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The map label for this gene is pyrE

Identifier: 182680686

GI number: 182680686

Start: 155176

End: 155835

Strand: Reverse

Name: pyrE

Synonym: XfasM23_0115

Alternate gene names: 182680686

Gene position: 155835-155176 (Counterclockwise)

Preceding gene: 182680688

Following gene: 182680685

Centisome position: 6.15

GC content: 53.79

Gene sequence:

>660_bases
ATGTCTCACTACCGCCAACGTTTCCTACAGCTGGCTTTAGACTCAAACGCGCTGTGCTTCGGCGAATTCACGCTCAAATC
CGGCCGCATCAGTCCCTATTTCTTCAACGCTGGACACTTTAATTCAGGCGCAAAAACTGCTGCCTTAGCACAGTGTTACG
CCGACGCAATTGATGCGGCGAATATGAACTTTGACCTGGTGTTTGGTCCAGCCTACAAAGGGATTCCACTCGCTACAGCA
CTGGCCTGCGAGTACGCACGGCGTGAGCGTGACCTACTCCTCACATTCAATCGTAAAGAAGTCAAAAATCACGGGGAGGG
TGGCACTCTCATCGGCGCGCCACTGAACGGACGCAAGATATTGATTATCGACGACGTCATCACCGCAGGCACAGCAATCC
GAGAGGCATTACGTATCATCCGCAACGCTGGAGGTACCCCAACGGGGATTGCCGTAGCCCTGAATCGCCAGGAAATCGCT
TCAGAGACCAACCGCCAATCGTCCGTTCAAGCACTGATGGCCGAAACCGGGATCCCTGTCGTAGCGATCGCAACCCTGAG
CGACCTGTTAGCATTTGTAGAAGAGAACGCCAGTTTGGCCAAGTTTTACGAGCCTCTGCTGGCCTACAAGACCCACTATG
GGACCGAAGCATCTGACTGA

Upstream 100 bases:

>100_bases
AGAACACCATCCGAAAAGTCCCTCTTGGCACGATAGAGAAGACCGCGCCAGTGGCTATCCTTACAGCTTTATCCTCTCCA
CATGACTTAGTGACCCCTCT

Downstream 100 bases:

>100_bases
ACAAGCAAAAAAGTGACCTGCGATGTCCATAAAAAAACACCACATCTACTGGAACACCATCTTATTTGTTGCCGTTGCCG
CATGGCCGTGGCCTGGCAAC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSHYRQRFLQLALDSNALCFGEFTLKSGRISPYFFNAGHFNSGAKTAALAQCYADAIDAANMNFDLVFGPAYKGIPLATA
LACEYARRERDLLLTFNRKEVKNHGEGGTLIGAPLNGRKILIIDDVITAGTAIREALRIIRNAGGTPTGIAVALNRQEIA
SETNRQSSVQALMAETGIPVVAIATLSDLLAFVEENASLAKFYEPLLAYKTHYGTEASD

Sequences:

>Translated_219_residues
MSHYRQRFLQLALDSNALCFGEFTLKSGRISPYFFNAGHFNSGAKTAALAQCYADAIDAANMNFDLVFGPAYKGIPLATA
LACEYARRERDLLLTFNRKEVKNHGEGGTLIGAPLNGRKILIIDDVITAGTAIREALRIIRNAGGTPTGIAVALNRQEIA
SETNRQSSVQALMAETGIPVVAIATLSDLLAFVEENASLAKFYEPLLAYKTHYGTEASD
>Mature_218_residues
SHYRQRFLQLALDSNALCFGEFTLKSGRISPYFFNAGHFNSGAKTAALAQCYADAIDAANMNFDLVFGPAYKGIPLATAL
ACEYARRERDLLLTFNRKEVKNHGEGGTLIGAPLNGRKILIIDDVITAGTAIREALRIIRNAGGTPTGIAVALNRQEIAS
ETNRQSSVQALMAETGIPVVAIATLSDLLAFVEENASLAKFYEPLLAYKTHYGTEASD

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=187, Percent_Identity=28.3422459893048, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1790073, Length=216, Percent_Identity=47.2222222222222, Blast_Score=199, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI17508631, Length=149, Percent_Identity=33.5570469798658, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323530, Length=200, Percent_Identity=47, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6323927, Length=199, Percent_Identity=46.7336683417085, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_XYLF2 (B2I6N0)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828846.1
- ProteinModelPortal:   B2I6N0
- SMR:   B2I6N0
- GeneID:   6203496
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0115
- HOGENOM:   HBG404341
- OMA:   FAFNRKE
- ProtClustDB:   PRK00455
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 23677; Mature: 23546

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 26-26 BINDING 98-98 BINDING 99-99 BINDING 102-102 BINDING 104-104 BINDING 128-128 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHYRQRFLQLALDSNALCFGEFTLKSGRISPYFFNAGHFNSGAKTAALAQCYADAIDAA
CCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCC
NMNFDLVFGPAYKGIPLATALACEYARRERDLLLTFNRKEVKNHGEGGTLIGAPLNGRKI
CCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCEEEECCCCCCEE
LIIDDVITAGTAIREALRIIRNAGGTPTGIAVALNRQEIASETNRQSSVQALMAETGIPV
EEECCHHHCCHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHCHHHHHHHHHHHCCCCE
VAIATLSDLLAFVEENASLAKFYEPLLAYKTHYGTEASD
EHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SHYRQRFLQLALDSNALCFGEFTLKSGRISPYFFNAGHFNSGAKTAALAQCYADAIDAA
CHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCC
NMNFDLVFGPAYKGIPLATALACEYARRERDLLLTFNRKEVKNHGEGGTLIGAPLNGRKI
CCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCEEEECCCCCCEE
LIIDDVITAGTAIREALRIIRNAGGTPTGIAVALNRQEIASETNRQSSVQALMAETGIPV
EEECCHHHCCHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHCHHHHHHHHHHHCCCCE
VAIATLSDLLAFVEENASLAKFYEPLLAYKTHYGTEASD
EHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA