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The map label for this gene is carA

Identifier: 17228650

GI number: 17228650

Start: 1360455

End: 1361621

Strand: Direct

Name: carA

Synonym: alr1155

Alternate gene names: 17228650

Gene position: 1360455-1361621 (Clockwise)

Preceding gene: 17228648

Following gene: 17228651

Centisome position: 21.21

GC content: 48.24

Gene sequence:

>1167_bases
ATGCCCCTGTCTGACGCAATGCCTGCTCTCCTTGTCTTGGCAGATGGAACTGCTTACCGTGGTTGGTCTTTCGGTGCGAC
GGGAACCACCATTGGCGAAGTCGTCTTCAACACTGGTATGACTGGCTATCAAGAAGTATTGACAGATCCTAGTTACCGTG
GTCAAATTGTCGTCTTCACCTATCCCGAATTGGGGAATACAGGCGTTAATCCTGAAGACGAAGAATCAGCACGTCCCCAA
GTGCGGGGAGCGATCGCCCGCAATATTTGTCATAAACCGAGTAACTGGCGATCCACACAATCCTTACCCGACTACCTCAA
ACAGCACCACATCCCCGGTATCTTTGGTATAGATACCCGCGCCCTCACCCGCAAAATTCGGATGTACGGAGCCATGAACG
GCGGTATTTCTACAGAAATTTTGGATGAAGCGGAGTTGTTAGAACAGGTACAAGCAGCTCCTAACATGGCAGGCTTAAAC
TTAGTCCGGGAAGTTACCACCTCTACAGTCTACGAATGGTCAGACCCTACAACAGCAGTTTGGGAATTTAACCCAGATGG
TACGGTAAAGAATGGGGAATCCTTGACAGTTGTCGCCCTAGATTTTGGTGTTAAACGTAATATTTTACGTCGTTTAGCCA
GTTATGGTTGCCGAGTCATTGTTGTACCTGCCGATACATCACCAGAAGAAATCCTCAAATACAATCCAGATGGTATTTTC
CTCTCGAACGGCCCTGGCGACCCGTCGGCAGTTACAGAAGGTATTACCACAGCCAAAGCATTGCTCGAAAGCGAAAAGCC
CATTTTCGGTATTTGTATGGGACACCAAATTTTGGGTCACGCCTTGGGAGCAGAAACCTTTAAGCTGAAATTTGGACATC
GGGGTTTAAATCAACCAGCCGGTTTAACACAACGGGTAGAAATCACCAGCCAAAACCACAGCTTTGCGATCGACCCAGAT
TCTTTACCTGAGGCAGTTGTGGAAATCAGCCACCTCAATTTAAACGATCGCACTGTTGCAGGTCTGCGCCACAAATCTCT
ACCCATCTTTTCCGTGCAGTACCACCCAGAAGCCAGCCCAGGCCCTCACGATGCTGATTACTTGTTTGCCCAGTTTGTTC
ATCAAATGCGGACAACAAAACAAGCCACTACAGCAGAAGTGAGTTAA

Upstream 100 bases:

>100_bases
GAAAAAATCTCAACAATCACAGAGCAACGGAGAAAAAAATTTCTCCAATTCCCAGTCCCCCATCCCCAATCCCCCCAGTC
CCCATCCTAATTCATCGCTT

Downstream 100 bases:

>100_bases
AAAGGGATTGGGGAATAGGAACTAGGAACTAGAAAGGTACAGGGAATAAGGGAGAAGAAATTTACCTAACACCCAATACC
CAGTCCCCGATCCCCATACC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MPLSDAMPALLVLADGTAYRGWSFGATGTTIGEVVFNTGMTGYQEVLTDPSYRGQIVVFTYPELGNTGVNPEDEESARPQ
VRGAIARNICHKPSNWRSTQSLPDYLKQHHIPGIFGIDTRALTRKIRMYGAMNGGISTEILDEAELLEQVQAAPNMAGLN
LVREVTTSTVYEWSDPTTAVWEFNPDGTVKNGESLTVVALDFGVKRNILRRLASYGCRVIVVPADTSPEEILKYNPDGIF
LSNGPGDPSAVTEGITTAKALLESEKPIFGICMGHQILGHALGAETFKLKFGHRGLNQPAGLTQRVEITSQNHSFAIDPD
SLPEAVVEISHLNLNDRTVAGLRHKSLPIFSVQYHPEASPGPHDADYLFAQFVHQMRTTKQATTAEVS

Sequences:

>Translated_388_residues
MPLSDAMPALLVLADGTAYRGWSFGATGTTIGEVVFNTGMTGYQEVLTDPSYRGQIVVFTYPELGNTGVNPEDEESARPQ
VRGAIARNICHKPSNWRSTQSLPDYLKQHHIPGIFGIDTRALTRKIRMYGAMNGGISTEILDEAELLEQVQAAPNMAGLN
LVREVTTSTVYEWSDPTTAVWEFNPDGTVKNGESLTVVALDFGVKRNILRRLASYGCRVIVVPADTSPEEILKYNPDGIF
LSNGPGDPSAVTEGITTAKALLESEKPIFGICMGHQILGHALGAETFKLKFGHRGLNQPAGLTQRVEITSQNHSFAIDPD
SLPEAVVEISHLNLNDRTVAGLRHKSLPIFSVQYHPEASPGPHDADYLFAQFVHQMRTTKQATTAEVS
>Mature_387_residues
PLSDAMPALLVLADGTAYRGWSFGATGTTIGEVVFNTGMTGYQEVLTDPSYRGQIVVFTYPELGNTGVNPEDEESARPQV
RGAIARNICHKPSNWRSTQSLPDYLKQHHIPGIFGIDTRALTRKIRMYGAMNGGISTEILDEAELLEQVQAAPNMAGLNL
VREVTTSTVYEWSDPTTAVWEFNPDGTVKNGESLTVVALDFGVKRNILRRLASYGCRVIVVPADTSPEEILKYNPDGIFL
SNGPGDPSAVTEGITTAKALLESEKPIFGICMGHQILGHALGAETFKLKFGHRGLNQPAGLTQRVEITSQNHSFAIDPDS
LPEAVVEISHLNLNDRTVAGLRHKSLPIFSVQYHPEASPGPHDADYLFAQFVHQMRTTKQATTAEVS

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=388, Percent_Identity=41.4948453608247, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI169790915, Length=398, Percent_Identity=37.1859296482412, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI21361331, Length=396, Percent_Identity=36.8686868686869, Blast_Score=231, Evalue=9e-61,
Organism=Escherichia coli, GI1786215, Length=379, Percent_Identity=49.6042216358839, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI193204318, Length=398, Percent_Identity=41.2060301507538, Blast_Score=263, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6322331, Length=399, Percent_Identity=38.0952380952381, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6324878, Length=401, Percent_Identity=36.6583541147132, Blast_Score=236, Evalue=4e-63,
Organism=Drosophila melanogaster, GI45555749, Length=388, Percent_Identity=38.659793814433, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24642586, Length=388, Percent_Identity=38.659793814433, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_NOSS1 (Q8YXQ7)

Other databases:

- EMBL:   BA000019
- PIR:   AH1950
- RefSeq:   NP_485198.1
- ProteinModelPortal:   Q8YXQ7
- SMR:   Q8YXQ7
- STRING:   Q8YXQ7
- GeneID:   1104749
- GenomeReviews:   BA000019_GR
- KEGG:   ana:alr1155
- NMPDR:   fig|103690.1.peg.1465
- eggNOG:   COG0505
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- PhylomeDB:   Q8YXQ7
- ProtClustDB:   PRK12564
- BioCyc:   NSP103690:ALR1155-MONOMER
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 42257; Mature: 42126

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 272-272 ACT_SITE 355-355 ACT_SITE 357-357

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSDAMPALLVLADGTAYRGWSFGATGTTIGEVVFNTGMTGYQEVLTDPSYRGQIVVFT
CCCCCCCCEEEEEECCCEECCEECCCCCCHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEE
YPELGNTGVNPEDEESARPQVRGAIARNICHKPSNWRSTQSLPDYLKQHHIPGIFGIDTR
CCCCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEECCHH
ALTRKIRMYGAMNGGISTEILDEAELLEQVQAAPNMAGLNLVREVTTSTVYEWSDPTTAV
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCEEE
WEFNPDGTVKNGESLTVVALDFGVKRNILRRLASYGCRVIVVPADTSPEEILKYNPDGIF
EEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHEECCCEEE
LSNGPGDPSAVTEGITTAKALLESEKPIFGICMGHQILGHALGAETFKLKFGHRGLNQPA
EECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCEEEEEECCCCCCCCC
GLTQRVEITSQNHSFAIDPDSLPEAVVEISHLNLNDRTVAGLRHKSLPIFSVQYHPEASP
CCCEEEEEECCCCCEEECCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEEECCCCCC
GPHDADYLFAQFVHQMRTTKQATTAEVS
CCCCHHHHHHHHHHHHHHHHHCHHCCCC
>Mature Secondary Structure 
PLSDAMPALLVLADGTAYRGWSFGATGTTIGEVVFNTGMTGYQEVLTDPSYRGQIVVFT
CCCCCCCEEEEEECCCEECCEECCCCCCHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEE
YPELGNTGVNPEDEESARPQVRGAIARNICHKPSNWRSTQSLPDYLKQHHIPGIFGIDTR
CCCCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEECCHH
ALTRKIRMYGAMNGGISTEILDEAELLEQVQAAPNMAGLNLVREVTTSTVYEWSDPTTAV
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCEEE
WEFNPDGTVKNGESLTVVALDFGVKRNILRRLASYGCRVIVVPADTSPEEILKYNPDGIF
EEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHEECCCEEE
LSNGPGDPSAVTEGITTAKALLESEKPIFGICMGHQILGHALGAETFKLKFGHRGLNQPA
EECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCEEEEEECCCCCCCCC
GLTQRVEITSQNHSFAIDPDSLPEAVVEISHLNLNDRTVAGLRHKSLPIFSVQYHPEASP
CCCEEEEEECCCCCEEECCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEEECCCCCC
GPHDADYLFAQFVHQMRTTKQATTAEVS
CCCCHHHHHHHHHHHHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840