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The map label for this gene is exoA [H]

Identifier: 182680692

GI number: 182680692

Start: 163901

End: 164701

Strand: Direct

Name: exoA [H]

Synonym: XfasM23_0121

Alternate gene names: 182680692

Gene position: 163901-164701 (Clockwise)

Preceding gene: 182680691

Following gene: 182680693

Centisome position: 6.46

GC content: 48.19

Gene sequence:

>801_bases
TTGAGAATTTCCAGTTTTAATGCCAATGGGTTGCGATCGGCTCTTAGTAAGGGTTTTTTTGATTGGTTTTCTACTCAGGA
CATTGATGTGCTGTGTGTGCAGGAAATCAAGGCGCAGAGGCACCAGCTAGTTGGACCGGATTTTTTTCCGGCTGGCTATC
ATGTGTGTTTTCGGGATGCGACGATTAAGAAAGGTTATAGCGGGGTGGCGATTTACAGTAGGTGTGAGCCGGATGAGCTT
CGCACTACGCTGGGTTGGGCCGAGTTTGACGAAGAGGGCCGTTATGTGGAGGCGCGATTCGGAAATTTGAGTGTGGTTTC
GTTTTACATTCCTTCTGGTTCTTCAGGGGAGAAGCGTCAGCATTATAAGTTCGAGGTCATGCGTTGGTTGCGTCCGATTT
TGTGCGAGTGGTTGCTCAGTGGTCGGGATTATGTGTTGTGTGGTGATTGGAATATTGTGCGTAGTGCATTGGATATCCGT
AATTGGAAATCTAATCAGAAAAATTCCGGTTGTTTGCCGTTGGAGCGCGATTGGCTCAATGGTTTATGTGCCGATTCTCT
ACATGATGTTAATTCGGCTCAGGGGCGAGGCTGGGTTGATACTTACCGTGTGTTGCATCCGCAAGGTGAGGATTACACGT
GGTGGAGTAACCGCGGTGCGGCGCGCGTTAATAATGTGGGATGGCGGATTGATTATCAGTTTGTTTCTCCAGGTTTGCGT
GAACGTGTGTCGCGTTGTGCGATTTATTGTGAGCAACGTTTTTCAGATCATGCCCCTTACACTGTGGAGTATGTGTTGTG
A

Upstream 100 bases:

>100_bases
TAAGTCATCAGTGTGATGATGATGTGAGTTTCTGGAAAAGTAGTTATGCATTTGTGATGTCTTAACTATTGAATGAAAAA
AATATGAATTTGGGTGATTT

Downstream 100 bases:

>100_bases
GTGACTATTCGGGAAAGTTCGATGTGCATGCTGTTTTGCAAAATGGACGGCGTTTTCTGACTGTGTTGTTGCTTGGTTTT
GCCTCGGGATTGCCGCTTGC

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MRISSFNANGLRSALSKGFFDWFSTQDIDVLCVQEIKAQRHQLVGPDFFPAGYHVCFRDATIKKGYSGVAIYSRCEPDEL
RTTLGWAEFDEEGRYVEARFGNLSVVSFYIPSGSSGEKRQHYKFEVMRWLRPILCEWLLSGRDYVLCGDWNIVRSALDIR
NWKSNQKNSGCLPLERDWLNGLCADSLHDVNSAQGRGWVDTYRVLHPQGEDYTWWSNRGAARVNNVGWRIDYQFVSPGLR
ERVSRCAIYCEQRFSDHAPYTVEYVL

Sequences:

>Translated_266_residues
MRISSFNANGLRSALSKGFFDWFSTQDIDVLCVQEIKAQRHQLVGPDFFPAGYHVCFRDATIKKGYSGVAIYSRCEPDEL
RTTLGWAEFDEEGRYVEARFGNLSVVSFYIPSGSSGEKRQHYKFEVMRWLRPILCEWLLSGRDYVLCGDWNIVRSALDIR
NWKSNQKNSGCLPLERDWLNGLCADSLHDVNSAQGRGWVDTYRVLHPQGEDYTWWSNRGAARVNNVGWRIDYQFVSPGLR
ERVSRCAIYCEQRFSDHAPYTVEYVL
>Mature_266_residues
MRISSFNANGLRSALSKGFFDWFSTQDIDVLCVQEIKAQRHQLVGPDFFPAGYHVCFRDATIKKGYSGVAIYSRCEPDEL
RTTLGWAEFDEEGRYVEARFGNLSVVSFYIPSGSSGEKRQHYKFEVMRWLRPILCEWLLSGRDYVLCGDWNIVRSALDIR
NWKSNQKNSGCLPLERDWLNGLCADSLHDVNSAQGRGWVDTYRVLHPQGEDYTWWSNRGAARVNNVGWRIDYQFVSPGLR
ERVSRCAIYCEQRFSDHAPYTVEYVL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=268, Percent_Identity=33.2089552238806, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI18375503, Length=268, Percent_Identity=33.2089552238806, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI18375501, Length=268, Percent_Identity=33.2089552238806, Blast_Score=138, Evalue=5e-33,
Organism=Escherichia coli, GI1788046, Length=278, Percent_Identity=28.7769784172662, Blast_Score=81, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71989536, Length=261, Percent_Identity=27.5862068965517, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI221330655, Length=269, Percent_Identity=33.457249070632, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI17136678, Length=269, Percent_Identity=33.457249070632, Blast_Score=127, Evalue=8e-30,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30798; Mature: 30798

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISSFNANGLRSALSKGFFDWFSTQDIDVLCVQEIKAQRHQLVGPDFFPAGYHVCFRDA
CCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
TIKKGYSGVAIYSRCEPDELRTTLGWAEFDEEGRYVEARFGNLSVVSFYIPSGSSGEKRQ
HHHCCCCCEEEEECCCHHHHHHHHCHHHCCCCCCEEEEECCCEEEEEEECCCCCCCCCHH
HYKFEVMRWLRPILCEWLLSGRDYVLCGDWNIVRSALDIRNWKSNQKNSGCLPLERDWLN
HHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHC
GLCADSLHDVNSAQGRGWVDTYRVLHPQGEDYTWWSNRGAARVNNVGWRIDYQFVSPGLR
CHHHHHHHCCCCCCCCCCHHHHHEECCCCCCCEEECCCCCCEECCCCEEEEEHHHCCHHH
ERVSRCAIYCEQRFSDHAPYTVEYVL
HHHHHHHHHHHHHCCCCCCEEEEEEC
>Mature Secondary Structure
MRISSFNANGLRSALSKGFFDWFSTQDIDVLCVQEIKAQRHQLVGPDFFPAGYHVCFRDA
CCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
TIKKGYSGVAIYSRCEPDELRTTLGWAEFDEEGRYVEARFGNLSVVSFYIPSGSSGEKRQ
HHHCCCCCEEEEECCCHHHHHHHHCHHHCCCCCCEEEEECCCEEEEEEECCCCCCCCCHH
HYKFEVMRWLRPILCEWLLSGRDYVLCGDWNIVRSALDIRNWKSNQKNSGCLPLERDWLN
HHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHC
GLCADSLHDVNSAQGRGWVDTYRVLHPQGEDYTWWSNRGAARVNNVGWRIDYQFVSPGLR
CHHHHHHHCCCCCCCCCCHHHHHEECCCCCCCEEECCCCCCEECCCCEEEEEHHHCCHHH
ERVSRCAIYCEQRFSDHAPYTVEYVL
HHHHHHHHHHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]