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The map label for this gene is thcD [H]

Identifier: 182677561

GI number: 182677561

Start: 628327

End: 629898

Strand: Direct

Name: thcD [H]

Synonym: Bind_0566

Alternate gene names: 182677561

Gene position: 628327-629898 (Clockwise)

Preceding gene: 182677560

Following gene: 182677568

Centisome position: 15.07

GC content: 60.5

Gene sequence:

>1572_bases
ATGCCGTCCAACGAGCAAGAAAATGCGGCAGTAAACACTCAAAAAACATGTGCCAAACCGCTGTTCATTGATCTGACGCA
TCCTGTCAAACTCGAAACGATCCCCGAAAACCAGATTGTCGAAGCCTGTCTTGGCGAGCAAAGCGCGATCCTGATCCGCG
AGGGGACGACGCTTTCGATCCTGAGCGGCAAATGCACCCATGCTGGCGGCCCGCTCGCCGAAGGGCTTTTAATGGAGGGG
CATATACGCTGCCCTTGGCATCATGCCTGTTTCGATGCGCATACGGGCGAAGCTTTGGCCGCGCCCGCCTTTGATCCGCT
CGAGCGCTGGGCCGCGCGGATTGAGGACGGCATGGTGTTCGTCGATCACAAAGACAGTACCCCGACCCATGCCTTGCAAG
CGGCGGCACCAAGCGATCCCTTCGTGATCGTCGGCGGTGGTGCGGCAGGATTTGCGGCAGCCGAGGCGCTTTACCATGAG
GGCTTTGTCGGTCAGGTCGTGCTGATCTCACGCGAGGCCGCGGGTCCCTATGACCGCACGATGCTGAGCAAGGATTATCT
GGGCGGCAAGCTCGGCGAGGATCAATTGCCCTTGCATAGCCAGGCTTTCGGTCAGGGCTCCGGCAAATCCGCTGGCCGGA
TCGATCTCGGCACCGAGGTCGAGCGGATTGATCGCGGCCGCCATTGCGTGGTCTTCGCCGATGGCCAGGAATTGCGTTAT
GCCAAGCTGCTCTTGGCCACTGGCGCCGAGCCACGCGCGCCGACCTTCCCGGGCTATAACCTGCCGCATGTCCATGTCCT
GCGTTCGCTTGCCGATTGCCGATCCTTGATCGAGCATGCGGCCCATGCGCGTCATGTGGTTGTTTTGGGCGCGAGTTTCA
TCGGGCTCGAGGCGGCGGCGAGTTTGCGGGAACGCGGGCTGGAGGTCGATATCGTCGCCCATAGCGAACAGCCGATGGAG
AAAACCCTTGGTGCTCAATTGGGGGAGACTCTGCGGGCCCTGCATGAGAATCACGGCAATCGCTTTCATCTCGGCCGTAC
CATTCTCTCGGTAGAACCAGACCGTGTCCTGCTCGATGACGGCACGGTGCTTCCGGCCGATCTGGTTCTTGTCGGGATCG
GGGTGGAGCCGCGGCTCGATCTCGCCAAGGCGTCGGGGCTCGTCGTCGAAAATGGTGTGCTGGTCGATGAATTTCTGGCG
ACGAGCGATCCCGATATTTATGCCGCCGGGGATATCGCGGCCTGGCCTGATCCGCATAGCGGCCGGCGTATTCGTGTCGA
ACATTGGGATGTCGCTGAAAGACAGGGGCAAATCGCGGCAAAGAACATGCTGGGGCTCGCGACACCCTTCACGGATGTGC
CGTTCTTCTGGACACGCCAGTTCGACTTTTCACTGAGCTATATCGGCCATGCTCCGCATTGGGACAGAATCGAGATCGAC
GGTGATCCATCGGCGGGCGATGGCACTTTGCGCTATATCGACCATGGCCAGGTCATTGCCGCGGCCATGATCGGGCGTGA
CGCGGAATGTCTGCGTGAACGCGAGCGCATGGAACACATTCTCGCGGCGTGA

Upstream 100 bases:

>100_bases
CGGCTTAATGGCCTGCTCCAAATAAACGTGAAGGCGGTCCTCGTCATGCAACGCCGCGCCCATGGCGGCGTTTCCTTCCT
ATCCAATGGGAGGACTATCA

Downstream 100 bases:

>100_bases
ACGTGTATTTCATTTGCGGAAGTTGTGAGGAGGCGGGTGATTACAACGCCTTCTCACGGCGCCTTTCGACAGAGGATGGA
ATTTTGAGGCTGTCGCGATA

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MPSNEQENAAVNTQKTCAKPLFIDLTHPVKLETIPENQIVEACLGEQSAILIREGTTLSILSGKCTHAGGPLAEGLLMEG
HIRCPWHHACFDAHTGEALAAPAFDPLERWAARIEDGMVFVDHKDSTPTHALQAAAPSDPFVIVGGGAAGFAAAEALYHE
GFVGQVVLISREAAGPYDRTMLSKDYLGGKLGEDQLPLHSQAFGQGSGKSAGRIDLGTEVERIDRGRHCVVFADGQELRY
AKLLLATGAEPRAPTFPGYNLPHVHVLRSLADCRSLIEHAAHARHVVVLGASFIGLEAAASLRERGLEVDIVAHSEQPME
KTLGAQLGETLRALHENHGNRFHLGRTILSVEPDRVLLDDGTVLPADLVLVGIGVEPRLDLAKASGLVVENGVLVDEFLA
TSDPDIYAAGDIAAWPDPHSGRRIRVEHWDVAERQGQIAAKNMLGLATPFTDVPFFWTRQFDFSLSYIGHAPHWDRIEID
GDPSAGDGTLRYIDHGQVIAAAMIGRDAECLRERERMEHILAA

Sequences:

>Translated_523_residues
MPSNEQENAAVNTQKTCAKPLFIDLTHPVKLETIPENQIVEACLGEQSAILIREGTTLSILSGKCTHAGGPLAEGLLMEG
HIRCPWHHACFDAHTGEALAAPAFDPLERWAARIEDGMVFVDHKDSTPTHALQAAAPSDPFVIVGGGAAGFAAAEALYHE
GFVGQVVLISREAAGPYDRTMLSKDYLGGKLGEDQLPLHSQAFGQGSGKSAGRIDLGTEVERIDRGRHCVVFADGQELRY
AKLLLATGAEPRAPTFPGYNLPHVHVLRSLADCRSLIEHAAHARHVVVLGASFIGLEAAASLRERGLEVDIVAHSEQPME
KTLGAQLGETLRALHENHGNRFHLGRTILSVEPDRVLLDDGTVLPADLVLVGIGVEPRLDLAKASGLVVENGVLVDEFLA
TSDPDIYAAGDIAAWPDPHSGRRIRVEHWDVAERQGQIAAKNMLGLATPFTDVPFFWTRQFDFSLSYIGHAPHWDRIEID
GDPSAGDGTLRYIDHGQVIAAAMIGRDAECLRERERMEHILAA
>Mature_522_residues
PSNEQENAAVNTQKTCAKPLFIDLTHPVKLETIPENQIVEACLGEQSAILIREGTTLSILSGKCTHAGGPLAEGLLMEGH
IRCPWHHACFDAHTGEALAAPAFDPLERWAARIEDGMVFVDHKDSTPTHALQAAAPSDPFVIVGGGAAGFAAAEALYHEG
FVGQVVLISREAAGPYDRTMLSKDYLGGKLGEDQLPLHSQAFGQGSGKSAGRIDLGTEVERIDRGRHCVVFADGQELRYA
KLLLATGAEPRAPTFPGYNLPHVHVLRSLADCRSLIEHAAHARHVVVLGASFIGLEAAASLRERGLEVDIVAHSEQPMEK
TLGAQLGETLRALHENHGNRFHLGRTILSVEPDRVLLDDGTVLPADLVLVGIGVEPRLDLAKASGLVVENGVLVDEFLAT
SDPDIYAAGDIAAWPDPHSGRRIRVEHWDVAERQGQIAAKNMLGLATPFTDVPFFWTRQFDFSLSYIGHAPHWDRIEIDG
DPSAGDGTLRYIDHGQVIAAAMIGRDAECLRERERMEHILAA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=499, Percent_Identity=31.8637274549098, Blast_Score=253, Evalue=4e-67,
Organism=Homo sapiens, GI21389617, Length=499, Percent_Identity=31.8637274549098, Blast_Score=252, Evalue=5e-67,
Organism=Homo sapiens, GI226437568, Length=499, Percent_Identity=31.8637274549098, Blast_Score=252, Evalue=5e-67,
Organism=Homo sapiens, GI4757732, Length=373, Percent_Identity=27.3458445040214, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI22202629, Length=365, Percent_Identity=28.2191780821918, Blast_Score=93, Evalue=6e-19,
Organism=Escherichia coli, GI1788892, Length=378, Percent_Identity=31.2169312169312, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789765, Length=287, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789065, Length=317, Percent_Identity=26.1829652996845, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17559934, Length=490, Percent_Identity=28.1632653061224, Blast_Score=208, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI32564386, Length=377, Percent_Identity=26.790450928382, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI281359715, Length=522, Percent_Identity=31.2260536398467, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI281359713, Length=522, Percent_Identity=31.2260536398467, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24639250, Length=522, Percent_Identity=31.2260536398467, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI18543267, Length=522, Percent_Identity=31.2260536398467, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24639252, Length=522, Percent_Identity=31.2260536398467, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24639257, Length=522, Percent_Identity=31.0344827586207, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24585130, Length=502, Percent_Identity=30.8764940239044, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24581020, Length=358, Percent_Identity=30.7262569832402, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI28573993, Length=358, Percent_Identity=30.7262569832402, Blast_Score=126, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 56532; Mature: 56400

