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The map label for this gene is pyrF [H]

Identifier: 182677132

GI number: 182677132

Start: 154730

End: 155485

Strand: Direct

Name: pyrF [H]

Synonym: Bind_0131

Alternate gene names: 182677132

Gene position: 154730-155485 (Clockwise)

Preceding gene: 182677131

Following gene: 182677133

Centisome position: 3.71

GC content: 63.36

Gene sequence:

>756_bases
ATGAACGCACCCTCATCCTCTCCCGATAAGCCGCTGGATCCACGCGACCGGCTTATCGTCGCTCTCGATGTGACCGATCC
GGCGGCTGCGCGGACCTTGGTGAATCGGCTGGGCGACAGCGTGTCTTTCTACAAGATCGGTATGGAACTCGCCTATGGCG
GCGGTCTGCCGTTCGCGAGCGAGCTGCTCAAGGAAGGCAAGAAGGTTTTTCTCGACCTCAAGCTGCATGATATCGCGACG
ACCGTCAGCAACGCGGTGGCGCAGGTTGCGCGGCTCGGCGTGCATTTTCTGACGGTTCATGCTTATCCGCAAACGCTTGA
TGCCGCGCGGCGTGGTGCCGTTGGCTCGCCCTTGAAAATCCTCGGCGTCAGTGTGCTCACCTCTTATGATGACGCCGATC
TCGCCGAGGCGGGCTATGCCTACTCTGTTGCCGATCTCGTGGCGCGCCGGGCGCTGCAGGCGCGCGAGGCGGGCGTCGAT
GGGCTGGTTCTCTCGGCGGCGGAAGCAGCCGCGATCAGGTCCCAGCTTGGTTCTGATCTGATCCTGGTGACGCCCGGCAT
CCGGCCGCGCGGCACCTCCGCCAAGGATCAAAAGCGTGTGGCGACACCCTCACTCGCCATTGCCGCTGGCGCTGATCATC
TCGTCGTCGGACGGCCGATTACGGTGGCTCCCGATCCAAAAGGCGCCGCTGATCAGATCGTCGCGGAAATCGAGGCGACG
CTTGCATCCATGGCTCCCCCGCCGGATAAGCCATGA

Upstream 100 bases:

>100_bases
TGCATTTCGGTGATAAGGCCAAAGCCGTGCCGCTCAAGCGGTCCATGGAGCCCTTGCGGGGGCAGCGCACGGATGGCTCG
CGCGAACGCGGACGGCGCTC

Downstream 100 bases:

>100_bases
CGAGAGTCTGGCTGAAGTAGATCGGAGAGTTCTCCCATGAGCGAGTTGAAGCTCGTTGTCGCGGGCGCCGCCGGGCGCAT
GGGCCGCGTGCTCATCCAGA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNAPSSSPDKPLDPRDRLIVALDVTDPAAARTLVNRLGDSVSFYKIGMELAYGGGLPFASELLKEGKKVFLDLKLHDIAT
TVSNAVAQVARLGVHFLTVHAYPQTLDAARRGAVGSPLKILGVSVLTSYDDADLAEAGYAYSVADLVARRALQAREAGVD
GLVLSAAEAAAIRSQLGSDLILVTPGIRPRGTSAKDQKRVATPSLAIAAGADHLVVGRPITVAPDPKGAADQIVAEIEAT
LASMAPPPDKP

Sequences:

>Translated_251_residues
MNAPSSSPDKPLDPRDRLIVALDVTDPAAARTLVNRLGDSVSFYKIGMELAYGGGLPFASELLKEGKKVFLDLKLHDIAT
TVSNAVAQVARLGVHFLTVHAYPQTLDAARRGAVGSPLKILGVSVLTSYDDADLAEAGYAYSVADLVARRALQAREAGVD
GLVLSAAEAAAIRSQLGSDLILVTPGIRPRGTSAKDQKRVATPSLAIAAGADHLVVGRPITVAPDPKGAADQIVAEIEAT
LASMAPPPDKP
>Mature_251_residues
MNAPSSSPDKPLDPRDRLIVALDVTDPAAARTLVNRLGDSVSFYKIGMELAYGGGLPFASELLKEGKKVFLDLKLHDIAT
TVSNAVAQVARLGVHFLTVHAYPQTLDAARRGAVGSPLKILGVSVLTSYDDADLAEAGYAYSVADLVARRALQAREAGVD
GLVLSAAEAAAIRSQLGSDLILVTPGIRPRGTSAKDQKRVATPSLAIAAGADHLVVGRPITVAPDPKGAADQIVAEIEAT
LASMAPPPDKP

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=227, Percent_Identity=39.2070484581498, Blast_Score=140, Evalue=7e-35,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 26044; Mature: 26044

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPSSSPDKPLDPRDRLIVALDVTDPAAARTLVNRLGDSVSFYKIGMELAYGGGLPFAS
CCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECEEEEECCCCHHHH
ELLKEGKKVFLDLKLHDIATTVSNAVAQVARLGVHFLTVHAYPQTLDAARRGAVGSPLKI
HHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCCCHHH
LGVSVLTSYDDADLAEAGYAYSVADLVARRALQAREAGVDGLVLSAAEAAAIRSQLGSDL
HHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCE
ILVTPGIRPRGTSAKDQKRVATPSLAIAAGADHLVVGRPITVAPDPKGAADQIVAEIEAT
EEECCCCCCCCCCCCHHHHHCCCCEEEECCCCEEEECCCEEECCCCCCHHHHHHHHHHHH
LASMAPPPDKP
HHHCCCCCCCC
>Mature Secondary Structure
MNAPSSSPDKPLDPRDRLIVALDVTDPAAARTLVNRLGDSVSFYKIGMELAYGGGLPFAS
CCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECEEEEECCCCHHHH
ELLKEGKKVFLDLKLHDIATTVSNAVAQVARLGVHFLTVHAYPQTLDAARRGAVGSPLKI
HHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCCCHHH
LGVSVLTSYDDADLAEAGYAYSVADLVARRALQAREAGVDGLVLSAAEAAAIRSQLGSDL
HHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCE
ILVTPGIRPRGTSAKDQKRVATPSLAIAAGADHLVVGRPITVAPDPKGAADQIVAEIEAT
EEECCCCCCCCCCCCHHHHHCCCCEEEECCCCEEEECCCEEECCCCCCHHHHHHHHHHHH
LASMAPPPDKP
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA