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The map label for this gene is sinIR [H]

Identifier: 17228428

GI number: 17228428

Start: 1085500

End: 1086192

Strand: Direct

Name: sinIR [H]

Synonym: alr0933

Alternate gene names: 17228428

Gene position: 1085500-1086192 (Clockwise)

Preceding gene: 17228426

Following gene: 17228434

Centisome position: 16.92

GC content: 40.84

Gene sequence:

>693_bases
ATGATGTCGAAATTTATACAAAATGCAGCAGAGATTGCAAAAAAGGCGATGGATAGTGTTGATCCATCACTTAGTGAGAA
ATTTACAATTGTTATACGATTCCTTACCGATAACCCTGATGCGGCCTCGGCGCTAAAAGGTAAAGAGCGGTCAATCGTAG
GGACGGAAGAATATATTATCGCCAGTGCAACAAATTTCAAAAAAGGTCGTGACCCAAGAACACCTTTGCCACCTAGTACA
ATCCCGGATGAAATGGTAAGCGTCATCCTGAACAAGTATTTTGAAGTTCCCTCTGAGGAACTGGAAAAAGCCGAAGAATG
GCACCGCCTATCGATGGGGGCTGAGAATATTGTGGGGGATTTGTTAGAAAGGTATATTGCAGAAGTCATCGAGCCGCATG
GATGGATCTGGTGTTCAGGTTCTATGGTGAGAGCAGTTGATTTTATATATTGTGATAGCGAAAATGTCTGGCAATCACTG
CAAGTAAAAAACAGGGATAATACTGAAAATTCTTCTTCGGCCGCTATACGTCATGGCACCCCAATTAAAAAATGGTTCCG
AACTTTTTCTAAAAAAAGGGGAGATAATTGGGATAAATTCCCTTCATTGGAAGGGAAAGAGAATCTTTCAGAAAAAGGCT
TCAAATTGTATGTGGAAAAATACCTTTCTGCCTTAAGAGCTATCAAAGCCTAA

Upstream 100 bases:

>100_bases
AGCGGAAATTTAGTGTAAAACTACAGCAATTCTCATTATGAGAATCTTGGAAGCATATTAATTTATGTTATAAAACTGAA
ACATTTAATGCTTCTAGGAA

Downstream 100 bases:

>100_bases
CTCTTATAAAGCACATACCGTAAAAGGTGAACCCTGGACTGGTTTTTCCTCTTCTAGGGATTTTGTAATTTTAGCCTCCC
ATGACACATCATCGTTACCT

Product: type II site-specific deoxyribonuclease

Products: NA

Alternate protein names: R.SinI; Endonuclease SinI; Type II restriction enzyme SinI [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MMSKFIQNAAEIAKKAMDSVDPSLSEKFTIVIRFLTDNPDAASALKGKERSIVGTEEYIIASATNFKKGRDPRTPLPPST
IPDEMVSVILNKYFEVPSEELEKAEEWHRLSMGAENIVGDLLERYIAEVIEPHGWIWCSGSMVRAVDFIYCDSENVWQSL
QVKNRDNTENSSSAAIRHGTPIKKWFRTFSKKRGDNWDKFPSLEGKENLSEKGFKLYVEKYLSALRAIKA

Sequences:

>Translated_230_residues
MMSKFIQNAAEIAKKAMDSVDPSLSEKFTIVIRFLTDNPDAASALKGKERSIVGTEEYIIASATNFKKGRDPRTPLPPST
IPDEMVSVILNKYFEVPSEELEKAEEWHRLSMGAENIVGDLLERYIAEVIEPHGWIWCSGSMVRAVDFIYCDSENVWQSL
QVKNRDNTENSSSAAIRHGTPIKKWFRTFSKKRGDNWDKFPSLEGKENLSEKGFKLYVEKYLSALRAIKA
>Mature_230_residues
MMSKFIQNAAEIAKKAMDSVDPSLSEKFTIVIRFLTDNPDAASALKGKERSIVGTEEYIIASATNFKKGRDPRTPLPPST
IPDEMVSVILNKYFEVPSEELEKAEEWHRLSMGAENIVGDLLERYIAEVIEPHGWIWCSGSMVRAVDFIYCDSENVWQSL
QVKNRDNTENSSSAAIRHGTPIKKWFRTFSKKRGDNWDKFPSLEGKENLSEKGFKLYVEKYLSALRAIKA

Specific function: Recognizes the double-stranded sequence GGWCC and cleaves after G-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019070 [H]

Pfam domain/function: PF09570 RE_SinI [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 26129; Mature: 26129

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSKFIQNAAEIAKKAMDSVDPSLSEKFTIVIRFLTDNPDAASALKGKERSIVGTEEYII
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHCCCCCCCCCCHHEEE
ASATNFKKGRDPRTPLPPSTIPDEMVSVILNKYFEVPSEELEKAEEWHRLSMGAENIVGD
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHH
LLERYIAEVIEPHGWIWCSGSMVRAVDFIYCDSENVWQSLQVKNRDNTENSSSAAIRHGT
HHHHHHHHHHCCCCEEEECCCHHHHHHEEEECCHHHHHHHHHCCCCCCCCCCCCHHHCCC
PIKKWFRTFSKKRGDNWDKFPSLEGKENLSEKGFKLYVEKYLSALRAIKA
CHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMSKFIQNAAEIAKKAMDSVDPSLSEKFTIVIRFLTDNPDAASALKGKERSIVGTEEYII
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHCCCCCCCCCCHHEEE
ASATNFKKGRDPRTPLPPSTIPDEMVSVILNKYFEVPSEELEKAEEWHRLSMGAENIVGD
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHH
LLERYIAEVIEPHGWIWCSGSMVRAVDFIYCDSENVWQSLQVKNRDNTENSSSAAIRHGT
HHHHHHHHHHCCCCEEEECCCHHHHHHEEEECCHHHHHHHHHCCCCCCCCCCCCHHHCCC
PIKKWFRTFSKKRGDNWDKFPSLEGKENLSEKGFKLYVEKYLSALRAIKA
CHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2836359 [H]