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The map label for this gene is prpB [C]

Identifier: 170756847

GI number: 170756847

Start: 1006754

End: 1007653

Strand: Reverse

Name: prpB [C]

Synonym: CLD_3671

Alternate gene names: 170756847

Gene position: 1007653-1006754 (Counterclockwise)

Preceding gene: 170755016

Following gene: 170755709

Centisome position: 25.46

GC content: 30.78

Gene sequence:

>900_bases
ATGAATCATAAAATTATAAATCCTATAAATAATAAAAATACTTATGAAATGGGGACAAATATGGATATTACAAAGCAATT
ATACTTAGCAAAACAATTTCAAGAAATTCATCATCGTAATAAAATGTTTATCCTTCCTAATGCGTGGGATATTGGTAGTG
CAGTTATATATGAAAAATTTAATTTTCCAGCAGTTGGAACAACAAGTGCTGGGCTTGCTTATAGTTTAGGCTATTCTGAT
GGAGAAAAAATTGATTTTGAACATATTGTTCAAATAGTAAATCAAATAACTCAAAGGATATCTATTCCTGTGAGTGTAGA
CATTGAATTAGGTTATGCTGACACAATTGATGGAATAGTGGAAAATGTAACTAAGATTATTGAAGCTGGTGCAGTTGGAA
TCAACATTGAAGATGGCTGTTCCAAACCAACTCCTTACCTAGAAAATCTAGATATTCAAATAAAAAAAATCCAGGCTATA
TCTAAGCTTAAAGAAAAGTTGGGAATTCCTTTTGTTCTTAATGCCAGAACTTGCGTGTTTTTTATTAAAGCAGGAAAAGA
AGAAGAAAGGTTATCTATTGCTATTGAAAGAGCCAATGCTTTTCATAAATCAGGTGCTGATTGTATTTTTATACCTGGTG
CAATTGAAGAATGTAATATTTCAAAATTAATAGAAAATATTCCTGCCCCTATTAATATTCTTGCCACACCTATGACAAAT
AATTTAGAAAATTTAGAACGATTAGGAGTTAGACGATTAAGCCTTGGTTCTGCTCCGGTTAGAGCAGCTTATTCTAACCT
AATAGAAACAGTAAAGCAGATACAGGATGAGAACAAAATTTCAAAATTACTAGATCACGATTTTTCTTACGAAATAGCTA
ATAAACTATTTGCACGTTAA

Upstream 100 bases:

>100_bases
TTCCAGTCATATATATCCTCCTAATAATTTCATATATCGATGAGCATTGATGTATCTATAGTATATAATAACTTATACAA
ATTGTACAAGGGGTGAGTTT

Downstream 100 bases:

>100_bases
CTATATAAATATAACTCAAGTGTATAAATCATTAGTAAAATGCTAAAATAGCATATAATAATGTCCCAATTAAATTATAT
AGAATATTAATACGAGTTTA

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNHKIINPINNKNTYEMGTNMDITKQLYLAKQFQEIHHRNKMFILPNAWDIGSAVIYEKFNFPAVGTTSAGLAYSLGYSD
GEKIDFEHIVQIVNQITQRISIPVSVDIELGYADTIDGIVENVTKIIEAGAVGINIEDGCSKPTPYLENLDIQIKKIQAI
SKLKEKLGIPFVLNARTCVFFIKAGKEEERLSIAIERANAFHKSGADCIFIPGAIEECNISKLIENIPAPINILATPMTN
NLENLERLGVRRLSLGSAPVRAAYSNLIETVKQIQDENKISKLLDHDFSYEIANKLFAR

Sequences:

>Translated_299_residues
MNHKIINPINNKNTYEMGTNMDITKQLYLAKQFQEIHHRNKMFILPNAWDIGSAVIYEKFNFPAVGTTSAGLAYSLGYSD
GEKIDFEHIVQIVNQITQRISIPVSVDIELGYADTIDGIVENVTKIIEAGAVGINIEDGCSKPTPYLENLDIQIKKIQAI
SKLKEKLGIPFVLNARTCVFFIKAGKEEERLSIAIERANAFHKSGADCIFIPGAIEECNISKLIENIPAPINILATPMTN
NLENLERLGVRRLSLGSAPVRAAYSNLIETVKQIQDENKISKLLDHDFSYEIANKLFAR
>Mature_299_residues
MNHKIINPINNKNTYEMGTNMDITKQLYLAKQFQEIHHRNKMFILPNAWDIGSAVIYEKFNFPAVGTTSAGLAYSLGYSD
GEKIDFEHIVQIVNQITQRISIPVSVDIELGYADTIDGIVENVTKIIEAGAVGINIEDGCSKPTPYLENLDIQIKKIQAI
SKLKEKLGIPFVLNARTCVFFIKAGKEEERLSIAIERANAFHKSGADCIFIPGAIEECNISKLIENIPAPINILATPMTN
NLENLERLGVRRLSLGSAPVRAAYSNLIETVKQIQDENKISKLLDHDFSYEIANKLFAR

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=198, Percent_Identity=27.2727272727273, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 33377; Mature: 33377

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHKIINPINNKNTYEMGTNMDITKQLYLAKQFQEIHHRNKMFILPNAWDIGSAVIYEKF
CCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEECC
NFPAVGTTSAGLAYSLGYSDGEKIDFEHIVQIVNQITQRISIPVSVDIELGYADTIDGIV
CCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHH
ENVTKIIEAGAVGINIEDGCSKPTPYLENLDIQIKKIQAISKLKEKLGIPFVLNARTCVF
HHHHHHHHCCCEEEEECCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCEEECCCEEEE
FIKAGKEEERLSIAIERANAFHKSGADCIFIPGAIEECNISKLIENIPAPINILATPMTN
EEECCCCCHHHHEEHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCC
NLENLERLGVRRLSLGSAPVRAAYSNLIETVKQIQDENKISKLLDHDFSYEIANKLFAR
CHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNHKIINPINNKNTYEMGTNMDITKQLYLAKQFQEIHHRNKMFILPNAWDIGSAVIYEKF
CCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEECC
NFPAVGTTSAGLAYSLGYSDGEKIDFEHIVQIVNQITQRISIPVSVDIELGYADTIDGIV
CCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHH
ENVTKIIEAGAVGINIEDGCSKPTPYLENLDIQIKKIQAISKLKEKLGIPFVLNARTCVF
HHHHHHHHCCCEEEEECCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCEEECCCEEEE
FIKAGKEEERLSIAIERANAFHKSGADCIFIPGAIEECNISKLIENIPAPINILATPMTN
EEECCCCCHHHHEEHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCC
NLENLERLGVRRLSLGSAPVRAAYSNLIETVKQIQDENKISKLLDHDFSYEIANKLFAR
CHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]