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The map label for this gene is gadC [H]

Identifier: 170762191

GI number: 170762191

Start: 267924

End: 270365

Strand: Direct

Name: gadC [H]

Synonym: UPA3_0230

Alternate gene names: 170762191

Gene position: 267924-270365 (Clockwise)

Preceding gene: 170762132

Following gene: 170762166

Centisome position: 35.64

GC content: 30.79

Gene sequence:

>2442_bases
ATGAATTTATGAGCAAATTTTTGATTGGTCGTAAGCGATTTATCAAAAATAAAATTAGTTTCAAATATTTTTAGAATAAG
AGGTGATAAAAAATTGATAAATAATAAAATAAAAGAAGCAAAAATCAAAAAGAAACTAGAAAAAAACAAAAAAGTTTCTT
TAATGGGTTTTGTTTTACTAACAGCTTCAGCAATTATTGCTTTTTATACATTCCCATCACTTTCAACAGCTGGTTGAATT
GCAATTATCTTTGCTTTTATTACAGCTATGTGTTGATTCATTCCAATTGGGATTGCAGCCGCTGAAATGGCAACAATTAA
AGGTTGGGCCGAGGGGGGAATATTTACCTGGGTACGAAATTTATTAGGTCCACGAAATGGGTTTATTGTAACCTGATTAC
AATTCCAAGTCACATTTGGATTTGTGGCTATGATCTTATTCGTCCTTTCATCCTTTTCATTTGCTTTCGCAGGTGCTGAT
GGATATAATTATTTTAATACACTTAAGTTGGGAACAACTTTAAATCAAGCTACATCAGTCAACTTTTCAAAAAACTTTAG
CAATGGGGCATTATATGGAATTGGTATTGTTGTTGTCGCAGGTCTAATCTTTATGAGTTTATTAGGCCAAAAACGAACTC
ACCAATTTGGCCAAGTTGCTTTAATCATTGGTATTTTACTACCATTCTTTATTATTTTAGGATTCCAAATTTATACAGTG
GCAACAATGCCAGATCCATTATACTTTATTGGAAAACACTTCCAAAAAGAACCACATTTAGACCCAAGTGTAACTGGTAC
TTCTTATGAATTAAATAAAACTTTCATGTCATCAGTTGTTATGGCATCTTTCATGGCATTTAGTTTTAGCTTACATGGTG
TAGAAGTTTCAGCTATTGCTGCTAATCGAATGGATAATCCTTCTAAGAAATATCCACAAGCCATGATGATTGTTGTAATT
TCTGCTTTAGTTTGCATTATTATTGGATCAATTATGATTTCAATGACTGTACCAACTTCAACATTATCATTCAATGGTGG
ATTAGTACAGACAATTATGTTCAACTTATCTGTTGGTGGAATTGATGTTAAACTACCAGATGGCACAGTTGCTCATTATA
AAACACTAGCACATGCATACTCAGTAATTGAAACTCAATATGGCACAGCTGCCGCTAACCAAGTTATGTATCAATTTGTG
TATGGTCATCCAATTAATACAGGTAGTGACTTAGCACGTTCTGATTTATTAACACTAACATCAGAAGTTAACGATCCTTT
AAAAAACCCATCTTTATTTATTTCTGTTATGAATATAGAAACAAATAAAATGGCTATGCAAGGATTACAAGCTATTACTT
TCTTCATTGGAATAGGTGTATTTGTTGAAGTTTCTGTTTGAATGTCAAACCTTTCAACTGGATTAAATTATGCAATGCGT
AAAGCCCACTTCCCAGCATGGGTTACATATCGTTTAAAGAATGGGTCAGCGTTAACAGTAGCAATTTTATGAATTGTATT
ACTAATGTGCATTTTTGCAATTTACATGTTTGGTTATAATTCATTAATGCAATACAAAGGTCAAACAATTAGTGATATTT
TAGCTAATGAAATTCGTATTAACCAAGATCAAAAAGAACCAATGGCTTTATTGTCTAATCAAAATTTAGATGAAATTAAA
CTAATGGCTCGTTCAATTTACGAACATAGTCATGCTCCTGGAGCACAAGCTAATGTTTTACATTTTGATTTTAATAAACA
TGCTAACAGTGAAGTAAAAGCAATTTATGATAAAATCAAAGATTATATTGGCATTAATGCCGTAGCATCGGGAACACCAG
CATCAATTACTAATATTTCATTCATTTCAAACGTTGTTTCACAAATCTCAATGTATTATATTGGATATAACATTTTCTTA
ATTGGATATCTAAGATATGTCTTTAAAGCTAATTGATTACGTCGTGAATTTAGAGTTAAATGATCATGATTACAAATTAG
TTTAGCTTTATTAGCAATGGGCGTTAATACATTTGCAGTTGTTGCAACTTACTTACCAGCTGCTCCAGAACTATATCCTG
GACAAGAAGCTTATTATGTATTTATTTCAATTGCATTACCAATGTTTATATGCACATTATTACTTGGATTATTTATTTAT
CTAATTAATTATGCTTGAAATAAAAAACGAGGTATTAATTTATCAGAACATTCAAACTTAACAAACGTTGATCAAAATGT
TTTAGATGTAATTAATAATTGACATTTATACAAAGATCAAAAAGAATTTGCTAAATTTGAACGTCATGTGCAATTATTAC
AACAAAAACAAGTAATTGTAAGAACAACAAAAGATAAGGCACAAAGAGAATTAGTTGCGAATGAAATGCAATATTTAGCA
GCTGAAATCGCAACAAGATTTGCACCAGAAGAAGAACATTAA

Upstream 100 bases:

>100_bases
AATAAAAATGTTAAAAATCGACATTTTTATGCTAAAAAGCATAGGAAATACTAGCATTTATTATATAATCTAAATTAGAA
ATTAGGTTTTATATATAGTA

Downstream 100 bases:

>100_bases
AAAACAAAAATGAGTCTTAATACTTTTTTAAAAAGTGTTTTGACTTATTTTTTTATTTTACACTTGACATATTATATATA
TATATATAATCTAAATGTAT

Product: amino acid antiporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: Extreme acid sensitivity protein [H]

Number of amino acids: Translated: 813; Mature: 813

Protein sequence:

>813_residues
MNLWANFWLVVSDLSKIKLVSNIFRIRGDKKLINNKIKEAKIKKKLEKNKKVSLMGFVLLTASAIIAFYTFPSLSTAGWI
AIIFAFITAMCWFIPIGIAAAEMATIKGWAEGGIFTWVRNLLGPRNGFIVTWLQFQVTFGFVAMILFVLSSFSFAFAGAD
GYNYFNTLKLGTTLNQATSVNFSKNFSNGALYGIGIVVVAGLIFMSLLGQKRTHQFGQVALIIGILLPFFIILGFQIYTV
ATMPDPLYFIGKHFQKEPHLDPSVTGTSYELNKTFMSSVVMASFMAFSFSLHGVEVSAIAANRMDNPSKKYPQAMMIVVI
SALVCIIIGSIMISMTVPTSTLSFNGGLVQTIMFNLSVGGIDVKLPDGTVAHYKTLAHAYSVIETQYGTAAANQVMYQFV
YGHPINTGSDLARSDLLTLTSEVNDPLKNPSLFISVMNIETNKMAMQGLQAITFFIGIGVFVEVSVWMSNLSTGLNYAMR
KAHFPAWVTYRLKNGSALTVAILWIVLLMCIFAIYMFGYNSLMQYKGQTISDILANEIRINQDQKEPMALLSNQNLDEIK
LMARSIYEHSHAPGAQANVLHFDFNKHANSEVKAIYDKIKDYIGINAVASGTPASITNISFISNVVSQISMYYIGYNIFL
IGYLRYVFKANWLRREFRVKWSWLQISLALLAMGVNTFAVVATYLPAAPELYPGQEAYYVFISIALPMFICTLLLGLFIY
LINYAWNKKRGINLSEHSNLTNVDQNVLDVINNWHLYKDQKEFAKFERHVQLLQQKQVIVRTTKDKAQRELVANEMQYLA
AEIATRFAPEEEH

Sequences:

>Translated_813_residues
MNL*ANF*LVVSDLSKIKLVSNIFRIRGDKKLINNKIKEAKIKKKLEKNKKVSLMGFVLLTASAIIAFYTFPSLSTAG*I
AIIFAFITAMC*FIPIGIAAAEMATIKGWAEGGIFTWVRNLLGPRNGFIVT*LQFQVTFGFVAMILFVLSSFSFAFAGAD
GYNYFNTLKLGTTLNQATSVNFSKNFSNGALYGIGIVVVAGLIFMSLLGQKRTHQFGQVALIIGILLPFFIILGFQIYTV
ATMPDPLYFIGKHFQKEPHLDPSVTGTSYELNKTFMSSVVMASFMAFSFSLHGVEVSAIAANRMDNPSKKYPQAMMIVVI
SALVCIIIGSIMISMTVPTSTLSFNGGLVQTIMFNLSVGGIDVKLPDGTVAHYKTLAHAYSVIETQYGTAAANQVMYQFV
YGHPINTGSDLARSDLLTLTSEVNDPLKNPSLFISVMNIETNKMAMQGLQAITFFIGIGVFVEVSV*MSNLSTGLNYAMR
KAHFPAWVTYRLKNGSALTVAIL*IVLLMCIFAIYMFGYNSLMQYKGQTISDILANEIRINQDQKEPMALLSNQNLDEIK
LMARSIYEHSHAPGAQANVLHFDFNKHANSEVKAIYDKIKDYIGINAVASGTPASITNISFISNVVSQISMYYIGYNIFL
IGYLRYVFKAN*LRREFRVK*S*LQISLALLAMGVNTFAVVATYLPAAPELYPGQEAYYVFISIALPMFICTLLLGLFIY
LINYA*NKKRGINLSEHSNLTNVDQNVLDVINN*HLYKDQKEFAKFERHVQLLQQKQVIVRTTKDKAQRELVANEMQYLA
AEIATRFAPEEEH
>Mature_813_residues
MNL*ANF*LVVSDLSKIKLVSNIFRIRGDKKLINNKIKEAKIKKKLEKNKKVSLMGFVLLTASAIIAFYTFPSLSTAG*I
AIIFAFITAMC*FIPIGIAAAEMATIKGWAEGGIFTWVRNLLGPRNGFIVT*LQFQVTFGFVAMILFVLSSFSFAFAGAD
GYNYFNTLKLGTTLNQATSVNFSKNFSNGALYGIGIVVVAGLIFMSLLGQKRTHQFGQVALIIGILLPFFIILGFQIYTV
ATMPDPLYFIGKHFQKEPHLDPSVTGTSYELNKTFMSSVVMASFMAFSFSLHGVEVSAIAANRMDNPSKKYPQAMMIVVI
SALVCIIIGSIMISMTVPTSTLSFNGGLVQTIMFNLSVGGIDVKLPDGTVAHYKTLAHAYSVIETQYGTAAANQVMYQFV
YGHPINTGSDLARSDLLTLTSEVNDPLKNPSLFISVMNIETNKMAMQGLQAITFFIGIGVFVEVSV*MSNLSTGLNYAMR
KAHFPAWVTYRLKNGSALTVAIL*IVLLMCIFAIYMFGYNSLMQYKGQTISDILANEIRINQDQKEPMALLSNQNLDEIK
LMARSIYEHSHAPGAQANVLHFDFNKHANSEVKAIYDKIKDYIGINAVASGTPASITNISFISNVVSQISMYYIGYNIFL
IGYLRYVFKAN*LRREFRVK*S*LQISLALLAMGVNTFAVVATYLPAAPELYPGQEAYYVFISIALPMFICTLLLGLFIY
LINYA*NKKRGINLSEHSNLTNVDQNVLDVINN*HLYKDQKEFAKFERHVQLLQQKQVIVRTTKDKAQRELVANEMQYLA
AEIATRFAPEEEH

Specific function: Involved in glutamate-dependent acid resistance. Imports glutamate inside the cell while simultaneously exporting to the periplasm the GABA produced by gadA and gadB. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Glutamate:GABA antiporter (GGA) (TC 2.A.3.7) family [H]

Homologues:

Organism=Escherichia coli, GI1787768, Length=307, Percent_Identity=32.2475570032573, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004759 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 88629; Mature: 88629

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLANFLVVSDLSKIKLVSNIFRIRGDKKLINNKIKEAKIKKKLEKNKKVSLMGFVLLTA
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SAIIAFYTFPSLSTAGIAIIFAFITAMCFIPIGIAAAEMATIKGWAEGGIFTWVRNLLGP
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
RNGFIVTLQFQVTFGFVAMILFVLSSFSFAFAGADGYNYFNTLKLGTTLNQATSVNFSKN
CCCEEEEEEEEHHHHHHHHHHHHHHCCCCEEECCCCCCHHHEEEECCCCCHHHCCCCCCC
FSNGALYGIGIVVVAGLIFMSLLGQKRTHQFGQVALIIGILLPFFIILGFQIYTVATMPD
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
PLYFIGKHFQKEPHLDPSVTGTSYELNKTFMSSVVMASFMAFSFSLHGVEVSAIAANRMD
CHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHEECCEEEEEEECCCCC
NPSKKYPQAMMIVVISALVCIIIGSIMISMTVPTSTLSFNGGLVQTIMFNLSVGGIDVKL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCHHHHHHHHEEECCEEEEC
PDGTVAHYKTLAHAYSVIETQYGTAAANQVMYQFVYGHPINTGSDLARSDLLTLTSEVND
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
PLKNPSLFISVMNIETNKMAMQGLQAITFFIGIGVFVEVSVMSNLSTGLNYAMRKAHFPA
CCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
WVTYRLKNGSALTVAILIVLLMCIFAIYMFGYNSLMQYKGQTISDILANEIRINQDQKEP
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCH
MALLSNQNLDEIKLMARSIYEHSHAPGAQANVLHFDFNKHANSEVKAIYDKIKDYIGINA
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCC
VASGTPASITNISFISNVVSQISMYYIGYNIFLIGYLRYVFKANLRREFRVKSLQISLAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAMGVNTFAVVATYLPAAPELYPGQEAYYVFISIALPMFICTLLLGLFIYLINYANKKRG
HHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
INLSEHSNLTNVDQNVLDVINNHLYKDQKEFAKFERHVQLLQQKQVIVRTTKDKAQRELV
CCCHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
ANEMQYLAAEIATRFAPEEEH
HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNLANFLVVSDLSKIKLVSNIFRIRGDKKLINNKIKEAKIKKKLEKNKKVSLMGFVLLTA
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SAIIAFYTFPSLSTAGIAIIFAFITAMCFIPIGIAAAEMATIKGWAEGGIFTWVRNLLGP
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
RNGFIVTLQFQVTFGFVAMILFVLSSFSFAFAGADGYNYFNTLKLGTTLNQATSVNFSKN
CCCEEEEEEEEHHHHHHHHHHHHHHCCCCEEECCCCCCHHHEEEECCCCCHHHCCCCCCC
FSNGALYGIGIVVVAGLIFMSLLGQKRTHQFGQVALIIGILLPFFIILGFQIYTVATMPD
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
PLYFIGKHFQKEPHLDPSVTGTSYELNKTFMSSVVMASFMAFSFSLHGVEVSAIAANRMD
CHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHEECCEEEEEEECCCCC
NPSKKYPQAMMIVVISALVCIIIGSIMISMTVPTSTLSFNGGLVQTIMFNLSVGGIDVKL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCHHHHHHHHEEECCEEEEC
PDGTVAHYKTLAHAYSVIETQYGTAAANQVMYQFVYGHPINTGSDLARSDLLTLTSEVND
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
PLKNPSLFISVMNIETNKMAMQGLQAITFFIGIGVFVEVSVMSNLSTGLNYAMRKAHFPA
CCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
WVTYRLKNGSALTVAILIVLLMCIFAIYMFGYNSLMQYKGQTISDILANEIRINQDQKEP
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCH
MALLSNQNLDEIKLMARSIYEHSHAPGAQANVLHFDFNKHANSEVKAIYDKIKDYIGINA
HHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCC
VASGTPASITNISFISNVVSQISMYYIGYNIFLIGYLRYVFKANLRREFRVKSLQISLAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAMGVNTFAVVATYLPAAPELYPGQEAYYVFISIALPMFICTLLLGLFIYLINYANKKRG
HHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
INLSEHSNLTNVDQNVLDVINNHLYKDQKEFAKFERHVQLLQQKQVIVRTTKDKAQRELV
CCCHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
ANEMQYLAAEIATRFAPEEEH
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]