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The map label for this gene is gcvA [H]

Identifier: 17547480

GI number: 17547480

Start: 2973271

End: 2974197

Strand: Direct

Name: gcvA [H]

Synonym: RSc2761

Alternate gene names: 17547480

Gene position: 2973271-2974197 (Clockwise)

Preceding gene: 17547466

Following gene: 17547481

Centisome position: 80.0

GC content: 69.26

Gene sequence:

>927_bases
ATGCCGAGACGCCTCCCTCCCCTCAATGCATTGCGCGTCTTTGAAGTCGCCGGCCGCCATCTGAGCTTCAGCCGCGCCGC
CGACGAGCTATGCGTGACCAACGCCGCGGTCAGCCACCAGATCAAGCAGCTCGAAGACCACCTCGGCCTGAAGCTGTTCC
GCCGCCGCAACAACCAGCTCACCCTGACCGATGCCGGCGACAACTACCTGCCGCGCGTGCGCGACGCCCTGCGCGCGCTG
GAACAGGCCACCGACCTGCTGATGGGCACCGGCGATGCCCCGCTGCGCGTGGCCGTGCCGCCGACCTTCGGCGCCAAATG
GCTGGTGCCGCGCCTGTACCGCTTCTTCAACCAGCATCCGCAGGTGCGCGTCGAGGTCTCCACCGCCGACGTGCAGGACC
ACGGCCAGTTCGATCTCTGCATCGACGACCGCCAGGTCAGCGTGCCCGGCCTGCGCGTCGAGTGGTTCACCGCCACCGAT
TTCTTCCCGGTGTGCAGCGCCGCTCTGCAGCCGGCCATCCGCTTACCGCAGGACCTTGCCACGCAGACGCTGCTGCACGA
GCGCGGCGGACGCCATCTCGCCCACCATCCGACCTGGCAGCAATGGCTCGACGAAGTGGGCGTGCGGCAGATCGATGCTG
CGCGTGGCCCCGCGTTCTCCGAAGCGCTGATGGCCATGCAGGCGGCCATCGACGGCCAGGGCGTGGCGCTCGGCCAGGGC
CTGCTGGTGGAATACGACATCGCCGCCGGGCGGCTGGTGCGGCCGCTGGCGACCGAGGCGTCGCTGCGCCTGTCGTACTA
CCTGATTCACCCCCACCAGGCCTCCGAGAACCCCGGCTTCGCGCTGTTCCGGCAATGGCTGATCGACGAACTTGCGCGCG
GCGGCGGACGCGGACGCCATCCGCAGACCCCGGTCGAGGTGTTTTGA

Upstream 100 bases:

>100_bases
CGACCGCGCCGTCCCCAGGTGCGATTTGTAGTCGGTGCGGCGAGAACGTGGCAAACTACAAATACTTCGCGGTACGTCAA
ATTTTTCTTAACGAAAAATC

Downstream 100 bases:

>100_bases
TTTCTTTTTGATTGTGGCGGTGCGTGCGCTCTAAACTAGCTGTCCGGAGTCCCGCCCAGCCGCAGGAGCAGACCATGAGT
TTCCCCCGTCCCGCCAACAC

Product: transcription regulator protein

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MPRRLPPLNALRVFEVAGRHLSFSRAADELCVTNAAVSHQIKQLEDHLGLKLFRRRNNQLTLTDAGDNYLPRVRDALRAL
EQATDLLMGTGDAPLRVAVPPTFGAKWLVPRLYRFFNQHPQVRVEVSTADVQDHGQFDLCIDDRQVSVPGLRVEWFTATD
FFPVCSAALQPAIRLPQDLATQTLLHERGGRHLAHHPTWQQWLDEVGVRQIDAARGPAFSEALMAMQAAIDGQGVALGQG
LLVEYDIAAGRLVRPLATEASLRLSYYLIHPHQASENPGFALFRQWLIDELARGGGRGRHPQTPVEVF

Sequences:

>Translated_308_residues
MPRRLPPLNALRVFEVAGRHLSFSRAADELCVTNAAVSHQIKQLEDHLGLKLFRRRNNQLTLTDAGDNYLPRVRDALRAL
EQATDLLMGTGDAPLRVAVPPTFGAKWLVPRLYRFFNQHPQVRVEVSTADVQDHGQFDLCIDDRQVSVPGLRVEWFTATD
FFPVCSAALQPAIRLPQDLATQTLLHERGGRHLAHHPTWQQWLDEVGVRQIDAARGPAFSEALMAMQAAIDGQGVALGQG
LLVEYDIAAGRLVRPLATEASLRLSYYLIHPHQASENPGFALFRQWLIDELARGGGRGRHPQTPVEVF
>Mature_307_residues
PRRLPPLNALRVFEVAGRHLSFSRAADELCVTNAAVSHQIKQLEDHLGLKLFRRRNNQLTLTDAGDNYLPRVRDALRALE
QATDLLMGTGDAPLRVAVPPTFGAKWLVPRLYRFFNQHPQVRVEVSTADVQDHGQFDLCIDDRQVSVPGLRVEWFTATDF
FPVCSAALQPAIRLPQDLATQTLLHERGGRHLAHHPTWQQWLDEVGVRQIDAARGPAFSEALMAMQAAIDGQGVALGQGL
LVEYDIAAGRLVRPLATEASLRLSYYLIHPHQASENPGFALFRQWLIDELARGGGRGRHPQTPVEVF

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=303, Percent_Identity=42.9042904290429, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1786448, Length=302, Percent_Identity=32.7814569536424, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=29.1095890410959, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI145693193, Length=289, Percent_Identity=26.643598615917, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI157672245, Length=180, Percent_Identity=32.7777777777778, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787589, Length=308, Percent_Identity=23.7012987012987, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1786401, Length=113, Percent_Identity=33.6283185840708, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787128, Length=126, Percent_Identity=30.1587301587302, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87081978, Length=311, Percent_Identity=27.0096463022508, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34387; Mature: 34255

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRLPPLNALRVFEVAGRHLSFSRAADELCVTNAAVSHQIKQLEDHLGLKLFRRRNNQL
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
TLTDAGDNYLPRVRDALRALEQATDLLMGTGDAPLRVAVPPTFGAKWLVPRLYRFFNQHP
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCC
QVRVEVSTADVQDHGQFDLCIDDRQVSVPGLRVEWFTATDFFPVCSAALQPAIRLPQDLA
CEEEEEEECCCCCCCCEEEEECCCEECCCCEEEEEEECCCCHHHHHHHHHHHHCCCHHHH
TQTLLHERGGRHLAHHPTWQQWLDEVGVRQIDAARGPAFSEALMAMQAAIDGQGVALGQG
HHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCCCCEEECCC
LLVEYDIAAGRLVRPLATEASLRLSYYLIHPHQASENPGFALFRQWLIDELARGGGRGRH
EEEEEECCCCHHHHHHHHCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
PQTPVEVF
CCCCCCCC
>Mature Secondary Structure 
PRRLPPLNALRVFEVAGRHLSFSRAADELCVTNAAVSHQIKQLEDHLGLKLFRRRNNQL
CCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
TLTDAGDNYLPRVRDALRALEQATDLLMGTGDAPLRVAVPPTFGAKWLVPRLYRFFNQHP
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCC
QVRVEVSTADVQDHGQFDLCIDDRQVSVPGLRVEWFTATDFFPVCSAALQPAIRLPQDLA
CEEEEEEECCCCCCCCEEEEECCCEECCCCEEEEEEECCCCHHHHHHHHHHHHCCCHHHH
TQTLLHERGGRHLAHHPTWQQWLDEVGVRQIDAARGPAFSEALMAMQAAIDGQGVALGQG
HHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCCCCEEECCC
LLVEYDIAAGRLVRPLATEASLRLSYYLIHPHQASENPGFALFRQWLIDELARGGGRGRH
EEEEEECCCCHHHHHHHHCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
PQTPVEVF
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]