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The map label for this gene is afr [H]

Identifier: 182677619

GI number: 182677619

Start: 706949

End: 707953

Strand: Direct

Name: afr [H]

Synonym: Bind_0626

Alternate gene names: 182677619

Gene position: 706949-707953 (Clockwise)

Preceding gene: 182677618

Following gene: 182677620

Centisome position: 16.95

GC content: 51.64

Gene sequence:

>1005_bases
ATGACAAAGACAGTTGGGTGGGGCTTGATTGGAGCCAGCAATGTTGCTCGTGAATGGATCATCGATGCCATTAGAGCTCA
ACCTGATAATGAAATTGTTACAGTATTGAGCAGTGATAATGATCGCGCGGCCAACTATGCCGCGGAAAATAATATTCCTC
ACCATTGCACGCAACTCGAAGCTCTTTTATCCGACGACCGGATAGACGCCGTTTATATCAGCACCACAAATGAGCTCCAT
AAACAGCAGGCGCTCGCGGCGGCAGCTGCAGGCAAGCATATTTTGTGTGAAAAGCCTCTTGCTCTCAGCCTGAATGATGC
CCGTGATATGATTGATGCTTGCCGTCGCGCTGGCGTCATTATGGCGACCAATCATCATCTGCGCAACGCTGCGACCCATC
ATGAAATCCGCAGGATGTTGCGGGATAATGTGATTGGAAAGCCGCTTTTTATCAGGGTCTTCCACGCCAATTACCTGCCT
CCTCATCTGCAAGGATGGCGCGTGAATAACGCGGGCGGTGGAGTGATTTTGGATAGCACGATTCACGATATCGATACATT
ACGGTTTCTTCTTGATGCGGAGCCGGTCAGCGTGACTGCGATGACCCAGAGCGCTCTTTTTACCGCCGGTGATCAGGAGG
ATGGCGTCATGGCTATAGTGCGCTTTGATAATGGTGCGATTGCCCAGATTCATGGTGCCTATTCCGTCAAGGTCGCACCC
GGTGGCATCGAAATCTATAGTACCGACAAGGCGATTGTGGCGCGCAATGTGATGAGCCAGCGGGCGATAGGACAGGTTTT
TCTGAGGGACGCTGAAGGTGAGCACGAAATCCCGATTGTTCATGAGAACCTCTATCATCGTGCGCTTCGCGCTTTTAATA
AGGCGCTTCGCAAAGAGGGGGAGCCCGCCGCTACTGGTGAAGATGGATTGCGGTCTTTGGCCGTAGCATTAGCAATAAAA
GAAGCGGCGCAGACCGGCTGTCAAAGGGAGGTTCAATACGCATGA

Upstream 100 bases:

>100_bases
TCGTTAAAGTGTTGTATTCCAGCCGTTTGATTGCGTGTCATGGAGCTGGAATGAAGAGAGATGGTGTCGATCGTCAGCAA
GCAGAGGCGAGTGAGGAAAG

Downstream 100 bases:

>100_bases
CATCGTCATCCTTAACCTACACAATAAAAAAGGGTACCGGTATGGAAGATGCGTCAATTACACAAAGCCATGCCGCTACT
ATACAAAAATCAATTGTTAA

Product: oxidoreductase domain-containing protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTKTVGWGLIGASNVAREWIIDAIRAQPDNEIVTVLSSDNDRAANYAAENNIPHHCTQLEALLSDDRIDAVYISTTNELH
KQQALAAAAAGKHILCEKPLALSLNDARDMIDACRRAGVIMATNHHLRNAATHHEIRRMLRDNVIGKPLFIRVFHANYLP
PHLQGWRVNNAGGGVILDSTIHDIDTLRFLLDAEPVSVTAMTQSALFTAGDQEDGVMAIVRFDNGAIAQIHGAYSVKVAP
GGIEIYSTDKAIVARNVMSQRAIGQVFLRDAEGEHEIPIVHENLYHRALRAFNKALRKEGEPAATGEDGLRSLAVALAIK
EAAQTGCQREVQYA

Sequences:

>Translated_334_residues
MTKTVGWGLIGASNVAREWIIDAIRAQPDNEIVTVLSSDNDRAANYAAENNIPHHCTQLEALLSDDRIDAVYISTTNELH
KQQALAAAAAGKHILCEKPLALSLNDARDMIDACRRAGVIMATNHHLRNAATHHEIRRMLRDNVIGKPLFIRVFHANYLP
PHLQGWRVNNAGGGVILDSTIHDIDTLRFLLDAEPVSVTAMTQSALFTAGDQEDGVMAIVRFDNGAIAQIHGAYSVKVAP
GGIEIYSTDKAIVARNVMSQRAIGQVFLRDAEGEHEIPIVHENLYHRALRAFNKALRKEGEPAATGEDGLRSLAVALAIK
EAAQTGCQREVQYA
>Mature_333_residues
TKTVGWGLIGASNVAREWIIDAIRAQPDNEIVTVLSSDNDRAANYAAENNIPHHCTQLEALLSDDRIDAVYISTTNELHK
QQALAAAAAGKHILCEKPLALSLNDARDMIDACRRAGVIMATNHHLRNAATHHEIRRMLRDNVIGKPLFIRVFHANYLPP
HLQGWRVNNAGGGVILDSTIHDIDTLRFLLDAEPVSVTAMTQSALFTAGDQEDGVMAIVRFDNGAIAQIHGAYSVKVAPG
GIEIYSTDKAIVARNVMSQRAIGQVFLRDAEGEHEIPIVHENLYHRALRAFNKALRKEGEPAATGEDGLRSLAVALAIKE
AAQTGCQREVQYA

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=235, Percent_Identity=21.7021276595745, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI87082405, Length=178, Percent_Identity=29.2134831460674, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787574, Length=350, Percent_Identity=25.1428571428571, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081947, Length=201, Percent_Identity=25.3731343283582, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693182, Length=220, Percent_Identity=25, Blast_Score=62, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581117, Length=232, Percent_Identity=24.5689655172414, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24581115, Length=195, Percent_Identity=25.6410256410256, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 36465; Mature: 36334

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTVGWGLIGASNVAREWIIDAIRAQPDNEIVTVLSSDNDRAANYAAENNIPHHCTQLE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHCHHHCCCCCHHHHHHH
ALLSDDRIDAVYISTTNELHKQQALAAAAAGKHILCEKPLALSLNDARDMIDACRRAGVI
HHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCCEEECHHHHHHHHHHHHCCEE
MATNHHLRNAATHHEIRRMLRDNVIGKPLFIRVFHANYLPPHLQGWRVNNAGGGVILDST
EEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCEEECCH
IHDIDTLRFLLDAEPVSVTAMTQSALFTAGDQEDGVMAIVRFDNGAIAQIHGAYSVKVAP
HHHHHHHHHHHCCCCCEEEEEEHHHEEECCCCCCCEEEEEEECCCCEEEEECEEEEEECC
GGIEIYSTDKAIVARNVMSQRAIGQVFLRDAEGEHEIPIVHENLYHRALRAFNKALRKEG
CCEEEEECCHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHCC
EPAATGEDGLRSLAVALAIKEAAQTGCQREVQYA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TKTVGWGLIGASNVAREWIIDAIRAQPDNEIVTVLSSDNDRAANYAAENNIPHHCTQLE
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHCHHHCCCCCHHHHHHH
ALLSDDRIDAVYISTTNELHKQQALAAAAAGKHILCEKPLALSLNDARDMIDACRRAGVI
HHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCCEEECHHHHHHHHHHHHCCEE
MATNHHLRNAATHHEIRRMLRDNVIGKPLFIRVFHANYLPPHLQGWRVNNAGGGVILDST
EEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCEEECCH
IHDIDTLRFLLDAEPVSVTAMTQSALFTAGDQEDGVMAIVRFDNGAIAQIHGAYSVKVAP
HHHHHHHHHHHCCCCCEEEEEEHHHEEECCCCCCCEEEEEEECCCCEEEEECEEEEEECC
GGIEIYSTDKAIVARNVMSQRAIGQVFLRDAEGEHEIPIVHENLYHRALRAFNKALRKEG
CCEEEEECCHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHCC
EPAATGEDGLRSLAVALAIKEAAQTGCQREVQYA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA