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The map label for this gene is nifV [H]

Identifier: 182677481

GI number: 182677481

Start: 544916

End: 546118

Strand: Direct

Name: nifV [H]

Synonym: Bind_0485

Alternate gene names: 182677481

Gene position: 544916-546118 (Clockwise)

Preceding gene: 182677480

Following gene: 182677482

Centisome position: 13.07

GC content: 56.61

Gene sequence:

>1203_bases
ATGGTGGCCTCTATGACGCGTTCCGTAATGGATCTGGCGAAGACGCCTATCATTCTTAACGATACGACTTTGCGGGACGG
AGAGCAGGCGCCAGGCGTCGCTTTTTCTCCCGAAGAAAAAATAGCGATCGCGCGGGCCTTGGCGCTGGCCGGGGTTCCTG
AAATTGAAGCCGGCACGCCGGCCATGGGGGAGCGGGAAATCGCCGCGATCAATGCGGTTGTCGCTGAGGGGCTCCCTTCG
AAAATCATTGCCTGGTGCAGGATGGTGCCGCAGGATATTGATGCGGCGGTGGCATCCGGTGTCGGCATGATCAATCTTTC
ATTGCCCGTGTCCGATATTCAGCTCAAGGCCAAATTTGGCGCTGGTCGAGCCTATGCGCGCGAACTCATCGGCCGTTTCA
TTCCCTATGCGCGTGACAAGGGTTTGGAAGTGGCTCTCGGATGCGAGGATGCTTCACGCGCTGACGTAGATTTTCTCCTA
GAAGTGGCAGAACTTGGTGCGTCCCTGGGTGTCAGGCGTATGCGCTTTGCCGATACACTCGGTGTGCTGGATCCTTTCAT
GACTTTTGCCATGATCGAGCGATTGCGGCAGGCGAGTGATCTGGAAATTGAAATTCATGCGCATGATGATCTCGGCTTGG
CGACCGCCAATACTTTGGCGGCATTGAGAGCCGGTGCCACCCATGCGAGCGTCACCGTGGTGGGACTCGGCGAAAGAGCG
GGCAATGCGCCACTGGAGGAAGTCGCCGTTGCCGTTGAAGCGCTTTATGGTTTTACGACGGGCGTCGATTTGACGGCTTT
GGCGCAGGTCGCGCAGGTTGTGACAAATGCCGCGGGCCGTTCTATTCCGGAGGCCAAAGCTATCGTGGGTGAAGCCGTCT
TCACACATGAATCCGGAATTCACGTCGACGGGTTGCTCAAGGATTTACACTGTTATCAAGCCTTGGATCCGGCGCTTTTG
GGGCGTTCGCACCACGTGGTTCTTGGGAAACATTCCGGCTTGCGCGCGGTGACGAATGCCCTGGCTGAACAAGGTCTCAG
CGTTTCAGCGGACGAGGCTAAATTCGTGCTGGCACGAATCAAGCTCTATGCGGAGCAGGAAAAAGCTTCTGTTCCAGGCG
ACATTTTGCTCGATTTCTATCGTGAAGCGAAAGTCGCGAGCGGGCATGCGGTCGATGCCTGGACCTCGCTTGTTATCAAT
TGA

Upstream 100 bases:

>100_bases
TCAGCCCTAATCTGCCCAAGCTGTAATTGTCTTCGCTCGGATCGTGAGAATGGGCTTCATACCTTGGGTGTGAGGCTCTT
TTTCGGGCCGAGGTGGTTTG

Downstream 100 bases:

>100_bases
ATGTGATTTTCGCTTTTACCGGGCAGAGAATCCAGGCGAAGACGGGCGCTATCGGCCGTTTCGTTTGAGGTTGGCCCGTT
GCGGAAATTTGAGCGGCCGG

Product: homocitrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MVASMTRSVMDLAKTPIILNDTTLRDGEQAPGVAFSPEEKIAIARALALAGVPEIEAGTPAMGEREIAAINAVVAEGLPS
KIIAWCRMVPQDIDAAVASGVGMINLSLPVSDIQLKAKFGAGRAYARELIGRFIPYARDKGLEVALGCEDASRADVDFLL
EVAELGASLGVRRMRFADTLGVLDPFMTFAMIERLRQASDLEIEIHAHDDLGLATANTLAALRAGATHASVTVVGLGERA
GNAPLEEVAVAVEALYGFTTGVDLTALAQVAQVVTNAAGRSIPEAKAIVGEAVFTHESGIHVDGLLKDLHCYQALDPALL
GRSHHVVLGKHSGLRAVTNALAEQGLSVSADEAKFVLARIKLYAEQEKASVPGDILLDFYREAKVASGHAVDAWTSLVIN

Sequences:

>Translated_400_residues
MVASMTRSVMDLAKTPIILNDTTLRDGEQAPGVAFSPEEKIAIARALALAGVPEIEAGTPAMGEREIAAINAVVAEGLPS
KIIAWCRMVPQDIDAAVASGVGMINLSLPVSDIQLKAKFGAGRAYARELIGRFIPYARDKGLEVALGCEDASRADVDFLL
EVAELGASLGVRRMRFADTLGVLDPFMTFAMIERLRQASDLEIEIHAHDDLGLATANTLAALRAGATHASVTVVGLGERA
GNAPLEEVAVAVEALYGFTTGVDLTALAQVAQVVTNAAGRSIPEAKAIVGEAVFTHESGIHVDGLLKDLHCYQALDPALL
GRSHHVVLGKHSGLRAVTNALAEQGLSVSADEAKFVLARIKLYAEQEKASVPGDILLDFYREAKVASGHAVDAWTSLVIN
>Mature_400_residues
MVASMTRSVMDLAKTPIILNDTTLRDGEQAPGVAFSPEEKIAIARALALAGVPEIEAGTPAMGEREIAAINAVVAEGLPS
KIIAWCRMVPQDIDAAVASGVGMINLSLPVSDIQLKAKFGAGRAYARELIGRFIPYARDKGLEVALGCEDASRADVDFLL
EVAELGASLGVRRMRFADTLGVLDPFMTFAMIERLRQASDLEIEIHAHDDLGLATANTLAALRAGATHASVTVVGLGERA
GNAPLEEVAVAVEALYGFTTGVDLTALAQVAQVVTNAAGRSIPEAKAIVGEAVFTHESGIHVDGLLKDLHCYQALDPALL
GRSHHVVLGKHSGLRAVTNALAEQGLSVSADEAKFVLARIKLYAEQEKASVPGDILLDFYREAKVASGHAVDAWTSLVIN

Specific function: This protein is a Fe-Mo-cofactor biosynthetic component [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=348, Percent_Identity=33.6206896551724, Blast_Score=169, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6320071, Length=367, Percent_Identity=30.5177111716621, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6320019, Length=344, Percent_Identity=31.3953488372093, Blast_Score=167, Evalue=3e-42,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.14 [H]

Molecular weight: Translated: 42097; Mature: 42097

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVASMTRSVMDLAKTPIILNDTTLRDGEQAPGVAFSPEEKIAIARALALAGVPEIEAGTP
CCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
AMGEREIAAINAVVAEGLPSKIIAWCRMVPQDIDAAVASGVGMINLSLPVSDIQLKAKFG
CCCCHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCEEEEEECC
AGRAYARELIGRFIPYARDKGLEVALGCEDASRADVDFLLEVAELGASLGVRRMRFADTL
CCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHH
GVLDPFMTFAMIERLRQASDLEIEIHAHDDLGLATANTLAALRAGATHASVTVVGLGERA
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCC
GNAPLEEVAVAVEALYGFTTGVDLTALAQVAQVVTNAAGRSIPEAKAIVGEAVFTHESGI
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCC
HVDGLLKDLHCYQALDPALLGRSHHVVLGKHSGLRAVTNALAEQGLSVSADEAKFVLARI
CHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
KLYAEQEKASVPGDILLDFYREAKVASGHAVDAWTSLVIN
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MVASMTRSVMDLAKTPIILNDTTLRDGEQAPGVAFSPEEKIAIARALALAGVPEIEAGTP
CCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
AMGEREIAAINAVVAEGLPSKIIAWCRMVPQDIDAAVASGVGMINLSLPVSDIQLKAKFG
CCCCHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCEEEEEECC
AGRAYARELIGRFIPYARDKGLEVALGCEDASRADVDFLLEVAELGASLGVRRMRFADTL
CCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHH
GVLDPFMTFAMIERLRQASDLEIEIHAHDDLGLATANTLAALRAGATHASVTVVGLGERA
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCC
GNAPLEEVAVAVEALYGFTTGVDLTALAQVAQVVTNAAGRSIPEAKAIVGEAVFTHESGI
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCC
HVDGLLKDLHCYQALDPALLGRSHHVVLGKHSGLRAVTNALAEQGLSVSADEAKFVLARI
CHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
KLYAEQEKASVPGDILLDFYREAKVASGHAVDAWTSLVIN
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644218; 3040672 [H]