Click here to switch to the map view.

The map label for this gene is nuoB [H]

Identifier: 182680806

GI number: 182680806

Start: 317929

End: 318483

Strand: Direct

Name: nuoB [H]

Synonym: XfasM23_0241

Alternate gene names: 182680806

Gene position: 317929-318483 (Clockwise)

Preceding gene: 182680805

Following gene: 182680807

Centisome position: 12.54

GC content: 53.33

Gene sequence:

>555_bases
ATGGGAGTAATTCAGGCTATTGATCGTCTGATGACTAATCCGATCCCTGATGGTCAGGTGGATGACATTCTACGGCCGCA
GGGGGAGAGTCCACTGTTGCAAAAGGGCTACGTGACAACCAGTGTCGATGCTTTGTTGAACTGGGCGCGTACCGGTTCGA
TGTGGCCAATGACCTTTGGTTTGGCCTGCTGTGCCGTTGAAATGATGCACGCTGGTGCTGCCCGCCTGGATTTGGACCGT
TACGGAATAGTATTTCGTCCTTCGCCGCGTCAGTCTGACGTGATGATCGTGGCGGGTACTCTTGTTAACAAAATGGCTCC
GGCGTTGCGCAAGGTCTACGATCAGATGCCTGACCCGAAGTGGGTGATCTCTATGGGTAGTTGCGCTAATGGTGGCGGTT
ACTATCACTATTCTTATTCAGTGGTGCGCGGTTGTGATCGCATCGTGCCCGTGGATGTCTATGTTCCCGGTTGTCCGCCG
ACTGCCGAAGCGTTGGTCTACGGCATTTTGCAGTTGCAGAAAAAGATCTGGCGAACCAAGACCATCGCAGGTTGA

Upstream 100 bases:

>100_bases
TGTTCATGGATATTGGCGCGCGTTCGCTTGTCACGATGGGGCTATTTGTCGGGATGCTGTTTCTTGGTTTTATATACGTA
TGGAAGAAGGGAGCGCTGGA

Downstream 100 bases:

>100_bases
TCTGTTGTTTTCTATCTACTGAATAGCCAACTATTGAGCAGCCAAGTCCACATGGCAGAACAAACTCTTTCTTTCGTTGA
TTGTCTAATGTCGCGCTTCC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MGVIQAIDRLMTNPIPDGQVDDILRPQGESPLLQKGYVTTSVDALLNWARTGSMWPMTFGLACCAVEMMHAGAARLDLDR
YGIVFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMPDPKWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPP
TAEALVYGILQLQKKIWRTKTIAG

Sequences:

>Translated_184_residues
MGVIQAIDRLMTNPIPDGQVDDILRPQGESPLLQKGYVTTSVDALLNWARTGSMWPMTFGLACCAVEMMHAGAARLDLDR
YGIVFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMPDPKWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPP
TAEALVYGILQLQKKIWRTKTIAG
>Mature_183_residues
GVIQAIDRLMTNPIPDGQVDDILRPQGESPLLQKGYVTTSVDALLNWARTGSMWPMTFGLACCAVEMMHAGAARLDLDRY
GIVFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMPDPKWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPT
AEALVYGILQLQKKIWRTKTIAG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=158, Percent_Identity=74.0506329113924, Blast_Score=256, Evalue=8e-69,
Organism=Escherichia coli, GI1788624, Length=181, Percent_Identity=45.8563535911602, Blast_Score=162, Evalue=1e-41,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=43.4426229508197, Blast_Score=101, Evalue=3e-23,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=41.8032786885246, Blast_Score=95, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=151, Percent_Identity=70.1986754966888, Blast_Score=243, Evalue=5e-65,
Organism=Drosophila melanogaster, GI18859983, Length=173, Percent_Identity=70.5202312138728, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24642371, Length=173, Percent_Identity=70.5202312138728, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24651058, Length=146, Percent_Identity=78.0821917808219, Blast_Score=253, Evalue=5e-68,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 20196; Mature: 20065

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVIQAIDRLMTNPIPDGQVDDILRPQGESPLLQKGYVTTSVDALLNWARTGSMWPMTFG
CCHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCHHHHH
LACCAVEMMHAGAARLDLDRYGIVFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMPDPK
HHHHHHHHHHCCCHHEEHHHCCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCC
WVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGILQLQKKIWRTK
EEEEECCCCCCCCEEEEHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
TIAG
CCCC
>Mature Secondary Structure 
GVIQAIDRLMTNPIPDGQVDDILRPQGESPLLQKGYVTTSVDALLNWARTGSMWPMTFG
CHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCHHHHH
LACCAVEMMHAGAARLDLDRYGIVFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMPDPK
HHHHHHHHHHCCCHHEEHHHCCEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCC
WVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALVYGILQLQKKIWRTK
EEEEECCCCCCCCEEEEHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
TIAG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA