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The map label for this gene is purT [H]

Identifier: 17228794

GI number: 17228794

Start: 1540090

End: 1541265

Strand: Direct

Name: purT [H]

Synonym: alr1299

Alternate gene names: 17228794

Gene position: 1540090-1541265 (Clockwise)

Preceding gene: 17228793

Following gene: 17228795

Centisome position: 24.01

GC content: 42.69

Gene sequence:

>1176_bases
ATGAGTAAGGCAATTAAGCTACCCCAAAAACTAATGTTGTTGGGGTCAGGTGAGTTGGGCAAAGAATTTGTCATTGCGGC
TCAACGTCTTGGTAATTATGTGATAGCCGTTGACCGCTATGCCAATGCACCCGCGATGCAGGTTTCCGATTGTTGTGAAG
TCATATCAATGCTTAGTGCTGATGATTTAGAAGCTGTAGTCACTAAGTATGAACCTGATTTTATCATACCAGAGATTGAA
GCAATTAGAACAGAGAAACTACAGGAATTTGAAGACAGAGGTATCACTGTAATCCCCACTGCGGCAGCTACCAATTATAC
AATGAACCGCGATCGCATCCGGGAATTAGCCCATGAAGAATTAGGTATTAGAACAGCGAAATACGGCTATGCTGTCACCT
TAGAAGAATTGATTGCTGTTTCTGATAATATAGGGTTTCCTAATGTAGTCAAACCTGTGATGTCGTCCTCAGGTAAAGGA
CAATCGGTAGTTGCCACAAAAGAGGAAGTAGAGAAAGCTTGGGATTATGCGATCGCTAATTCTCGTGGTGATAGTCAAAA
AGTCATCGTCGAAGAATTTATCAACTTTGAAATTGAAATCACCCTCCTCACCATTAAACAGTGGAATGCACCCACAATTT
TCTGTTCACCCATCGGACACCGCCAAGAACGAGGCGATTATCAAGAATCTTGGCAACCGGCTGGTATTAGCGAAGATAAG
ATATTAGAAGCGCAAGCCATAGCCAAAACCGTCACCGATGCTTTAGGCGGTGCTGGCATATTTGGGGTAGAGTTTTTTAT
AACTAAAGACGAAGTAATTTTTTCTGAACTATCACCCCGTCCCCACGATACAGGCATGGTGACATTAATTTCACAAAACT
TGAACGAATTTGAGTTACATCTTAGGGCAATTCTCGGCTTACCAATTCCTAAAATAGAACAGTTAGGTTTCTGCGCCAGT
GCAGTAATTTTAGCCTCAGAAAAATTGGATGCACCCCTGTTTACAGGTGTCGCCGAAGCCTTAGCCGAACCAGATGTAGA
CATCAGACTATTTGGTAAACCTACAGCACATCCTTATCGTCGTATGGGTGTAGCTTTAGCCAAAGCCGAGAATGTGGAAA
CAGCTAGGGAGAAAGCGACAGATGCCGCGAGTAAAATTCACATAAGTAGTCAATAA

Upstream 100 bases:

>100_bases
TTACAACTGCGGGGAGCTAAGTTCAACTATTTGGCAGATGATAATTAAAATGCAGTATTAATTATTAGGCTGCTAGATTT
TGATTATCTAGGAAAGGTGC

Downstream 100 bases:

>100_bases
ATTGCTGACTACTGCGAAAAAACTCCCAAAGTTCCTAAAAATGCCCATATTTCAGAGGACAAATTTAGTTATGCAGGCAT
TATCCGATGAGACTATAATT

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSKAIKLPQKLMLLGSGELGKEFVIAAQRLGNYVIAVDRYANAPAMQVSDCCEVISMLSADDLEAVVTKYEPDFIIPEIE
AIRTEKLQEFEDRGITVIPTAAATNYTMNRDRIRELAHEELGIRTAKYGYAVTLEELIAVSDNIGFPNVVKPVMSSSGKG
QSVVATKEEVEKAWDYAIANSRGDSQKVIVEEFINFEIEITLLTIKQWNAPTIFCSPIGHRQERGDYQESWQPAGISEDK
ILEAQAIAKTVTDALGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELHLRAILGLPIPKIEQLGFCAS
AVILASEKLDAPLFTGVAEALAEPDVDIRLFGKPTAHPYRRMGVALAKAENVETAREKATDAASKIHISSQ

Sequences:

>Translated_391_residues
MSKAIKLPQKLMLLGSGELGKEFVIAAQRLGNYVIAVDRYANAPAMQVSDCCEVISMLSADDLEAVVTKYEPDFIIPEIE
AIRTEKLQEFEDRGITVIPTAAATNYTMNRDRIRELAHEELGIRTAKYGYAVTLEELIAVSDNIGFPNVVKPVMSSSGKG
QSVVATKEEVEKAWDYAIANSRGDSQKVIVEEFINFEIEITLLTIKQWNAPTIFCSPIGHRQERGDYQESWQPAGISEDK
ILEAQAIAKTVTDALGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELHLRAILGLPIPKIEQLGFCAS
AVILASEKLDAPLFTGVAEALAEPDVDIRLFGKPTAHPYRRMGVALAKAENVETAREKATDAASKIHISSQ
>Mature_390_residues
SKAIKLPQKLMLLGSGELGKEFVIAAQRLGNYVIAVDRYANAPAMQVSDCCEVISMLSADDLEAVVTKYEPDFIIPEIEA
IRTEKLQEFEDRGITVIPTAAATNYTMNRDRIRELAHEELGIRTAKYGYAVTLEELIAVSDNIGFPNVVKPVMSSSGKGQ
SVVATKEEVEKAWDYAIANSRGDSQKVIVEEFINFEIEITLLTIKQWNAPTIFCSPIGHRQERGDYQESWQPAGISEDKI
LEAQAIAKTVTDALGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELHLRAILGLPIPKIEQLGFCASA
VILASEKLDAPLFTGVAEALAEPDVDIRLFGKPTAHPYRRMGVALAKAENVETAREKATDAASKIHISSQ

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=381, Percent_Identity=53.8057742782152, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1786733, Length=195, Percent_Identity=28.2051282051282, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324702, Length=375, Percent_Identity=26.4, Blast_Score=109, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42898; Mature: 42767

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAIKLPQKLMLLGSGELGKEFVIAAQRLGNYVIAVDRYANAPAMQVSDCCEVISMLSA
CCCCHHCCHHHEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHCC
DDLEAVVTKYEPDFIIPEIEAIRTEKLQEFEDRGITVIPTAAATNYTMNRDRIRELAHEE
CHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHH
LGIRTAKYGYAVTLEELIAVSDNIGFPNVVKPVMSSSGKGQSVVATKEEVEKAWDYAIAN
HCCEEECCCEEEEHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHC
SRGDSQKVIVEEFINFEIEITLLTIKQWNAPTIFCSPIGHRQERGDYQESWQPAGISEDK
CCCCCHHHHHHHHCCEEEEEEEEEEECCCCCEEEECCCCCCHHCCCHHHCCCCCCCCHHH
ILEAQAIAKTVTDALGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELH
HHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHCCCCCCCCCEEEEECCCCCHHHHH
LRAILGLPIPKIEQLGFCASAVILASEKLDAPLFTGVAEALAEPDVDIRLFGKPTAHPYR
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHH
RMGVALAKAENVETAREKATDAASKIHISSQ
HHHHHHHHHCCHHHHHHHHHHHHHEEEECCC
>Mature Secondary Structure 
SKAIKLPQKLMLLGSGELGKEFVIAAQRLGNYVIAVDRYANAPAMQVSDCCEVISMLSA
CCCHHCCHHHEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHCC
DDLEAVVTKYEPDFIIPEIEAIRTEKLQEFEDRGITVIPTAAATNYTMNRDRIRELAHEE
CHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHH
LGIRTAKYGYAVTLEELIAVSDNIGFPNVVKPVMSSSGKGQSVVATKEEVEKAWDYAIAN
HCCEEECCCEEEEHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHC
SRGDSQKVIVEEFINFEIEITLLTIKQWNAPTIFCSPIGHRQERGDYQESWQPAGISEDK
CCCCCHHHHHHHHCCEEEEEEEEEEECCCCCEEEECCCCCCHHCCCHHHCCCCCCCCHHH
ILEAQAIAKTVTDALGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELH
HHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHCCCCCCCCCEEEEECCCCCHHHHH
LRAILGLPIPKIEQLGFCASAVILASEKLDAPLFTGVAEALAEPDVDIRLFGKPTAHPYR
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHH
RMGVALAKAENVETAREKATDAASKIHISSQ
HHHHHHHHHCCHHHHHHHHHHHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA