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The map label for this gene is mpl [H]

Identifier: 182680782

GI number: 182680782

Start: 284825

End: 286240

Strand: Direct

Name: mpl [H]

Synonym: XfasM23_0211

Alternate gene names: 182680782

Gene position: 284825-286240 (Clockwise)

Preceding gene: 182680781

Following gene: 182680784

Centisome position: 11.23

GC content: 56.57

Gene sequence:

>1416_bases
ATGAATAAATTGCATATTCTTGGTATTGCCGGAACATTTATGGGGGGCATCGCTGCGCTGGCACGTGAATTGGGCTGGAA
TGTTGCGGGGAGCGATCAGGCCATCTATCCGCCAATGTCAGTGCAATTGGAACGGCTTGGCATCACATTGATGCATGGCT
ATTTGCCGGAGCATATCGCTGCGGATTGTGCTGAGGTGGTGGTAGGGAATGCGCTGTCGCGTGGTAATGCAGCGGTTGAG
GCGTTGCTTGATAGCGGATGCCGTTACATATCGGGGCCCCAATGGTTAGCTGAGCGGGTATTGCCAGGGCGCGACACGCT
TGCGGTGGCAGGAACGCATGGTAAGACGACGACAACGACGATCTTGGCTTGGTTATTGGAGGCAGCCGGACGTGCGCCGG
GATTCCTGATTGGTGGTGTGCCTGAGAATTTTGGTGTGTCAGCGCGGCTTGGCGGTGTGCTGAGCCAGGGGAGTATGGAT
CCGTTGGTGGCGGGTGAGGTGTCTGCAGCTGTTCGACCGTTGTTTGTCGTGGAGGCGGATGAGTACGACACTGCATTCTT
TGATAAGCGCAGCAAATTCGTACATTACCGTCCACGTGTGGTGATTCTGAATAATCTAGAGTACGACCATGCCGATATCT
TCCCTGATGTGGCTGCGATACAACGGCAATTCCACCATTTAGTGCGTACCATCCCTGGTCGTGGTCGGCTGATCGTGAAT
GGTGAGGATCAACGTTTGGCCGAGGTCTTGGCGATGGGGTGTTGGACGCCGGTGGAGCGCTTCGGTTTCGATCCTGAGTT
CCACTGGAGTGCGCGTTTGATTGCTGCCGATGGGAGTCGTTTTTCGGTCTGGCACGAGGGATGTGAGGTCGGTCAGGTCA
GTTGGTCGTTGCTTGGTCGTCACAATGTACTCAACGCGCTGGCGGCGCTGGCGGCTGCACATGCGGTGGGTGTTGCGCCG
GCTGCGGTGATTCCGTCGCTCGCGCAGTTCCGCAGTGTCAAACGTCGCTTGGAATGCTTGGGTGAGAGAGGGGCGGTGAC
GGTTTACGACGACTTTGCACACCACCCCACTGCGATCGCTACCACGCTGGAGGGGTTACGGGCGAATGTGGGTGATGCGC
GTATTGTGGTCGCGATCGAGATGCGCAGTCATTCGATGCGGCTGGGGGCGCATGCGCAGGCCTTGGCGCCGTCGCTACAG
GCGGCTGATACCGTGGTGTTTCTGGATCATCCAGGATTGGTGTGGAACGCAGCAGGCGTGGTTGCACAGATGCATGGTCA
GGTGCATGTGGTTCATGATACTGAGGGGTTACTGTCGATGCTCAATGAGGTGGTACATGCCAGTGACCATGTTGTATTCA
TGTCTAATGGTGGATTTGATGGTGCACCACGTCGATTCTTAGCGCAGTTGCGCTAA

Upstream 100 bases:

>100_bases
TGGAAGTTGGAGTGGGAGCCAGTCGCTGAAATGGCTCTTCTGAAATAGACGTTTATCTGCTGATGTCCGTAGCGTTGCAC
TGATCGGTGACAATGGCCCG

Downstream 100 bases:

>100_bases
GGGGGGGGCTGTGAATGTGTGCAGCGAGAAGCAAGCTACGGTTTTTGTGTAGTGCCTGTGTGATCTGGTGTCTATACTGA
ACGCCTCCGCATCACTATTG

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MNKLHILGIAGTFMGGIAALARELGWNVAGSDQAIYPPMSVQLERLGITLMHGYLPEHIAADCAEVVVGNALSRGNAAVE
ALLDSGCRYISGPQWLAERVLPGRDTLAVAGTHGKTTTTTILAWLLEAAGRAPGFLIGGVPENFGVSARLGGVLSQGSMD
PLVAGEVSAAVRPLFVVEADEYDTAFFDKRSKFVHYRPRVVILNNLEYDHADIFPDVAAIQRQFHHLVRTIPGRGRLIVN
GEDQRLAEVLAMGCWTPVERFGFDPEFHWSARLIAADGSRFSVWHEGCEVGQVSWSLLGRHNVLNALAALAAAHAVGVAP
AAVIPSLAQFRSVKRRLECLGERGAVTVYDDFAHHPTAIATTLEGLRANVGDARIVVAIEMRSHSMRLGAHAQALAPSLQ
AADTVVFLDHPGLVWNAAGVVAQMHGQVHVVHDTEGLLSMLNEVVHASDHVVFMSNGGFDGAPRRFLAQLR

Sequences:

>Translated_471_residues
MNKLHILGIAGTFMGGIAALARELGWNVAGSDQAIYPPMSVQLERLGITLMHGYLPEHIAADCAEVVVGNALSRGNAAVE
ALLDSGCRYISGPQWLAERVLPGRDTLAVAGTHGKTTTTTILAWLLEAAGRAPGFLIGGVPENFGVSARLGGVLSQGSMD
PLVAGEVSAAVRPLFVVEADEYDTAFFDKRSKFVHYRPRVVILNNLEYDHADIFPDVAAIQRQFHHLVRTIPGRGRLIVN
GEDQRLAEVLAMGCWTPVERFGFDPEFHWSARLIAADGSRFSVWHEGCEVGQVSWSLLGRHNVLNALAALAAAHAVGVAP
AAVIPSLAQFRSVKRRLECLGERGAVTVYDDFAHHPTAIATTLEGLRANVGDARIVVAIEMRSHSMRLGAHAQALAPSLQ
AADTVVFLDHPGLVWNAAGVVAQMHGQVHVVHDTEGLLSMLNEVVHASDHVVFMSNGGFDGAPRRFLAQLR
>Mature_471_residues
MNKLHILGIAGTFMGGIAALARELGWNVAGSDQAIYPPMSVQLERLGITLMHGYLPEHIAADCAEVVVGNALSRGNAAVE
ALLDSGCRYISGPQWLAERVLPGRDTLAVAGTHGKTTTTTILAWLLEAAGRAPGFLIGGVPENFGVSARLGGVLSQGSMD
PLVAGEVSAAVRPLFVVEADEYDTAFFDKRSKFVHYRPRVVILNNLEYDHADIFPDVAAIQRQFHHLVRTIPGRGRLIVN
GEDQRLAEVLAMGCWTPVERFGFDPEFHWSARLIAADGSRFSVWHEGCEVGQVSWSLLGRHNVLNALAALAAAHAVGVAP
AAVIPSLAQFRSVKRRLECLGERGAVTVYDDFAHHPTAIATTLEGLRANVGDARIVVAIEMRSHSMRLGAHAQALAPSLQ
AADTVVFLDHPGLVWNAAGVVAQMHGQVHVVHDTEGLLSMLNEVVHASDHVVFMSNGGFDGAPRRFLAQLR

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=469, Percent_Identity=50.1066098081023, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1786279, Length=401, Percent_Identity=30.1745635910224, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50575; Mature: 50575

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLHILGIAGTFMGGIAALARELGWNVAGSDQAIYPPMSVQLERLGITLMHGYLPEHIA
CCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHCCHHHHCCCHHHHH
ADCAEVVVGNALSRGNAAVEALLDSGCRYISGPQWLAERVLPGRDTLAVAGTHGKTTTTT
HHHHHHHHCCCHHCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHH
ILAWLLEAAGRAPGFLIGGVPENFGVSARLGGVLSQGSMDPLVAGEVSAAVRPLFVVEAD
HHHHHHHHCCCCCCEEECCCCCCCCCHHHHCCHHCCCCCCCCHHCCHHHHHCEEEEEECC
EYDTAFFDKRSKFVHYRPRVVILNNLEYDHADIFPDVAAIQRQFHHLVRTIPGRGRLIVN
CCCHHHHHCCCCEEEECCEEEEEECCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCEEEEC
GEDQRLAEVLAMGCWTPVERFGFDPEFHWSARLIAADGSRFSVWHEGCEVGQVSWSLLGR
CCHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEEEECCCEEEHHHCCCCCCCHHHHHHHH
HNVLNALAALAAAHAVGVAPAAVIPSLAQFRSVKRRLECLGERGAVTVYDDFAHHPTAIA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
TTLEGLRANVGDARIVVAIEMRSHSMRLGAHAQALAPSLQAADTVVFLDHPGLVWNAAGV
HHHHHHHCCCCCEEEEEEEEECCCCEECCCHHHHHCCCCCCCCEEEEEECCCCEEEHHHH
VAQMHGQVHVVHDTEGLLSMLNEVVHASDHVVFMSNGGFDGAPRRFLAQLR
HHHHCCCEEEEECCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNKLHILGIAGTFMGGIAALARELGWNVAGSDQAIYPPMSVQLERLGITLMHGYLPEHIA
CCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHCCHHHHCCCHHHHH
ADCAEVVVGNALSRGNAAVEALLDSGCRYISGPQWLAERVLPGRDTLAVAGTHGKTTTTT
HHHHHHHHCCCHHCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHH
ILAWLLEAAGRAPGFLIGGVPENFGVSARLGGVLSQGSMDPLVAGEVSAAVRPLFVVEAD
HHHHHHHHCCCCCCEEECCCCCCCCCHHHHCCHHCCCCCCCCHHCCHHHHHCEEEEEECC
EYDTAFFDKRSKFVHYRPRVVILNNLEYDHADIFPDVAAIQRQFHHLVRTIPGRGRLIVN
CCCHHHHHCCCCEEEECCEEEEEECCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCEEEEC
GEDQRLAEVLAMGCWTPVERFGFDPEFHWSARLIAADGSRFSVWHEGCEVGQVSWSLLGR
CCHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEEEECCCEEEHHHCCCCCCCHHHHHHHH
HNVLNALAALAAAHAVGVAPAAVIPSLAQFRSVKRRLECLGERGAVTVYDDFAHHPTAIA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
TTLEGLRANVGDARIVVAIEMRSHSMRLGAHAQALAPSLQAADTVVFLDHPGLVWNAAGV
HHHHHHHCCCCCEEEEEEEEECCCCEECCCHHHHHCCCCCCCCEEEEEECCCCEEEHHHH
VAQMHGQVHVVHDTEGLLSMLNEVVHASDHVVFMSNGGFDGAPRRFLAQLR
HHHHCCCEEEEECCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]