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The map label for this gene is pheT [C]

Identifier: 170761884

GI number: 170761884

Start: 235604

End: 236197

Strand: Direct

Name: pheT [C]

Synonym: UPA3_0198

Alternate gene names: 170761884

Gene position: 235604-236197 (Clockwise)

Preceding gene: 170762088

Following gene: 170762174

Centisome position: 31.34

GC content: 23.4

Gene sequence:

>594_bases
ATGTATATTTATTATAATAAAACAAGTCTAAACGATACGTTAATTTTTTTAAAAAACAATCAGCAATATGATCATCAAGT
GTTTTTAAACGATGACTTATTGCTTTTTTATCATGGTGATGAACTAATTGGTTTAAATTTATTTAATGCAAGTGAATATT
TAAAAAATTTAAATGAAGGATATTTATATCCTACGACTCAATTAATTCAAAAATTAAGTGATTTAATAAAAATAAAATTA
TCATTAGATAATGCTTTTAAAGGTTTTGTGGTAGGTAAAATTTTAGAAGTTAATTTAATTCCCAATACTCATTTACATAT
TTGCTTAGTAGATATAGGTGATCAACAAATACAAATAATATGTGGAGCACAAAATGTCCGTATTGGCTTAAAAACGGTTG
TAGCGACACCTAATTTATTAATGCCAAATGGAAATGAAATTAAAAAATCCAAATTAATGAACTATGATTCATTTGGAATG
TTGTGTTCACAAAAAGAATTAAATATTAATAATTTTAATTCACAAGGAATTGTCGAACTTAATGATGAATATCAGGTTGG
TCAATTGTTTACAAAAGTTTATAGTAATTTATAA

Upstream 100 bases:

>100_bases
AAACAACATTATTAACTATGATTAAAAGTTAGATAAAGTTAATTTAATATTATAATTTATAAATAAAGAGAGTAATTTCT
CTTTAAAAATTGGAGTTGAA

Downstream 100 bases:

>100_bases
TTTAAAGTAGGTAAAATTAATGTCAAAAAATGATGATATATTATTATTTAGTCTTTCAAATTCACATCAATTAGCTAATA
AAATTGCTAATTTACTAAAA

Product: tRNA-binding domain-containing protein

Products: AMP; diphosphate; L-phenylalanyl-tRNAPhe [C]

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MYIYYNKTSLNDTLIFLKNNQQYDHQVFLNDDLLLFYHGDELIGLNLFNASEYLKNLNEGYLYPTTQLIQKLSDLIKIKL
SLDNAFKGFVVGKILEVNLIPNTHLHICLVDIGDQQIQIICGAQNVRIGLKTVVATPNLLMPNGNEIKKSKLMNYDSFGM
LCSQKELNINNFNSQGIVELNDEYQVGQLFTKVYSNL

Sequences:

>Translated_197_residues
MYIYYNKTSLNDTLIFLKNNQQYDHQVFLNDDLLLFYHGDELIGLNLFNASEYLKNLNEGYLYPTTQLIQKLSDLIKIKL
SLDNAFKGFVVGKILEVNLIPNTHLHICLVDIGDQQIQIICGAQNVRIGLKTVVATPNLLMPNGNEIKKSKLMNYDSFGM
LCSQKELNINNFNSQGIVELNDEYQVGQLFTKVYSNL
>Mature_197_residues
MYIYYNKTSLNDTLIFLKNNQQYDHQVFLNDDLLLFYHGDELIGLNLFNASEYLKNLNEGYLYPTTQLIQKLSDLIKIKL
SLDNAFKGFVVGKILEVNLIPNTHLHICLVDIGDQQIQIICGAQNVRIGLKTVVATPNLLMPNGNEIKKSKLMNYDSFGM
LCSQKELNINNFNSQGIVELNDEYQVGQLFTKVYSNL

Specific function: Unknown

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=89, Percent_Identity=41.5730337078652, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR002547 [H]

Pfam domain/function: PF01588 tRNA_bind [H]

EC number: 6.1.1.20 [C]

Molecular weight: Translated: 22532; Mature: 22532

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIYYNKTSLNDTLIFLKNNQQYDHQVFLNDDLLLFYHGDELIGLNLFNASEYLKNLNEG
CEEEEECCCCCEEEEEEECCCCCCEEEEECCCEEEEEECCEEEEEEEECHHHHHHCCCCC
YLYPTTQLIQKLSDLIKIKLSLDNAFKGFVVGKILEVNLIPNTHLHICLVDIGDQQIQII
EECCHHHHHHHHHHHEEEEEEECCCCCCEEEEEEEEEEECCCCCEEEEEEEECCCEEEEE
CGAQNVRIGLKTVVATPNLLMPNGNEIKKSKLMNYDSFGMLCSQKELNINNFNSQGIVEL
ECCCCEEEEEEEEEECCCEECCCCCCHHHHHHCCCCCCCEEECCCCCCCCCCCCCCEEEE
NDEYQVGQLFTKVYSNL
CCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MYIYYNKTSLNDTLIFLKNNQQYDHQVFLNDDLLLFYHGDELIGLNLFNASEYLKNLNEG
CEEEEECCCCCEEEEEEECCCCCCEEEEECCCEEEEEECCEEEEEEEECHHHHHHCCCCC
YLYPTTQLIQKLSDLIKIKLSLDNAFKGFVVGKILEVNLIPNTHLHICLVDIGDQQIQII
EECCHHHHHHHHHHHEEEEEEECCCCCCEEEEEEEEEEECCCCCEEEEEEEECCCEEEEE
CGAQNVRIGLKTVVATPNLLMPNGNEIKKSKLMNYDSFGMLCSQKELNINNFNSQGIVEL
ECCCCEEEEEEEEEECCCEECCCCCCHHHHHHCCCCCCCEEECCCCCCCCCCCCCCEEEE
NDEYQVGQLFTKVYSNL
CCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.125 {2'-deoxyadenosine} 0.009 {ATP}} 0.2 {2-chloroadenosine} 0.11 {2-chloroadenosine} [C]

Substrates: ATP; @PHEN01.txt*phenylalanine!; tRNAPhe [C]

Specific reaction: ATP + @PHEN01.txt*phenylalanine! + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 3'-Deoxyadenosine 5'-triphosphate; 3'-Methyl adenosine 5'-triphosphate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7569993 [H]