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The map label for this gene is braE [H]

Identifier: 17230030

GI number: 17230030

Start: 3043342

End: 3044268

Strand: Direct

Name: braE [H]

Synonym: alr2538

Alternate gene names: 17230030

Gene position: 3043342-3044268 (Clockwise)

Preceding gene: 17230029

Following gene: 17230031

Centisome position: 47.45

GC content: 40.13

Gene sequence:

>927_bases
ATGGCTGAATTTTTCTCCACCTATGGTTCTCTCATCGTCTCTATGGTGTTGGGGGCGTTACTGGGACTATCACTTTACTT
ACCTCTTATGGCGGGGCAATTATCTTTAGCAAGCCCTGGATTTTATGCTTTAGGTGGTTATATTGCCGCGATTTTATCAA
CCAAAGTTTTTCAAACTAACAGTAATTCATTTCCTATACCGCTACTTTTATTGGAAATGTTAATTGCTGCTGTAGTCTCT
GGTTTATTAGGGATAGCTGTAGGTATTCCAGCTTTACGTTTGCGGGGAATTTATTTGGCGATCGCTACTATCGCTTTTGT
GGAAGTTTTACGCGTTCTCTCCCTAAATTTAGATATTACAGGCGGTGCTGTCGGTATTTTTGGTATCCCTCAACCCTTCC
AAACACCAATCGAATATTTATGGATTACTTTGCCACTACTATTAATTAGTATGGTGCTATTTTACCGTTTAGAACGGGTG
CGAGTAGGTAGAGCTTTTACTGCCATCCGTGAAGATGAATTAGCAGCCGGGGCGATGGGGATTAACCCAACTTACTACAA
AGTTTTAGCATTTACTTTAGGGGCGATGCTTGCTGGTATTGTGGGTGTGATTAGCGCCCACTTTCTTAATACTTGGAATG
CCAGACAAGGAACATTTGATGCTAGTATTATCTACCTCACTTTTGTTTTAATTGGTGGTTCGAGAACTTTTTTAGGTTCA
GTTGTTGGTGGTATGGTATTTACAGCTTTACCAGAAGTTTTACGCGGACTAGCTGATACAGGTGGTTTTCCTACTTGGTT
AGCCCAGTTTCTCCGGGATGGTAGGTTAATTATTTTTGGGTTGTTAATAGTTACAGGTACTATTTTCTTTCCTCAGGGTT
TGGTAACTCCAGATATTTTTAAATTCGGTAAGCCTAAAAATAGATGA

Upstream 100 bases:

>100_bases
TTTAAAGGTTCTGAGCGTATTATATCAGTCACCTTTCCTGAACTAACATTAACCGCAGAGCAAATATTAACAGCTTAAAG
TTTCATAAAAGCAATTTAAA

Downstream 100 bases:

>100_bases
GCCTAGATAAAAGTAGAGTTATATTAGAAGCAAAGTCACTCACACGCCGCTTTGGTGGTTTAGTAGCGGTAAATAATGTA
TCTTTTACAGTTAATCACTA

Product: permease protein of branched-chain amino acid ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MAEFFSTYGSLIVSMVLGALLGLSLYLPLMAGQLSLASPGFYALGGYIAAILSTKVFQTNSNSFPIPLLLLEMLIAAVVS
GLLGIAVGIPALRLRGIYLAIATIAFVEVLRVLSLNLDITGGAVGIFGIPQPFQTPIEYLWITLPLLLISMVLFYRLERV
RVGRAFTAIREDELAAGAMGINPTYYKVLAFTLGAMLAGIVGVISAHFLNTWNARQGTFDASIIYLTFVLIGGSRTFLGS
VVGGMVFTALPEVLRGLADTGGFPTWLAQFLRDGRLIIFGLLIVTGTIFFPQGLVTPDIFKFGKPKNR

Sequences:

>Translated_308_residues
MAEFFSTYGSLIVSMVLGALLGLSLYLPLMAGQLSLASPGFYALGGYIAAILSTKVFQTNSNSFPIPLLLLEMLIAAVVS
GLLGIAVGIPALRLRGIYLAIATIAFVEVLRVLSLNLDITGGAVGIFGIPQPFQTPIEYLWITLPLLLISMVLFYRLERV
RVGRAFTAIREDELAAGAMGINPTYYKVLAFTLGAMLAGIVGVISAHFLNTWNARQGTFDASIIYLTFVLIGGSRTFLGS
VVGGMVFTALPEVLRGLADTGGFPTWLAQFLRDGRLIIFGLLIVTGTIFFPQGLVTPDIFKFGKPKNR
>Mature_307_residues
AEFFSTYGSLIVSMVLGALLGLSLYLPLMAGQLSLASPGFYALGGYIAAILSTKVFQTNSNSFPIPLLLLEMLIAAVVSG
LLGIAVGIPALRLRGIYLAIATIAFVEVLRVLSLNLDITGGAVGIFGIPQPFQTPIEYLWITLPLLLISMVLFYRLERVR
VGRAFTAIREDELAAGAMGINPTYYKVLAFTLGAMLAGIVGVISAHFLNTWNARQGTFDASIIYLTFVLIGGSRTFLGSV
VGGMVFTALPEVLRGLADTGGFPTWLAQFLRDGRLIIFGLLIVTGTIFFPQGLVTPDIFKFGKPKNR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=314, Percent_Identity=30.5732484076433, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 33064; Mature: 32933

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEFFSTYGSLIVSMVLGALLGLSLYLPLMAGQLSLASPGFYALGGYIAAILSTKVFQTN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
SNSFPIPLLLLEMLIAAVVSGLLGIAVGIPALRLRGIYLAIATIAFVEVLRVLSLNLDIT
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEC
GGAVGIFGIPQPFQTPIEYLWITLPLLLISMVLFYRLERVRVGRAFTAIREDELAAGAMG
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
INPTYYKVLAFTLGAMLAGIVGVISAHFLNTWNARQGTFDASIIYLTFVLIGGSRTFLGS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
VVGGMVFTALPEVLRGLADTGGFPTWLAQFLRDGRLIIFGLLIVTGTIFFPQGLVTPDIF
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHH
KFGKPKNR
HCCCCCCC
>Mature Secondary Structure 
AEFFSTYGSLIVSMVLGALLGLSLYLPLMAGQLSLASPGFYALGGYIAAILSTKVFQTN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
SNSFPIPLLLLEMLIAAVVSGLLGIAVGIPALRLRGIYLAIATIAFVEVLRVLSLNLDIT
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEC
GGAVGIFGIPQPFQTPIEYLWITLPLLLISMVLFYRLERVRVGRAFTAIREDELAAGAMG
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
INPTYYKVLAFTLGAMLAGIVGVISAHFLNTWNARQGTFDASIIYLTFVLIGGSRTFLGS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
VVGGMVFTALPEVLRGLADTGGFPTWLAQFLRDGRLIIFGLLIVTGTIFFPQGLVTPDIF
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHH
KFGKPKNR
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]