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The map label for this gene is kgtP [H]

Identifier: 182680627

GI number: 182680627

Start: 85213

End: 86520

Strand: Reverse

Name: kgtP [H]

Synonym: XfasM23_0056

Alternate gene names: 182680627

Gene position: 86520-85213 (Counterclockwise)

Preceding gene: 182680628

Following gene: 182680626

Centisome position: 3.41

GC content: 51.53

Gene sequence:

>1308_bases
ATGTCCGACAGCCTCTCCACTGCACTTTCACACTACACAGCAGAGGAGAAGCGCCATCGCATTTTCGCGATCATCGCCGT
TTCCTCCGGCAACTTAGTCGAATGGTTCGACTTTTACGTCTACGCATTCACCGCGCTGTATTTTTCCTCCCAGTTTTTCC
CTGAGTCCAATCAAACCGCACAATTACTCAACACTGCCGCAATCTTTGGTGCAGGCTTCTTGATGCGGCCTATCGGCGGC
TGGCTGTTCGGCCGGATTGCCGATCGCTACGGACGCAAACGCTCGTTATTGATCTCGGTAATGATGATGTGTGGCGGTTC
AATGGTGATCGCCACGCTTCCTACCCATCATATGATCGGTGCCTGGGCGGCGTTTTTATTACTACTCGCACGTCTGATAC
AGGGTCTGTCGGTAGGTGGTGAGTACGGTTCTACCGCAACTTACATGAGTGAAATTGCGCTGCGTGGACGACGCGGCTTC
TATTCCTCGTTCCAGTACGTCACGTTGATCGGCGGCCAGTTGCTAGCAGTGTTGACTCTGGTGGTGCTGGAAGCTTTATT
CACTGAAGTTCAAATGCGCGCCTGGGGTTGGCGTATTCCATTCATGATCGGTGCGATTGCCGCAGCAGTTGCGTTATTAC
TACGCCGCACACTGAACGAAACTCAATCAGAGGATAACCGACATGACACCCATGCTGGTTCACTATCAACCCTGTTCCGC
GAGCACCGCACTGCATTCCTAACCGTATTTGGATATACCGCTGGCGGCTCGCTGATTTTTTACACTTTCACCACCTATAT
GCAGAAGTTCCTAGTCAACAGTTCCGGCTTATCCAAACAGACTGCTAGTCTGGTGATGAGTGGCGCACTGTTTGTCTATA
TGTGCATTCAACCATTGTTCGGCCTACTGTCGGACTATATCGGGCGGCGCAACAACATGCTGCTATTTGGTGGGTTAGGT
GCGGTCTGTACTGTACCGATCCTCGCAACATTACAGCGTATTCATTCACCACTACCCGCATTTATATTGATCGTAGGAGC
GTTGTCGATTATCAGCTTCTACACCTCCATCTCCGCCATCGTGAAAGCCGAGATGTTCCCAGCAAAAGTACGGGCGCTAG
GTGTGGGCTTCGCCTACGCAGTAGGTAACGCCATATTTGGTGGTTCGGCCGAATATATTGCCCTGGGACTGAAGAGCTTC
GATCTAGAAACGGTGTTCTTCTGGTACGTGGCAGCAATGATGGCGGTCGTATTCTTGGTCAGTTTGCGCCTACCACGGCA
AGCCACGTACCTGCATCATGACCATTAA

Upstream 100 bases:

>100_bases
TGATGCTTGGACTCCAACTCTGCACTCAAAGTATGTATCCGCCGAATACTAGGTGCTACCAAACTTAAAACTTTTGACAG
CATTACGGAGCGCCCTTTTG

Downstream 100 bases:

>100_bases
AACATGCACCGATTTTATAGAAGACAATCAAGTTCAGTTCAACGAAAACAACTACCCTCCTCTTTAATGCGTACCGAAAA
TGCCAACCGACCAGCAGATC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSDSLSTALSHYTAEEKRHRIFAIIAVSSGNLVEWFDFYVYAFTALYFSSQFFPESNQTAQLLNTAAIFGAGFLMRPIGG
WLFGRIADRYGRKRSLLISVMMMCGGSMVIATLPTHHMIGAWAAFLLLLARLIQGLSVGGEYGSTATYMSEIALRGRRGF
YSSFQYVTLIGGQLLAVLTLVVLEALFTEVQMRAWGWRIPFMIGAIAAAVALLLRRTLNETQSEDNRHDTHAGSLSTLFR
EHRTAFLTVFGYTAGGSLIFYTFTTYMQKFLVNSSGLSKQTASLVMSGALFVYMCIQPLFGLLSDYIGRRNNMLLFGGLG
AVCTVPILATLQRIHSPLPAFILIVGALSIISFYTSISAIVKAEMFPAKVRALGVGFAYAVGNAIFGGSAEYIALGLKSF
DLETVFFWYVAAMMAVVFLVSLRLPRQATYLHHDH

Sequences:

>Translated_435_residues
MSDSLSTALSHYTAEEKRHRIFAIIAVSSGNLVEWFDFYVYAFTALYFSSQFFPESNQTAQLLNTAAIFGAGFLMRPIGG
WLFGRIADRYGRKRSLLISVMMMCGGSMVIATLPTHHMIGAWAAFLLLLARLIQGLSVGGEYGSTATYMSEIALRGRRGF
YSSFQYVTLIGGQLLAVLTLVVLEALFTEVQMRAWGWRIPFMIGAIAAAVALLLRRTLNETQSEDNRHDTHAGSLSTLFR
EHRTAFLTVFGYTAGGSLIFYTFTTYMQKFLVNSSGLSKQTASLVMSGALFVYMCIQPLFGLLSDYIGRRNNMLLFGGLG
AVCTVPILATLQRIHSPLPAFILIVGALSIISFYTSISAIVKAEMFPAKVRALGVGFAYAVGNAIFGGSAEYIALGLKSF
DLETVFFWYVAAMMAVVFLVSLRLPRQATYLHHDH
>Mature_434_residues
SDSLSTALSHYTAEEKRHRIFAIIAVSSGNLVEWFDFYVYAFTALYFSSQFFPESNQTAQLLNTAAIFGAGFLMRPIGGW
LFGRIADRYGRKRSLLISVMMMCGGSMVIATLPTHHMIGAWAAFLLLLARLIQGLSVGGEYGSTATYMSEIALRGRRGFY
SSFQYVTLIGGQLLAVLTLVVLEALFTEVQMRAWGWRIPFMIGAIAAAVALLLRRTLNETQSEDNRHDTHAGSLSTLFRE
HRTAFLTVFGYTAGGSLIFYTFTTYMQKFLVNSSGLSKQTASLVMSGALFVYMCIQPLFGLLSDYIGRRNNMLLFGGLGA
VCTVPILATLQRIHSPLPAFILIVGALSIISFYTSISAIVKAEMFPAKVRALGVGFAYAVGNAIFGGSAEYIALGLKSFD
LETVFFWYVAAMMAVVFLVSLRLPRQATYLHHDH

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=427, Percent_Identity=64.1686182669789, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1790550, Length=386, Percent_Identity=32.6424870466321, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1788292, Length=382, Percent_Identity=31.6753926701571, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1789941, Length=409, Percent_Identity=31.7848410757946, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87082231, Length=455, Percent_Identity=26.5934065934066, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082404, Length=398, Percent_Identity=23.3668341708543, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47919; Mature: 47788

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSLSTALSHYTAEEKRHRIFAIIAVSSGNLVEWFDFYVYAFTALYFSSQFFPESNQTA
CCCHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
QLLNTAAIFGAGFLMRPIGGWLFGRIADRYGRKRSLLISVMMMCGGSMVIATLPTHHMIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCHHHHH
AWAAFLLLLARLIQGLSVGGEYGSTATYMSEIALRGRRGFYSSFQYVTLIGGQLLAVLTL
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VVLEALFTEVQMRAWGWRIPFMIGAIAAAVALLLRRTLNETQSEDNRHDTHAGSLSTLFR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
EHRTAFLTVFGYTAGGSLIFYTFTTYMQKFLVNSSGLSKQTASLVMSGALFVYMCIQPLF
HHHHEEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GLLSDYIGRRNNMLLFGGLGAVCTVPILATLQRIHSPLPAFILIVGALSIISFYTSISAI
HHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VKAEMFPAKVRALGVGFAYAVGNAIFGGSAEYIALGLKSFDLETVFFWYVAAMMAVVFLV
HHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHEEEECHHCCHHHHHHHHHHHHHHHHHHH
SLRLPRQATYLHHDH
HHCCCCHHCCCCCCC
>Mature Secondary Structure 
SDSLSTALSHYTAEEKRHRIFAIIAVSSGNLVEWFDFYVYAFTALYFSSQFFPESNQTA
CCHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
QLLNTAAIFGAGFLMRPIGGWLFGRIADRYGRKRSLLISVMMMCGGSMVIATLPTHHMIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCHHHHH
AWAAFLLLLARLIQGLSVGGEYGSTATYMSEIALRGRRGFYSSFQYVTLIGGQLLAVLTL
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VVLEALFTEVQMRAWGWRIPFMIGAIAAAVALLLRRTLNETQSEDNRHDTHAGSLSTLFR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
EHRTAFLTVFGYTAGGSLIFYTFTTYMQKFLVNSSGLSKQTASLVMSGALFVYMCIQPLF
HHHHEEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GLLSDYIGRRNNMLLFGGLGAVCTVPILATLQRIHSPLPAFILIVGALSIISFYTSISAI
HHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VKAEMFPAKVRALGVGFAYAVGNAIFGGSAEYIALGLKSFDLETVFFWYVAAMMAVVFLV
HHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHEEEECHHCCHHHHHHHHHHHHHHHHHHH
SLRLPRQATYLHHDH
HHCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]