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The map label for this gene is ychF

Identifier: 170757373

GI number: 170757373

Start: 1615587

End: 1616684

Strand: Reverse

Name: ychF

Synonym: CLD_3098

Alternate gene names: 170757373

Gene position: 1616684-1615587 (Counterclockwise)

Preceding gene: 170755109

Following gene: 170755086

Centisome position: 40.84

GC content: 31.24

Gene sequence:

>1098_bases
ATGAAATTAGGAATAGTTGGTCTACCTAACGTAGGTAAAAGTACATTATTTAATGCAATAACAAAAGCAGGAGCTGAATC
TGCTAACTATCCATTCTGTACCATAGAACCAAACGTAGGGGTAGTTAGTGTACCAGATAAAAGGTTAGATGTTTTAGAAA
AAATGTATAACTCTAAAAAGAAGGTTCCTACTGCTATTGAGTTTTATGATATTGCTGGATTAGTTAAAGGAGCAAGTAAA
GGAGAAGGATTAGGAAATAAATTTTTATCTCATATAAGGGAAGTTGAAGCTATTGTTCATGTAGTAAGATGTTTTGAAGA
TTCAAATATAGTACACGTAGATGGCTCTGTAGATCCTATTCGTGATATAGAAACTATTAGTTTAGAGCTTATATTTTCTG
ATATAGAATTAATGCAAAGAAGAATTGAAAAAAATAGTAAACTAGCTAAAAGTGGGAATAAAGAAGCCAAAGCAGAAGAA
GCTCTCATGAAGAGAATAGTTGCTCATTTAGAAGAAGGTAAGCCTGTTAGAACGTTGGAACTTGAAGAAGATGAAGAGAA
ATTAGTTAAAAGTTATTTCCTTTTAACCTCAAAACCTGTTTTATATGCAGCTAATGTATCTGAAGATGATTTAATGTCTG
GAAATCCTGAAAATGAGTTTGTTAAAAAAGTTAAAGAATTTGCAAAAGCTGAAAATTCTGAAGTGATAACTTTATGTGCA
AGACTTGAAGAAGAATTGTCAACTTTAGAAGATGATGAAAAAGCTGAAATGCTTTCAGAATATGGATTAAAAGAATCTGG
CCTTGATAAACTAGTTCAATCTAGTTATAAATTATTAGGCCTTATAAGCTTTTTAACTGCAGGTCAAGTTGAAGTTAGAG
CTTGGACTATAGTTAAAGGTACAAAAGCACCTAAAGCTGCTGGTAAAATTCATACTGATATTGAAAAAGGTTTTATAAGA
GCAGAAGTTATATCCTACGATAAACTTATAGAATGTGGTTCTGAAGCCCATGCTAAAGAAAAAGGATTCTTTAGACTTGA
AGGAAAAGAATATGTAATGCAAGATGGAGATATTGTAAACTTTAGATTTAACGTTTAA

Upstream 100 bases:

>100_bases
TAACCTAAATTATTTTTATTCAAATATAAATTGTTTTATCAATTATTTAGATTTTTTTAATTACATAAAGTCAATATACA
AAGAAAGGACTGTACATAAT

Downstream 100 bases:

>100_bases
TCTATCTCATTAATAAAAAATCACTATTGGTTAGCAATGGTGATTTTTTATTAATGTATAGTACATACTTTTACCATTAT
AGTATAATATAATATATATT

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKLGIVGLPNVGKSTLFNAITKAGAESANYPFCTIEPNVGVVSVPDKRLDVLEKMYNSKKKVPTAIEFYDIAGLVKGASK
GEGLGNKFLSHIREVEAIVHVVRCFEDSNIVHVDGSVDPIRDIETISLELIFSDIELMQRRIEKNSKLAKSGNKEAKAEE
ALMKRIVAHLEEGKPVRTLELEEDEEKLVKSYFLLTSKPVLYAANVSEDDLMSGNPENEFVKKVKEFAKAENSEVITLCA
RLEEELSTLEDDEKAEMLSEYGLKESGLDKLVQSSYKLLGLISFLTAGQVEVRAWTIVKGTKAPKAAGKIHTDIEKGFIR
AEVISYDKLIECGSEAHAKEKGFFRLEGKEYVMQDGDIVNFRFNV

Sequences:

>Translated_365_residues
MKLGIVGLPNVGKSTLFNAITKAGAESANYPFCTIEPNVGVVSVPDKRLDVLEKMYNSKKKVPTAIEFYDIAGLVKGASK
GEGLGNKFLSHIREVEAIVHVVRCFEDSNIVHVDGSVDPIRDIETISLELIFSDIELMQRRIEKNSKLAKSGNKEAKAEE
ALMKRIVAHLEEGKPVRTLELEEDEEKLVKSYFLLTSKPVLYAANVSEDDLMSGNPENEFVKKVKEFAKAENSEVITLCA
RLEEELSTLEDDEKAEMLSEYGLKESGLDKLVQSSYKLLGLISFLTAGQVEVRAWTIVKGTKAPKAAGKIHTDIEKGFIR
AEVISYDKLIECGSEAHAKEKGFFRLEGKEYVMQDGDIVNFRFNV
>Mature_365_residues
MKLGIVGLPNVGKSTLFNAITKAGAESANYPFCTIEPNVGVVSVPDKRLDVLEKMYNSKKKVPTAIEFYDIAGLVKGASK
GEGLGNKFLSHIREVEAIVHVVRCFEDSNIVHVDGSVDPIRDIETISLELIFSDIELMQRRIEKNSKLAKSGNKEAKAEE
ALMKRIVAHLEEGKPVRTLELEEDEEKLVKSYFLLTSKPVLYAANVSEDDLMSGNPENEFVKKVKEFAKAENSEVITLCA
RLEEELSTLEDDEKAEMLSEYGLKESGLDKLVQSSYKLLGLISFLTAGQVEVRAWTIVKGTKAPKAAGKIHTDIEKGFIR
AEVISYDKLIECGSEAHAKEKGFFRLEGKEYVMQDGDIVNFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=370, Percent_Identity=45.945945945946, Blast_Score=315, Evalue=3e-86,
Organism=Homo sapiens, GI58761502, Length=229, Percent_Identity=40.6113537117904, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI24308117, Length=111, Percent_Identity=35.1351351351351, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI111955139, Length=100, Percent_Identity=34, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1787454, Length=365, Percent_Identity=53.1506849315069, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1789574, Length=152, Percent_Identity=31.5789473684211, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17509631, Length=367, Percent_Identity=45.2316076294278, Blast_Score=321, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI17508313, Length=99, Percent_Identity=36.3636363636364, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319499, Length=371, Percent_Identity=43.6657681940701, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6321773, Length=389, Percent_Identity=39.8457583547558, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6321649, Length=338, Percent_Identity=27.5147928994083, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24640873, Length=372, Percent_Identity=45.1612903225806, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24640877, Length=372, Percent_Identity=45.1612903225806, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24640875, Length=372, Percent_Identity=45.1612903225806, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24640879, Length=331, Percent_Identity=43.8066465256798, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24585318, Length=103, Percent_Identity=36.8932038834951, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40569; Mature: 40569

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGIVGLPNVGKSTLFNAITKAGAESANYPFCTIEPNVGVVSVPDKRLDVLEKMYNSKK
CCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHCCCC
KVPTAIEFYDIAGLVKGASKGEGLGNKFLSHIREVEAIVHVVRCFEDSNIVHVDGSVDPI
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
RDIETISLELIFSDIELMQRRIEKNSKLAKSGNKEAKAEEALMKRIVAHLEEGKPVRTLE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEE
LEEDEEKLVKSYFLLTSKPVLYAANVSEDDLMSGNPENEFVKKVKEFAKAENSEVITLCA
CCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
RLEEELSTLEDDEKAEMLSEYGLKESGLDKLVQSSYKLLGLISFLTAGQVEVRAWTIVKG
HHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEC
TKAPKAAGKIHTDIEKGFIRAEVISYDKLIECGSEAHAKEKGFFRLEGKEYVMQDGDIVN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEECCCCEEECCCCEEE
FRFNV
EEECC
>Mature Secondary Structure
MKLGIVGLPNVGKSTLFNAITKAGAESANYPFCTIEPNVGVVSVPDKRLDVLEKMYNSKK
CCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHCCCC
KVPTAIEFYDIAGLVKGASKGEGLGNKFLSHIREVEAIVHVVRCFEDSNIVHVDGSVDPI
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
RDIETISLELIFSDIELMQRRIEKNSKLAKSGNKEAKAEEALMKRIVAHLEEGKPVRTLE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEE
LEEDEEKLVKSYFLLTSKPVLYAANVSEDDLMSGNPENEFVKKVKEFAKAENSEVITLCA
CCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
RLEEELSTLEDDEKAEMLSEYGLKESGLDKLVQSSYKLLGLISFLTAGQVEVRAWTIVKG
HHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEC
TKAPKAAGKIHTDIEKGFIRAEVISYDKLIECGSEAHAKEKGFFRLEGKEYVMQDGDIVN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEECCCCEEECCCCEEE
FRFNV
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]