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The map label for this gene is queD [H]

Identifier: 182680717

GI number: 182680717

Start: 201883

End: 202239

Strand: Direct

Name: queD [H]

Synonym: XfasM23_0146

Alternate gene names: 182680717

Gene position: 201883-202239 (Clockwise)

Preceding gene: 182680716

Following gene: 182680719

Centisome position: 7.96

GC content: 48.46

Gene sequence:

>357_bases
ATGGATATTTTTAAGGTCTTCATTATCGAAGCAGCACACCGTTTGCCTCATGTTCCTCCTGGGCACAAGTGTGCTCGTTT
GCACGGGCATTCTTTTCGAATTGAGGTGCATGTCAGTGGTGCATTAGAGTCTGATACTGGGTGGATCATGGATTTCAGTG
ATTTGAAGCGTGCTTTCCAGCCGATTTACGATCAACTTGACCATCATTACCTCAATGAAATTGAGGGGCTTGAAAATCCA
ACCAGTGAGCGGTTGGCGATTTGGGTATGGGACCGGCTCAAGCCAGTGCTGCCTGCATTGACCGAGATAGTGGTGCATGA
AACCTGTACTTCGGGATGCCGTTACCGTGGTGGTTGA

Upstream 100 bases:

>100_bases
CGGTTCATCAGCGTGGTTGATCTTTGCCTTACATATTGGTAATCACAGCAGAGCATAGTGCCGCTATGCTCTTACTGTAT
TTTCGTTGCATGACAGCACT

Downstream 100 bases:

>100_bases
TTCACCTCATCAAGATATTGTCGTAGGAATGGTGGATCTGTCTCTAATCCCTGGAAAATCGAATTACTGTCATGTAGCAC
GTGCTTCTGAGTTCATATGT

Product: queuosine biosynthesis protein QueD

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: CPH4 synthase; Queuosine biosynthesis protein queD [H]

Number of amino acids: Translated: 118; Mature: 118

Protein sequence:

>118_residues
MDIFKVFIIEAAHRLPHVPPGHKCARLHGHSFRIEVHVSGALESDTGWIMDFSDLKRAFQPIYDQLDHHYLNEIEGLENP
TSERLAIWVWDRLKPVLPALTEIVVHETCTSGCRYRGG

Sequences:

>Translated_118_residues
MDIFKVFIIEAAHRLPHVPPGHKCARLHGHSFRIEVHVSGALESDTGWIMDFSDLKRAFQPIYDQLDHHYLNEIEGLENP
TSERLAIWVWDRLKPVLPALTEIVVHETCTSGCRYRGG
>Mature_118_residues
MDIFKVFIIEAAHRLPHVPPGHKCARLHGHSFRIEVHVSGALESDTGWIMDFSDLKRAFQPIYDQLDHHYLNEIEGLENP
TSERLAIWVWDRLKPVLPALTEIVVHETCTSGCRYRGG

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde [H]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789124, Length=115, Percent_Identity=63.4782608695652, Blast_Score=169, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007116
- InterPro:   IPR007115 [H]

Pfam domain/function: PF01242 PTPS [H]

EC number: 4.2.3.12

Molecular weight: Translated: 13541; Mature: 13541

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIFKVFIIEAAHRLPHVPPGHKCARLHGHSFRIEVHVSGALESDTGWIMDFSDLKRAFQ
CCHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEEECCCEECCCCCEEEHHHHHHHHH
PIYDQLDHHYLNEIEGLENPTSERLAIWVWDRLKPVLPALTEIVVHETCTSGCRYRGG
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDIFKVFIIEAAHRLPHVPPGHKCARLHGHSFRIEVHVSGALESDTGWIMDFSDLKRAFQ
CCHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEEECCCEECCCCCEEEHHHHHHHHH
PIYDQLDHHYLNEIEGLENPTSERLAIWVWDRLKPVLPALTEIVVHETCTSGCRYRGG
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]