCCGGTTATCCTGATCCAGTCAGCTTCTAC
GTCAACCGCCTCGCCGAAGGTATTGCGACCCTGACCGCCTCAGTCGCACCCCATACGGTCATCGTGCGCCTGTCGGACTT
CAAATCCAACGAATACGCACACCTGATCGGTGGCCAGCACTATGAGCCACAAGAAGAAAACCCGATGCTCGGCTTCCGCG
GTGCCAGCCGTTACGTCGACCCTTCATTCAGCGATGCCTTCGCATTAGAGTGCAAAGCAGTACTAAAAGTCCGTAACGAG
ATGGGTCTAGATAACCTCTGGGTGATGATTCCATTTGTACGCACTTTGGAAGAAGGTCGCAAAGTGATCGACGTACTGCG
ACAAAATGGCTTAAAACAGGGAGAAAATGGTTTAAAAGTGATCATGATGTGCGAAGTGCCCTCCAACGCACTGCTGGCTG
ATGAATTCCTGGATATCTTCGACGGCTTCTCAATCGGCTCCAACGATCTCACCCAACTTTCCCTAGGTCTGGACCGCGAC
TCCTCCATCGTCGCCCCTCTATTTGACGAGCGCAATCCAGCCGTCAAAAAACTACTGTCAATGGCAATAAAAGCGGCACG
TTCCAAGGGAAAATACATTGGAATCTGCGGCCAAGGCCCATCCGATCATCCAGACTTGGCCGAATGGCTGATGCAAGAAG
GCATAGAATCCCTGTCACTGAACCCAGATACCGTCGTCGATACTTGGCTACGCTTGGCAAAACTAAAAACGGTGCACTAA

Upstream 100 bases:

>100_bases
CCGAGACATCTCACCAATTTAAGCTTGTGCAGTCTTGCCTATGCACTGCATCATACGACCACTTTAATACGGACGCGGCC
ATACAGCCCTCCTCCAGGCG

Downstream 100 bases:

>100_bases
ATGGACTGGACTAAATTACTATCAAACATCGTATCGCCTGCAACAGTGACGACGTTCCACGGCAGATTCAACTGGACACG
GATCGACTGGATCCACATCC

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 799; Mature: 798

Protein sequence:

>799_residues
MALNGASYLNENILWLHELRLTDLARVGGKNSSLGEMIGNLTALGVSVPGGYATTAAAFKAFIAYNNLSKRIYDKLATLD
VEDVKALTAAGKEIRDWVIDAPLQPELEKDIRDAYDTLSKDNSGNDVAVAVRSSATAEDLPDASFAGQQETFLNVIGADQ
VVHKIKEVFASLYNDRAIAYRVHHGFKHEDVFLSAGIQLMVRSDISASGVLFTLDTESGFREVVFVTASFGLGEMVVQGA
VNPDEFYVYKKTLKAGKPAILRRTLGTKLVRMVYSDAPGERVKIEDTPTELRHCFAISDADVQELARQALIIEQHYGRPM
DIEWAKDGISGKLFIVQARPETVKSRSHTTQIERFALQQPGQILTEGRAIGQKIGSGTARVVNSLEDMHHMQPGDVLVAD
MTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGTGNALSIIQDGQPITVSCAEGDTGYIYAGKLPFERTTTD
LGHMPPAPLKIMMNVANPERAFDFGQLPNAGIGLARLEMIIAAHIGVHPKALLEYHKQDADTKQKIDAKIAGYPDPVSFY
VNRLAEGIATLTASVAPHTVIVRLSDFKSNEYAHLIGGQHYEPQEENPMLGFRGASRYVDPSFSDAFALECKAVLKVRNE
MGLDNLWVMIPFVRTLEEGRKVIDVLRQNGLKQGENGLKVIMMCEVPSNALLADEFLDIFDGFSIGSNDLTQLSLGLDRD
SSIVAPLFDERNPAVKKLLSMAIKAARSKGKYIGICGQGPSDHPDLAEWLMQEGIESLSLNPDTVVDTWLRLAKLKTVH

Sequences:

>Translated_799_residues
MALNGASYLNENILWLHELRLTDLARVGGKNSSLGEMIGNLTALGVSVPGGYATTAAAFKAFIAYNNLSKRIYDKLATLD
VEDVKALTAAGKEIRDWVIDAPLQPELEKDIRDAYDTLSKDNSGNDVAVAVRSSATAEDLPDASFAGQQETFLNVIGADQ
VVHKIKEVFASLYNDRAIAYRVHHGFKHEDVFLSAGIQLMVRSDISASGVLFTLDTESGFREVVFVTASFGLGEMVVQGA
VNPDEFYVYKKTLKAGKPAILRRTLGTKLVRMVYSDAPGERVKIEDTPTELRHCFAISDADVQELARQALIIEQHYGRPM
DIEWAKDGISGKLFIVQARPETVKSRSHTTQIERFALQQPGQILTEGRAIGQKIGSGTARVVNSLEDMHHMQPGDVLVAD
MTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGTGNALSIIQDGQPITVSCAEGDTGYIYAGKLPFERTTTD
LGHMPPAPLKIMMNVANPERAFDFGQLPNAGIGLARLEMIIAAHIGVHPKALLEYHKQDADTKQKIDAKIAGYPDPVSFY
VNRLAEGIATLTASVAPHTVIVRLSDFKSNEYAHLIGGQHYEPQEENPMLGFRGASRYVDPSFSDAFALECKAVLKVRNE
MGLDNLWVMIPFVRTLEEGRKVIDVLRQNGLKQGENGLKVIMMCEVPSNALLADEFLDIFDGFSIGSNDLTQLSLGLDRD
SSIVAPLFDERNPAVKKLLSMAIKAARSKGKYIGICGQGPSDHPDLAEWLMQEGIESLSLNPDTVVDTWLRLAKLKTVH
>Mature_798_residues
ALNGASYLNENILWLHELRLTDLARVGGKNSSLGEMIGNLTALGVSVPGGYATTAAAFKAFIAYNNLSKRIYDKLATLDV
EDVKALTAAGKEIRDWVIDAPLQPELEKDIRDAYDTLSKDNSGNDVAVAVRSSATAEDLPDASFAGQQETFLNVIGADQV
VHKIKEVFASLYNDRAIAYRVHHGFKHEDVFLSAGIQLMVRSDISASGVLFTLDTESGFREVVFVTASFGLGEMVVQGAV
NPDEFYVYKKTLKAGKPAILRRTLGTKLVRMVYSDAPGERVKIEDTPTELRHCFAISDADVQELARQALIIEQHYGRPMD
IEWAKDGISGKLFIVQARPETVKSRSHTTQIERFALQQPGQILTEGRAIGQKIGSGTARVVNSLEDMHHMQPGDVLVADM
TDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGVPAVVGTGNALSIIQDGQPITVSCAEGDTGYIYAGKLPFERTTTDL
GHMPPAPLKIMMNVANPERAFDFGQLPNAGIGLARLEMIIAAHIGVHPKALLEYHKQDADTKQKIDAKIAGYPDPVSFYV
NRLAEGIATLTASVAPHTVIVRLSDFKSNEYAHLIGGQHYEPQEENPMLGFRGASRYVDPSFSDAFALECKAVLKVRNEM
GLDNLWVMIPFVRTLEEGRKVIDVLRQNGLKQGENGLKVIMMCEVPSNALLADEFLDIFDGFSIGSNDLTQLSLGLDRDS
SIVAPLFDERNPAVKKLLSMAIKAARSKGKYIGICGQGPSDHPDLAEWLMQEGIESLSLNPDTVVDTWLRLAKLKTVH

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=798, Percent_Identity=63.7844611528822, Blast_Score=1074, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=163, Percent_Identity=36.8098159509202, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788756, Length=396, Percent_Identity=25.7575757575758, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI48994992, Length=162, Percent_Identity=32.7160493827161, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789193, Length=403, Percent_Identity=23.5732009925558, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17564524, Length=397, Percent_Identity=25.1889168765743, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87319; Mature: 87188

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALNGASYLNENILWLHELRLTDLARVGGKNSSLGEMIGNLTALGVSVPGGYATTAAAFK
CCCCCHHHHCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHCHHEEEEECCCCHHHHHHHHH
AFIAYNNLSKRIYDKLATLDVEDVKALTAAGKEIRDWVIDAPLQPELEKDIRDAYDTLSK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
DNSGNDVAVAVRSSATAEDLPDASFAGQQETFLNVIGADQVVHKIKEVFASLYNDRAIAY
CCCCCEEEEEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
RVHHGFKHEDVFLSAGIQLMVRSDISASGVLFTLDTESGFREVVFVTASFGLGEMVVQGA
EECCCCCCCCEEEECCEEEEEECCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHCC
VNPDEFYVYKKTLKAGKPAILRRTLGTKLVRMVYSDAPGERVKIEDTPTELRHCFAISDA
CCCHHEEEEHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCC
DVQELARQALIIEQHYGRPMDIEWAKDGISGKLFIVQARPETVKSRSHTTQIERFALQQP
HHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHCCC
GQILTEGRAIGQKIGSGTARVVNSLEDMHHMQPGDVLVADMTDPDWEPVMKRASAIVTNR
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCC
GGRTCHAAIIARELGVPAVVGTGNALSIIQDGQPITVSCAEGDTGYIYAGKLPFERTTTD
CCCCHHHHHHHHHCCCCEEEECCCEEEEEECCCCEEEEECCCCCCEEEECCCCCCCCCCH
LGHMPPAPLKIMMNVANPERAFDFGQLPNAGIGLARLEMIIAAHIGVHPKALLEYHKQDA
HCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCC
DTKQKIDAKIAGYPDPVSFYVNRLAEGIATLTASVAPHTVIVRLSDFKSNEYAHLIGGQH
CHHHHHCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEECCCCC
YEPQEENPMLGFRGASRYVDPSFSDAFALECKAVLKVRNEMGLDNLWVMIPFVRTLEEGR
CCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KVIDVLRQNGLKQGENGLKVIMMCEVPSNALLADEFLDIFDGFSIGSNDLTQLSLGLDRD
HHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCC
SSIVAPLFDERNPAVKKLLSMAIKAARSKGKYIGICGQGPSDHPDLAEWLMQEGIESLSL
CCEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCHHHCCC
NPDTVVDTWLRLAKLKTVH
CCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALNGASYLNENILWLHELRLTDLARVGGKNSSLGEMIGNLTALGVSVPGGYATTAAAFK
CCCCHHHHCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHCHHEEEEECCCCHHHHHHHHH
AFIAYNNLSKRIYDKLATLDVEDVKALTAAGKEIRDWVIDAPLQPELEKDIRDAYDTLSK
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
DNSGNDVAVAVRSSATAEDLPDASFAGQQETFLNVIGADQVVHKIKEVFASLYNDRAIAY
CCCCCEEEEEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
RVHHGFKHEDVFLSAGIQLMVRSDISASGVLFTLDTESGFREVVFVTASFGLGEMVVQGA
EECCCCCCCCEEEECCEEEEEECCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHCC
VNPDEFYVYKKTLKAGKPAILRRTLGTKLVRMVYSDAPGERVKIEDTPTELRHCFAISDA
CCCHHEEEEHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCC
DVQELARQALIIEQHYGRPMDIEWAKDGISGKLFIVQARPETVKSRSHTTQIERFALQQP
HHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHCCC
GQILTEGRAIGQKIGSGTARVVNSLEDMHHMQPGDVLVADMTDPDWEPVMKRASAIVTNR
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCC
GGRTCHAAIIARELGVPAVVGTGNALSIIQDGQPITVSCAEGDTGYIYAGKLPFERTTTD
CCCCHHHHHHHHHCCCCEEEECCCEEEEEECCCCEEEEECCCCCCEEEECCCCCCCCCCH
LGHMPPAPLKIMMNVANPERAFDFGQLPNAGIGLARLEMIIAAHIGVHPKALLEYHKQDA
HCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCC
DTKQKIDAKIAGYPDPVSFYVNRLAEGIATLTASVAPHTVIVRLSDFKSNEYAHLIGGQH
CHHHHHCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEECCCCC
YEPQEENPMLGFRGASRYVDPSFSDAFALECKAVLKVRNEMGLDNLWVMIPFVRTLEEGR
CCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KVIDVLRQNGLKQGENGLKVIMMCEVPSNALLADEFLDIFDGFSIGSNDLTQLSLGLDRD
HHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCC
SSIVAPLFDERNPAVKKLLSMAIKAARSKGKYIGICGQGPSDHPDLAEWLMQEGIESLSL
CCEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCHHHCCC
NPDTVVDTWLRLAKLKTVH
CCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]