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSNEQENAAVNTQKTCAKPLFIDLTHPVKLETIPENQIVEACLGEQSAILIREGTTLSI
CCCCCCCCCCCCCHHHHCCCEEEECCCCEEEEECCCHHHHHHHCCCCCEEEEECCCEEEE
LSGKCTHAGGPLAEGLLMEGHIRCPWHHACFDAHTGEALAAPAFDPLERWAARIEDGMVF
ECCCCCCCCCCHHCCEEECCEECCCCHHHHCCCCCCCEEECCCCCHHHHHHHHHHCCEEE
VDHKDSTPTHALQAAAPSDPFVIVGGGAAGFAAAEALYHEGFVGQVVLISREAAGPYDRT
EECCCCCCHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHH
MLSKDYLGGKLGEDQLPLHSQAFGQGSGKSAGRIDLGTEVERIDRGRHCVVFADGQELRY
HHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCEEECCCHHHHHCCCCEEEEEECCCCCEE
AKLLLATGAEPRAPTFPGYNLPHVHVLRSLADCRSLIEHAAHARHVVVLGASFIGLEAAA
EEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHH
SLRERGLEVDIVAHSEQPMEKTLGAQLGETLRALHENHGNRFHLGRTILSVEPDRVLLDD
HHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECEEEEEECCCEEEECC
GTVLPADLVLVGIGVEPRLDLAKASGLVVENGVLVDEFLATSDPDIYAAGDIAAWPDPHS
CCCCCCCEEEEECCCCCCCCHHHCCCEEEECCEEHHHHHHCCCCCEEECCCCCCCCCCCC
GRRIRVEHWDVAERQGQIAAKNMLGLATPFTDVPFFWTRQFDFSLSYIGHAPHWDRIEID
CCEEEEEECCCHHHCCCHHHHHHHHCCCCCCCCCEEEEEEECEEEEEECCCCCCCEEEEC
GDPSAGDGTLRYIDHGQVIAAAMIGRDAECLRERERMEHILAA
CCCCCCCCEEEEEECCCEEEHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PSNEQENAAVNTQKTCAKPLFIDLTHPVKLETIPENQIVEACLGEQSAILIREGTTLSI
CCCCCCCCCCCCHHHHCCCEEEECCCCEEEEECCCHHHHHHHCCCCCEEEEECCCEEEE
LSGKCTHAGGPLAEGLLMEGHIRCPWHHACFDAHTGEALAAPAFDPLERWAARIEDGMVF
ECCCCCCCCCCHHCCEEECCEECCCCHHHHCCCCCCCEEECCCCCHHHHHHHHHHCCEEE
VDHKDSTPTHALQAAAPSDPFVIVGGGAAGFAAAEALYHEGFVGQVVLISREAAGPYDRT
EECCCCCCHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHH
MLSKDYLGGKLGEDQLPLHSQAFGQGSGKSAGRIDLGTEVERIDRGRHCVVFADGQELRY
HHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCEEECCCHHHHHCCCCEEEEEECCCCCEE
AKLLLATGAEPRAPTFPGYNLPHVHVLRSLADCRSLIEHAAHARHVVVLGASFIGLEAAA
EEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHH
SLRERGLEVDIVAHSEQPMEKTLGAQLGETLRALHENHGNRFHLGRTILSVEPDRVLLDD
HHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECEEEEEECCCEEEECC
GTVLPADLVLVGIGVEPRLDLAKASGLVVENGVLVDEFLATSDPDIYAAGDIAAWPDPHS
CCCCCCCEEEEECCCCCCCCHHHCCCEEEECCEEHHHHHHCCCCCEEECCCCCCCCCCCC
GRRIRVEHWDVAERQGQIAAKNMLGLATPFTDVPFFWTRQFDFSLSYIGHAPHWDRIEID
CCEEEEEECCCHHHCCCHHHHHHHHCCCCCCCCCEEEEEEECEEEEEECCCCCCCEEEEC
GDPSAGDGTLRYIDHGQVIAAAMIGRDAECLRERERMEHILAA
CCCCCCCCEEEEEECCCEEEHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]