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The map label for this gene is ligA

Identifier: 170762219

GI number: 170762219

Start: 159525

End: 161546

Strand: Direct

Name: ligA

Synonym: UPA3_0127

Alternate gene names: 170762219

Gene position: 159525-161546 (Clockwise)

Preceding gene: 170762298

Following gene: 170762155

Centisome position: 21.22

GC content: 26.01

Gene sequence:

>2022_bases
ATGGATAAAATAAAAGCTAAAATTGACGAATTAAAACAAAAACTAGACCAATGAAACTATGAATATTATGTTTTAGATGA
TCCAAGTGTGCCAGACCATGTTTATGATCAAACAATGCGGGAGTTAATCGAACTTGAAAATAATTATCCTCAATTTAAAA
CTAACAATTCACCAAGTGTAAAGGTTGGCGGCTTTGTTTCAGAAAAATTTAATAAAGTAAAACATAAAAGACCAATGTTA
TCACTTTCAAATGCTTTTAATGATGATGATTTAAAAAAATTTGATCAAGATAATCAAAATGCTAGTGTCGATTTAAAAGG
GTACGTAGTTGAACCTAAAATTGATGGTTTAAGCATTTCTATTATTTATAAAAATGCCAAATTACACCAGGCAATTACAA
GAGGAGATGGGATTAATGGCGAAGATGTTACTTCCAATATTTTAACGATTAAAGACATTCCTCACTATATTGATCAAAAA
TATAAAGACTACGAAATTGAAGTTCGTGGTGAAGTCTATATGGCTTTTCATGATTTTTATGAAATGAATGATAATTTAGA
AGAAAGTGATAAAAAATTTGCTAATCCAAGAAATGCTGCTGCTGGAACATTAAGAAGTCTAGATAATTCAATTGTTGCAG
AACGTAAATTAAGTGCTTTTATGTATTATTTGGTTAATGCACAAGAACTAGGAATTAAAACGCATTATGAAAGTATTCAA
TTTTTAAGAGATAATAAATTTAAAGTTAGTGATTTAATTGTTAAAGTCGATACAATTAATGAAGTTATTAATCAAATTGA
TTGTTATACTAAAGTGCGTGATAAACTTTCATATATGATTGATGGTATTGTTATTAAAATTAATAATTTAGAAGTTTATG
ATGAGATTGGTTATACTTCTAAATTTCCTAAATGAGCAATTGCTTATAAATTTCCAGCTAATGTTGTTAGCTCACAATTA
CTAGAAATTATTAACGATGTTGGGCGTACAGGAAAAATCTCATATGTTGCTAAAATTAAACCAATTTTATTAGATGGTAG
TATGGTTGAATACGCTACTTTACATAACTTTGATTTTATTAAAGAAAAAGACATTCGAATTAATGATGAAATTAAAATTT
ATAAAGCAGGGGATGTAATTCCTTATGTTGATGGTGTTGATTTATCAAAACGTTTAGCAAATAGTGTACCTTATGAACCA
ATTATAAATTGCCCTTCATGCCAATCAGTTTTAGTACGTGAAAATGATGAAGTTGACCAACGCTGCTTAAATATTTATGG
ATGTAAAAAAATCAATATTGAAAAAATCGTTTATTTTGTTTCACGTAATTGCATGAATATTGAGGGAATGAGTGATGCCA
TTATTAATAAATTTTATGATGCTAATTTAATTAAAAACATTGCAGATTTATACTATTTGCAAAAACACAAAGAATTTATT
TTAACTAGTGATTTTAAAATTAAAGAAAAAAGTTTTAGTAATTTAATTAATAGTATTAATAATTCAAAAAAATGTTCTTT
AGAATTTTTATTAACAGCTTTTGGAATTCGTCATGTTGGACCAAATTTAGCTAAAAAAATAGCTAAACAATTTAAAACAA
TGACTGCTTTAATGCACGCTAATTTTGATGAATTAACTAATGTTGATGCATGTGGGGAAAAAGCAGCCTTATCATTAATT
AATTGGTTTAATGATGAGCATAATGTTTCTTTAGTTAATCAATTACAACAAGTTGGTGTAAATATGGAATATATTGATGA
TTTTATTTATGATGATAACATCAATATAATTGATGAATATAAAAATAAAACTTTTGTTATTACTGGTTCATTTAGTATTA
GCCGTGATGAAATTAAAACTATTTTAGAGAAATATTATCATGCAAAAGTTAAAAACAGTGTTTCTAAAAAAACAGATTAT
GTTTTAGTTGGCACTGAAGCGGGTACGAAATTAGAAAAAGCTAAATTATTAGGTGTGAAAATTATTGAAAATGAATTTTG
AAAAAAGGATAATAATTTTTAA

Upstream 100 bases:

>100_bases
GTGTAAAAAGCAAATAATAAATATATAATTATGTTTATTTTAATATTAGTTTTTTAATTAAAATTTATACTAATAAAGAT
TTAAAAAGGTTACTTTACGT

Downstream 100 bases:

>100_bases
ATTACTTGAATATTAATAATTTTAAGTTGTTTTTGCTGCTAAATTAAAATTATTGTAATGTTTTCTATGATATAGAAAAT
AATAATTTTGTAATAAATAT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MDKIKAKIDELKQKLDQWNYEYYVLDDPSVPDHVYDQTMRELIELENNYPQFKTNNSPSVKVGGFVSEKFNKVKHKRPML
SLSNAFNDDDLKKFDQDNQNASVDLKGYVVEPKIDGLSISIIYKNAKLHQAITRGDGINGEDVTSNILTIKDIPHYIDQK
YKDYEIEVRGEVYMAFHDFYEMNDNLEESDKKFANPRNAAAGTLRSLDNSIVAERKLSAFMYYLVNAQELGIKTHYESIQ
FLRDNKFKVSDLIVKVDTINEVINQIDCYTKVRDKLSYMIDGIVIKINNLEVYDEIGYTSKFPKWAIAYKFPANVVSSQL
LEIINDVGRTGKISYVAKIKPILLDGSMVEYATLHNFDFIKEKDIRINDEIKIYKAGDVIPYVDGVDLSKRLANSVPYEP
IINCPSCQSVLVRENDEVDQRCLNIYGCKKINIEKIVYFVSRNCMNIEGMSDAIINKFYDANLIKNIADLYYLQKHKEFI
LTSDFKIKEKSFSNLINSINNSKKCSLEFLLTAFGIRHVGPNLAKKIAKQFKTMTALMHANFDELTNVDACGEKAALSLI
NWFNDEHNVSLVNQLQQVGVNMEYIDDFIYDDNINIIDEYKNKTFVITGSFSISRDEIKTILEKYYHAKVKNSVSKKTDY
VLVGTEAGTKLEKAKLLGVKIIENEFWKKDNNF

Sequences:

>Translated_673_residues
MDKIKAKIDELKQKLDQ*NYEYYVLDDPSVPDHVYDQTMRELIELENNYPQFKTNNSPSVKVGGFVSEKFNKVKHKRPML
SLSNAFNDDDLKKFDQDNQNASVDLKGYVVEPKIDGLSISIIYKNAKLHQAITRGDGINGEDVTSNILTIKDIPHYIDQK
YKDYEIEVRGEVYMAFHDFYEMNDNLEESDKKFANPRNAAAGTLRSLDNSIVAERKLSAFMYYLVNAQELGIKTHYESIQ
FLRDNKFKVSDLIVKVDTINEVINQIDCYTKVRDKLSYMIDGIVIKINNLEVYDEIGYTSKFPK*AIAYKFPANVVSSQL
LEIINDVGRTGKISYVAKIKPILLDGSMVEYATLHNFDFIKEKDIRINDEIKIYKAGDVIPYVDGVDLSKRLANSVPYEP
IINCPSCQSVLVRENDEVDQRCLNIYGCKKINIEKIVYFVSRNCMNIEGMSDAIINKFYDANLIKNIADLYYLQKHKEFI
LTSDFKIKEKSFSNLINSINNSKKCSLEFLLTAFGIRHVGPNLAKKIAKQFKTMTALMHANFDELTNVDACGEKAALSLI
NWFNDEHNVSLVNQLQQVGVNMEYIDDFIYDDNINIIDEYKNKTFVITGSFSISRDEIKTILEKYYHAKVKNSVSKKTDY
VLVGTEAGTKLEKAKLLGVKIIENEF*KKDNNF
>Mature_673_residues
MDKIKAKIDELKQKLDQ*NYEYYVLDDPSVPDHVYDQTMRELIELENNYPQFKTNNSPSVKVGGFVSEKFNKVKHKRPML
SLSNAFNDDDLKKFDQDNQNASVDLKGYVVEPKIDGLSISIIYKNAKLHQAITRGDGINGEDVTSNILTIKDIPHYIDQK
YKDYEIEVRGEVYMAFHDFYEMNDNLEESDKKFANPRNAAAGTLRSLDNSIVAERKLSAFMYYLVNAQELGIKTHYESIQ
FLRDNKFKVSDLIVKVDTINEVINQIDCYTKVRDKLSYMIDGIVIKINNLEVYDEIGYTSKFPK*AIAYKFPANVVSSQL
LEIINDVGRTGKISYVAKIKPILLDGSMVEYATLHNFDFIKEKDIRINDEIKIYKAGDVIPYVDGVDLSKRLANSVPYEP
IINCPSCQSVLVRENDEVDQRCLNIYGCKKINIEKIVYFVSRNCMNIEGMSDAIINKFYDANLIKNIADLYYLQKHKEFI
LTSDFKIKEKSFSNLINSINNSKKCSLEFLLTAFGIRHVGPNLAKKIAKQFKTMTALMHANFDELTNVDACGEKAALSLI
NWFNDEHNVSLVNQLQQVGVNMEYIDDFIYDDNINIIDEYKNKTFVITGSFSISRDEIKTILEKYYHAKVKNSVSKKTDY
VLVGTEAGTKLEKAKLLGVKIIENEF*KKDNNF

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=675, Percent_Identity=37.3333333333333, Blast_Score=413, Evalue=1e-116,
Organism=Escherichia coli, GI87082305, Length=414, Percent_Identity=21.7391304347826, Blast_Score=102, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 76839; Mature: 76839

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKIKAKIDELKQKLDQNYEYYVLDDPSVPDHVYDQTMRELIELENNYPQFKTNNSPSVK
CCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
VGGFVSEKFNKVKHKRPMLSLSNAFNDDDLKKFDQDNQNASVDLKGYVVEPKIDGLSISI
ECCHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEEE
IYKNAKLHQAITRGDGINGEDVTSNILTIKDIPHYIDQKYKDYEIEVRGEVYMAFHDFYE
EEECCHHHHHHHCCCCCCCCCHHCCEEEECCCCHHHCCCCCCEEEEEECEEEEEEHHHHH
MNDNLEESDKKFANPRNAAAGTLRSLDNSIVAERKLSAFMYYLVNAQELGIKTHYESIQF
CCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LRDNKFKVSDLIVKVDTINEVINQIDCYTKVRDKLSYMIDGIVIKINNLEVYDEIGYTSK
HHCCCEEEEHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECEEEEHHCCCCCC
FPKAIAYKFPANVVSSQLLEIINDVGRTGKISYVAKIKPILLDGSMVEYATLHNFDFIKE
CCHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEECEEEECCCHHHHHHHCCCCCCCC
KDIRINDEIKIYKAGDVIPYVDGVDLSKRLANSVPYEPIINCPSCQSVLVRENDEVDQRC
CCCEECCCEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHCCCCCHHHHHH
LNIYGCKKINIEKIVYFVSRNCMNIEGMSDAIINKFYDANLIKNIADLYYLQKHKEFILT
HHHHCCCEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
SDFKIKEKSFSNLINSINNSKKCSLEFLLTAFGIRHVGPNLAKKIAKQFKTMTALMHANF
CCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCH
DELTNVDACGEKAALSLINWFNDEHNVSLVNQLQQVGVNMEYIDDFIYDDNINIIDEYKN
HHHCCCHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCEEECCCC
KTFVITGSFSISRDEIKTILEKYYHAKVKNSVSKKTDYVLVGTEAGTKLEKAKLLGVKII
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHH
ENEFKKDNNF
HHHHHCCCCC
>Mature Secondary Structure
MDKIKAKIDELKQKLDQNYEYYVLDDPSVPDHVYDQTMRELIELENNYPQFKTNNSPSVK
CCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
VGGFVSEKFNKVKHKRPMLSLSNAFNDDDLKKFDQDNQNASVDLKGYVVEPKIDGLSISI
ECCHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEEE
IYKNAKLHQAITRGDGINGEDVTSNILTIKDIPHYIDQKYKDYEIEVRGEVYMAFHDFYE
EEECCHHHHHHHCCCCCCCCCHHCCEEEECCCCHHHCCCCCCEEEEEECEEEEEEHHHHH
MNDNLEESDKKFANPRNAAAGTLRSLDNSIVAERKLSAFMYYLVNAQELGIKTHYESIQF
CCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LRDNKFKVSDLIVKVDTINEVINQIDCYTKVRDKLSYMIDGIVIKINNLEVYDEIGYTSK
HHCCCEEEEHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECEEEEHHCCCCCC
FPKAIAYKFPANVVSSQLLEIINDVGRTGKISYVAKIKPILLDGSMVEYATLHNFDFIKE
CCHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEECEEEECCCHHHHHHHCCCCCCCC
KDIRINDEIKIYKAGDVIPYVDGVDLSKRLANSVPYEPIINCPSCQSVLVRENDEVDQRC
CCCEECCCEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHCCCCCHHHHHH
LNIYGCKKINIEKIVYFVSRNCMNIEGMSDAIINKFYDANLIKNIADLYYLQKHKEFILT
HHHHCCCEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
SDFKIKEKSFSNLINSINNSKKCSLEFLLTAFGIRHVGPNLAKKIAKQFKTMTALMHANF
CCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCH
DELTNVDACGEKAALSLINWFNDEHNVSLVNQLQQVGVNMEYIDDFIYDDNINIIDEYKN
HHHCCCHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCEEECCCC
KTFVITGSFSISRDEIKTILEKYYHAKVKNSVSKKTDYVLVGTEAGTKLEKAKLLGVKII
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHH
ENEFKKDNNF
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA