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The map label for this gene is liaR [H]

Identifier: 17228408

GI number: 17228408

Start: 1056904

End: 1057581

Strand: Direct

Name: liaR [H]

Synonym: alr0913

Alternate gene names: 17228408

Gene position: 1056904-1057581 (Clockwise)

Preceding gene: 17228407

Following gene: 17228415

Centisome position: 16.48

GC content: 46.31

Gene sequence:

>678_bases
ATGTCTCCCAATACCGGCTATTTTCTTAATCTGCCAAGTAATGCTCCCCGGTTGCGAATTTTGATTGTGGAAGATGATCC
AATGATGCAACTGGGGTTGGAACAGTCGTTAATGGCTCATCCCCAATTAGAGATTGTAGGACAAGCAGAAGACGGCTATT
TAGGTGTACAAGCAGCACTAGCACTGAAACCGGATTTGGTAGTCATGGATATTGGCTTACCACGAATTGATGGGATTGCA
GCGACACAGCAAATCAAAGCCGCACTACCGGAAACTCATGTGGTGATGTTGACATCCCATAAAACAGAAACAGAAATTAT
TGCCGCACTTTCAAGCGGTGCAGATGCTTATTGTATCAAAGGTGCAAGCATCGAGCGATTGTTGAATGCGATCGCCGCCG
CCGTCGATGGTGCAACCTACCTCGATCCCCAGATTGCGCGACAAGTAATTGATCATCTCAAACCCCCCTCAGCAAAGGGA
AATACCGCCAATCTATCTGGACGGGAGTTAGAAGTATTAAAACTCATGGTAGAAGGACTGAGTAACCCAGAGATAGCCGA
GAAACTTTATCTCAGTCCTAATACTGTGAAAACTCACGTCCGGGGAATTATGAATAAATTAGCGGTTGACGATCGCGTCC
AAGCGGCTGTTGTGGCGCTACGTTCTGGTTTAGTATAG

Upstream 100 bases:

>100_bases
CCTCCGCTCAGACTTTGCGCTTAGTCAACATTCAAAACAGTTTTGGACTCTGAACTATAGACTATGGACTATGGACTATT
AACTACTGACGAAATAAAAA

Downstream 100 bases:

>100_bases
TTGGCGTAGCATCAGCAACAACGACTGCTCCTGGTTGGATCTGACCGATGGGCGCTGGCTGCATATCGCGGTAATCTAAT
ATTTGGACAACGATTAGATA

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MSPNTGYFLNLPSNAPRLRILIVEDDPMMQLGLEQSLMAHPQLEIVGQAEDGYLGVQAALALKPDLVVMDIGLPRIDGIA
ATQQIKAALPETHVVMLTSHKTETEIIAALSSGADAYCIKGASIERLLNAIAAAVDGATYLDPQIARQVIDHLKPPSAKG
NTANLSGRELEVLKLMVEGLSNPEIAEKLYLSPNTVKTHVRGIMNKLAVDDRVQAAVVALRSGLV

Sequences:

>Translated_225_residues
MSPNTGYFLNLPSNAPRLRILIVEDDPMMQLGLEQSLMAHPQLEIVGQAEDGYLGVQAALALKPDLVVMDIGLPRIDGIA
ATQQIKAALPETHVVMLTSHKTETEIIAALSSGADAYCIKGASIERLLNAIAAAVDGATYLDPQIARQVIDHLKPPSAKG
NTANLSGRELEVLKLMVEGLSNPEIAEKLYLSPNTVKTHVRGIMNKLAVDDRVQAAVVALRSGLV
>Mature_224_residues
SPNTGYFLNLPSNAPRLRILIVEDDPMMQLGLEQSLMAHPQLEIVGQAEDGYLGVQAALALKPDLVVMDIGLPRIDGIAA
TQQIKAALPETHVVMLTSHKTETEIIAALSSGADAYCIKGASIERLLNAIAAAVDGATYLDPQIARQVIDHLKPPSAKGN
TANLSGRELEVLKLMVEGLSNPEIAEKLYLSPNTVKTHVRGIMNKLAVDDRVQAAVVALRSGLV

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=211, Percent_Identity=29.3838862559242, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=30.4761904761905, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786747, Length=198, Percent_Identity=27.7777777777778, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787473, Length=207, Percent_Identity=30.9178743961353, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788712, Length=204, Percent_Identity=24.0196078431373, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790102, Length=197, Percent_Identity=27.4111675126904, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788546, Length=200, Percent_Identity=27, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24029; Mature: 23898

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPNTGYFLNLPSNAPRLRILIVEDDPMMQLGLEQSLMAHPQLEIVGQAEDGYLGVQAAL
CCCCCCEEEECCCCCCEEEEEEECCCCHHHHCCHHHHHCCCCEEEEECCCCCEEEEEEEE
ALKPDLVVMDIGLPRIDGIAATQQIKAALPETHVVMLTSHKTETEIIAALSSGADAYCIK
EECCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEC
GASIERLLNAIAAAVDGATYLDPQIARQVIDHLKPPSAKGNTANLSGRELEVLKLMVEGL
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC
SNPEIAEKLYLSPNTVKTHVRGIMNKLAVDDRVQAAVVALRSGLV
CCCCHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SPNTGYFLNLPSNAPRLRILIVEDDPMMQLGLEQSLMAHPQLEIVGQAEDGYLGVQAAL
CCCCCEEEECCCCCCEEEEEEECCCCHHHHCCHHHHHCCCCEEEEECCCCCEEEEEEEE
ALKPDLVVMDIGLPRIDGIAATQQIKAALPETHVVMLTSHKTETEIIAALSSGADAYCIK
EECCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEC
GASIERLLNAIAAAVDGATYLDPQIARQVIDHLKPPSAKGNTANLSGRELEVLKLMVEGL
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC
SNPEIAEKLYLSPNTVKTHVRGIMNKLAVDDRVQAAVVALRSGLV
CCCCHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]