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The map label for this gene is prpB

Identifier: 182681067

GI number: 182681067

Start: 616486

End: 617382

Strand: Direct

Name: prpB

Synonym: XfasM23_0506

Alternate gene names: 182681067

Gene position: 616486-617382 (Clockwise)

Preceding gene: 182681066

Following gene: 182681068

Centisome position: 24.31

GC content: 54.74

Gene sequence:

>897_bases
ATGTCCACATCTCTCCCCATCCAGACACCAGGCCAATGTTTCCGTACTGCTTTAATGAACGAGCGCCCCTTGCAAGTGAT
CGGTGCAATGAATGCCTACCACGCACTACTGGCCCAACGTGCCGGTTACCGTGCCCTCTATCTTTCCGGAGGCGGCGTCG
CTGCCGGTTCCCTCGGCCTGCCAGATTTGGGAATCAATACCCTAGAAGATGTCTTGATCGATGTACGCCGCATCACCGAT
GTGTGTGATCTTCCCTTGCTCGTGGATATTGATACGGGTTTCGGTCCCAGCGCACTCAATATCGAACGGACCATCAAGAC
ACTGATTAAAGCTGGTGCTGCTGCGTGCCACATTGAAGACCAAGTAGCGGCAAAACGGTGCGGCCATCGTCCCGGAAAAG
AGATCGTGTCCCTGAGCGATATGGCAGATAGGGTGAAGGCTGCTGCCGATGCCCGCACCGATGCAACCTTCTTTTTGATT
GCCCGGACCGATGCCATTCAGGCCGAAGGTGTGGAAGCGGCGATTGAACGCGCCCAGGCCTATGTCGAAGCTGGCGCTGA
CAGTATTTTTGCCGAGGCCGTCTCCGATTTGGAGACCTACCGACATTTTGTCGAAAGAATCCGAGTCCCCGTGCTTGCCA
ACATGACCGAATTTGGTAAAACCCCACTGTTGACCCGCGAACAATTAGAGGCTGTTGGAGTTGCGATGCAACTTTTCCCA
TTATCCGCATTCCGTGCCGCCAACAAAGCTGCTGAAACCGTGTATATGGCAATTCGCCGTGATGGCCATCAACAGGCTGT
ATTGCCGCACATGCAGACCCGTGCCGAACTATACGAACGCCTTGATTACCATGCCTTCGAACAGCGCTTGGATGCCCTGT
CTTCCCGCAGCACCTGA

Upstream 100 bases:

>100_bases
TAGTGTTCCCCATGATGGAGCACTTCTGAAGGAGCGCTTCCGTGTGGCACCTTAGAGTGCGCTCCTCTCTCCATCTTTTT
TACAGATTAAAAATCGCCCC

Downstream 100 bases:

>100_bases
TCCTTACCTCCTTCCAATGCGGCATCCTAATCGCGCAACGCCAATTTGTCATCGCCATAGCATTGCATTGCAGTGAATAT
TCCGCCTTTCACACCGGATA

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSTSLPIQTPGQCFRTALMNERPLQVIGAMNAYHALLAQRAGYRALYLSGGGVAAGSLGLPDLGINTLEDVLIDVRRITD
VCDLPLLVDIDTGFGPSALNIERTIKTLIKAGAAACHIEDQVAAKRCGHRPGKEIVSLSDMADRVKAAADARTDATFFLI
ARTDAIQAEGVEAAIERAQAYVEAGADSIFAEAVSDLETYRHFVERIRVPVLANMTEFGKTPLLTREQLEAVGVAMQLFP
LSAFRAANKAAETVYMAIRRDGHQQAVLPHMQTRAELYERLDYHAFEQRLDALSSRST

Sequences:

>Translated_298_residues
MSTSLPIQTPGQCFRTALMNERPLQVIGAMNAYHALLAQRAGYRALYLSGGGVAAGSLGLPDLGINTLEDVLIDVRRITD
VCDLPLLVDIDTGFGPSALNIERTIKTLIKAGAAACHIEDQVAAKRCGHRPGKEIVSLSDMADRVKAAADARTDATFFLI
ARTDAIQAEGVEAAIERAQAYVEAGADSIFAEAVSDLETYRHFVERIRVPVLANMTEFGKTPLLTREQLEAVGVAMQLFP
LSAFRAANKAAETVYMAIRRDGHQQAVLPHMQTRAELYERLDYHAFEQRLDALSSRST
>Mature_297_residues
STSLPIQTPGQCFRTALMNERPLQVIGAMNAYHALLAQRAGYRALYLSGGGVAAGSLGLPDLGINTLEDVLIDVRRITDV
CDLPLLVDIDTGFGPSALNIERTIKTLIKAGAAACHIEDQVAAKRCGHRPGKEIVSLSDMADRVKAAADARTDATFFLIA
RTDAIQAEGVEAAIERAQAYVEAGADSIFAEAVSDLETYRHFVERIRVPVLANMTEFGKTPLLTREQLEAVGVAMQLFPL
SAFRAANKAAETVYMAIRRDGHQQAVLPHMQTRAELYERLDYHAFEQRLDALSSRST

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=293, Percent_Identity=62.7986348122867, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1790445, Length=295, Percent_Identity=27.4576271186441, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32427; Mature: 32296

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSLPIQTPGQCFRTALMNERPLQVIGAMNAYHALLAQRAGYRALYLSGGGVAAGSLGL
CCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCC
PDLGINTLEDVLIDVRRITDVCDLPLLVDIDTGFGPSALNIERTIKTLIKAGAAACHIED
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHCCCHH
QVAAKRCGHRPGKEIVSLSDMADRVKAAADARTDATFFLIARTDAIQAEGVEAAIERAQA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHCCHHHHHHHHHH
YVEAGADSIFAEAVSDLETYRHFVERIRVPVLANMTEFGKTPLLTREQLEAVGVAMQLFP
HHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHCCCCCCCHHHHHHHHHHHHHHH
LSAFRAANKAAETVYMAIRRDGHQQAVLPHMQTRAELYERLDYHAFEQRLDALSSRST
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STSLPIQTPGQCFRTALMNERPLQVIGAMNAYHALLAQRAGYRALYLSGGGVAAGSLGL
CCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCC
PDLGINTLEDVLIDVRRITDVCDLPLLVDIDTGFGPSALNIERTIKTLIKAGAAACHIED
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHCCCHH
QVAAKRCGHRPGKEIVSLSDMADRVKAAADARTDATFFLIARTDAIQAEGVEAAIERAQA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHCCHHHHHHHHHH
YVEAGADSIFAEAVSDLETYRHFVERIRVPVLANMTEFGKTPLLTREQLEAVGVAMQLFP
HHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHCCCCCCCHHHHHHHHHHHHHHH
LSAFRAANKAAETVYMAIRRDGHQQAVLPHMQTRAELYERLDYHAFEQRLDALSSRST
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9325432 [H]