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The map label for this gene is trmD

Identifier: 182680646

GI number: 182680646

Start: 105327

End: 106112

Strand: Direct

Name: trmD

Synonym: XfasM23_0075

Alternate gene names: 182680646

Gene position: 105327-106112 (Clockwise)

Preceding gene: 182680645

Following gene: 182680647

Centisome position: 4.15

GC content: 53.05

Gene sequence:

>786_bases
ATGCGTATTGACGTTATCAGCTTGTTCCCCGAGTTCATTAAACAGTCTGTCGGGTTTGGCGTGATCGGTAGAGCACAGGA
ACGTGGTTTGCTTGATTTACACAACTGGAATCCACGCGACTATGCTCAAGGAAATTACCGGCGTGTCGATGATCGTCCGT
TCGGTGGTGGTCCAGGGATGGTGATGCTGATCGAGCCACTGCGTGCCTGCCTTGAAGCAGTACGGGCAGCAGATCCTCAA
CCAGCGCCCTTGATTTATCTCAGTCCGCAAGGTGTCCTGCTGAACCAGTCCCGCGCGAGGAAGCTGGCGATGCTGCCGCG
CATGATATTGCTATGCGGGCGATACGAAGGGATTGACGAGCGTTTTATCGCCCATGAAGTAAACATGGAGTTGTCAATTG
GCGACTATGTGTTGTCTGGCGGTGAATTGGGAGCGGCTGTGGTTGTCGACGCAGTCACCCGGTTACAGGAGGGGGTATTG
AACGACGCTGAGTCAGCCAAACAGGACAGCTTTGAAGCAGCCGATAGCCTGTTTGATTATCCCCACTACACTCATCCATC
CAATCATGCTTTTGGGAATGTTCCCGAAGTATTACGCTCAGGTAACCATGTTGCGATCACGCGTTGGCGTCGCCAGCAGT
CGCTGTTGCGCACTTGGTTGCGGCGACCAGACCTAATAGATGAAGCGAGACTGTCGAAAGCTGATCGCTTACTGCTTGAT
GAGATTAAGCGCACACATCCTATGGATACGGATCGAAAAGCGTCAGCCTCTTGGCGTGGAGCATAG

Upstream 100 bases:

>100_bases
CGTGATGGCGTCCATGATCAGGCCAGTTCTGTCCCGTCTTGCCGGATACTGTTTCACCACAGCATCCCTCATCCAATCCG
GTTTCAGATATCCCGCTTGC

Downstream 100 bases:

>100_bases
CCATTATGCTAAGATCCGGCTCCCTTACGCAGACATCGCGTTTTTTGTTTTCATTGGTCTGCGAGCTGATGTAATCATGT
GGTACAACCCGGTGGCTACA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRIDVISLFPEFIKQSVGFGVIGRAQERGLLDLHNWNPRDYAQGNYRRVDDRPFGGGPGMVMLIEPLRACLEAVRAADPQ
PAPLIYLSPQGVLLNQSRARKLAMLPRMILLCGRYEGIDERFIAHEVNMELSIGDYVLSGGELGAAVVVDAVTRLQEGVL
NDAESAKQDSFEAADSLFDYPHYTHPSNHAFGNVPEVLRSGNHVAITRWRRQQSLLRTWLRRPDLIDEARLSKADRLLLD
EIKRTHPMDTDRKASASWRGA

Sequences:

>Translated_261_residues
MRIDVISLFPEFIKQSVGFGVIGRAQERGLLDLHNWNPRDYAQGNYRRVDDRPFGGGPGMVMLIEPLRACLEAVRAADPQ
PAPLIYLSPQGVLLNQSRARKLAMLPRMILLCGRYEGIDERFIAHEVNMELSIGDYVLSGGELGAAVVVDAVTRLQEGVL
NDAESAKQDSFEAADSLFDYPHYTHPSNHAFGNVPEVLRSGNHVAITRWRRQQSLLRTWLRRPDLIDEARLSKADRLLLD
EIKRTHPMDTDRKASASWRGA
>Mature_261_residues
MRIDVISLFPEFIKQSVGFGVIGRAQERGLLDLHNWNPRDYAQGNYRRVDDRPFGGGPGMVMLIEPLRACLEAVRAADPQ
PAPLIYLSPQGVLLNQSRARKLAMLPRMILLCGRYEGIDERFIAHEVNMELSIGDYVLSGGELGAAVVVDAVTRLQEGVL
NDAESAKQDSFEAADSLFDYPHYTHPSNHAFGNVPEVLRSGNHVAITRWRRQQSLLRTWLRRPDLIDEARLSKADRLLLD
EIKRTHPMDTDRKASASWRGA

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=246, Percent_Identity=51.6260162601626, Blast_Score=240, Evalue=5e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_XYLF2 (B2I6A8)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828806.1
- ProteinModelPortal:   B2I6A8
- SMR:   B2I6A8
- GeneID:   6202592
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0075
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 29353; Mature: 29353

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDVISLFPEFIKQSVGFGVIGRAQERGLLDLHNWNPRDYAQGNYRRVDDRPFGGGPGM
CCCHHHHHHHHHHHHHCCCCEECCCCCCCCEECCCCCCCCCCCCCCEECCCCCCCCCCCH
VMLIEPLRACLEAVRAADPQPAPLIYLSPQGVLLNQSRARKLAMLPRMILLCGRYEGIDE
HHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEECHHHHHHHHHHHHHHHHHCCCCCCHH
RFIAHEVNMELSIGDYVLSGGELGAAVVVDAVTRLQEGVLNDAESAKQDSFEAADSLFDY
HHHHHHCCEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
PHYTHPSNHAFGNVPEVLRSGNHVAITRWRRQQSLLRTWLRRPDLIDEARLSKADRLLLD
CCCCCCCCCCCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EIKRTHPMDTDRKASASWRGA
HHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRIDVISLFPEFIKQSVGFGVIGRAQERGLLDLHNWNPRDYAQGNYRRVDDRPFGGGPGM
CCCHHHHHHHHHHHHHCCCCEECCCCCCCCEECCCCCCCCCCCCCCEECCCCCCCCCCCH
VMLIEPLRACLEAVRAADPQPAPLIYLSPQGVLLNQSRARKLAMLPRMILLCGRYEGIDE
HHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEECHHHHHHHHHHHHHHHHHCCCCCCHH
RFIAHEVNMELSIGDYVLSGGELGAAVVVDAVTRLQEGVLNDAESAKQDSFEAADSLFDY
HHHHHHCCEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
PHYTHPSNHAFGNVPEVLRSGNHVAITRWRRQQSLLRTWLRRPDLIDEARLSKADRLLLD
CCCCCCCCCCCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EIKRTHPMDTDRKASASWRGA
HHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA