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The map label for this gene is lacF [H]

Identifier: 17230024

GI number: 17230024

Start: 3036844

End: 3037749

Strand: Direct

Name: lacF [H]

Synonym: alr2532

Alternate gene names: 17230024

Gene position: 3036844-3037749 (Clockwise)

Preceding gene: 17230019

Following gene: 17230027

Centisome position: 47.35

GC content: 40.4

Gene sequence:

>906_bases
ATGGTGTTGCGAATCAGAAGGTGGAGAAATAACCATAGATGGAACATCACAGACAGCTTGTTCGGATATGTATTCATGAT
GCCGACTCTGCTGGTTTTGGGAACTTTCGTAGTTCTACCTATTCTCTATTCTGTTTTCCTGTCGCTCAACAAAGTTCAAC
TGTTAGGTGGTGTTGCCTATCAATTCATTGGGTTGCGAAATTTTCAGCGATTAGTTGACGATGAATTAGTTTGGATTGCC
TTAAGAAATACAGCAGAATATGTGGTCATTGTTGTACCAAGCCAAACTGTTTTGGCATTGATTTTAGCAGTAACTTTAAA
TGCTGGAATCCGGGGTAAGAACTGGTGGCGCATCCTTTACTTTTTGCCAACAGTCACTTCTTCAGCAGTGCTGACGCTAA
TTTTTATGTGGATTTATAATACAGATGGACTATTAAATGATTTTCTAGCTTTTTTGGGACTGCCTACTTATAATTGGTTG
GGTGATCCGGCTGTTGCCCTCAAAGGCATCATGCTCATGAATATTTGGTCAACTGCACCATTTTATATGGTGATATATCT
AGCAGCCTTACAAGATATACCTGCGAAACTTTACGAGGCGGCAGAATTAGATGGTGCAAATTGGTGGCAGCAATTTATTT
ACATTACAATTCCTCTGCTGAAACCTGTGACTTTTTTTGTGATCGCGGTGGGGGTAATTGGCACTTTTCAATTATTTGAT
CAATCTTATATTTTCTCTGGTGGTACTGGCGGCCCTAATAATGCCACCTTGACTGTAGTGCTGCTGATTTATCAAATGGT
ATTTCGTTATTTACAGATGGGATATGCTGCGGCGATCGCCTTTCTCTTGGCAGTGGTGATTATTGGTATTACACTGATTC
AACGGCGAGTTTTTGCTGGTGAATAA

Upstream 100 bases:

>100_bases
AAATATGGAAAAATCATCCATAGGGTATCTATGAGTCAACTCATCCCTTCCACTACGATAGCGATCGCACAGTACCCACA
GCTAGTTATAGGCAGATTAG

Downstream 100 bases:

>100_bases
TTCCCAGTTTATGATCGGGAATAACAATATTTAGGACTAACGCACCCAGGCTGTTTGTTGAGACTAGATATAAGGGGTTA
AGGACACAAAGGTGTAAGGA

Product: sugar ABC transport system, permease protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MVLRIRRWRNNHRWNITDSLFGYVFMMPTLLVLGTFVVLPILYSVFLSLNKVQLLGGVAYQFIGLRNFQRLVDDELVWIA
LRNTAEYVVIVVPSQTVLALILAVTLNAGIRGKNWWRILYFLPTVTSSAVLTLIFMWIYNTDGLLNDFLAFLGLPTYNWL
GDPAVALKGIMLMNIWSTAPFYMVIYLAALQDIPAKLYEAAELDGANWWQQFIYITIPLLKPVTFFVIAVGVIGTFQLFD
QSYIFSGGTGGPNNATLTVVLLIYQMVFRYLQMGYAAAIAFLLAVVIIGITLIQRRVFAGE

Sequences:

>Translated_301_residues
MVLRIRRWRNNHRWNITDSLFGYVFMMPTLLVLGTFVVLPILYSVFLSLNKVQLLGGVAYQFIGLRNFQRLVDDELVWIA
LRNTAEYVVIVVPSQTVLALILAVTLNAGIRGKNWWRILYFLPTVTSSAVLTLIFMWIYNTDGLLNDFLAFLGLPTYNWL
GDPAVALKGIMLMNIWSTAPFYMVIYLAALQDIPAKLYEAAELDGANWWQQFIYITIPLLKPVTFFVIAVGVIGTFQLFD
QSYIFSGGTGGPNNATLTVVLLIYQMVFRYLQMGYAAAIAFLLAVVIIGITLIQRRVFAGE
>Mature_301_residues
MVLRIRRWRNNHRWNITDSLFGYVFMMPTLLVLGTFVVLPILYSVFLSLNKVQLLGGVAYQFIGLRNFQRLVDDELVWIA
LRNTAEYVVIVVPSQTVLALILAVTLNAGIRGKNWWRILYFLPTVTSSAVLTLIFMWIYNTDGLLNDFLAFLGLPTYNWL
GDPAVALKGIMLMNIWSTAPFYMVIYLAALQDIPAKLYEAAELDGANWWQQFIYITIPLLKPVTFFVIAVGVIGTFQLFD
QSYIFSGGTGGPNNATLTVVLLIYQMVFRYLQMGYAAAIAFLLAVVIIGITLIQRRVFAGE

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787570, Length=267, Percent_Identity=30.7116104868914, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789861, Length=278, Percent_Identity=29.8561151079137, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790465, Length=254, Percent_Identity=29.5275590551181, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34061; Mature: 34061

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLRIRRWRNNHRWNITDSLFGYVFMMPTLLVLGTFVVLPILYSVFLSLNKVQLLGGVAY
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFIGLRNFQRLVDDELVWIALRNTAEYVVIVVPSQTVLALILAVTLNAGIRGKNWWRILY
HHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCEEEEE
FLPTVTSSAVLTLIFMWIYNTDGLLNDFLAFLGLPTYNWLGDPAVALKGIMLMNIWSTAP
EHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCH
FYMVIYLAALQDIPAKLYEAAELDGANWWQQFIYITIPLLKPVTFFVIAVGVIGTFQLFD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QSYIFSGGTGGPNNATLTVVLLIYQMVFRYLQMGYAAAIAFLLAVVIIGITLIQRRVFAG
CHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
E
C
>Mature Secondary Structure
MVLRIRRWRNNHRWNITDSLFGYVFMMPTLLVLGTFVVLPILYSVFLSLNKVQLLGGVAY
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QFIGLRNFQRLVDDELVWIALRNTAEYVVIVVPSQTVLALILAVTLNAGIRGKNWWRILY
HHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCEEEEE
FLPTVTSSAVLTLIFMWIYNTDGLLNDFLAFLGLPTYNWLGDPAVALKGIMLMNIWSTAP
EHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCH
FYMVIYLAALQDIPAKLYEAAELDGANWWQQFIYITIPLLKPVTFFVIAVGVIGTFQLFD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QSYIFSGGTGGPNNATLTVVLLIYQMVFRYLQMGYAAAIAFLLAVVIIGITLIQRRVFAG
CHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]