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The map label for this gene is priA [H]

Identifier: 182677533

GI number: 182677533

Start: 592380

End: 594590

Strand: Direct

Name: priA [H]

Synonym: Bind_0538

Alternate gene names: 182677533

Gene position: 592380-594590 (Clockwise)

Preceding gene: 182677532

Following gene: 182677534

Centisome position: 14.21

GC content: 64.04

Gene sequence:

>2211_bases
ATGAATCGTCTGATCGCTGATGTGTTGATTCCGGTCGCGGTCGATATTGCTTATTCCTATGCGGTCCCCGAGCATCTCGG
CGTCGCGCCGGGCGATTTCGTCGTCGTGCCGCTCGGCAGGCGCCAGGCCGAGGGGATCGTCTGGGACTTGCGCCGGGGCG
CCGCCGACAACCTCAAATTGATTCAGGCACGCCTTGATCTGCCGCCTCTGGCAGAGGATCTGCGCCGCTTCATCGACTGG
GTGGCGCGCTGGACCTTGAGCCCACGCGGCATGGTGTTGCGCATGGCGATCCGCACGCCCTTGCAGGCGCCGCCGGAGCC
GCCGCGCCTCGGAGTCATGCGGGCGGGCGTTTTGCCGGCCCGTATGACACCGGCCCGTCACCGTGTTCTCGAGGCTCTTG
GGGATGGACTCGTTCTCGCCCGAAAGGAACTGGCTAAGAAGGCGGCCTGTTCCCCCAATGTCATCGATGGTCTGATCGAT
GAGGGGACCCTGGCGACGACGGTTTTGGTGGACGAACCGGATAGCGTCCGCGAGGGGATTTCCCATGGTCCCACTCTGGA
ACCGGACCAGGCGGCCGCAGCGGTGGAACTCACCAATCAGGTGCGGCGGCAGACTTTCACTGTCACCCTGCTTGAAGGCG
TGACCGGTTCCGGCAAGACGGAAGTTTATTTCGAGGCCGTCGCCTCGGCCCTCGAAACCGGCGGGCACGCTTTGATCCTC
CTGCCCGAAATCGCGCTCACAGCGCAATTTCTCGATCGTTTTACAACGCGCTTCGGCGAGCGCCCTGGAGAGTGGCATTC
CGCCGTTGGTCCGCGTCGCCGGGCCCGAACCTGGCGGGCCGTGGCCGAGGGGCGGATCAAGGTCGTGGTGGGTGCCCGTT
CAGCCTTGTTTCTCCCCTTCAGGGATTTGCGTCTGATTGTCGTGGATGAGGAACATGATAGCGCCTATAAACAGGAAGAT
GGCGTCCCCTATCATGCCCGCGATATGGCCGTGGTGCGGGGCCAGCTTTCCAAGTCCGCGGTGATCCTGGCCTCGGCGAC
GCCTTCGATCGAGACGCGGGTCAATGCGGAGCAGGGCCGCTATGGGCATCTGCGGCTGCATGCACGCCATGGTGGCCGTG
TTCTGCCTGCTCTTTCCGCCATCGATCTGCGCAAAGCCGGTCCGTCGCCGGGCAAATGGATTTCCCCGCGCCTTGCGCAG
GCGATGACTGAAACCCTTGCGCGAGGCGAGCAGAGTCTGCTTTTTCTCAATCGGCGTGGCTATGCGCCGCTGACGCTTTG
CCGGCTCTGCGGCCATCGTTTCCAATGTCCCCATTGCACGGCATGGCTCGTCGAACATCGGTTCCGCAAGGCGCTCGTTT
GTCATCATTGTGGCCATATTGAACCCGTGCCGGAGGCTTGTCCGGCCTGCGGCGGTCTGCATCATTTGACCGCCTGCGGT
CCCGGAATTGAAAGAGTGGCCGAGGAAGCCGCCGAGCTTTTTCCCAATGCCCGAACCCTTGTTCTCTCCTCGGATCTGCC
CGGCGGCACGGCGCGTATGCGGGACGAACTCGAAGCTGTCGCGCAAGGTGCTTGCGATATTGTCATTGGCACGCAATTGG
TGGCCAAGGGACATAATTTTCCGCTTCTGTCGCTCGTCGGTGTCGTGGATGCGGATATCAGTCTTGCCAATGGCGATCCG
CGCGCTTCCGAGCGGACGTTTCAGATCTTGCGCCAGGTCACCGGGCGCGCCGGCCGTTTCGAGACGCAGGGCCAAGCCCT
GATCCAGACCTGGCAGCCGGAGCATCCGGTCATCGCGGCTCTGCTCTCAGGCGATGCCGAGCGGTTCTATGCCGAGGAAA
CAGAGCAACGCCGGCGTGGCGGCTTGCCGCCCTTTCGTCGGCTGGCCGCTTTGATCATCTCGGCCAAGGAGGCCGCCGTC
GCCGAACGTCACGCCCGCGCTTTGGTCCAGGCGGCTCATGCGATGCCACCCAACCCCGCCTATCTGTTGGCACGAGGGGC
GCCGGGACCGGACGCCGACGGCATCGTCCTATTGGGACCGGCGCCGGCGCCGATCACGGTCATTCGTGCCCGTCATCGCT
TCCGCCTGTTGATCAAGGCGCCGCGTGGCGCCGATCTGCAGGGGTTTATCCGCGCGGTCCTCGCCGCCGCTCCGCCGCCG
CGCGGTGATCTCCGTGTCAGTGTCGATATCGATCCGCAAAGCTTTCTCTGA

Upstream 100 bases:

>100_bases
TGCGCGCCGGCGCAGAAAGCCGCAGGCATGATGTGTTTTGCACGGCCATGCGGGCCGCAGGTGGATAGCGTCGCTCGGCA
ATGACAGGGCCTTCGAACGC

Downstream 100 bases:

>100_bases
CTTTGTTGGATCGGCCAGTTCCTTGCATCCGCAAGCCTGAACCTAGTGAGGCGGCGGCATGACCAAAATGACTTTCATTA
CCTTCGATGGCACCTCCATA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 736; Mature: 736

Protein sequence:

>736_residues
MNRLIADVLIPVAVDIAYSYAVPEHLGVAPGDFVVVPLGRRQAEGIVWDLRRGAADNLKLIQARLDLPPLAEDLRRFIDW
VARWTLSPRGMVLRMAIRTPLQAPPEPPRLGVMRAGVLPARMTPARHRVLEALGDGLVLARKELAKKAACSPNVIDGLID
EGTLATTVLVDEPDSVREGISHGPTLEPDQAAAAVELTNQVRRQTFTVTLLEGVTGSGKTEVYFEAVASALETGGHALIL
LPEIALTAQFLDRFTTRFGERPGEWHSAVGPRRRARTWRAVAEGRIKVVVGARSALFLPFRDLRLIVVDEEHDSAYKQED
GVPYHARDMAVVRGQLSKSAVILASATPSIETRVNAEQGRYGHLRLHARHGGRVLPALSAIDLRKAGPSPGKWISPRLAQ
AMTETLARGEQSLLFLNRRGYAPLTLCRLCGHRFQCPHCTAWLVEHRFRKALVCHHCGHIEPVPEACPACGGLHHLTACG
PGIERVAEEAAELFPNARTLVLSSDLPGGTARMRDELEAVAQGACDIVIGTQLVAKGHNFPLLSLVGVVDADISLANGDP
RASERTFQILRQVTGRAGRFETQGQALIQTWQPEHPVIAALLSGDAERFYAEETEQRRRGGLPPFRRLAALIISAKEAAV
AERHARALVQAAHAMPPNPAYLLARGAPGPDADGIVLLGPAPAPITVIRARHRFRLLIKAPRGADLQGFIRAVLAAAPPP
RGDLRVSVDIDPQSFL

Sequences:

>Translated_736_residues
MNRLIADVLIPVAVDIAYSYAVPEHLGVAPGDFVVVPLGRRQAEGIVWDLRRGAADNLKLIQARLDLPPLAEDLRRFIDW
VARWTLSPRGMVLRMAIRTPLQAPPEPPRLGVMRAGVLPARMTPARHRVLEALGDGLVLARKELAKKAACSPNVIDGLID
EGTLATTVLVDEPDSVREGISHGPTLEPDQAAAAVELTNQVRRQTFTVTLLEGVTGSGKTEVYFEAVASALETGGHALIL
LPEIALTAQFLDRFTTRFGERPGEWHSAVGPRRRARTWRAVAEGRIKVVVGARSALFLPFRDLRLIVVDEEHDSAYKQED
GVPYHARDMAVVRGQLSKSAVILASATPSIETRVNAEQGRYGHLRLHARHGGRVLPALSAIDLRKAGPSPGKWISPRLAQ
AMTETLARGEQSLLFLNRRGYAPLTLCRLCGHRFQCPHCTAWLVEHRFRKALVCHHCGHIEPVPEACPACGGLHHLTACG
PGIERVAEEAAELFPNARTLVLSSDLPGGTARMRDELEAVAQGACDIVIGTQLVAKGHNFPLLSLVGVVDADISLANGDP
RASERTFQILRQVTGRAGRFETQGQALIQTWQPEHPVIAALLSGDAERFYAEETEQRRRGGLPPFRRLAALIISAKEAAV
AERHARALVQAAHAMPPNPAYLLARGAPGPDADGIVLLGPAPAPITVIRARHRFRLLIKAPRGADLQGFIRAVLAAAPPP
RGDLRVSVDIDPQSFL
>Mature_736_residues
MNRLIADVLIPVAVDIAYSYAVPEHLGVAPGDFVVVPLGRRQAEGIVWDLRRGAADNLKLIQARLDLPPLAEDLRRFIDW
VARWTLSPRGMVLRMAIRTPLQAPPEPPRLGVMRAGVLPARMTPARHRVLEALGDGLVLARKELAKKAACSPNVIDGLID
EGTLATTVLVDEPDSVREGISHGPTLEPDQAAAAVELTNQVRRQTFTVTLLEGVTGSGKTEVYFEAVASALETGGHALIL
LPEIALTAQFLDRFTTRFGERPGEWHSAVGPRRRARTWRAVAEGRIKVVVGARSALFLPFRDLRLIVVDEEHDSAYKQED
GVPYHARDMAVVRGQLSKSAVILASATPSIETRVNAEQGRYGHLRLHARHGGRVLPALSAIDLRKAGPSPGKWISPRLAQ
AMTETLARGEQSLLFLNRRGYAPLTLCRLCGHRFQCPHCTAWLVEHRFRKALVCHHCGHIEPVPEACPACGGLHHLTACG
PGIERVAEEAAELFPNARTLVLSSDLPGGTARMRDELEAVAQGACDIVIGTQLVAKGHNFPLLSLVGVVDADISLANGDP
RASERTFQILRQVTGRAGRFETQGQALIQTWQPEHPVIAALLSGDAERFYAEETEQRRRGGLPPFRRLAALIISAKEAAV
AERHARALVQAAHAMPPNPAYLLARGAPGPDADGIVLLGPAPAPITVIRARHRFRLLIKAPRGADLQGFIRAVLAAAPPP
RGDLRVSVDIDPQSFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=754, Percent_Identity=33.289124668435, Blast_Score=351, Evalue=1e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 79861; Mature: 79861

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLIADVLIPVAVDIAYSYAVPEHLGVAPGDFVVVPLGRRQAEGIVWDLRRGAADNLKL
CCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEECCCCCCCCEEEEHHCCCCCCHHH
IQARLDLPPLAEDLRRFIDWVARWTLSPRGMVLRMAIRTPLQAPPEPPRLGVMRAGVLPA
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCCCCCCCCCCCCHHHCCCCCC
RMTPARHRVLEALGDGLVLARKELAKKAACSPNVIDGLIDEGTLATTVLVDEPDSVREGI
CCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEEECCCHHHHHHH
SHGPTLEPDQAAAAVELTNQVRRQTFTVTLLEGVTGSGKTEVYFEAVASALETGGHALIL
CCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEE
LPEIALTAQFLDRFTTRFGERPGEWHSAVGPRRRARTWRAVAEGRIKVVVGARSALFLPF
ECHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHCCCEEEEEECCCEEEEEE
RDLRLIVVDEEHDSAYKQEDGVPYHARDMAVVRGQLSKSAVILASATPSIETRVNAEQGR
CCCEEEEEECCCCCHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCC
YGHLRLHARHGGRVLPALSAIDLRKAGPSPGKWISPRLAQAMTETLARGEQSLLFLNRRG
EEEEEEEECCCCEECHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCC
YAPLTLCRLCGHRFQCPHCTAWLVEHRFRKALVCHHCGHIEPVPEACPACGGLHHLTACG
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHCC
PGIERVAEEAAELFPNARTLVLSSDLPGGTARMRDELEAVAQGACDIVIGTQLVAKGHNF
CHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHCCCCC
PLLSLVGVVDADISLANGDPRASERTFQILRQVTGRAGRFETQGQALIQTWQPEHPVIAA
CHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCEEHH
LLSGDAERFYAEETEQRRRGGLPPFRRLAALIISAKEAAVAERHARALVQAAHAMPPNPA
HHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YLLARGAPGPDADGIVLLGPAPAPITVIRARHRFRLLIKAPRGADLQGFIRAVLAAAPPP
EEEEECCCCCCCCCEEEECCCCCCCHHEECCCEEEEEEECCCCCCHHHHHHHHHHCCCCC
RGDLRVSVDIDPQSFL
CCCEEEEEECCCHHCC
>Mature Secondary Structure
MNRLIADVLIPVAVDIAYSYAVPEHLGVAPGDFVVVPLGRRQAEGIVWDLRRGAADNLKL
CCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEECCCCCCCCEEEEHHCCCCCCHHH
IQARLDLPPLAEDLRRFIDWVARWTLSPRGMVLRMAIRTPLQAPPEPPRLGVMRAGVLPA
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCCCCCCCCCCCCHHHCCCCCC
RMTPARHRVLEALGDGLVLARKELAKKAACSPNVIDGLIDEGTLATTVLVDEPDSVREGI
CCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEEECCCHHHHHHH
SHGPTLEPDQAAAAVELTNQVRRQTFTVTLLEGVTGSGKTEVYFEAVASALETGGHALIL
CCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEE
LPEIALTAQFLDRFTTRFGERPGEWHSAVGPRRRARTWRAVAEGRIKVVVGARSALFLPF
ECHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHCCCEEEEEECCCEEEEEE
RDLRLIVVDEEHDSAYKQEDGVPYHARDMAVVRGQLSKSAVILASATPSIETRVNAEQGR
CCCEEEEEECCCCCHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCC
YGHLRLHARHGGRVLPALSAIDLRKAGPSPGKWISPRLAQAMTETLARGEQSLLFLNRRG
EEEEEEEECCCCEECHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCC
YAPLTLCRLCGHRFQCPHCTAWLVEHRFRKALVCHHCGHIEPVPEACPACGGLHHLTACG
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHCC
PGIERVAEEAAELFPNARTLVLSSDLPGGTARMRDELEAVAQGACDIVIGTQLVAKGHNF
CHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHCCCCC
PLLSLVGVVDADISLANGDPRASERTFQILRQVTGRAGRFETQGQALIQTWQPEHPVIAA
CHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCEEHH
LLSGDAERFYAEETEQRRRGGLPPFRRLAALIISAKEAAVAERHARALVQAAHAMPPNPA
HHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YLLARGAPGPDADGIVLLGPAPAPITVIRARHRFRLLIKAPRGADLQGFIRAVLAAAPPP
EEEEECCCCCCCCCEEEECCCCCCCHHEECCCEEEEEEECCCCCCHHHHHHHHHHCCCCC
RGDLRVSVDIDPQSFL
CCCEEEEEECCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]