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The map label for this gene is gdhA [H]

Identifier: 17545199

GI number: 17545199

Start: 515594

End: 516895

Strand: Direct

Name: gdhA [H]

Synonym: RSc0480

Alternate gene names: 17545199

Gene position: 515594-516895 (Clockwise)

Preceding gene: 17545198

Following gene: 17545200

Centisome position: 13.87

GC content: 65.51

Gene sequence:

>1302_bases
ATGTCCAATGTCGCATCTCCGTCGACGCTCCAGCACGCCATTCCGTCCTACCTGCCAGCCGATAACCTCGGTCCGTGGGG
CATCTACCTGCAGCAGGTCGATCGGGTCACCCCCTATCTCGGCTCGCTGGCACGCTGGGTCGAAACCCTGAAGCGTCCCA
AGCGCGCCCTGATCGTCGACGTGCCCATCCAGATGGATGACGGCCGCATCGCCCACTTCGAGGGCTACCGCGTGCAGCAC
AACACATCGCGCGGCCCGGGCAAGGGCGGGGTGCGTTTCCACCAGGACGTGACGCTGTCGGAAGTGATGGCGCTGTCGGC
CTGGATGTCGGTCAAGAACGCGGCCGTCAACGTGCCGTACGGCGGCGCCAAGGGCGGCGTCCGCGTCGATCCGCGCAAGC
TGTCGTCGGGCGAGCTCGAGCGCCTGACCCGCCGCTACACCAGCGAGATCGGCATCATCATCGGCCCGAACAAGGACATC
CCGGCGCCGGACGTGAACACCAACGCGCAGATCATGGCGTGGATGATGGACACGTACTCCATGAACGAAGGCGCCACCGC
CACCGGCGTGGTGACCGGCAAGCCGATCGCGCTGGGCGGCAGCCTGGGCCGCCGCGAGGCGACCGGCCGCGGCGTGTTCG
TGGTCGGCAGCGAGGCTGCACGCAATCTGGGCATCGACGTCAAGGGTGCGCGCATCGTGGTGCAAGGCTTCGGCAACGTC
GGCAGCGTGGCCGCCAAGCTGTTCCAGGATGCCGGCGCCAAGGTGATCGCGGTGCAGGACCACAAGGGCATCGTGTTCAA
CGGCGCGGGCCTGGACGTCGACGCGCTGATCCAGCACGTGGACCACAACGGCAGCGTCGACGGCTTCAAGGCCGAGACCC
TGTCGGCGGATGATTTCTGGGCGCTGGAATGCGAATTCCTGATCCCGGCCGCGCTCGAAGGCCAGATCACCGGCAAGAAC
GCGCCCCAAATCAAGGCAAAAATTGTCGTTGAAGGTGCAAACGGCCCCACGACGCCCGAAGCGGACGACATCCTGCGCGA
TCGCGGCATCCTGGTCTGCCCGGACGTGATCGCCAATGCCGGCGGCGTCACGGTGAGCTATTTCGAGTGGGTGCAGGATT
TCTCGAGCTTCTTCTGGACCGAAGACGAGATCAACCAGCGCCTGGTACGGATCATGCAAGATGCTTTCCGCGCCATCTGG
CAGGTGGCCCAGGACAACAAGGTAACTCTGCGGACGGCGGCGTTCATCATCGCCTGTACGCGGATTCTGCAGGCGCGCCA
AGAGCGCGGTCTGTACCCCTGA

Upstream 100 bases:

>100_bases
AAACAGCATTGGATTTCGTTCAGGGGCCTGCCTAAGCTGGCGGGCATTTTACAGGCACTCACGAGCCTTACCCAGGCTCC
AAAGGAAGAGGAAGACAGCC

Downstream 100 bases:

>100_bases
TCGGCGACGCGTCGGTCAATACCGGCGCGGTGCCTCATCAGGGTATCGCTCCGGTCTATCGCGTCGCCGCCGATCAGGTG
GCCATGCGCGACGGTTGAAG

Product: glutamate dehydrogenase (NAD(P)+) oxidoreductase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSNVASPSTLQHAIPSYLPADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRALIVDVPIQMDDGRIAHFEGYRVQH
NTSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGVRVDPRKLSSGELERLTRRYTSEIGIIIGPNKDI
PAPDVNTNAQIMAWMMDTYSMNEGATATGVVTGKPIALGGSLGRREATGRGVFVVGSEAARNLGIDVKGARIVVQGFGNV
GSVAAKLFQDAGAKVIAVQDHKGIVFNGAGLDVDALIQHVDHNGSVDGFKAETLSADDFWALECEFLIPAALEGQITGKN
APQIKAKIVVEGANGPTTPEADDILRDRGILVCPDVIANAGGVTVSYFEWVQDFSSFFWTEDEINQRLVRIMQDAFRAIW
QVAQDNKVTLRTAAFIIACTRILQARQERGLYP

Sequences:

>Translated_433_residues
MSNVASPSTLQHAIPSYLPADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRALIVDVPIQMDDGRIAHFEGYRVQH
NTSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGVRVDPRKLSSGELERLTRRYTSEIGIIIGPNKDI
PAPDVNTNAQIMAWMMDTYSMNEGATATGVVTGKPIALGGSLGRREATGRGVFVVGSEAARNLGIDVKGARIVVQGFGNV
GSVAAKLFQDAGAKVIAVQDHKGIVFNGAGLDVDALIQHVDHNGSVDGFKAETLSADDFWALECEFLIPAALEGQITGKN
APQIKAKIVVEGANGPTTPEADDILRDRGILVCPDVIANAGGVTVSYFEWVQDFSSFFWTEDEINQRLVRIMQDAFRAIW
QVAQDNKVTLRTAAFIIACTRILQARQERGLYP
>Mature_432_residues
SNVASPSTLQHAIPSYLPADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRALIVDVPIQMDDGRIAHFEGYRVQHN
TSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGVRVDPRKLSSGELERLTRRYTSEIGIIIGPNKDIP
APDVNTNAQIMAWMMDTYSMNEGATATGVVTGKPIALGGSLGRREATGRGVFVVGSEAARNLGIDVKGARIVVQGFGNVG
SVAAKLFQDAGAKVIAVQDHKGIVFNGAGLDVDALIQHVDHNGSVDGFKAETLSADDFWALECEFLIPAALEGQITGKNA
PQIKAKIVVEGANGPTTPEADDILRDRGILVCPDVIANAGGVTVSYFEWVQDFSSFFWTEDEINQRLVRIMQDAFRAIWQ
VAQDNKVTLRTAAFIIACTRILQARQERGLYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=370, Percent_Identity=43.5135135135135, Blast_Score=285, Evalue=6e-77,
Organism=Homo sapiens, GI4885281, Length=369, Percent_Identity=42.2764227642276, Blast_Score=279, Evalue=5e-75,
Organism=Escherichia coli, GI1788059, Length=367, Percent_Identity=33.5149863760218, Blast_Score=182, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17544676, Length=370, Percent_Identity=40, Blast_Score=261, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6319256, Length=383, Percent_Identity=30.0261096605744, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324951, Length=374, Percent_Identity=29.4117647058824, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24649283, Length=463, Percent_Identity=36.5010799136069, Blast_Score=275, Evalue=3e-74,
Organism=Drosophila melanogaster, GI45553475, Length=358, Percent_Identity=42.1787709497207, Blast_Score=274, Evalue=7e-74,
Organism=Drosophila melanogaster, GI45549226, Length=361, Percent_Identity=42.1052631578947, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24649460, Length=311, Percent_Identity=41.4790996784566, Blast_Score=227, Evalue=1e-59,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46823; Mature: 46692

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVASPSTLQHAIPSYLPADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRALIVD
CCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
VPIQMDDGRIAHFEGYRVQHNTSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPY
ECEEECCCEEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCEEECCC
GGAKGGVRVDPRKLSSGELERLTRRYTSEIGIIIGPNKDIPAPDVNTNAQIMAWMMDTYS
CCCCCCCEECCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCEEEEEEEHHHC
MNEGATATGVVTGKPIALGGSLGRREATGRGVFVVGSEAARNLGIDVKGARIVVQGFGNV
CCCCCCEEEEEECCCEEECCCCCCCCCCCCEEEEECCHHHHHCCCCCCCCEEEEEECCCH
GSVAAKLFQDAGAKVIAVQDHKGIVFNGAGLDVDALIQHVDHNGSVDGFKAETLSADDFW
HHHHHHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEE
ALECEFLIPAALEGQITGKNAPQIKAKIVVEGANGPTTPEADDILRDRGILVCPDVIANA
EEEEEEECCEECCCEECCCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCEEECHHHCCC
GGVTVSYFEWVQDFSSFFWTEDEINQRLVRIMQDAFRAIWQVAQDNKVTLRTAAFIIACT
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
RILQARQERGLYP
HHHHHHHHCCCCC
>Mature Secondary Structure 
SNVASPSTLQHAIPSYLPADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRALIVD
CCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
VPIQMDDGRIAHFEGYRVQHNTSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPY
ECEEECCCEEEEEECEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCEEECCC
GGAKGGVRVDPRKLSSGELERLTRRYTSEIGIIIGPNKDIPAPDVNTNAQIMAWMMDTYS
CCCCCCCEECCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCEEEEEEEHHHC
MNEGATATGVVTGKPIALGGSLGRREATGRGVFVVGSEAARNLGIDVKGARIVVQGFGNV
CCCCCCEEEEEECCCEEECCCCCCCCCCCCEEEEECCHHHHHCCCCCCCCEEEEEECCCH
GSVAAKLFQDAGAKVIAVQDHKGIVFNGAGLDVDALIQHVDHNGSVDGFKAETLSADDFW
HHHHHHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEE
ALECEFLIPAALEGQITGKNAPQIKAKIVVEGANGPTTPEADDILRDRGILVCPDVIANA
EEEEEEECCEECCCEECCCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCEEECHHHCCC
GGVTVSYFEWVQDFSSFFWTEDEINQRLVRIMQDAFRAIWQVAQDNKVTLRTAAFIIACT
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
RILQARQERGLYP
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]