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The map label for this gene is aspS

Identifier: 17545185

GI number: 17545185

Start: 497213

End: 499030

Strand: Direct

Name: aspS

Synonym: RSc0466

Alternate gene names: 17545185

Gene position: 497213-499030 (Clockwise)

Preceding gene: 17545184

Following gene: 17545186

Centisome position: 13.38

GC content: 65.13

Gene sequence:

>1818_bases
ATGCGTACTCAATATTGCGGTCAGGTCACCGAACAACTGCTGGGCCAGAGCGTCACCCTGTCGGGCTGGGCGCATCGCCG
GCGTGACCACGGCGGCGTGATCTTCATCGACCTGCGCGATCGCGAGGGCCTGGTGCAGGTGGTGTGCGATCCGGACCGTC
CGGAGATGTTCAAGGTGGCCGAAGGCGTGCGCAACGAGTTCTGCCTGCAGGTCAAGGGCATCGTGCGTGCGCGTCCGGAA
GGCACGACCAACCCGAACCTAGCCAGCGGCAAGATCGAGGTGCTGTGCCATGAGCTGACGGTGCTCAACGCCTCCGTCAC
GCCGCCGTTCCAGCTCGACGACGACAACCTGTCGGAAACCACGCGCCTGACGCACCGCGTGCTGGACCTGCGCCGCCCGC
AGATGCAATACAACCTGCGCCTGCGCTACAAGGTGGCGATGGAAGTGCGCAAGTACCTGGACGACAAGGGCTTCATCGAC
ATCGAAACGCCGATGCTGACCAAGAGCACGCCGGAAGGCGCGCGCGACTACCTGGTGCCGTCGCGCGTGAACGCCGGCCA
GTTCTTCGCGCTGCCGCAGTCGCCGCAGCTGTTCAAGCAGATGCTGATGGTGTCGGGCTTCGACCGCTACTACCAGATCA
CCAAGTGCTTCCGCGACGAAGACCTGCGCGCTGACCGCCAGCCCGAATTCACCCAGATCGACTGCGAAACGTCGTTCCTG
AGCGAGCAGGAAATCCGCGATCTGTTTGAAGAAATGATCCGCACGGTGTTCCAGAACACCATGTCGGTCGCGCTGGATGC
CAAGTTCCCGGTGATGGAGTTCCGCGAGGCGATGGCCCGCTTCGGTTCGGACAAGCCGGACCTGCGCGTGAAGCTGGAGT
TCACCGAGCTGACCGACGCCATGAAAGACGTCGACTTCAAGGTGTTCTCCGGCCCGGCCAACGCCGAAGGCGGCCGCGTG
GTGGCGCTGCGTGTGCCGGGCGGTGCTGCCCTGTCGCGTGGTGACATCGACGCCTACACCAAGTTCGTCGAGATCTACGG
CGCCAAGGGCCTGGCCTGGATCAAGGTCAACGAAGTGGCCAAGGGCCGCGACGGCCTGCAATCGCCGATCGTGAAGAACC
TGCACGACGCGGCCATCGCCGAGATCCTCAAGCGTAGCGGCGCGCAAGACGGCGACATCCTGTTCTTCGGCGCCGACCGC
GCCAAGGTCGTCAACGACGCCATGGGCGCGCTGCGCCTGAAGGTGGGCCATTCGGACTTCGCCAAGAGCACCGGCCTGTT
CGAAGACGCCTGGAAGCCGCTGTGGGTGGTCGACTTCCCGATGTTCGAGTACGACGAGGAAGACGCGCGCTGGGTGGCCA
TGCACCACCCGTTCACGAGTCCGAAGGACGAGCACCTGGAATACCTGGAAACCGATCCGGGCAAGTGCATCGCCAAGGCC
TACGACATGGTGCTCAACGGCTGGGAAATCGGTGGCGGCTCGGTGCGGATCTACCGCGAAGAAGTGCAGAGCAAGGTGTT
CCGCGCCCTGAAGATCGGTGATGAAGAAGCGCGTGCCAAGTTCGGCTTCCTGCTGGACGCCCTGCAGTATGGCGCGCCCC
CGCACGGCGGCATTGCGTTCGGCCTGGACCGCGTTGTCACGATGATGGCCGGCGCCGACTCGATCCGCGACGTGATCGCC
TTCCCGAAGACGCAGCGTGCGCAGGATCTGCTCACGCAGGCGCCGAGCCCGGTCGACGAGAAGCAGCTGCGCGAACTGCA
CATCCGCCTGCGCGCGGCGGAGGCCAAGGTCGCCGCGCCGGCGGCGGCCACGGCCTGA

Upstream 100 bases:

>100_bases
CTGAGCCGGCCGTCCCAACCGATCCACGCCGGCTTCTCGATCAACTTTCACGCGTGTCGCTGTGTTGGCGGCGCGCTTCG
CGGAAAACCACAACATGCAA

Downstream 100 bases:

>100_bases
CCGACGCGCTTCCTGCTGGACGCAAGCCGCGCCGGCCCGCAAGGGCGGCGCGGTTTTTCGTTGGGCCGCGCGGATGCGGC
ATGGGTGGTGCGCGCGTCGC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MRTQYCGQVTEQLLGQSVTLSGWAHRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMFKVAEGVRNEFCLQVKGIVRARPE
GTTNPNLASGKIEVLCHELTVLNASVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDDKGFID
IETPMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQMLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDCETSFL
SEQEIRDLFEEMIRTVFQNTMSVALDAKFPVMEFREAMARFGSDKPDLRVKLEFTELTDAMKDVDFKVFSGPANAEGGRV
VALRVPGGAALSRGDIDAYTKFVEIYGAKGLAWIKVNEVAKGRDGLQSPIVKNLHDAAIAEILKRSGAQDGDILFFGADR
AKVVNDAMGALRLKVGHSDFAKSTGLFEDAWKPLWVVDFPMFEYDEEDARWVAMHHPFTSPKDEHLEYLETDPGKCIAKA
YDMVLNGWEIGGGSVRIYREEVQSKVFRALKIGDEEARAKFGFLLDALQYGAPPHGGIAFGLDRVVTMMAGADSIRDVIA
FPKTQRAQDLLTQAPSPVDEKQLRELHIRLRAAEAKVAAPAAATA

Sequences:

>Translated_605_residues
MRTQYCGQVTEQLLGQSVTLSGWAHRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMFKVAEGVRNEFCLQVKGIVRARPE
GTTNPNLASGKIEVLCHELTVLNASVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDDKGFID
IETPMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQMLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDCETSFL
SEQEIRDLFEEMIRTVFQNTMSVALDAKFPVMEFREAMARFGSDKPDLRVKLEFTELTDAMKDVDFKVFSGPANAEGGRV
VALRVPGGAALSRGDIDAYTKFVEIYGAKGLAWIKVNEVAKGRDGLQSPIVKNLHDAAIAEILKRSGAQDGDILFFGADR
AKVVNDAMGALRLKVGHSDFAKSTGLFEDAWKPLWVVDFPMFEYDEEDARWVAMHHPFTSPKDEHLEYLETDPGKCIAKA
YDMVLNGWEIGGGSVRIYREEVQSKVFRALKIGDEEARAKFGFLLDALQYGAPPHGGIAFGLDRVVTMMAGADSIRDVIA
FPKTQRAQDLLTQAPSPVDEKQLRELHIRLRAAEAKVAAPAAATA
>Mature_605_residues
MRTQYCGQVTEQLLGQSVTLSGWAHRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMFKVAEGVRNEFCLQVKGIVRARPE
GTTNPNLASGKIEVLCHELTVLNASVTPPFQLDDDNLSETTRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDDKGFID
IETPMLTKSTPEGARDYLVPSRVNAGQFFALPQSPQLFKQMLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDCETSFL
SEQEIRDLFEEMIRTVFQNTMSVALDAKFPVMEFREAMARFGSDKPDLRVKLEFTELTDAMKDVDFKVFSGPANAEGGRV
VALRVPGGAALSRGDIDAYTKFVEIYGAKGLAWIKVNEVAKGRDGLQSPIVKNLHDAAIAEILKRSGAQDGDILFFGADR
AKVVNDAMGALRLKVGHSDFAKSTGLFEDAWKPLWVVDFPMFEYDEEDARWVAMHHPFTSPKDEHLEYLETDPGKCIAKA
YDMVLNGWEIGGGSVRIYREEVQSKVFRALKIGDEEARAKFGFLLDALQYGAPPHGGIAFGLDRVVTMMAGADSIRDVIA
FPKTQRAQDLLTQAPSPVDEKQLRELHIRLRAAEAKVAAPAAATA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=604, Percent_Identity=39.4039735099338, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI45439306, Length=271, Percent_Identity=28.7822878228782, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI194272210, Length=253, Percent_Identity=24.5059288537549, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI5031815, Length=253, Percent_Identity=24.5059288537549, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=59.0524534686971, Blast_Score=706, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=297, Percent_Identity=25.2525252525253, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1790571, Length=255, Percent_Identity=26.2745098039216, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=607, Percent_Identity=33.2784184514003, Blast_Score=317, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17551876, Length=270, Percent_Identity=26.2962962962963, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71994340, Length=262, Percent_Identity=24.0458015267176, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=262, Percent_Identity=23.6641221374046, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=262, Percent_Identity=23.6641221374046, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=635, Percent_Identity=34.488188976378, Blast_Score=291, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6323011, Length=290, Percent_Identity=28.2758620689655, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324256, Length=183, Percent_Identity=28.4153005464481, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321807, Length=254, Percent_Identity=26.3779527559055, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=265, Percent_Identity=24.9056603773585, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24584738, Length=599, Percent_Identity=37.228714524207, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17136276, Length=283, Percent_Identity=27.5618374558304, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24640849, Length=249, Percent_Identity=26.1044176706827, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24640851, Length=249, Percent_Identity=26.1044176706827, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_RALSO (Q8Y270)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518587.1
- ProteinModelPortal:   Q8Y270
- SMR:   Q8Y270
- GeneID:   1219270
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0466
- NMPDR:   fig|267608.1.peg.466
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   RSOL267608:RSC0466-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67957; Mature: 67957

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTQYCGQVTEQLLGQSVTLSGWAHRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMFKVA
CCCCHHHHHHHHHCCCCEEECCHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHH
EGVRNEFCLQVKGIVRARPEGTTNPNLASGKIEVLCHELTVLNASVTPPFQLDDDNLSET
HHHHHHHHHEEHHHHEECCCCCCCCCCCCCHHEEEEEEHEEEECCCCCCCCCCCCCCHHH
TRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDDKGFIDIETPMLTKSTPEGARDYLVP
HHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHCCCC
SRVNAGQFFALPQSPQLFKQMLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDCETSFL
CCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHC
SEQEIRDLFEEMIRTVFQNTMSVALDAKFPVMEFREAMARFGSDKPDLRVKLEFTELTDA
CHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHH
MKDVDFKVFSGPANAEGGRVVALRVPGGAALSRGDIDAYTKFVEIYGAKGLAWIKVNEVA
HHHCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCHH
KGRDGLQSPIVKNLHDAAIAEILKRSGAQDGDILFFGADRAKVVNDAMGALRLKVGHSDF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHEEEEECCCHH
AKSTGLFEDAWKPLWVVDFPMFEYDEEDARWVAMHHPFTSPKDEHLEYLETDPGKCIAKA
HHHCCCHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCHHHHHH
YDMVLNGWEIGGGSVRIYREEVQSKVFRALKIGDEEARAKFGFLLDALQYGAPPHGGIAF
HHHHHCCEEECCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
GLDRVVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSPVDEKQLRELHIRLRAAEAKVAAP
HHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
AAATA
CCCCC
>Mature Secondary Structure
MRTQYCGQVTEQLLGQSVTLSGWAHRRRDHGGVIFIDLRDREGLVQVVCDPDRPEMFKVA
CCCCHHHHHHHHHCCCCEEECCHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCHHHHHH
EGVRNEFCLQVKGIVRARPEGTTNPNLASGKIEVLCHELTVLNASVTPPFQLDDDNLSET
HHHHHHHHHEEHHHHEECCCCCCCCCCCCCHHEEEEEEHEEEECCCCCCCCCCCCCCHHH
TRLTHRVLDLRRPQMQYNLRLRYKVAMEVRKYLDDKGFIDIETPMLTKSTPEGARDYLVP
HHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHCCCC
SRVNAGQFFALPQSPQLFKQMLMVSGFDRYYQITKCFRDEDLRADRQPEFTQIDCETSFL
CCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHC
SEQEIRDLFEEMIRTVFQNTMSVALDAKFPVMEFREAMARFGSDKPDLRVKLEFTELTDA
CHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHH
MKDVDFKVFSGPANAEGGRVVALRVPGGAALSRGDIDAYTKFVEIYGAKGLAWIKVNEVA
HHHCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCHH
KGRDGLQSPIVKNLHDAAIAEILKRSGAQDGDILFFGADRAKVVNDAMGALRLKVGHSDF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHEEEEECCCHH
AKSTGLFEDAWKPLWVVDFPMFEYDEEDARWVAMHHPFTSPKDEHLEYLETDPGKCIAKA
HHHCCCHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCHHHHHH
YDMVLNGWEIGGGSVRIYREEVQSKVFRALKIGDEEARAKFGFLLDALQYGAPPHGGIAF
HHHHHCCEEECCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
GLDRVVTMMAGADSIRDVIAFPKTQRAQDLLTQAPSPVDEKQLRELHIRLRAAEAKVAAP
HHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
AAATA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852