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The map label for this gene is rsmE [H]

Identifier: 182680714

GI number: 182680714

Start: 198131

End: 198859

Strand: Direct

Name: rsmE [H]

Synonym: XfasM23_0143

Alternate gene names: 182680714

Gene position: 198131-198859 (Clockwise)

Preceding gene: 182680713

Following gene: 182680715

Centisome position: 7.81

GC content: 52.54

Gene sequence:

>729_bases
TTGAGTCGTTGTCATGTCAATGTGCCCCTGATTCTTGGCAACGAAATAATCCTCCCAGAATTGGTTGCTAACCACCTTTT
GCGTGTGCTGCGTTTATGCGAGGGCGATACCTGTGTACTGTTCAACGGTGACGGCCATGATTATTTAGCACGTCTTATTG
CCACAGGCAGGCGCGAGGCGTGGGCACGCATTGAAGGCGTTTCCGAGGTGTGTAATGAATCACCACTGGCGATTACCCTG
GTCCAAGCAATCACACGCGGTGAGAAAATGGATTTGATTGTGCAAAAGGCAACGGAATTAGGCGTTGTCGCAATCGTGCC
AGTTGATACTGAACGCAGCGGGGTGCGCTTGGATAGCGAACGCGTAGCCAAACGGCTGGCACACTGGAACAACGTGGTCA
TTTCAGCGTGTGAGCAGTGTGGCCGCACGCGCGTGCCAGAAGTACAGATGCCGCGTGCATTGCAAGTGTTGCCGGAAATA
CTTGATCCGGACACGTTGCGCCTGATTCTCGACCCGAAAAGTGAACATCGATTGTCAACGCTATCAAGGCTGACACCAGT
TGAGGTCATCATTGCGATTGGCCCCGAGGGTGGTTGGTCGCCTCGTGATCTTGAAATACTCAAAGATGCAGGTTTTATCG
GTTTTAGCTTGGGGCCGCGTATTTTGCGCACTGAAACAGCAGGATTAACCGCCATCGCCGTGTTGCAAGCACAGCTTGGT
GACCTGTAG

Upstream 100 bases:

>100_bases
ATGTGGTGTATTGGATGCCTCCCTATTGCGTGGATGAGCAGCAACTGCATCTGCTTGCTGAGACCACGCGTGTGGCAATT
GACGAAGCTATCGCATGCGT

Downstream 100 bases:

>100_bases
CGCATATGATTGATGTGGCATCTTGCACTGGAGTTGCATGGCGGCTACAGGTGATCACATGTGCTGCTCTATCGTTGATT
GGTTGTAGCACCGTATGGGC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSRCHVNVPLILGNEIILPELVANHLLRVLRLCEGDTCVLFNGDGHDYLARLIATGRREAWARIEGVSEVCNESPLAITL
VQAITRGEKMDLIVQKATELGVVAIVPVDTERSGVRLDSERVAKRLAHWNNVVISACEQCGRTRVPEVQMPRALQVLPEI
LDPDTLRLILDPKSEHRLSTLSRLTPVEVIIAIGPEGGWSPRDLEILKDAGFIGFSLGPRILRTETAGLTAIAVLQAQLG
DL

Sequences:

>Translated_242_residues
MSRCHVNVPLILGNEIILPELVANHLLRVLRLCEGDTCVLFNGDGHDYLARLIATGRREAWARIEGVSEVCNESPLAITL
VQAITRGEKMDLIVQKATELGVVAIVPVDTERSGVRLDSERVAKRLAHWNNVVISACEQCGRTRVPEVQMPRALQVLPEI
LDPDTLRLILDPKSEHRLSTLSRLTPVEVIIAIGPEGGWSPRDLEILKDAGFIGFSLGPRILRTETAGLTAIAVLQAQLG
DL
>Mature_241_residues
SRCHVNVPLILGNEIILPELVANHLLRVLRLCEGDTCVLFNGDGHDYLARLIATGRREAWARIEGVSEVCNESPLAITLV
QAITRGEKMDLIVQKATELGVVAIVPVDTERSGVRLDSERVAKRLAHWNNVVISACEQCGRTRVPEVQMPRALQVLPEIL
DPDTLRLILDPKSEHRLSTLSRLTPVEVIIAIGPEGGWSPRDLEILKDAGFIGFSLGPRILRTETAGLTAIAVLQAQLGD
L

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=235, Percent_Identity=42.1276595744681, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26520; Mature: 26389

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRCHVNVPLILGNEIILPELVANHLLRVLRLCEGDTCVLFNGDGHDYLARLIATGRREA
CCCEEEECEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCHHH
WARIEGVSEVCNESPLAITLVQAITRGEKMDLIVQKATELGVVAIVPVDTERSGVRLDSE
HHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEEECCCCCCCEECHH
RVAKRLAHWNNVVISACEQCGRTRVPEVQMPRALQVLPEILDPDTLRLILDPKSEHRLST
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LSRLTPVEVIIAIGPEGGWSPRDLEILKDAGFIGFSLGPRILRTETAGLTAIAVLQAQLG
HHHCCCEEEEEEECCCCCCCCCHHHHHHHCCEEEECCCCEEEEECCCCHHHHHHHHHHHC
DL
CC
>Mature Secondary Structure 
SRCHVNVPLILGNEIILPELVANHLLRVLRLCEGDTCVLFNGDGHDYLARLIATGRREA
CCEEEECEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCHHH
WARIEGVSEVCNESPLAITLVQAITRGEKMDLIVQKATELGVVAIVPVDTERSGVRLDSE
HHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEEECCCCCCCEECHH
RVAKRLAHWNNVVISACEQCGRTRVPEVQMPRALQVLPEILDPDTLRLILDPKSEHRLST
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LSRLTPVEVIIAIGPEGGWSPRDLEILKDAGFIGFSLGPRILRTETAGLTAIAVLQAQLG
HHHCCCEEEEEEECCCCCCCCCHHHHHHHCCEEEECCCCEEEEECCCCHHHHHHHHHHHC
DL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]