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The map label for this gene is purA

Identifier: 182677261

GI number: 182677261

Start: 301068

End: 302363

Strand: Direct

Name: purA

Synonym: Bind_0262

Alternate gene names: 182677261

Gene position: 301068-302363 (Clockwise)

Preceding gene: 182677258

Following gene: 182677263

Centisome position: 7.22

GC content: 60.73

Gene sequence:

>1296_bases
ATGGCGAATGTGGTGGTAGTCGGCGCCCAATGGGGCGACGAGGGCAAGGGCAAGATCGTTGACTGGCTCTCGATCGAGGC
GGACATCGTCGTCCGCTTCCAGGGCGGGCACAATGCCGGTCATACCCTGGTCATCGGCAATCAGGTTTATAAGCTTGCGC
TCCTGCCATCGGGCATCGTGCGGCTGGGCAAATTATCCGTCATTGGCAATGGCGTCGTCGTTGATCCGCAGCATCTGGTG
GAAGAAATTGCCAAATTGGCGGCGCAGGGCATCGAGATCACGCCGGACAATCTGAAAATCGCCGAAAACGTACCTCTGAT
TCTCGGCCTGCATCGCGAACTCGACGCGCATCGCGAATCGTCCACGGTCGAGGGTGTGAAGATCGGCACGACCAAGCGTG
GCATCGGTCCCGCTTATGAAGACAAGGTCGGACGCCGCGCCATCCGCCTCATGGATCTTGCCGAGCCGGATACGCTTGAT
GAAAAGATCGTGCGCCTTCTCGCCCATCACGAGCCCTTGCGGCGCGGTCTCGGCCTCGAGCCGATAAGCGCTGGGGCGAT
CCGCGCCGAGCTCGAAAGCCTAGCTCCCAAAATCCTGCCCTTCATGGATGCGACCTGGGATCTGCTTGAGAATGCGCGCC
GAGCCGGCAAGCGCATTCTGTTCGAGGGGGCGCAAGGGGCCTTGCTCGATATCGATCACGGCACCTATCCTTTCGTGACC
TCCTCCAATACGGTGGCGGCCAATGCGGCGACGGGATCGGGCCTCGGCCCCAAGGCGATCGGTTATGTGCTGGGCATCGC
CAAGGCTTATACGACGCGTGTCGGTGGCGGCCCCTTTCCGACCGAATTGCTCGACGAAACGGGTCAATTGATCGGTGATC
GCGGTCACGAATATGGCGTCAATACCGGCCGGCGGCGGCGCTGCGGCTGGTTCGATGCGGTTTTGGTGCGCCAGACCGTC
AAGACCTGCGGTATCGATGGAATCGCCTTGACCAAGCTCGATATTCTGGATGGTTTCAAGGAGATCAAGGTTTGTGTCGG
CTATGATCTCGACGGCCGTCGCATCGATCGTCTGCCGGCAAGCCAATCGGCTCAGGCTCGTGTGAAGCCGATCTATGAAA
CGATCGACGGTTGGGAAGGCACAACGGCCGGAGCCCGCTCCTGGGCCGATCTGCCGGCCCAGGCGATCAAATATGTGCGG
CGGATCGAGGAATTGATCGAAGCGCCGGTGGCGCTCTTGTCGACGAGCCCGGAACGCGCCGATACAATCCTCGTCCATGA
TCCGTTCCGGGATTGA

Upstream 100 bases:

>100_bases
TGCATACTCGGGATGAAAGCCGGGCGCGTCCTTTCGCGCCGGCCGATCGCAAAATCATTTTCCTGACACCTTAATAAAGA
CTCGGAAGCGAACGCGTCGC

Downstream 100 bases:

>100_bases
CGTCATCTTGATGCAAGCAAACGGGACCAGTCTGGTATCGAGAATCACAGATTCTCGATCCGTCACTTTTTAAACTTATC
CACTTGTCCCCGTTGATCCG

Product: adenylosuccinate synthase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MANVVVVGAQWGDEGKGKIVDWLSIEADIVVRFQGGHNAGHTLVIGNQVYKLALLPSGIVRLGKLSVIGNGVVVDPQHLV
EEIAKLAAQGIEITPDNLKIAENVPLILGLHRELDAHRESSTVEGVKIGTTKRGIGPAYEDKVGRRAIRLMDLAEPDTLD
EKIVRLLAHHEPLRRGLGLEPISAGAIRAELESLAPKILPFMDATWDLLENARRAGKRILFEGAQGALLDIDHGTYPFVT
SSNTVAANAATGSGLGPKAIGYVLGIAKAYTTRVGGGPFPTELLDETGQLIGDRGHEYGVNTGRRRRCGWFDAVLVRQTV
KTCGIDGIALTKLDILDGFKEIKVCVGYDLDGRRIDRLPASQSAQARVKPIYETIDGWEGTTAGARSWADLPAQAIKYVR
RIEELIEAPVALLSTSPERADTILVHDPFRD

Sequences:

>Translated_431_residues
MANVVVVGAQWGDEGKGKIVDWLSIEADIVVRFQGGHNAGHTLVIGNQVYKLALLPSGIVRLGKLSVIGNGVVVDPQHLV
EEIAKLAAQGIEITPDNLKIAENVPLILGLHRELDAHRESSTVEGVKIGTTKRGIGPAYEDKVGRRAIRLMDLAEPDTLD
EKIVRLLAHHEPLRRGLGLEPISAGAIRAELESLAPKILPFMDATWDLLENARRAGKRILFEGAQGALLDIDHGTYPFVT
SSNTVAANAATGSGLGPKAIGYVLGIAKAYTTRVGGGPFPTELLDETGQLIGDRGHEYGVNTGRRRRCGWFDAVLVRQTV
KTCGIDGIALTKLDILDGFKEIKVCVGYDLDGRRIDRLPASQSAQARVKPIYETIDGWEGTTAGARSWADLPAQAIKYVR
RIEELIEAPVALLSTSPERADTILVHDPFRD
>Mature_430_residues
ANVVVVGAQWGDEGKGKIVDWLSIEADIVVRFQGGHNAGHTLVIGNQVYKLALLPSGIVRLGKLSVIGNGVVVDPQHLVE
EIAKLAAQGIEITPDNLKIAENVPLILGLHRELDAHRESSTVEGVKIGTTKRGIGPAYEDKVGRRAIRLMDLAEPDTLDE
KIVRLLAHHEPLRRGLGLEPISAGAIRAELESLAPKILPFMDATWDLLENARRAGKRILFEGAQGALLDIDHGTYPFVTS
SNTVAANAATGSGLGPKAIGYVLGIAKAYTTRVGGGPFPTELLDETGQLIGDRGHEYGVNTGRRRRCGWFDAVLVRQTVK
TCGIDGIALTKLDILDGFKEIKVCVGYDLDGRRIDRLPASQSAQARVKPIYETIDGWEGTTAGARSWADLPAQAIKYVRR
IEELIEAPVALLSTSPERADTILVHDPFRD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=424, Percent_Identity=44.3396226415094, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=43.9252336448598, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=42.3857868020305, Blast_Score=298, Evalue=5e-81,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=50.4651162790698, Blast_Score=419, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI25146553, Length=427, Percent_Identity=43.3255269320843, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI25146555, Length=427, Percent_Identity=43.3255269320843, Blast_Score=333, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=39.3939393939394, Blast_Score=310, Evalue=3e-85,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=43.1603773584906, Blast_Score=328, Evalue=4e-90,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_BEII9 (B2ICM9)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001831407.1
- GeneID:   6199228
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_0262
- HOGENOM:   HBG658237
- OMA:   ERDDTIV
- ProtClustDB:   CLSK2613283
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46543; Mature: 46412

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 131-131 BINDING 145-145 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVVGAQWGDEGKGKIVDWLSIEADIVVRFQGGHNAGHTLVIGNQVYKLALLPSGIV
CCCEEEEECCCCCCCCCCEEEEEEECEEEEEEEECCCCCCCEEEECCCEEEEEECCHHHH
RLGKLSVIGNGVVVDPQHLVEEIAKLAAQGIEITPDNLKIAENVPLILGLHRELDAHRES
HHCEEEEECCCEEECHHHHHHHHHHHHHCCCEECCCCEEECCCCCEEEEEHHHHHHHCCC
STVEGVKIGTTKRGIGPAYEDKVGRRAIRLMDLAEPDTLDEKIVRLLAHHEPLRRGLGLE
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHCCCCC
PISAGAIRAELESLAPKILPFMDATWDLLENARRAGKRILFEGAQGALLDIDHGTYPFVT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCCEEE
SSNTVAANAATGSGLGPKAIGYVLGIAKAYTTRVGGGPFPTELLDETGQLIGDRGHEYGV
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCC
NTGRRRRCGWFDAVLVRQTVKTCGIDGIALTKLDILDGFKEIKVCVGYDLDGRRIDRLPA
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHCCHHHEEEEEECCCCCCCCCCCCC
SQSAQARVKPIYETIDGWEGTTAGARSWADLPAQAIKYVRRIEELIEAPVALLSTSPERA
CCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCC
DTILVHDPFRD
CEEEEECCCCC
>Mature Secondary Structure 
ANVVVVGAQWGDEGKGKIVDWLSIEADIVVRFQGGHNAGHTLVIGNQVYKLALLPSGIV
CCEEEEECCCCCCCCCCEEEEEEECEEEEEEEECCCCCCCEEEECCCEEEEEECCHHHH
RLGKLSVIGNGVVVDPQHLVEEIAKLAAQGIEITPDNLKIAENVPLILGLHRELDAHRES
HHCEEEEECCCEEECHHHHHHHHHHHHHCCCEECCCCEEECCCCCEEEEEHHHHHHHCCC
STVEGVKIGTTKRGIGPAYEDKVGRRAIRLMDLAEPDTLDEKIVRLLAHHEPLRRGLGLE
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHCCCCC
PISAGAIRAELESLAPKILPFMDATWDLLENARRAGKRILFEGAQGALLDIDHGTYPFVT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCCEEE
SSNTVAANAATGSGLGPKAIGYVLGIAKAYTTRVGGGPFPTELLDETGQLIGDRGHEYGV
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCC
NTGRRRRCGWFDAVLVRQTVKTCGIDGIALTKLDILDGFKEIKVCVGYDLDGRRIDRLPA
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHCCHHHEEEEEECCCCCCCCCCCCC
SQSAQARVKPIYETIDGWEGTTAGARSWADLPAQAIKYVRRIEELIEAPVALLSTSPERA
CCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCC
DTILVHDPFRD
CEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA