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The map label for this gene is dnaK [H]

Identifier: 17228759

GI number: 17228759

Start: 1499374

End: 1500972

Strand: Direct

Name: dnaK [H]

Synonym: alr1264

Alternate gene names: 17228759

Gene position: 1499374-1500972 (Clockwise)

Preceding gene: 17228758

Following gene: 17228761

Centisome position: 23.38

GC content: 43.28

Gene sequence:

>1599_bases
ATGGCGATCGCGATTGACTTTGGTACAAGTAACACCGTAATTGCTCGTTGGAACCCCGTCACCCAACAACCAGAAACCAT
CACCTTACCAGGCTTATCCATCCAGCAAAGCCTCAATCCCCCACTAATTCCCAGCTTGGTTTACGTAGAAGATGCCAACC
AAAGTAAAATCTTAGTAGGGCAACAAGTACGCGATCGCGGTCTTGACCTCAAAGGTGATACCCGATTCTTTCGCAGCTTC
AAACGAGGAATTGGGGCAGATATCCAAGGCTTCTTACCAGAACTAGACGGGCAAATTGTCACCTTTGAACAAGTCGGACA
ATGGTTCCTCACCGAAGTTATTCAACAACTAGCACCCCTAGAAGGCGGTTTAGATTCCCTAGTATTAACAGTACCTGTAG
ATAGCTTTGAAGCTTATCGTTATTGGTTAGGTCAAGTCTGCCAAGCCCTACCTGTGGAACAAGTGCGGATGTTAGATGAA
CCCACAGCCGCCGCCTTGGGTTATGGTTTAGCAGACCAAGAAATTCTCTTAGTAGTTGATTTTGGTGGCGGGACATTAGA
TTTATCCCTCGTCCAGTTAAATAAAAGCGCCCAAACAAATACAAAACCTGTAGGATTCTTACTCAAATGGGGTAATAAAT
CCCTAGCAGAAGAATCTAAACAAAAAACCAAAACTGCCCGTGTATTGGCGAAAGCCGGGCAGAATTTAGGCGGTACAGAT
ATTGATAATTGGTTAGTAGATTACTTTGCCAAAACTCAAGGGCTAGGGATAAGCCCTTTAACAACGCGACTAGCAGAAAG
GATAAAAATTCAACTATCAGCCCAAAACCAAGCCAGTGAAGTTTATTTTGATGATGAAAACTTTGAAAGTTACGAATTGG
AATTAAATCGGGATACTTTAGAAAGCATCCTCAAAGAACATGGGTTTTTTGAGCAGTTAGATGATGCAATGGCTAATTTA
TTACAACAAGCACGCCGCCAAGGAATCGAACTAGCAGATATTAATGCAGTGTTATTAGTTGGTGGTACAGTACAACTACC
CGCAGTGCAGACATGGGTAAAGCAATATTTTCAGCCAGAGAAAATCCGTTGTGAAAAACCATTTGAGGCGATCGCTCAAG
GTGCATTGCAACTATCTCAAGGTGTAGAAATCAAAGACTTTCTCTACCACAGTTATGGTGTGCGCTATTGGGATCGTCGC
AATCAACGCCACAGTTGGCATTCAATTATTAAGGCTGGGCAAGGCTACCCCATGAATCAGCCAGTAGAATTGGTTTTAGG
CGCTTCTTTGGAGAATCAATCAAGTATTGAATTAATCTTGGGTGAACTAGGTACAGAGACGGGGGGAACAGAAGTATATT
TTGATGGCGATCGCTTAATTACCCGCCGCCTAGATACCGGTGTCACCAGCGTTAAACCCCTCAACGATAAAGAAGGCGCA
AGAACTATAGCCCAACTCACACCACCAGGATTTCCGGGGAGCGATCGTATTAAAATCTTGTTTCAAGTTGATGAACAGCG
CTTTTTACGCATCACCGTTGAAGATTTATTGACTAATGATACTTTGCTAGAAAATCAACTGGTGGCACAGTTGAGTTAA

Upstream 100 bases:

>100_bases
CGATCGCCCACTTGCGCTCATGGTGGCTTATGGGACAATAGTTCTTTGATATAAAAGCACTTTGTGATTTTGCGTTTTTG
CGCGAACATAATAAAAGAAT

Downstream 100 bases:

>100_bases
ATTAGTTTGGGGTGGAATGGATTTAAGTTTACGGGTTTTATCTATAATTGAGTGCGATCGCCTAAATCTTCAACATGAAA
AAGACAGCAGTTTTTCCCCC

Product: DnaK-type molecular chaperone

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MAIAIDFGTSNTVIARWNPVTQQPETITLPGLSIQQSLNPPLIPSLVYVEDANQSKILVGQQVRDRGLDLKGDTRFFRSF
KRGIGADIQGFLPELDGQIVTFEQVGQWFLTEVIQQLAPLEGGLDSLVLTVPVDSFEAYRYWLGQVCQALPVEQVRMLDE
PTAAALGYGLADQEILLVVDFGGGTLDLSLVQLNKSAQTNTKPVGFLLKWGNKSLAEESKQKTKTARVLAKAGQNLGGTD
IDNWLVDYFAKTQGLGISPLTTRLAERIKIQLSAQNQASEVYFDDENFESYELELNRDTLESILKEHGFFEQLDDAMANL
LQQARRQGIELADINAVLLVGGTVQLPAVQTWVKQYFQPEKIRCEKPFEAIAQGALQLSQGVEIKDFLYHSYGVRYWDRR
NQRHSWHSIIKAGQGYPMNQPVELVLGASLENQSSIELILGELGTETGGTEVYFDGDRLITRRLDTGVTSVKPLNDKEGA
RTIAQLTPPGFPGSDRIKILFQVDEQRFLRITVEDLLTNDTLLENQLVAQLS

Sequences:

>Translated_532_residues
MAIAIDFGTSNTVIARWNPVTQQPETITLPGLSIQQSLNPPLIPSLVYVEDANQSKILVGQQVRDRGLDLKGDTRFFRSF
KRGIGADIQGFLPELDGQIVTFEQVGQWFLTEVIQQLAPLEGGLDSLVLTVPVDSFEAYRYWLGQVCQALPVEQVRMLDE
PTAAALGYGLADQEILLVVDFGGGTLDLSLVQLNKSAQTNTKPVGFLLKWGNKSLAEESKQKTKTARVLAKAGQNLGGTD
IDNWLVDYFAKTQGLGISPLTTRLAERIKIQLSAQNQASEVYFDDENFESYELELNRDTLESILKEHGFFEQLDDAMANL
LQQARRQGIELADINAVLLVGGTVQLPAVQTWVKQYFQPEKIRCEKPFEAIAQGALQLSQGVEIKDFLYHSYGVRYWDRR
NQRHSWHSIIKAGQGYPMNQPVELVLGASLENQSSIELILGELGTETGGTEVYFDGDRLITRRLDTGVTSVKPLNDKEGA
RTIAQLTPPGFPGSDRIKILFQVDEQRFLRITVEDLLTNDTLLENQLVAQLS
>Mature_531_residues
AIAIDFGTSNTVIARWNPVTQQPETITLPGLSIQQSLNPPLIPSLVYVEDANQSKILVGQQVRDRGLDLKGDTRFFRSFK
RGIGADIQGFLPELDGQIVTFEQVGQWFLTEVIQQLAPLEGGLDSLVLTVPVDSFEAYRYWLGQVCQALPVEQVRMLDEP
TAAALGYGLADQEILLVVDFGGGTLDLSLVQLNKSAQTNTKPVGFLLKWGNKSLAEESKQKTKTARVLAKAGQNLGGTDI
DNWLVDYFAKTQGLGISPLTTRLAERIKIQLSAQNQASEVYFDDENFESYELELNRDTLESILKEHGFFEQLDDAMANLL
QQARRQGIELADINAVLLVGGTVQLPAVQTWVKQYFQPEKIRCEKPFEAIAQGALQLSQGVEIKDFLYHSYGVRYWDRRN
QRHSWHSIIKAGQGYPMNQPVELVLGASLENQSSIELILGELGTETGGTEVYFDGDRLITRRLDTGVTSVKPLNDKEGAR
TIAQLTPPGFPGSDRIKILFQVDEQRFLRITVEDLLTNDTLLENQLVAQLS

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Escherichia coli, GI1786196, Length=430, Percent_Identity=27.2093023255814, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788875, Length=412, Percent_Identity=26.2135922330097, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1786870, Length=389, Percent_Identity=25.4498714652956, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17534771, Length=319, Percent_Identity=26.9592476489028, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17568549, Length=319, Percent_Identity=26.3322884012539, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI32564617, Length=429, Percent_Identity=23.3100233100233, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17737941, Length=303, Percent_Identity=27.7227722772277, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 59213; Mature: 59082

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIAIDFGTSNTVIARWNPVTQQPETITLPGLSIQQSLNPPLIPSLVYVEDANQSKILVG
CEEEEEECCCCEEEEECCCCCCCCCEEEECCCCHHHCCCCCCCCEEEEEECCCCCEEEEC
QQVRDRGLDLKGDTRFFRSFKRGIGADIQGFLPELDGQIVTFEQVGQWFLTEVIQQLAPL
HHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCC
EGGLDSLVLTVPVDSFEAYRYWLGQVCQALPVEQVRMLDEPTAAALGYGLADQEILLVVD
CCCCCCEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHCCCCCCCEEEEEEE
FGGGTLDLSLVQLNKSAQTNTKPVGFLLKWGNKSLAEESKQKTKTARVLAKAGQNLGGTD
CCCCEEEEEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
IDNWLVDYFAKTQGLGISPLTTRLAERIKIQLSAQNQASEVYFDDENFESYELELNRDTL
HHHHHHHHHHHHCCCCCCHHHHHHHHHEEEEEECCCCCCEEEECCCCCCEEEEEECHHHH
ESILKEHGFFEQLDDAMANLLQQARRQGIELADINAVLLVGGTVQLPAVQTWVKQYFQPE
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCEECHHHHHHHHHHCCCC
KIRCEKPFEAIAQGALQLSQGVEIKDFLYHSYGVRYWDRRNQRHSWHSIIKAGQGYPMNQ
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHCCCCCHHHHHHHHCCCCCCCCC
PVELVLGASLENQSSIELILGELGTETGGTEVYFDGDRLITRRLDTGVTSVKPLNDKEGA
CHHEEEECCCCCCCCHHHHEEHHCCCCCCEEEEECCCHHEEHHHHCCCCCCCCCCCCHHH
RTIAQLTPPGFPGSDRIKILFQVDEQRFLRITVEDLLTNDTLLENQLVAQLS
HHHHHCCCCCCCCCCCEEEEEEECCCEEEEEEHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AIAIDFGTSNTVIARWNPVTQQPETITLPGLSIQQSLNPPLIPSLVYVEDANQSKILVG
EEEEEECCCCEEEEECCCCCCCCCEEEECCCCHHHCCCCCCCCEEEEEECCCCCEEEEC
QQVRDRGLDLKGDTRFFRSFKRGIGADIQGFLPELDGQIVTFEQVGQWFLTEVIQQLAPL
HHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCC
EGGLDSLVLTVPVDSFEAYRYWLGQVCQALPVEQVRMLDEPTAAALGYGLADQEILLVVD
CCCCCCEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHCCCCCCCEEEEEEE
FGGGTLDLSLVQLNKSAQTNTKPVGFLLKWGNKSLAEESKQKTKTARVLAKAGQNLGGTD
CCCCEEEEEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
IDNWLVDYFAKTQGLGISPLTTRLAERIKIQLSAQNQASEVYFDDENFESYELELNRDTL
HHHHHHHHHHHHCCCCCCHHHHHHHHHEEEEEECCCCCCEEEECCCCCCEEEEEECHHHH
ESILKEHGFFEQLDDAMANLLQQARRQGIELADINAVLLVGGTVQLPAVQTWVKQYFQPE
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCEECHHHHHHHHHHCCCC
KIRCEKPFEAIAQGALQLSQGVEIKDFLYHSYGVRYWDRRNQRHSWHSIIKAGQGYPMNQ
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHCCCCCHHHHHHHHCCCCCCCCC
PVELVLGASLENQSSIELILGELGTETGGTEVYFDGDRLITRRLDTGVTSVKPLNDKEGA
CHHEEEECCCCCCCCHHHHEEHHCCCCCCEEEEECCCHHEEHHHHCCCCCCCCCCCCHHH
RTIAQLTPPGFPGSDRIKILFQVDEQRFLRITVEDLLTNDTLLENQLVAQLS
HHHHHCCCCCCCCCCCEEEEEEECCCEEEEEEHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA