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The map label for this gene is waaA [H]

Identifier: 182680642

GI number: 182680642

Start: 101073

End: 102419

Strand: Direct

Name: waaA [H]

Synonym: XfasM23_0071

Alternate gene names: 182680642

Gene position: 101073-102419 (Clockwise)

Preceding gene: 182680640

Following gene: 182680643

Centisome position: 3.99

GC content: 54.05

Gene sequence:

>1347_bases
ATGCATGCGGTCATCGGGTTCCGTATCCTGGTATGCATGCGGAAAGACCCGATTGAATGGCTGTTGCGCGCACTATATTC
GGCGCTGTTGTATTTATTACTGCCCTTTACCATTTACCACTTGGTCTGGCGCGGTTTCCGGGTCCGTCAATATTTCAACC
GATGGAATGAACGCTATGCTTCATATCCAGCAGCGTATGCCCGTCCTCAGGTGTGGTTACATGCTGTTTCGGTCGGTGAG
GTCAATGCTGCTGCCTCGTTGGTGAATGCGTTGCGCCAACAACGGCCGGATATCCGTTGGGTCATCACCACCATTACCCC
CACAGGATCTGAGCGGGTGCGTGCCCTCTGGGGGGATTCATTGGAGCATGTGTATTTGCCATACGATGTGCCCGGCAGTG
TGGAACGTTTTCTGCGCTATTTTCGTCCGCAATTAGCGCTGATTTTGGAAACGGAGCTGTGGCCGAATTTGCTGTTCGGT
TGCCGCAGTCATGGCATTCCTGTGTATATCCTCAATGCACGGCTGTCGGTACGTTCGTTGCGTGGTTATCGTTTGCTCAA
GCCGCTCATCAGGCGTGCGTTACGTTCTGTGGTGTGTGTGGCGGCGCAATCGCAAGAGGATGCACTGCGCTTTTTGCGTC
TGGGTGCGCGTCCCGATCAAGTGGTTGCATTAGGTAATTTGAAGTTCGATATCCCCACACCACAGGATCTTGATGTTTTC
ATGGCTGCGTTTCGCAGGTGTGTTCCGATGGCGCGTCCGGTGTGGATTGCGGCCAGTACGCATGAAGGCGAGGAGGCTGC
GATCATCGACATCCACACGCGCTTACTACGTCGTATCCCGAATTTGTTGCTGTTGTGGGCACCACGGCACCCGGAGCGTT
TTCATAAGGTTGAAGGGCTGGCATACGAACACGGTTGGCGTGTCAGTACGCGCAAGCAACACACGTGGCCAGATGTGACA
ACCGACGTGTTTGTCATTGATACCCTTGGTGAGCTGAGTGCATTTTACGGTTGTGCTCAGGTTGCTTTTGTTGGTGGCAG
TTTGCAGCCAATCGGAGGCCATAATTTGCTGGAGCCAGCCGCTGTTGGTACCGCTACAGTGACTGGGCCGTATTTGCACA
ATTTCTCCGAAATCTCGCGGCGCATGAAGGGTGCTCAAGCGTTGGAAATCTGTGCCGATGCTGAAGCGGTCGGCGCGATG
TTGGAACATCTACTGGCAGATCCAAGCAAGCGTAGCCAGATGGCCCAGGCTGGGTTGGCGTTAGTTGCTAACGGCCGTGG
TGCTGTTGCGCGCACATTGGTGCAGATCAATCCTCATTTGCCATCAATGATCTCTGTCGAGAAGTGA

Upstream 100 bases:

>100_bases
GCCAGTGTGCTGGTTTCAGTAGGGATGGACGGGGAGTCACGTTTGTAGAATCTGACATAGCGTAATTCTATGGGATCGGC
TTGAGTATGTTCTCTGATAG

Downstream 100 bases:

>100_bases
GCGCGGCTTGGTTGCTGCGTTCAAAAATGTTAACTCATGCGTATTCGAGCTCATTTGAGCCATTGGCTCGAATCATATGG
CTCTGAGAAAAACACTGATC

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MHAVIGFRILVCMRKDPIEWLLRALYSALLYLLLPFTIYHLVWRGFRVRQYFNRWNERYASYPAAYARPQVWLHAVSVGE
VNAAASLVNALRQQRPDIRWVITTITPTGSERVRALWGDSLEHVYLPYDVPGSVERFLRYFRPQLALILETELWPNLLFG
CRSHGIPVYILNARLSVRSLRGYRLLKPLIRRALRSVVCVAAQSQEDALRFLRLGARPDQVVALGNLKFDIPTPQDLDVF
MAAFRRCVPMARPVWIAASTHEGEEAAIIDIHTRLLRRIPNLLLLWAPRHPERFHKVEGLAYEHGWRVSTRKQHTWPDVT
TDVFVIDTLGELSAFYGCAQVAFVGGSLQPIGGHNLLEPAAVGTATVTGPYLHNFSEISRRMKGAQALEICADAEAVGAM
LEHLLADPSKRSQMAQAGLALVANGRGAVARTLVQINPHLPSMISVEK

Sequences:

>Translated_448_residues
MHAVIGFRILVCMRKDPIEWLLRALYSALLYLLLPFTIYHLVWRGFRVRQYFNRWNERYASYPAAYARPQVWLHAVSVGE
VNAAASLVNALRQQRPDIRWVITTITPTGSERVRALWGDSLEHVYLPYDVPGSVERFLRYFRPQLALILETELWPNLLFG
CRSHGIPVYILNARLSVRSLRGYRLLKPLIRRALRSVVCVAAQSQEDALRFLRLGARPDQVVALGNLKFDIPTPQDLDVF
MAAFRRCVPMARPVWIAASTHEGEEAAIIDIHTRLLRRIPNLLLLWAPRHPERFHKVEGLAYEHGWRVSTRKQHTWPDVT
TDVFVIDTLGELSAFYGCAQVAFVGGSLQPIGGHNLLEPAAVGTATVTGPYLHNFSEISRRMKGAQALEICADAEAVGAM
LEHLLADPSKRSQMAQAGLALVANGRGAVARTLVQINPHLPSMISVEK
>Mature_448_residues
MHAVIGFRILVCMRKDPIEWLLRALYSALLYLLLPFTIYHLVWRGFRVRQYFNRWNERYASYPAAYARPQVWLHAVSVGE
VNAAASLVNALRQQRPDIRWVITTITPTGSERVRALWGDSLEHVYLPYDVPGSVERFLRYFRPQLALILETELWPNLLFG
CRSHGIPVYILNARLSVRSLRGYRLLKPLIRRALRSVVCVAAQSQEDALRFLRLGARPDQVVALGNLKFDIPTPQDLDVF
MAAFRRCVPMARPVWIAASTHEGEEAAIIDIHTRLLRRIPNLLLLWAPRHPERFHKVEGLAYEHGWRVSTRKQHTWPDVT
TDVFVIDTLGELSAFYGCAQVAFVGGSLQPIGGHNLLEPAAVGTATVTGPYLHNFSEISRRMKGAQALEICADAEAVGAM
LEHLLADPSKRSQMAQAGLALVANGRGAVARTLVQINPHLPSMISVEK

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=421, Percent_Identity=44.8931116389549, Blast_Score=346, Evalue=2e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 50523; Mature: 50523

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAVIGFRILVCMRKDPIEWLLRALYSALLYLLLPFTIYHLVWRGFRVRQYFNRWNERYA
CCCHHEEHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYPAAYARPQVWLHAVSVGEVNAAASLVNALRQQRPDIRWVITTITPTGSERVRALWGDS
CCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHCCC
LEHVYLPYDVPGSVERFLRYFRPQLALILETELWPNLLFGCRSHGIPVYILNARLSVRSL
CCEEEECCCCCHHHHHHHHHHCCHHEEEEEHHHHHHHHHHHHCCCCCEEEEECCCHHHHH
RGYRLLKPLIRRALRSVVCVAAQSQEDALRFLRLGARPDQVVALGNLKFDIPTPQDLDVF
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEECCEEEECCCCCCHHHH
MAAFRRCVPMARPVWIAASTHEGEEAAIIDIHTRLLRRIPNLLLLWAPRHPERFHKVEGL
HHHHHHHCCCCCCEEEEEECCCCCCEEEEHHHHHHHHHCCCEEEEECCCCHHHHHHHCCC
AYEHGWRVSTRKQHTWPDVTTDVFVIDTLGELSAFYGCAQVAFVGGSLQPIGGHNLLEPA
EECCCCEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCH
AVGTATVTGPYLHNFSEISRRMKGAQALEICADAEAVGAMLEHLLADPSKRSQMAQAGLA
HCCCEECCCHHHHCHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHCCEE
LVANGRGAVARTLVQINPHLPSMISVEK
EEECCCCHHHHHHEEECCCCCCCCCCCC
>Mature Secondary Structure
MHAVIGFRILVCMRKDPIEWLLRALYSALLYLLLPFTIYHLVWRGFRVRQYFNRWNERYA
CCCHHEEHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYPAAYARPQVWLHAVSVGEVNAAASLVNALRQQRPDIRWVITTITPTGSERVRALWGDS
CCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHCCC
LEHVYLPYDVPGSVERFLRYFRPQLALILETELWPNLLFGCRSHGIPVYILNARLSVRSL
CCEEEECCCCCHHHHHHHHHHCCHHEEEEEHHHHHHHHHHHHCCCCCEEEEECCCHHHHH
RGYRLLKPLIRRALRSVVCVAAQSQEDALRFLRLGARPDQVVALGNLKFDIPTPQDLDVF
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEECCEEEECCCCCCHHHH
MAAFRRCVPMARPVWIAASTHEGEEAAIIDIHTRLLRRIPNLLLLWAPRHPERFHKVEGL
HHHHHHHCCCCCCEEEEEECCCCCCEEEEHHHHHHHHHCCCEEEEECCCCHHHHHHHCCC
AYEHGWRVSTRKQHTWPDVTTDVFVIDTLGELSAFYGCAQVAFVGGSLQPIGGHNLLEPA
EECCCCEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCH
AVGTATVTGPYLHNFSEISRRMKGAQALEICADAEAVGAMLEHLLADPSKRSQMAQAGLA
HCCCEECCCHHHHCHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHCCEE
LVANGRGAVARTLVQINPHLPSMISVEK
EEECCCCHHHHHHEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]