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The map label for this gene is hslU

Identifier: 182677250

GI number: 182677250

Start: 288011

End: 289318

Strand: Direct

Name: hslU

Synonym: Bind_0251

Alternate gene names: 182677250

Gene position: 288011-289318 (Clockwise)

Preceding gene: 182677249

Following gene: 182677256

Centisome position: 6.91

GC content: 58.49

Gene sequence:

>1308_bases
ATGACCGATTTTTCACCCCGTGAAATCGTTTCCGAGCTGGACCGCTATATCGTCGGGCAGAACGATGCCAAGCGTGCCGT
GGCCATCGCGCTGCGCAACCGCTGGCGCCGGCTTAAGCTTGAAGGCGCGATGCGCGAGGAGGTTTTGCCGAAAAACATCC
TGATGATCGGCCCGACCGGTTGCGGCAAGACCGAGATCGCCCGACGCCTCGCCAAGCTCGCCAATGCGCCTTTCCTGAAG
GTCGAGGCGACGAAATTCACCGAAGTCGGTTATGTCGGACGCGATGTCGAGCAGATCATTCGCGATCTGGTCGAGACGGC
GATCGTCATGGTCAAGGCGGAAAAGCGCAAGTCGCTCGAAGCAAAGGCGCATCAGTCGGTCGAGGAACGCCTGCTCGATG
CCCTGGTCGGCGCCAATGCCGCGCAGACCACGCGCGATTCCTTTCGCAAGAAACTTCGCGACGGAGACCTGGAGGATAAG
GAAATCGAGATCGAACTGGCGCAGGCTACGGGCAATCTGCCGATGTTCGATCTGCCAAACATGCCGGGTGCGGCGGTCGG
GGCGATCTCCATCGGCGATATTTTCGCGAAATCACTGGGCCGTTCCACCAAGCGCCGGCGCAGCACCGTCAAGGAGGCGC
GCGAACCGCTCCTGGCCGAGGAAAGCGAAAAGCTGATGGATCAGGAACAGATCATCGCGCAGGCCATTCATTCCGTTGAG
AATAATGGCATCGTCTTTCTCGATGAAATCGACAAGATTTGCGCGCGGGAAGGACGTGGCGGCGCGGATGTTTCACGCGA
AGGCGTGCAGCGCGATCTTCTTCCCCTGATCGAAGGGACCAGCGTCGCCACCAAACATGGGACGATCAAGACCGATCATA
TTCTCTTCATCGCCTCGGGCGCCTTTCATGTCGCCAAGCCTTCCGATCTCTTGCCGGAATTGCAGGGCAGACTGCCGATC
CGCGTCGAGCTGCTGCCCCTCAATGAAGAGGATTTCCGCCGAATCCTGACTGAAACGGAAGTCAGTCTTATCAAGCAATA
TGAGGCTTTGCTCGCGACCGAGGGGGTGAACTTGGTCTTTACGCCGGAGGCGGTGAGCAGTCTGGCAAAGGTCGCCGTGC
AGGTGAATTCCAGTGTCGAAAATATTGGCGCGCGGCGTTTGCAGACGGTGATGGAGCGCGTGCTCGATGAGGTAAGTTAT
ACAGCGCCGGACCGATCCGGACAGACGATCACGATCGATGCCGCCTATGTCGAGAAGAATATCGGCGATCTCGCCAAAAA
TACGGATCTGAGCCGGTTCATCCTGTGA

Upstream 100 bases:

>100_bases
TTTGCGTCTACACCAATCGCAATCTCATTATCGAATCGATTGATGCCTGAGCCTGCAAGGGTTTGGGCTTTCTTCTCCTC
ATTTTTTACGCGATGCCTTC

Downstream 100 bases:

>100_bases
GGTCACGGCGAGAGCATCTGACCCAAAAGTGGCTCCCCCTTTTTTGGATCAATCTGATGCGTTTTCAAAAAAGAGAGAGC
ATCGGATAGGAGTCCGGTGC

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTDFSPREIVSELDRYIVGQNDAKRAVAIALRNRWRRLKLEGAMREEVLPKNILMIGPTGCGKTEIARRLAKLANAPFLK
VEATKFTEVGYVGRDVEQIIRDLVETAIVMVKAEKRKSLEAKAHQSVEERLLDALVGANAAQTTRDSFRKKLRDGDLEDK
EIEIELAQATGNLPMFDLPNMPGAAVGAISIGDIFAKSLGRSTKRRRSTVKEAREPLLAEESEKLMDQEQIIAQAIHSVE
NNGIVFLDEIDKICAREGRGGADVSREGVQRDLLPLIEGTSVATKHGTIKTDHILFIASGAFHVAKPSDLLPELQGRLPI
RVELLPLNEEDFRRILTETEVSLIKQYEALLATEGVNLVFTPEAVSSLAKVAVQVNSSVENIGARRLQTVMERVLDEVSY
TAPDRSGQTITIDAAYVEKNIGDLAKNTDLSRFIL

Sequences:

>Translated_435_residues
MTDFSPREIVSELDRYIVGQNDAKRAVAIALRNRWRRLKLEGAMREEVLPKNILMIGPTGCGKTEIARRLAKLANAPFLK
VEATKFTEVGYVGRDVEQIIRDLVETAIVMVKAEKRKSLEAKAHQSVEERLLDALVGANAAQTTRDSFRKKLRDGDLEDK
EIEIELAQATGNLPMFDLPNMPGAAVGAISIGDIFAKSLGRSTKRRRSTVKEAREPLLAEESEKLMDQEQIIAQAIHSVE
NNGIVFLDEIDKICAREGRGGADVSREGVQRDLLPLIEGTSVATKHGTIKTDHILFIASGAFHVAKPSDLLPELQGRLPI
RVELLPLNEEDFRRILTETEVSLIKQYEALLATEGVNLVFTPEAVSSLAKVAVQVNSSVENIGARRLQTVMERVLDEVSY
TAPDRSGQTITIDAAYVEKNIGDLAKNTDLSRFIL
>Mature_434_residues
TDFSPREIVSELDRYIVGQNDAKRAVAIALRNRWRRLKLEGAMREEVLPKNILMIGPTGCGKTEIARRLAKLANAPFLKV
EATKFTEVGYVGRDVEQIIRDLVETAIVMVKAEKRKSLEAKAHQSVEERLLDALVGANAAQTTRDSFRKKLRDGDLEDKE
IEIELAQATGNLPMFDLPNMPGAAVGAISIGDIFAKSLGRSTKRRRSTVKEAREPLLAEESEKLMDQEQIIAQAIHSVEN
NGIVFLDEIDKICAREGRGGADVSREGVQRDLLPLIEGTSVATKHGTIKTDHILFIASGAFHVAKPSDLLPELQGRLPIR
VELLPLNEEDFRRILTETEVSLIKQYEALLATEGVNLVFTPEAVSSLAKVAVQVNSSVENIGARRLQTVMERVLDEVSYT
APDRSGQTITIDAAYVEKNIGDLAKNTDLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Escherichia coli, GI1790366, Length=447, Percent_Identity=57.9418344519016, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1786642, Length=113, Percent_Identity=43.3628318584071, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319704, Length=123, Percent_Identity=36.5853658536585, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648291, Length=264, Percent_Identity=29.5454545454545, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24648289, Length=264, Percent_Identity=29.5454545454545, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_BEII9 (B2ICL8)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001831396.1
- GeneID:   6198307
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_0251
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- ProtClustDB:   PRK05201
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 48084; Mature: 47953

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 248-248 BINDING 313-313 BINDING 385-385

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFSPREIVSELDRYIVGQNDAKRAVAIALRNRWRRLKLEGAMREEVLPKNILMIGPTG
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
CGKTEIARRLAKLANAPFLKVEATKFTEVGYVGRDVEQIIRDLVETAIVMVKAEKRKSLE
CCHHHHHHHHHHHHCCCEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAHQSVEERLLDALVGANAAQTTRDSFRKKLRDGDLEDKEIEIELAQATGNLPMFDLPN
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCC
MPGAAVGAISIGDIFAKSLGRSTKRRRSTVKEAREPLLAEESEKLMDQEQIIAQAIHSVE
CCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
NNGIVFLDEIDKICAREGRGGADVSREGVQRDLLPLIEGTSVATKHGTIKTDHILFIASG
CCCEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCEEECEEEEEECC
AFHVAKPSDLLPELQGRLPIRVELLPLNEEDFRRILTETEVSLIKQYEALLATEGVNLVF
CEECCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
TPEAVSSLAKVAVQVNSSVENIGARRLQTVMERVLDEVSYTAPDRSGQTITIDAAYVEKN
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHH
IGDLAKNTDLSRFIL
HHHHHHCCCHHHHCC
>Mature Secondary Structure 
TDFSPREIVSELDRYIVGQNDAKRAVAIALRNRWRRLKLEGAMREEVLPKNILMIGPTG
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
CGKTEIARRLAKLANAPFLKVEATKFTEVGYVGRDVEQIIRDLVETAIVMVKAEKRKSLE
CCHHHHHHHHHHHHCCCEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAHQSVEERLLDALVGANAAQTTRDSFRKKLRDGDLEDKEIEIELAQATGNLPMFDLPN
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCC
MPGAAVGAISIGDIFAKSLGRSTKRRRSTVKEAREPLLAEESEKLMDQEQIIAQAIHSVE
CCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
NNGIVFLDEIDKICAREGRGGADVSREGVQRDLLPLIEGTSVATKHGTIKTDHILFIASG
CCCEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCEEECEEEEEECC
AFHVAKPSDLLPELQGRLPIRVELLPLNEEDFRRILTETEVSLIKQYEALLATEGVNLVF
CEECCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
TPEAVSSLAKVAVQVNSSVENIGARRLQTVMERVLDEVSYTAPDRSGQTITIDAAYVEKN
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHH
IGDLAKNTDLSRFIL
HHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA