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The map label for this gene is cysS

Identifier: 182680844

GI number: 182680844

Start: 364302

End: 365705

Strand: Reverse

Name: cysS

Synonym: XfasM23_0281

Alternate gene names: 182680844

Gene position: 365705-364302 (Counterclockwise)

Preceding gene: 182680863

Following gene: 182680843

Centisome position: 14.42

GC content: 58.4

Gene sequence:

>1404_bases
ATGAGCCTGCACCTTTACAACACCCTTCACCGGAGAGTCGAGCCGTTCGAACCGCTGGACCCTGCCTGCCCAACTATGTA
TGTATGCGGTCCGACCGTCTACAACTATGTGCATATCGGCAACGCCCGCGGTCCAGTCGTGTTTGGGGTGTTGGCCGCGC
TGCTGCGGCGGCGGTATGGGGCGCTGCGCTACGCACGCAACATCACTGACGTGGATGACAAGATCAACACTGCTGCGCAG
GAGCAGGGTGTTCCCATCTCGGCCATTACTGAACGCTTTTGCGCTGCTTACCGTGAGGATATGAAAAAACTCGGTGTGGA
GCCGCCTGATATCGAGCCGGAAGCCACTGCGCATATGCCACAGATCATCGCGATGATCGAACAGCTGATCGAACGCCAGC
ATGCATATGTCGCGATGGAGCATGTGCTGTTCGCGGTGAACAGCTTTGCCGATTATGGGCAACTGTCGCGCAGGGATACG
GAAGAAATGTTGGCCGGTGCTCGAGTCGAGATCGCTCCATACAAGCGTGACCCAAGCGATTTCGTGCTGTGGAAACCTTC
CAGCGATCAGCTTCCTGGCTGGGACTCGCCCTGGGGGCGCGGCCGCCCCGGCTGGCATATTGAGTGCTCCGCAATGGCCG
CTGCGCATCTGGGGGAGACGATTGATATCCACGCTGGCGGAATCGATCTGCAATTCCCGCATCATGAAAATGAAATCGCA
CAAAGTCGCTGCGCACACGGCAGCAGTACGTTCGCACGGGTCTGGATGCACAACGGGATGCTCAATTTTGAAGGTGCCAA
AATGAGCAAATCGCTGGGCAATATCGAGACCGTGCATGAGTTGGTGGCAAAGCATCCTCCCGAAGCACTACGTTACGCGC
TGCTTTCGGCACATTATCGGAAACCGCTGGACTGGTCAGAGGCACTCATCGCACAGTCGGTACGCACTCTAAACCGGCTG
TATGGGACGCTGCGCGATTTGGCGGCGTATCCGGCACGCGCCATCATCCCAGGGAATATCGAAGCAGCGTTGGATGACGA
TCTCAACACGCCGCAGGCGCTGGCCGAGTTGGCGACACTGGCAAACGAGGCACGCATCCAATTGGCTGACACCACACATC
ACGCAGCAGCCGAGGTCACTACCGCGCTCACCCGGCTGAAAGCCAAGTTGCTTGGCGCCGGACTGGCTCTTGGTCTGCTG
CAGCAAACGCCGGAGGCATGGTTCAGCCAAGGTACCAACGAGAGCGATGAAACGCATATCCAGGCGCTGATTGATGCACG
CGGTGCCGCCAAGCAGGCGCGGGATTTTGTACGTGCCGACGCAATCCGTGCACAACTCGCCGCAGAGGGTATTGTGCTTG
AGGATACACCGCAGGGGGTACGCTGGATGAAGCAGCACACGTAA

Upstream 100 bases:

>100_bases
TACCCCTTCTGGCAGCCAATCACAGTCTCAAGTATGAACCAAAGCATCAAGATACGGTCTCAGGCGCTAGAATCCGAGAT
TGCTCCTAGGATGGTCTGTG

Downstream 100 bases:

>100_bases
AGGTGTGGATCTGACTATCTACGGCAATCACGCTGATACACTCGCCCTACCGTCACCGTACACGGAGTCATCCTGTTCAA
GACCATGACCGATGATCCAT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSLHLYNTLHRRVEPFEPLDPACPTMYVCGPTVYNYVHIGNARGPVVFGVLAALLRRRYGALRYARNITDVDDKINTAAQ
EQGVPISAITERFCAAYREDMKKLGVEPPDIEPEATAHMPQIIAMIEQLIERQHAYVAMEHVLFAVNSFADYGQLSRRDT
EEMLAGARVEIAPYKRDPSDFVLWKPSSDQLPGWDSPWGRGRPGWHIECSAMAAAHLGETIDIHAGGIDLQFPHHENEIA
QSRCAHGSSTFARVWMHNGMLNFEGAKMSKSLGNIETVHELVAKHPPEALRYALLSAHYRKPLDWSEALIAQSVRTLNRL
YGTLRDLAAYPARAIIPGNIEAALDDDLNTPQALAELATLANEARIQLADTTHHAAAEVTTALTRLKAKLLGAGLALGLL
QQTPEAWFSQGTNESDETHIQALIDARGAAKQARDFVRADAIRAQLAAEGIVLEDTPQGVRWMKQHT

Sequences:

>Translated_467_residues
MSLHLYNTLHRRVEPFEPLDPACPTMYVCGPTVYNYVHIGNARGPVVFGVLAALLRRRYGALRYARNITDVDDKINTAAQ
EQGVPISAITERFCAAYREDMKKLGVEPPDIEPEATAHMPQIIAMIEQLIERQHAYVAMEHVLFAVNSFADYGQLSRRDT
EEMLAGARVEIAPYKRDPSDFVLWKPSSDQLPGWDSPWGRGRPGWHIECSAMAAAHLGETIDIHAGGIDLQFPHHENEIA
QSRCAHGSSTFARVWMHNGMLNFEGAKMSKSLGNIETVHELVAKHPPEALRYALLSAHYRKPLDWSEALIAQSVRTLNRL
YGTLRDLAAYPARAIIPGNIEAALDDDLNTPQALAELATLANEARIQLADTTHHAAAEVTTALTRLKAKLLGAGLALGLL
QQTPEAWFSQGTNESDETHIQALIDARGAAKQARDFVRADAIRAQLAAEGIVLEDTPQGVRWMKQHT
>Mature_466_residues
SLHLYNTLHRRVEPFEPLDPACPTMYVCGPTVYNYVHIGNARGPVVFGVLAALLRRRYGALRYARNITDVDDKINTAAQE
QGVPISAITERFCAAYREDMKKLGVEPPDIEPEATAHMPQIIAMIEQLIERQHAYVAMEHVLFAVNSFADYGQLSRRDTE
EMLAGARVEIAPYKRDPSDFVLWKPSSDQLPGWDSPWGRGRPGWHIECSAMAAAHLGETIDIHAGGIDLQFPHHENEIAQ
SRCAHGSSTFARVWMHNGMLNFEGAKMSKSLGNIETVHELVAKHPPEALRYALLSAHYRKPLDWSEALIAQSVRTLNRLY
GTLRDLAAYPARAIIPGNIEAALDDDLNTPQALAELATLANEARIQLADTTHHAAAEVTTALTRLKAKLLGAGLALGLLQ
QTPEAWFSQGTNESDETHIQALIDARGAAKQARDFVRADAIRAQLAAEGIVLEDTPQGVRWMKQHT

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI13375695, Length=388, Percent_Identity=36.5979381443299, Blast_Score=232, Evalue=6e-61,
Organism=Homo sapiens, GI10835051, Length=310, Percent_Identity=32.5806451612903, Blast_Score=182, Evalue=8e-46,
Organism=Homo sapiens, GI21269877, Length=310, Percent_Identity=32.5806451612903, Blast_Score=182, Evalue=8e-46,
Organism=Homo sapiens, GI62240992, Length=310, Percent_Identity=32.5806451612903, Blast_Score=181, Evalue=9e-46,
Organism=Homo sapiens, GI303304987, Length=310, Percent_Identity=32.5806451612903, Blast_Score=181, Evalue=1e-45,
Organism=Escherichia coli, GI1786737, Length=470, Percent_Identity=45.7446808510638, Blast_Score=372, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17509795, Length=295, Percent_Identity=32.2033898305085, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17509797, Length=295, Percent_Identity=32.2033898305085, Blast_Score=163, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324082, Length=317, Percent_Identity=33.1230283911672, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI19922218, Length=500, Percent_Identity=32.8, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI19922364, Length=277, Percent_Identity=38.9891696750903, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e [H]

EC number: =6.1.1.16 [H]

Molecular weight: Translated: 51672; Mature: 51541

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLHLYNTLHRRVEPFEPLDPACPTMYVCGPTVYNYVHIGNARGPVVFGVLAALLRRRYG
CCEEHHHHHHHHCCCCCCCCCCCCEEEEECCHHEEEEEECCCCCHHHHHHHHHHHHHHHH
ALRYARNITDVDDKINTAAQEQGVPISAITERFCAAYREDMKKLGVEPPDIEPEATAHMP
HHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
QIIAMIEQLIERQHAYVAMEHVLFAVNSFADYGQLSRRDTEEMLAGARVEIAPYKRDPSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEECCCCCCCC
FVLWKPSSDQLPGWDSPWGRGRPGWHIECSAMAAAHLGETIDIHAGGIDLQFPHHENEIA
EEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCEEEECCCCHHHHH
QSRCAHGSSTFARVWMHNGMLNFEGAKMSKSLGNIETVHELVAKHPPEALRYALLSAHYR
HHHHCCCCHHHHHHHHHCCEEECCCHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHC
KPLDWSEALIAQSVRTLNRLYGTLRDLAAYPARAIIPGNIEAALDDDLNTPQALAELATL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHCCCCCCCHHHHHHHHHH
ANEARIQLADTTHHAAAEVTTALTRLKAKLLGAGLALGLLQQTPEAWFSQGTNESDETHI
HHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHH
QALIDARGAAKQARDFVRADAIRAQLAAEGIVLEDTPQGVRWMKQHT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHCCC
>Mature Secondary Structure 
SLHLYNTLHRRVEPFEPLDPACPTMYVCGPTVYNYVHIGNARGPVVFGVLAALLRRRYG
CEEHHHHHHHHCCCCCCCCCCCCEEEEECCHHEEEEEECCCCCHHHHHHHHHHHHHHHH
ALRYARNITDVDDKINTAAQEQGVPISAITERFCAAYREDMKKLGVEPPDIEPEATAHMP
HHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
QIIAMIEQLIERQHAYVAMEHVLFAVNSFADYGQLSRRDTEEMLAGARVEIAPYKRDPSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEECCCCCCCC
FVLWKPSSDQLPGWDSPWGRGRPGWHIECSAMAAAHLGETIDIHAGGIDLQFPHHENEIA
EEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCEEEECCCCHHHHH
QSRCAHGSSTFARVWMHNGMLNFEGAKMSKSLGNIETVHELVAKHPPEALRYALLSAHYR
HHHHCCCCHHHHHHHHHCCEEECCCHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHC
KPLDWSEALIAQSVRTLNRLYGTLRDLAAYPARAIIPGNIEAALDDDLNTPQALAELATL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHCCCCCCCHHHHHHHHHH
ANEARIQLADTTHHAAAEVTTALTRLKAKLLGAGLALGLLQQTPEAWFSQGTNESDETHI
HHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHH
QALIDARGAAKQARDFVRADAIRAQLAAEGIVLEDTPQGVRWMKQHT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]