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The map label for this gene is ecpD [H]

Identifier: 182680624

GI number: 182680624

Start: 81611

End: 82411

Strand: Reverse

Name: ecpD [H]

Synonym: XfasM23_0053

Alternate gene names: 182680624

Gene position: 82411-81611 (Counterclockwise)

Preceding gene: 182680625

Following gene: 182680623

Centisome position: 3.25

GC content: 48.69

Gene sequence:

>801_bases
ATGGTTCCTCCGATCATGAGAACTCAACAAAGCAAAAGCATCTGGGTGCTTGCCCTAAGCACGTGCTTATGCGCCCAATC
CGCTTTCGCCGGCATCATCATCGCTGGGACCAGGGTTGTCTATCCCGCACAATCACGTGATGTCACCGTACAAGTGACCA
ACACTGGGGAAAACCCTGCACTGTTACAAACCTGGATGGATGACGGCGACTCCAAGCAAACACCTGACGAAAGTAAAGCC
CCCTTTCTGGTGACGCCTCCTATCAGTCGAGTCGAACCTGGATCAGGCCAAGCGTTGCGTTTATTTTTTACGGGCACCAC
AACGCTTCCAGAAGATCGGGAAAGCTTATTCTGGTTAAACGTTCTCGAGATTCCACCTACTCCGTTAGAATATTCCGATA
AAAATGCTCCAACTTCCGCTGATAAATCAGACAGCCAGAACTACCTGCAACTCGCATTTCGCTCGCGGATCAAGGTGTTC
TATCGTCCATCTAAACTCAAAGGAACAGCAAACGAAGCGCCCGCCTCGCTCAGTTGGAAAGTGAATTCCAACGGGCAATT
GCTAGTGAGCAACCCCACACCCTATCACGTCACGCTCACCCGAACCGAAGCGCTGGATGACAAAGGAAGGAAAGAGACAA
TTGACAAAATTGGAGTGTTGTTAACCCCTGGCCAGGAATACACATTCAAAGCGAACAAATCGGATGGACTCTCATCACGG
TGGAAAAGTGTGTCTTTTACCTACATTAATGATTATGGCGGAATAAAAACGCTCAGCAGTAATTTGGGATCAATGCCTTA
A

Upstream 100 bases:

>100_bases
ACATTCAGTACACCATCATCTACCAATAATCGATAACGACGCACTGGGGTGACGTACGTCGCTCCGTGCGTCATCCTGCC
CTGCACTTTATTTCATTCAT

Downstream 100 bases:

>100_bases
CAGCGCTATTAATAATAAAAAATACTATGAAAAAGCAGGAAATATCGCAACGGAGGAAGGTTGACTCCCTTCAGCTCAGT
CGCGTGGAATTCGTCTCAAA

Product: Pili assembly chaperone, N-terminal

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MVPPIMRTQQSKSIWVLALSTCLCAQSAFAGIIIAGTRVVYPAQSRDVTVQVTNTGENPALLQTWMDDGDSKQTPDESKA
PFLVTPPISRVEPGSGQALRLFFTGTTTLPEDRESLFWLNVLEIPPTPLEYSDKNAPTSADKSDSQNYLQLAFRSRIKVF
YRPSKLKGTANEAPASLSWKVNSNGQLLVSNPTPYHVTLTRTEALDDKGRKETIDKIGVLLTPGQEYTFKANKSDGLSSR
WKSVSFTYINDYGGIKTLSSNLGSMP

Sequences:

>Translated_266_residues
MVPPIMRTQQSKSIWVLALSTCLCAQSAFAGIIIAGTRVVYPAQSRDVTVQVTNTGENPALLQTWMDDGDSKQTPDESKA
PFLVTPPISRVEPGSGQALRLFFTGTTTLPEDRESLFWLNVLEIPPTPLEYSDKNAPTSADKSDSQNYLQLAFRSRIKVF
YRPSKLKGTANEAPASLSWKVNSNGQLLVSNPTPYHVTLTRTEALDDKGRKETIDKIGVLLTPGQEYTFKANKSDGLSSR
WKSVSFTYINDYGGIKTLSSNLGSMP
>Mature_266_residues
MVPPIMRTQQSKSIWVLALSTCLCAQSAFAGIIIAGTRVVYPAQSRDVTVQVTNTGENPALLQTWMDDGDSKQTPDESKA
PFLVTPPISRVEPGSGQALRLFFTGTTTLPEDRESLFWLNVLEIPPTPLEYSDKNAPTSADKSDSQNYLQLAFRSRIKVF
YRPSKLKGTANEAPASLSWKVNSNGQLLVSNPTPYHVTLTRTEALDDKGRKETIDKIGVLLTPGQEYTFKANKSDGLSSR
WKSVSFTYINDYGGIKTLSSNLGSMP

Specific function: Probably mediates assembly of a pilin [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=249, Percent_Identity=40.5622489959839, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1789532, Length=243, Percent_Identity=37.8600823045267, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1788428, Length=240, Percent_Identity=33.75, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1790771, Length=246, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787171, Length=255, Percent_Identity=32.5490196078431, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786743, Length=198, Percent_Identity=34.3434343434343, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1786936, Length=233, Percent_Identity=32.1888412017167, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87081806, Length=242, Percent_Identity=27.6859504132231, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI87082203, Length=261, Percent_Identity=27.2030651340996, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788677, Length=222, Percent_Identity=28.3783783783784, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 29226; Mature: 29226

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPPIMRTQQSKSIWVLALSTCLCAQSAFAGIIIAGTRVVYPAQSRDVTVQVTNTGENPA
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHCEEEEECCEEEECCCCCCEEEEEECCCCCCC
LLQTWMDDGDSKQTPDESKAPFLVTPPISRVEPGSGQALRLFFTGTTTLPEDRESLFWLN
EEEEECCCCCCCCCCCCCCCCEEECCCHHHCCCCCCCEEEEEEECCCCCCCCCCCEEEEE
VLEIPPTPLEYSDKNAPTSADKSDSQNYLQLAFRSRIKVFYRPSKLKGTANEAPASLSWK
EEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCCEEEEE
VNSNGQLLVSNPTPYHVTLTRTEALDDKGRKETIDKIGVLLTPGQEYTFKANKSDGLSSR
ECCCCEEEECCCCCEEEEEEECCCCCCCCHHHHHHHCCEEEECCCCEEEECCCCCCCCHH
WKSVSFTYINDYGGIKTLSSNLGSMP
HCEEEEEEEECCCCHHHHHHHCCCCC
>Mature Secondary Structure
MVPPIMRTQQSKSIWVLALSTCLCAQSAFAGIIIAGTRVVYPAQSRDVTVQVTNTGENPA
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHCEEEEECCEEEECCCCCCEEEEEECCCCCCC
LLQTWMDDGDSKQTPDESKAPFLVTPPISRVEPGSGQALRLFFTGTTTLPEDRESLFWLN
EEEEECCCCCCCCCCCCCCCCEEECCCHHHCCCCCCCEEEEEEECCCCCCCCCCCEEEEE
VLEIPPTPLEYSDKNAPTSADKSDSQNYLQLAFRSRIKVFYRPSKLKGTANEAPASLSWK
EEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCCEEEEE
VNSNGQLLVSNPTPYHVTLTRTEALDDKGRKETIDKIGVLLTPGQEYTFKANKSDGLSSR
ECCCCEEEECCCCCEEEEEEECCCCCCCCHHHHHHHCCEEEECCCCEEEECCCCCCCCHH
WKSVSFTYINDYGGIKTLSSNLGSMP
HCEEEEEEEECCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8102362; 8202364; 9278503 [H]