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The map label for this gene is greA

Identifier: 182680956

GI number: 182680956

Start: 489011

End: 489487

Strand: Direct

Name: greA

Synonym: XfasM23_0394

Alternate gene names: 182680956

Gene position: 489011-489487 (Clockwise)

Preceding gene: 182680955

Following gene: 182680957

Centisome position: 19.29

GC content: 52.2

Gene sequence:

>477_bases
ATGAGAGCGCCCATGACTTTGAAAGGTGTGCGTCGTCTTCGTGATGAGCTGGAACATCTGAAATCGGTCAAGCGTCCAGA
GATTATCAATGCGATTGCAGAGGCACGTGCGCATGGGGATCTTAAGGAAAATGCTGAATATCACGCTGCGCGTGAACAAC
AAAGCTTTATTGAAGGTCGCATTAAACAGTTGGAAGGCGAGCTTTCGCATGCTGAAGTGATTGATGTCGCCAAACTCAAT
GCGGGTACCAAGATCGTGTTTGGTGCCACGGTGACGTTAGCCGATCTGGGGACTGATGAAGAGAGTCGCTATCAGATCGT
GGGCGATCTTGAGGCTGACATCAAACAGGGTTTGGTTGCGATTTCCTCGCCGGTGGCGCGTGCGCTGATTGGCAAACAAG
AGGGCGATACGATCGTGATTGAGGCGCCTGCTGGCCGACGTGAGTACGAGGTGGTTGCGGTGGAATACATCAGCTGA

Upstream 100 bases:

>100_bases
CGACTACTATTGCTGGCGCTAAGGCGTTGGTGAATTCACTCGAGTTCCGCGGTACCGGCCCGGTTTGGTCGCTACAGGAA
CTCCACAAGGAGCTGCAGGT

Downstream 100 bases:

>100_bases
TTCTGTCATGTCCAGTGTCACGCTGTTGTTGCCGGAGCGTCATTGCTTGCTAGGTACGTTGCGGGGTGACGCGGTTGTGC
GGGCGCTGGCGCGTGCTGAT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MRAPMTLKGVRRLRDELEHLKSVKRPEIINAIAEARAHGDLKENAEYHAAREQQSFIEGRIKQLEGELSHAEVIDVAKLN
AGTKIVFGATVTLADLGTDEESRYQIVGDLEADIKQGLVAISSPVARALIGKQEGDTIVIEAPAGRREYEVVAVEYIS

Sequences:

>Translated_158_residues
MRAPMTLKGVRRLRDELEHLKSVKRPEIINAIAEARAHGDLKENAEYHAAREQQSFIEGRIKQLEGELSHAEVIDVAKLN
AGTKIVFGATVTLADLGTDEESRYQIVGDLEADIKQGLVAISSPVARALIGKQEGDTIVIEAPAGRREYEVVAVEYIS
>Mature_158_residues
MRAPMTLKGVRRLRDELEHLKSVKRPEIINAIAEARAHGDLKENAEYHAAREQQSFIEGRIKQLEGELSHAEVIDVAKLN
AGTKIVFGATVTLADLGTDEESRYQIVGDLEADIKQGLVAISSPVARALIGKQEGDTIVIEAPAGRREYEVVAVEYIS

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=154, Percent_Identity=62.987012987013, Blast_Score=207, Evalue=3e-55,
Organism=Escherichia coli, GI87082261, Length=159, Percent_Identity=33.9622641509434, Blast_Score=90, Evalue=8e-20,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_XYLF2 (B2I844)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829116.1
- ProteinModelPortal:   B2I844
- SMR:   B2I844
- GeneID:   6202674
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0394
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17344; Mature: 17344

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAPMTLKGVRRLRDELEHLKSVKRPEIINAIAEARAHGDLKENAEYHAAREQQSFIEGR
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHH
IKQLEGELSHAEVIDVAKLNAGTKIVFGATVTLADLGTDEESRYQIVGDLEADIKQGLVA
HHHHHCCCCHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCEEEEECCHHHHHHCHHH
ISSPVARALIGKQEGDTIVIEAPAGRREYEVVAVEYIS
HHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEECC
>Mature Secondary Structure
MRAPMTLKGVRRLRDELEHLKSVKRPEIINAIAEARAHGDLKENAEYHAAREQQSFIEGR
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHH
IKQLEGELSHAEVIDVAKLNAGTKIVFGATVTLADLGTDEESRYQIVGDLEADIKQGLVA
HHHHHCCCCHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCEEEEECCHHHHHHCHHH
ISSPVARALIGKQEGDTIVIEAPAGRREYEVVAVEYIS
HHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA