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The map label for this gene is pcl [H]

Identifier: 182680877

GI number: 182680877

Start: 411060

End: 412367

Strand: Direct

Name: pcl [H]

Synonym: XfasM23_0315

Alternate gene names: 182680877

Gene position: 411060-412367 (Clockwise)

Preceding gene: 182680875

Following gene: 182680878

Centisome position: 16.21

GC content: 52.45

Gene sequence:

>1308_bases
ATGGGTGTCGGTTCGCTTCATCGGCCACTGGTATGGGTACATGGGAAGCCTATTACCCTGGGGCGTTTTTTAGCTCAGGT
CCGTGGTTTGGCTGCTCAACTTCCTAGTGGGCGCTATGTGGTGAATTTGTGTGAGGATCGTTACCACTTTTTGTTGGCGT
TCTATGCGTGTGCATTACGTGGCCAGGTTTCTCTTTTACCGTCTTCACGTGCTCCGGGAGTGGTTGCTGAGATTTATGCG
GCTTATCCAGATAGTTACTGTTTGGGTGATCGAGAGTTATCTATGGAGGCGTTGCGTTATTGGCACTTGCCCGAGATCTT
TCCGGAATTAGATGGTGCGATACCTAGTCTTTTGGAAGATGCTGTGGTTGCGATTGGTTTTACTTCTGGAAGTACTGGGG
TGCCTACATCCAATCTCAAAACCTGGGGTAGTTTGGTGACCAGTACGGAGCAGGATTGGGCTGCATTTCATAGTTTGTGG
GGCTCTCAGGTTCCAACGATCGTCGCCACAGTGCCGTCGCAGCATATGTATGGTATGGAGTTTTCGGTGTTGTTGCCGTT
GCTGGCGCCAGTGGCGGTGCATGCTGGTCGGCCATTTTTTCCTGAAGACGTTGCGCAAGCTTTGGCTGAGGTACCAGCAC
CTCGTTTGTTGGTGACGACACCGGTACATCTGCGTGTGTTGGTTCAATCGGGTCTGCGTTTTCCGGAGGTTGCCGGTATT
GTTTCGGCGACTGCGCCACTGGCACATGATTTGGCGGTTGCCGCTGAGGCTTGCTTTGGCTGTGAATTACGGGAGGTATT
TGGTTCTACTGAAACCTGTACTTTTGCGGTGCGCCGTACAGTGTATGAACAAGCATGGACACGGTTTGATGGGGTGAGTA
TTGAGGTCAATGCTGATGTGACTTGGGTGTATGCCCGTCATCTTCCCGCGCCGATGGCTATCGCTGATTTTGTGGATGTG
TACGAGGATGGTCGTTTTGAGGTGCTTGGGCGTCAGGCCGATTTGTTGGAGATCGCCGGTAAGCGTGCTTCGTTGGGTGA
GTTGAATCGGTGTCTATTGGCCATTCCTGGTGTGGTGGATGGTGTGTTGGTGCAGATGCCGCTTGAGGCTGATCAGACGG
TGGGGCGCATTGCTGCGGTGGTGGTCGCGCCGACGTTGCATGAGGTGCATATTCAAAAAATGTTGCGTACAAAAATTGAT
CCGCTTTTTCTGCCGCGTCGTCTACGCAAGGTCGATGCGTTGCCACGTAATGAGACTGGTAAGTTGCCACGCGAGGCGTT
GTTGGCGTTGTTGGCTGATGTGTGTTGA

Upstream 100 bases:

>100_bases
GGTATGGCTGCATGGGGATGATTTCCTTGACTGCTATCTCTACGCTATGTCGCTTGTTGCTTTTCCCTCTTTTCCCTCTT
CAGGTGTCGGGTCTCTTCCG

Downstream 100 bases:

>100_bases
TGAAAATAGTGGGGAATGTGTGTCAGCTTTTTCAAAGGCGAATGGATAGGTGTGTACACACAGGCATGATGTTTAGTGAA
GCGTCGCTGGGAGGGTGATC

Product: putative AMP-dependent synthetase and ligase family protein

Products: NA

Alternate protein names: 4CL; 4-coumaroyl-CoA synthase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MGVGSLHRPLVWVHGKPITLGRFLAQVRGLAAQLPSGRYVVNLCEDRYHFLLAFYACALRGQVSLLPSSRAPGVVAEIYA
AYPDSYCLGDRELSMEALRYWHLPEIFPELDGAIPSLLEDAVVAIGFTSGSTGVPTSNLKTWGSLVTSTEQDWAAFHSLW
GSQVPTIVATVPSQHMYGMEFSVLLPLLAPVAVHAGRPFFPEDVAQALAEVPAPRLLVTTPVHLRVLVQSGLRFPEVAGI
VSATAPLAHDLAVAAEACFGCELREVFGSTETCTFAVRRTVYEQAWTRFDGVSIEVNADVTWVYARHLPAPMAIADFVDV
YEDGRFEVLGRQADLLEIAGKRASLGELNRCLLAIPGVVDGVLVQMPLEADQTVGRIAAVVVAPTLHEVHIQKMLRTKID
PLFLPRRLRKVDALPRNETGKLPREALLALLADVC

Sequences:

>Translated_435_residues
MGVGSLHRPLVWVHGKPITLGRFLAQVRGLAAQLPSGRYVVNLCEDRYHFLLAFYACALRGQVSLLPSSRAPGVVAEIYA
AYPDSYCLGDRELSMEALRYWHLPEIFPELDGAIPSLLEDAVVAIGFTSGSTGVPTSNLKTWGSLVTSTEQDWAAFHSLW
GSQVPTIVATVPSQHMYGMEFSVLLPLLAPVAVHAGRPFFPEDVAQALAEVPAPRLLVTTPVHLRVLVQSGLRFPEVAGI
VSATAPLAHDLAVAAEACFGCELREVFGSTETCTFAVRRTVYEQAWTRFDGVSIEVNADVTWVYARHLPAPMAIADFVDV
YEDGRFEVLGRQADLLEIAGKRASLGELNRCLLAIPGVVDGVLVQMPLEADQTVGRIAAVVVAPTLHEVHIQKMLRTKID
PLFLPRRLRKVDALPRNETGKLPREALLALLADVC
>Mature_434_residues
GVGSLHRPLVWVHGKPITLGRFLAQVRGLAAQLPSGRYVVNLCEDRYHFLLAFYACALRGQVSLLPSSRAPGVVAEIYAA
YPDSYCLGDRELSMEALRYWHLPEIFPELDGAIPSLLEDAVVAIGFTSGSTGVPTSNLKTWGSLVTSTEQDWAAFHSLWG
SQVPTIVATVPSQHMYGMEFSVLLPLLAPVAVHAGRPFFPEDVAQALAEVPAPRLLVTTPVHLRVLVQSGLRFPEVAGIV
SATAPLAHDLAVAAEACFGCELREVFGSTETCTFAVRRTVYEQAWTRFDGVSIEVNADVTWVYARHLPAPMAIADFVDVY
EDGRFEVLGRQADLLEIAGKRASLGELNRCLLAIPGVVDGVLVQMPLEADQTVGRIAAVVVAPTLHEVHIQKMLRTKIDP
LFLPRRLRKVDALPRNETGKLPREALLALLADVC

Specific function: Converts p-coumaric acid into p-coumaryl CoA. This is necessary for the activation of the photoactive yellow protein (PYP) chromophore [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012743
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.12 [H]

Molecular weight: Translated: 47383; Mature: 47252

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVGSLHRPLVWVHGKPITLGRFLAQVRGLAAQLPSGRYVVNLCEDRYHFLLAFYACALR
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHC
GQVSLLPSSRAPGVVAEIYAAYPDSYCLGDRELSMEALRYWHLPEIFPELDGAIPSLLED
CCEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHH
AVVAIGFTSGSTGVPTSNLKTWGSLVTSTEQDWAAFHSLWGSQVPTIVATVPSQHMYGME
HEEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCHHCCCH
FSVLLPLLAPVAVHAGRPFFPEDVAQALAEVPAPRLLVTTPVHLRVLVQSGLRFPEVAGI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCCCCCHHHHH
VSATAPLAHDLAVAAEACFGCELREVFGSTETCTFAVRRTVYEQAWTRFDGVSIEVNADV
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCE
TWVYARHLPAPMAIADFVDVYEDGRFEVLGRQADLLEIAGKRASLGELNRCLLAIPGVVD
EEEEECCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCCHHHHHHHHHHCCCHHC
GVLVQMPLEADQTVGRIAAVVVAPTLHEVHIQKMLRTKIDPLFLPRRLRKVDALPRNETG
CHHEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
KLPREALLALLADVC
CCCHHHHHHHHHHCC
>Mature Secondary Structure 
GVGSLHRPLVWVHGKPITLGRFLAQVRGLAAQLPSGRYVVNLCEDRYHFLLAFYACALR
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHC
GQVSLLPSSRAPGVVAEIYAAYPDSYCLGDRELSMEALRYWHLPEIFPELDGAIPSLLED
CCEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHH
AVVAIGFTSGSTGVPTSNLKTWGSLVTSTEQDWAAFHSLWGSQVPTIVATVPSQHMYGME
HEEEEEECCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCHHCCCH
FSVLLPLLAPVAVHAGRPFFPEDVAQALAEVPAPRLLVTTPVHLRVLVQSGLRFPEVAGI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCCCCCHHHHH
VSATAPLAHDLAVAAEACFGCELREVFGSTETCTFAVRRTVYEQAWTRFDGVSIEVNADV
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCE
TWVYARHLPAPMAIADFVDVYEDGRFEVLGRQADLLEIAGKRASLGELNRCLLAIPGVVD
EEEEECCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCCHHHHHHHHHHCCCHHC
GVLVQMPLEADQTVGRIAAVVVAPTLHEVHIQKMLRTKIDPLFLPRRLRKVDALPRNETG
CHHEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
KLPREALLALLADVC
CCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA