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The map label for this gene is ytcB [H]

Identifier: 17231001

GI number: 17231001

Start: 4228667

End: 4229617

Strand: Reverse

Name: ytcB [H]

Synonym: all3509

Alternate gene names: 17231001

Gene position: 4229617-4228667 (Counterclockwise)

Preceding gene: 17231007

Following gene: 17230995

Centisome position: 65.95

GC content: 44.27

Gene sequence:

>951_bases
ATGGCGAAAATTATAGTTACTGGAGCAGCAGGATTTATTGCTTCTCACCTGGTAGAAACCCTACTCAAACAAGGTGAAGA
GGTAATCGGGATTGATGAAGTGAATGATTACTACGATCCCTTATTAAAACGTAAAAATATTGCCCATTTACAATCGTTTC
CTAACTTCACCTTCATTGAGGGAGATATTCAGTTTCTAGATTGGCCGTCCCTACTCCAAGATGTGACTGTGGTCTACCAT
CAGGCGGCGCAGGCGGGAGTTAGGGCAAGTTGGGGTAATGGTTTCCGTGCTTATACTGAGCGCAATATTAATGCTACACA
AGTTTTGTTGGAAGCAGCTAAGGATGCTCAACAATTAACTAGGTTAGTCTTTGCATCTACTTCTAGTGTGTATGGGGATG
CGGAAACCTTACCTACTCACGAAGGGATTCCGCCTCAACCGGTTTCTCCTTATGGCATTACTAAGTTAGCTGCCGAGCGT
TTGTGTGGGCTATATCACAAAAATTTCGGTGTACCGTTTGTGGCTCTACGTTACTTTACTGTATATGGCCCTAGACAGCG
CCCTGATATGGCTTTCCATAAGTTTTTTAAATCTGTTTTACAGGATGAGGCGATTCCTGTTTACGGAGATGGACAGCAAA
CTCGTGACTTTACTTTTGTGAGTGATGCTGTAGCGGCAAATTTAGCGGCTGCTAGTGTACCGGCTGCTGTAGGAGAAATT
TTTAATATTGGTGGTGGTAGTAGGGTGGTTTTGGCTGAGGTCTTAGACACGATGGAACAAATCGTTGGTCAGCCAATTAA
GAGAAATCACATAGAAAAGGCAATGGGTGATGCACGTCACACGGCTGCTGATGTGTCGAAGGCACGGAAAATTTTAGGAT
ATGAGCCAAAGGTTTCTTTGAGGGAGGGTTTGAGCTTAGAATGGCAGTGGGTGAAGGCTTTGTACACCTAG

Upstream 100 bases:

>100_bases
TGATACGGTGGGCATTGCCCACCCTACAGATACTTAGATTTGTTCAAGAATCAAATCGGATTCCTATAGAAACTAAGAAA
ATAAGAATAAAGTAGGTAAA

Downstream 100 bases:

>100_bases
AGCCAGTGAAAGCGATCGCCTGGATTAAACTAAGAGGGTTTAGATAAAGCGATCGCACTAATTCCACTCAACAGCAAACC
TATGCCTATCCATTGGGAAA

Product: nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MAKIIVTGAAGFIASHLVETLLKQGEEVIGIDEVNDYYDPLLKRKNIAHLQSFPNFTFIEGDIQFLDWPSLLQDVTVVYH
QAAQAGVRASWGNGFRAYTERNINATQVLLEAAKDAQQLTRLVFASTSSVYGDAETLPTHEGIPPQPVSPYGITKLAAER
LCGLYHKNFGVPFVALRYFTVYGPRQRPDMAFHKFFKSVLQDEAIPVYGDGQQTRDFTFVSDAVAANLAAASVPAAVGEI
FNIGGGSRVVLAEVLDTMEQIVGQPIKRNHIEKAMGDARHTAADVSKARKILGYEPKVSLREGLSLEWQWVKALYT

Sequences:

>Translated_316_residues
MAKIIVTGAAGFIASHLVETLLKQGEEVIGIDEVNDYYDPLLKRKNIAHLQSFPNFTFIEGDIQFLDWPSLLQDVTVVYH
QAAQAGVRASWGNGFRAYTERNINATQVLLEAAKDAQQLTRLVFASTSSVYGDAETLPTHEGIPPQPVSPYGITKLAAER
LCGLYHKNFGVPFVALRYFTVYGPRQRPDMAFHKFFKSVLQDEAIPVYGDGQQTRDFTFVSDAVAANLAAASVPAAVGEI
FNIGGGSRVVLAEVLDTMEQIVGQPIKRNHIEKAMGDARHTAADVSKARKILGYEPKVSLREGLSLEWQWVKALYT
>Mature_315_residues
AKIIVTGAAGFIASHLVETLLKQGEEVIGIDEVNDYYDPLLKRKNIAHLQSFPNFTFIEGDIQFLDWPSLLQDVTVVYHQ
AAQAGVRASWGNGFRAYTERNINATQVLLEAAKDAQQLTRLVFASTSSVYGDAETLPTHEGIPPQPVSPYGITKLAAERL
CGLYHKNFGVPFVALRYFTVYGPRQRPDMAFHKFFKSVLQDEAIPVYGDGQQTRDFTFVSDAVAANLAAASVPAAVGEIF
NIGGGSRVVLAEVLDTMEQIVGQPIKRNHIEKAMGDARHTAADVSKARKILGYEPKVSLREGLSLEWQWVKALYT

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=323, Percent_Identity=31.2693498452012, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI42516563, Length=310, Percent_Identity=31.2903225806452, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI56237023, Length=337, Percent_Identity=26.7062314540059, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI56118217, Length=337, Percent_Identity=26.7062314540059, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI189083684, Length=337, Percent_Identity=26.7062314540059, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI48994969, Length=341, Percent_Identity=29.0322580645161, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788353, Length=350, Percent_Identity=28.5714285714286, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1786974, Length=337, Percent_Identity=27.299703264095, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788589, Length=341, Percent_Identity=24.3401759530792, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=321, Percent_Identity=28.3489096573209, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17539532, Length=324, Percent_Identity=28.3950617283951, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI115532424, Length=322, Percent_Identity=24.5341614906832, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71982035, Length=344, Percent_Identity=26.453488372093, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=346, Percent_Identity=26.5895953757225, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=338, Percent_Identity=26.3313609467456, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21356223, Length=312, Percent_Identity=31.4102564102564, Blast_Score=127, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 34829; Mature: 34698

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIIVTGAAGFIASHLVETLLKQGEEVIGIDEVNDYYDPLLKRKNIAHLQSFPNFTFIE
CCEEEEECHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCHHHHHHCCCCEEEE
GDIQFLDWPSLLQDVTVVYHQAAQAGVRASWGNGFRAYTERNINATQVLLEAAKDAQQLT
CCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHH
RLVFASTSSVYGDAETLPTHEGIPPQPVSPYGITKLAAERLCGLYHKNFGVPFVALRYFT
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VYGPRQRPDMAFHKFFKSVLQDEAIPVYGDGQQTRDFTFVSDAVAANLAAASVPAAVGEI
HCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FNIGGGSRVVLAEVLDTMEQIVGQPIKRNHIEKAMGDARHTAADVSKARKILGYEPKVSL
HCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
REGLSLEWQWVKALYT
HCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AKIIVTGAAGFIASHLVETLLKQGEEVIGIDEVNDYYDPLLKRKNIAHLQSFPNFTFIE
CEEEEECHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCHHHHHHCCCCEEEE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HCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]