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The map label for this gene is gatB [H]

Identifier: 17228891

GI number: 17228891

Start: 1657681

End: 1659156

Strand: Direct

Name: gatB [H]

Synonym: alr1396

Alternate gene names: 17228891

Gene position: 1657681-1659156 (Clockwise)

Preceding gene: 17228888

Following gene: 17228892

Centisome position: 25.85

GC content: 44.38

Gene sequence:

>1476_bases
ATGACTTCTGCTACGACCGTAAAAACTGAGTACGAAGCGATTATCGGTCTAGAAACCCATTGTCAGCTGAGTACGAATAC
CAAAATTTTCTCTAGTAGCTCAACAGCGTTCGGGGCTGACCCTAATACGAACATTGACCCGGTGTGTATGGGCTTACCTG
GTGTTTTGCCTGTACTTAACGAAAAAGTCTTAGAATACGCTGTTAAAGCAGGTTTGGCGTTGAATTGCCAAATTGCTAAA
TATAGTAAATTTGACCGTAAACAGTATTTTTATCCTGATTTACCGAAAAATTATCAAATTTCTCAATATGACTTACCCAT
AGCTGAACATGGCTGGTTAGAAATTGAGTTATTAGATGCCGATGGTAACCCAAAACGTAAGCGGATAGGGATTACCCGTC
TGCACATGGAAGAAGACGCAGGGAAACTGGTACACGCAGGGAGCGATCGCATCTCTGGTTCTACCTATTCTCTGGTAGAC
TACAACCGTGCGGGTGTGCCATTGGTAGAAATTGTCTCGGAACCAGACATCCGCACTGGGCAAGAAGCTGCTGAATATGC
CCAAGAATTACGCCGGGTGATGCGTTATCTCGGTGTCAGTGATGGCAATATGCAGGAAGGTTCTCTGCGCTGTGATGTGA
ATATATCTGTGCGTCCTGTAGGACAGGAAAAGTTCGGCACGAAGGTAGAAATTAAAAACATGAACTCCTTCAGCGCCATT
CAAAAAGCCATTGAACACGAAATTGAACGGCAAATAGAAGCGATAGAGTCAGGGGAAAAGATTATTCAAGAAACGCGACT
GTGGGAAGAAGGTTCACAACGCACAATTAGTATGCGAATTAAAGAAGGTTCTAGCGATTATCGCTACTTCCCCGAACCGG
ATTTAGCACCCATTGAGGTAACAGAAGCACAACTGAGTCAATGGCGTAGTGAATTACCAGAACTACCAGCCCAAAAACGC
CATCGTTACGAAAACGAATTGGGTTTGTCGGCTTACGATACCCGCGTGCTGACAGAAGATGTCATTGTATCTCAATATTT
TGAAGTAGCGATCGCATCTGGGGCAAATCCCAAAGCAGCCGCTAACTGGATTACCCAAGATATCGCCGCCTATCTCAACA
AACAAAAACTCAGCATTACAGAAATCGGCTTAACTCCCGCTAACTTAGCCGATGTGATTACCCGGATTGAGACTGGCAAA
ATTAGCAACGCTCAAGCTAAACAAAAGTTACCAGAGTTACTCACGGGATTATCCCCGGAAAAAGCCTTTGCAGGTCAAGA
ACTCATCAGCGACCCCAGTGTTTTGGAACCCATCGTTGATGAAGTGATAGCAGCTAACCCCAAAGAACTGGAAAAGTACC
GCAACGGTAACATCAATCTTAAGGGTTTCTTTGTCGGGCAAGTGCTGAAAAAGACAAATAAACGCGCTGACCCCAAACTC
ACCAACGAATTGGTAGAAAATAAACTAAATGGCTAA

Upstream 100 bases:

>100_bases
AGATTTAAGCCCAGGTTCTGGCTGACACAGGAAGCAAAGAAATTGTCGATGCGAGGCTAAAATAGTAATCTACGTGTAAA
TTGCTTGAACTATTGATTTT

Downstream 100 bases:

>100_bases
CACAAGTATTTTGTCAGTCATTTTTTCACAATAGTTTTACATAAAGTACAAAAAAGTGGGTGTCGATAATTACAACTAAG
CGGTTATTATGAATGTATTA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MTSATTVKTEYEAIIGLETHCQLSTNTKIFSSSSTAFGADPNTNIDPVCMGLPGVLPVLNEKVLEYAVKAGLALNCQIAK
YSKFDRKQYFYPDLPKNYQISQYDLPIAEHGWLEIELLDADGNPKRKRIGITRLHMEEDAGKLVHAGSDRISGSTYSLVD
YNRAGVPLVEIVSEPDIRTGQEAAEYAQELRRVMRYLGVSDGNMQEGSLRCDVNISVRPVGQEKFGTKVEIKNMNSFSAI
QKAIEHEIERQIEAIESGEKIIQETRLWEEGSQRTISMRIKEGSSDYRYFPEPDLAPIEVTEAQLSQWRSELPELPAQKR
HRYENELGLSAYDTRVLTEDVIVSQYFEVAIASGANPKAAANWITQDIAAYLNKQKLSITEIGLTPANLADVITRIETGK
ISNAQAKQKLPELLTGLSPEKAFAGQELISDPSVLEPIVDEVIAANPKELEKYRNGNINLKGFFVGQVLKKTNKRADPKL
TNELVENKLNG

Sequences:

>Translated_491_residues
MTSATTVKTEYEAIIGLETHCQLSTNTKIFSSSSTAFGADPNTNIDPVCMGLPGVLPVLNEKVLEYAVKAGLALNCQIAK
YSKFDRKQYFYPDLPKNYQISQYDLPIAEHGWLEIELLDADGNPKRKRIGITRLHMEEDAGKLVHAGSDRISGSTYSLVD
YNRAGVPLVEIVSEPDIRTGQEAAEYAQELRRVMRYLGVSDGNMQEGSLRCDVNISVRPVGQEKFGTKVEIKNMNSFSAI
QKAIEHEIERQIEAIESGEKIIQETRLWEEGSQRTISMRIKEGSSDYRYFPEPDLAPIEVTEAQLSQWRSELPELPAQKR
HRYENELGLSAYDTRVLTEDVIVSQYFEVAIASGANPKAAANWITQDIAAYLNKQKLSITEIGLTPANLADVITRIETGK
ISNAQAKQKLPELLTGLSPEKAFAGQELISDPSVLEPIVDEVIAANPKELEKYRNGNINLKGFFVGQVLKKTNKRADPKL
TNELVENKLNG
>Mature_490_residues
TSATTVKTEYEAIIGLETHCQLSTNTKIFSSSSTAFGADPNTNIDPVCMGLPGVLPVLNEKVLEYAVKAGLALNCQIAKY
SKFDRKQYFYPDLPKNYQISQYDLPIAEHGWLEIELLDADGNPKRKRIGITRLHMEEDAGKLVHAGSDRISGSTYSLVDY
NRAGVPLVEIVSEPDIRTGQEAAEYAQELRRVMRYLGVSDGNMQEGSLRCDVNISVRPVGQEKFGTKVEIKNMNSFSAIQ
KAIEHEIERQIEAIESGEKIIQETRLWEEGSQRTISMRIKEGSSDYRYFPEPDLAPIEVTEAQLSQWRSELPELPAQKRH
RYENELGLSAYDTRVLTEDVIVSQYFEVAIASGANPKAAANWITQDIAAYLNKQKLSITEIGLTPANLADVITRIETGKI
SNAQAKQKLPELLTGLSPEKAFAGQELISDPSVLEPIVDEVIAANPKELEKYRNGNINLKGFFVGQVLKKTNKRADPKLT
NELVENKLNG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=511, Percent_Identity=38.5518590998043, Blast_Score=326, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI115534094, Length=315, Percent_Identity=39.6825396825397, Blast_Score=224, Evalue=7e-59,
Organism=Saccharomyces cerevisiae, GI6319391, Length=435, Percent_Identity=32.6436781609195, Blast_Score=211, Evalue=1e-55,
Organism=Drosophila melanogaster, GI45550808, Length=503, Percent_Identity=34.3936381709742, Blast_Score=288, Evalue=5e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54694; Mature: 54562

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSATTVKTEYEAIIGLETHCQLSTNTKIFSSSSTAFGADPNTNIDPVCMGLPGVLPVLN
CCCCCEEEEHHHHEEEECCEEEECCCCEEEECCCCCCCCCCCCCCCCEEECCCCHHHHHH
EKVLEYAVKAGLALNCQIAKYSKFDRKQYFYPDLPKNYQISQYDLPIAEHGWLEIELLDA
HHHHHHHHHCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEEECC
DGNPKRKRIGITRLHMEEDAGKLVHAGSDRISGSTYSLVDYNRAGVPLVEIVSEPDIRTG
CCCCCHHHCCEEEEEECCCCCCEEECCCCCCCCCEEEEEECCCCCCCHHEECCCCCCCCC
QEAAEYAQELRRVMRYLGVSDGNMQEGSLRCDVNISVRPVGQEKFGTKVEIKNMNSFSAI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCHHHCCCEEEEECCCHHHHH
QKAIEHEIERQIEAIESGEKIIQETRLWEEGSQRTISMRIKEGSSDYRYFPEPDLAPIEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCEE
TEAQLSQWRSELPELPAQKRHRYENELGLSAYDTRVLTEDVIVSQYFEVAIASGANPKAA
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
ANWITQDIAAYLNKQKLSITEIGLTPANLADVITRIETGKISNAQAKQKLPELLTGLSPE
HHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHH
KAFAGQELISDPSVLEPIVDEVIAANPKELEKYRNGNINLKGFFVGQVLKKTNKRADPKL
HHHCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCHHH
TNELVENKLNG
HHHHHHHCCCC
>Mature Secondary Structure 
TSATTVKTEYEAIIGLETHCQLSTNTKIFSSSSTAFGADPNTNIDPVCMGLPGVLPVLN
CCCCEEEEHHHHEEEECCEEEECCCCEEEECCCCCCCCCCCCCCCCEEECCCCHHHHHH
EKVLEYAVKAGLALNCQIAKYSKFDRKQYFYPDLPKNYQISQYDLPIAEHGWLEIELLDA
HHHHHHHHHCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEEECC
DGNPKRKRIGITRLHMEEDAGKLVHAGSDRISGSTYSLVDYNRAGVPLVEIVSEPDIRTG
CCCCCHHHCCEEEEEECCCCCCEEECCCCCCCCCEEEEEECCCCCCCHHEECCCCCCCCC
QEAAEYAQELRRVMRYLGVSDGNMQEGSLRCDVNISVRPVGQEKFGTKVEIKNMNSFSAI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCHHHCCCEEEEECCCHHHHH
QKAIEHEIERQIEAIESGEKIIQETRLWEEGSQRTISMRIKEGSSDYRYFPEPDLAPIEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCEE
TEAQLSQWRSELPELPAQKRHRYENELGLSAYDTRVLTEDVIVSQYFEVAIASGANPKAA
HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
ANWITQDIAAYLNKQKLSITEIGLTPANLADVITRIETGKISNAQAKQKLPELLTGLSPE
HHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHH
KAFAGQELISDPSVLEPIVDEVIAANPKELEKYRNGNINLKGFFVGQVLKKTNKRADPKL
HHHCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCHHH
TNELVENKLNG
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA