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The map label for this gene is gudD1 [H]

Identifier: 17545798

GI number: 17545798

Start: 1134803

End: 1136164

Strand: Direct

Name: gudD1 [H]

Synonym: RSc1079

Alternate gene names: 17545798

Gene position: 1134803-1136164 (Clockwise)

Preceding gene: 17545796

Following gene: 17545799

Centisome position: 30.53

GC content: 69.75

Gene sequence:

>1362_bases
GTGACGATCATGAATCACAACTCCGTGGAATATGCTGTCCGCACGCCGCGCATCACGCGCCTCCAGGTCATTCCGGTGGC
GGGGCGCGACAGCATGCTGCTCAACCTGAGCGGCGCGCACGCGCCGTTCTTCACCCGCAACATCGCCATCCTGGAAGATT
CCGACGGCCATCTCGGCGTGGGCGAAGTGCCGGGCGGCGAGGCGATCCGCAAGACCATCGAGGATGCGGCGCCGCTGGTG
GTCGGCCAGTCGATCGGCGCCTACAACAACGTGCTGAACACGGTGCGCCAGCGCTTCGCCGACCGCGATGCCACCGGGCG
CGGCACGCAGACGTTCGACCTGCGCACCACCGTGCACGCGGTGACCGCGCTGGAGAGCGCGCTGCTTGACCTGCTCGGCC
AGCATCTGGGCGTGCCGGTGGCGGCCTTGCTGGGGCAGGGCCAGCAGCGTGCCACGGTGCCGGTGCTCGGCTACCTGTTC
TTCGTGGGCGATCGCACCCGCACCGACCTGGACTACGCCGATGGCAGCGACGCGACCGACGACTGGACGCACCTGCGCCA
CCAGGAGGCGCTGACGCCGGAGGCGGTGGTGAAGCTGGCGCGGGCGGCCCATGCGCGCTACGGCTTCAAGGATTTCAAGC
TCAAGGGCGGCGTGCTCAGCGGCGACGATGAGATGACGGTGACGACCGCGCTGGCCGAAGCCTTCCCCGAGGCGCGCGTC
ACGCTCGACCCGAACGGTGCCTGGTCGCTGGCCGAGGCGGTCCGCCTGTGCCGCGACAAGCACGGCGTGCTGGCCTATGC
CGAAGACCCGTGCGGCGCGGAGAACGGCTATTCCGGACGCGAGGTCATGGCCGAATTCCGCCGCGCCACCGGGCTGCCCA
CCGCGACCAACATGATCGCCACCGACTGGCGCCAGATGTGCCACGCGGTCCAGCTGCACGCCGTGGATATCCCGCTGGCC
GACCCGCATTTCTGGACCCTGCAGGGCTCGGTGCGCGTGGCGCAGATGTGTGCCGAGTGGGGCCTGACTTGGGGCTCGCA
CTCCAACAACCATTTCGACATTTCGCTGGCCATGTTCACGCACGTGGCCGCGGCGGCGCCGGGCAACATCACCGCCATCG
ACACGCATTGGATCTGGCAGGACGGCCAGCGCCTGACGTGCGAGCCGTTGCGCATCGCCGGCGGCGAGATCGCCGTGCCG
ACCGCGCCGGGGCTGGGCGTCACGCTCGACATGGCGCAGCTGGAGAAGGCCCATGCGCTGTACAAGCAGCACGGGCTGGG
CGCGCGCGACGATGCCGCCGCGATGCGCTGCCTGGTGCCGGGCTGGCAGTTCGACAACAAGCGGCCGTGCCTGGTGCGCT
GA

Upstream 100 bases:

>100_bases
TGCTGTTTCCGGTGGGGTGTTCGGGGTTTACCAGGGGTGTACCTGCAAGGACGTGTCCATTATAGTCCGATCGCATGTTG
TACGACGACTGATAACATAC

Downstream 100 bases:

>100_bases
GGCGCGGCTAGGCCATCCGGGGGGCGCTGCGCCGCTCCCCTCGGTTTCAACCCATAAAAAAGACATCCGGAGACAACGCG
TGAAGACCATCAAGGGCCTG

Product: glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTIMNHNSVEYAVRTPRITRLQVIPVAGRDSMLLNLSGAHAPFFTRNIAILEDSDGHLGVGEVPGGEAIRKTIEDAAPLV
VGQSIGAYNNVLNTVRQRFADRDATGRGTQTFDLRTTVHAVTALESALLDLLGQHLGVPVAALLGQGQQRATVPVLGYLF
FVGDRTRTDLDYADGSDATDDWTHLRHQEALTPEAVVKLARAAHARYGFKDFKLKGGVLSGDDEMTVTTALAEAFPEARV
TLDPNGAWSLAEAVRLCRDKHGVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIATDWRQMCHAVQLHAVDIPLA
DPHFWTLQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHVAAAAPGNITAIDTHWIWQDGQRLTCEPLRIAGGEIAVP
TAPGLGVTLDMAQLEKAHALYKQHGLGARDDAAAMRCLVPGWQFDNKRPCLVR

Sequences:

>Translated_453_residues
MTIMNHNSVEYAVRTPRITRLQVIPVAGRDSMLLNLSGAHAPFFTRNIAILEDSDGHLGVGEVPGGEAIRKTIEDAAPLV
VGQSIGAYNNVLNTVRQRFADRDATGRGTQTFDLRTTVHAVTALESALLDLLGQHLGVPVAALLGQGQQRATVPVLGYLF
FVGDRTRTDLDYADGSDATDDWTHLRHQEALTPEAVVKLARAAHARYGFKDFKLKGGVLSGDDEMTVTTALAEAFPEARV
TLDPNGAWSLAEAVRLCRDKHGVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIATDWRQMCHAVQLHAVDIPLA
DPHFWTLQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHVAAAAPGNITAIDTHWIWQDGQRLTCEPLRIAGGEIAVP
TAPGLGVTLDMAQLEKAHALYKQHGLGARDDAAAMRCLVPGWQFDNKRPCLVR
>Mature_452_residues
TIMNHNSVEYAVRTPRITRLQVIPVAGRDSMLLNLSGAHAPFFTRNIAILEDSDGHLGVGEVPGGEAIRKTIEDAAPLVV
GQSIGAYNNVLNTVRQRFADRDATGRGTQTFDLRTTVHAVTALESALLDLLGQHLGVPVAALLGQGQQRATVPVLGYLFF
VGDRTRTDLDYADGSDATDDWTHLRHQEALTPEAVVKLARAAHARYGFKDFKLKGGVLSGDDEMTVTTALAEAFPEARVT
LDPNGAWSLAEAVRLCRDKHGVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIATDWRQMCHAVQLHAVDIPLAD
PHFWTLQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHVAAAAPGNITAIDTHWIWQDGQRLTCEPLRIAGGEIAVPT
APGLGVTLDMAQLEKAHALYKQHGLGARDDAAAMRCLVPGWQFDNKRPCLVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc). Also acts on L-idarate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=440, Percent_Identity=72.0454545454545, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=442, Percent_Identity=63.3484162895928, Blast_Score=553, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 49162; Mature: 49031

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIMNHNSVEYAVRTPRITRLQVIPVAGRDSMLLNLSGAHAPFFTRNIAILEDSDGHLGV
CEECCCCCEEEEEECCCEEEEEEEEECCCCCEEEEECCCCCCEEECEEEEEECCCCCEEC
GEVPGGEAIRKTIEDAAPLVVGQSIGAYNNVLNTVRQRFADRDATGRGTQTFDLRTTVHA
CCCCCHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHH
VTALESALLDLLGQHLGVPVAALLGQGQQRATVPVLGYLFFVGDRTRTDLDYADGSDATD
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHEEEEHEECCCCCCCCCCCCCCCCCC
DWTHLRHQEALTPEAVVKLARAAHARYGFKDFKLKGGVLSGDDEMTVTTALAEAFPEARV
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCEECCCCCCHHHHHHHHHCCCCEE
TLDPNGAWSLAEAVRLCRDKHGVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIA
EECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHH
TDWRQMCHAVQLHAVDIPLADPHFWTLQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFT
HHHHHHHHHHHEEEEECCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHH
HVAAAAPGNITAIDTHWIWQDGQRLTCEPLRIAGGEIAVPTAPGLGVTLDMAQLEKAHAL
HHHHCCCCCEEEEEEEEEECCCCEEEECCEEECCCEEEECCCCCCCEEEEHHHHHHHHHH
YKQHGLGARDDAAAMRCLVPGWQFDNKRPCLVR
HHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TIMNHNSVEYAVRTPRITRLQVIPVAGRDSMLLNLSGAHAPFFTRNIAILEDSDGHLGV
EECCCCCEEEEEECCCEEEEEEEEECCCCCEEEEECCCCCCEEECEEEEEECCCCCEEC
GEVPGGEAIRKTIEDAAPLVVGQSIGAYNNVLNTVRQRFADRDATGRGTQTFDLRTTVHA
CCCCCHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHH
VTALESALLDLLGQHLGVPVAALLGQGQQRATVPVLGYLFFVGDRTRTDLDYADGSDATD
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHEEEEHEECCCCCCCCCCCCCCCCCC
DWTHLRHQEALTPEAVVKLARAAHARYGFKDFKLKGGVLSGDDEMTVTTALAEAFPEARV
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCEECCCCCCHHHHHHHHHCCCCEE
TLDPNGAWSLAEAVRLCRDKHGVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIA
EECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHH
TDWRQMCHAVQLHAVDIPLADPHFWTLQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFT
HHHHHHHHHHHEEEEECCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHH
HVAAAAPGNITAIDTHWIWQDGQRLTCEPLRIAGGEIAVPTAPGLGVTLDMAQLEKAHAL
HHHHCCCCCEEEEEEEEEECCCCEEEECCEEECCCEEEECCCCCCCEEEEHHHHHHHHHH
YKQHGLGARDDAAAMRCLVPGWQFDNKRPCLVR
HHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]