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The map label for this gene is ruvB

Identifier: 182677134

GI number: 182677134

Start: 156383

End: 157435

Strand: Direct

Name: ruvB

Synonym: Bind_0133

Alternate gene names: 182677134

Gene position: 156383-157435 (Clockwise)

Preceding gene: 182677133

Following gene: 182677137

Centisome position: 3.75

GC content: 61.54

Gene sequence:

>1053_bases
TTGAGCAAAACGCCTGAACGCCTCGTGACCTCCGAGGTCCGCGACGAAGATATGACGGAAGCCTCGCTGCGTCCGCTCAC
CCTCGCTGATTTTACCGGTCAGGCGGCGGCGCGCCAGAATCTGAGTGTCTTCATTGCGGCCGCCAAGGCCCGCCGGGAAG
CGCTGGATCATGTGCTGTTCGTTGGCCCGCCGGGGCTTGGCAAAACCACGCTGGCGCAAATCGTCGCGCGGGAGCTCGGC
GTCAATTTCCGCTCAACCTCAGGTCCGGTGATCGCCAAGGCGGGCGATCTCGCCGCGCAACTGACGGCGTTGGAAGAACG
CGATGTCCTGTTCATCGACGAGATTCACCGCCTCAATCCGGCGGTCGAGGAAATTCTCTATCCGGCCATGGAGGATTTCC
AGCTCGATCTCATCATCGGCGAGGGACCGGGCGCGCGCTCGGTCAAGATCGATCTTGCCCGTTTCACCTTGGTTGGTGCG
ACGACCCGGGCGGGCCTTCTCACGACGCCGCTCCGTGATCGTTTCGGCATTCCGATCCGGCTCGAATTCTATACGATCGA
GGAATTGGAGCGGATCGTCCTGCGCGGGGCGCGGGTGCAGGGGCTGGCGCTTGAAAAGGAAGGCGCCAATGAGATTGCCA
AAAGAGCCCGAGGCACACCACGCATCGCGGGCCGGCTTTTGCGCCGCGTGCGTGATTTCGCCATTGTCGATGGTGTCGAA
AGCGTGACGCGCACCCTCGCCGACAAGGCGCTTTCCCTGCTTGATGTGGATCCGATCGGTCTCGACCAGATGGATCGCCG
TTATCTTAATGTGATTGCGCTCTCCTTTGGCGGTGGTCCGGTCGGAATCGAAACCATCGCCGCCGCTCTGTCCGAGCCGC
GCGACGCGATCGAGGATATTATCGAGCCCTATCTGATCCAACAGGGCTTTCTACAACGCACGCCGCGCGGACGTTTATTG
ACGCCGCATGCCTTTCGTCATCTGGGCCTCAAGGAACCGGTGCGCGAGACGCCGCAATATGGCCTGTTCCCTGATCAGTC
GGAAGAGGATTGA

Upstream 100 bases:

>100_bases
CCGCAAACCAGGCCTTTATTCCATGCTCGACGTTTTGGGGCTTGGTGGGGAATAATGGAACCGCCGATTCATCGGCTTGC
TTTATTCACGAGACCCCGCC

Downstream 100 bases:

>100_bases
GCCCGGCCTGAGAGGCGTTTTTCAGGGTCGTTGAACCGGCTCCAGGAGAAGAGGGTTGTTTTTATCCTGTATTCGCTCTG
ATGGCGGAGCATAGGGGAGC

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSKTPERLVTSEVRDEDMTEASLRPLTLADFTGQAAARQNLSVFIAAAKARREALDHVLFVGPPGLGKTTLAQIVARELG
VNFRSTSGPVIAKAGDLAAQLTALEERDVLFIDEIHRLNPAVEEILYPAMEDFQLDLIIGEGPGARSVKIDLARFTLVGA
TTRAGLLTTPLRDRFGIPIRLEFYTIEELERIVLRGARVQGLALEKEGANEIAKRARGTPRIAGRLLRRVRDFAIVDGVE
SVTRTLADKALSLLDVDPIGLDQMDRRYLNVIALSFGGGPVGIETIAAALSEPRDAIEDIIEPYLIQQGFLQRTPRGRLL
TPHAFRHLGLKEPVRETPQYGLFPDQSEED

Sequences:

>Translated_350_residues
MSKTPERLVTSEVRDEDMTEASLRPLTLADFTGQAAARQNLSVFIAAAKARREALDHVLFVGPPGLGKTTLAQIVARELG
VNFRSTSGPVIAKAGDLAAQLTALEERDVLFIDEIHRLNPAVEEILYPAMEDFQLDLIIGEGPGARSVKIDLARFTLVGA
TTRAGLLTTPLRDRFGIPIRLEFYTIEELERIVLRGARVQGLALEKEGANEIAKRARGTPRIAGRLLRRVRDFAIVDGVE
SVTRTLADKALSLLDVDPIGLDQMDRRYLNVIALSFGGGPVGIETIAAALSEPRDAIEDIIEPYLIQQGFLQRTPRGRLL
TPHAFRHLGLKEPVRETPQYGLFPDQSEED
>Mature_349_residues
SKTPERLVTSEVRDEDMTEASLRPLTLADFTGQAAARQNLSVFIAAAKARREALDHVLFVGPPGLGKTTLAQIVARELGV
NFRSTSGPVIAKAGDLAAQLTALEERDVLFIDEIHRLNPAVEEILYPAMEDFQLDLIIGEGPGARSVKIDLARFTLVGAT
TRAGLLTTPLRDRFGIPIRLEFYTIEELERIVLRGARVQGLALEKEGANEIAKRARGTPRIAGRLLRRVRDFAIVDGVES
VTRTLADKALSLLDVDPIGLDQMDRRYLNVIALSFGGGPVGIETIAAALSEPRDAIEDIIEPYLIQQGFLQRTPRGRLLT
PHAFRHLGLKEPVRETPQYGLFPDQSEED

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=318, Percent_Identity=61.6352201257862, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1787119, Length=234, Percent_Identity=28.6324786324786, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_BEII9 (B2IBR9)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001831280.1
- GeneID:   6199432
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_0133
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR000641
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00819
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 38472; Mature: 38341

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTPERLVTSEVRDEDMTEASLRPLTLADFTGQAAARQNLSVFIAAAKARREALDHVLF
CCCCHHHHHHHHHCCCHHHHHHCCCEEEECCCCCHHHHCCCEEEEEHHHHHHHHHHCEEE
VGPPGLGKTTLAQIVARELGVNFRSTSGPVIAKAGDLAAQLTALEERDVLFIDEIHRLNP
ECCCCCCHHHHHHHHHHHHCCCEECCCCCEEEECCHHHHHHHHHCCCCEEEHHHHHHCCH
AVEEILYPAMEDFQLDLIIGEGPGARSVKIDLARFTLVGATTRAGLLTTPLRDRFGIPIR
HHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEEHEEEEECCCCCCEEECCHHHHCCCCEE
LEFYTIEELERIVLRGARVQGLALEKEGANEIAKRARGTPRIAGRLLRRVRDFAIVDGVE
EEEEEHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
SVTRTLADKALSLLDVDPIGLDQMDRRYLNVIALSFGGGPVGIETIAAALSEPRDAIEDI
HHHHHHHHHHHHHHCCCCCCCCHHHHHHEEEEEEECCCCCCCHHHHHHHHHCHHHHHHHH
IEPYLIQQGFLQRTPRGRLLTPHAFRHLGLKEPVRETPQYGLFPDQSEED
HHHHHHHHHHHHCCCCCCEECHHHHHHCCCCCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKTPERLVTSEVRDEDMTEASLRPLTLADFTGQAAARQNLSVFIAAAKARREALDHVLF
CCCHHHHHHHHHCCCHHHHHHCCCEEEECCCCCHHHHCCCEEEEEHHHHHHHHHHCEEE
VGPPGLGKTTLAQIVARELGVNFRSTSGPVIAKAGDLAAQLTALEERDVLFIDEIHRLNP
ECCCCCCHHHHHHHHHHHHCCCEECCCCCEEEECCHHHHHHHHHCCCCEEEHHHHHHCCH
AVEEILYPAMEDFQLDLIIGEGPGARSVKIDLARFTLVGATTRAGLLTTPLRDRFGIPIR
HHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEEHEEEEECCCCCCEEECCHHHHCCCCEE
LEFYTIEELERIVLRGARVQGLALEKEGANEIAKRARGTPRIAGRLLRRVRDFAIVDGVE
EEEEEHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
SVTRTLADKALSLLDVDPIGLDQMDRRYLNVIALSFGGGPVGIETIAAALSEPRDAIEDI
HHHHHHHHHHHHHHCCCCCCCCHHHHHHEEEEEEECCCCCCCHHHHHHHHHCHHHHHHHH
IEPYLIQQGFLQRTPRGRLLTPHAFRHLGLKEPVRETPQYGLFPDQSEED
HHHHHHHHHHHHCCCCCCEECHHHHHHCCCCCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA