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The map label for this gene is vanS [H]

Identifier: 170755566

GI number: 170755566

Start: 1786185

End: 1788287

Strand: Reverse

Name: vanS [H]

Synonym: CLD_2943

Alternate gene names: 170755566

Gene position: 1788287-1786185 (Counterclockwise)

Preceding gene: 170757144

Following gene: 170757085

Centisome position: 45.18

GC content: 23.68

Gene sequence:

>2103_bases
ATGCTATCTCTTTCAATTTTAACTGGATATGATTTAATAAAAAACAGAAAATATCTTGTTAAATCTCCTTATTTTAATAG
TTATGAGTTTGACATGGAAATATATTCTTATTGTTCAAACTTGCATAACTTTCATATAGCCTATAAAAATTTTGATTATA
AAGTAGCAGAAAATAAGGTTACTAAAGAACAATTAGCAGACTTAAAACTATTTTATGAAGATATGATAAGAAATTCTCAA
AAAGATATTGGAAATAAGTATATTAGTATTTTATCAACAGCTGCACAATCTGATGATAAAGATAAATTTACTAAGTTAAC
ACAAGAAAAAAATAAAGAACTTAAAGAAGTAGAAAAAGAAAATACTAAAACTGAGGCTGAATTAAGAAAAGAAATTGCAT
TATGGTCTTACAATGACTACAAAAATATAAAAAAAGCTATAGAATCCAAAAAAGAGATAAAATACTATATTAAAAACAGC
TTAACCAAAGAAGTTTATACTAATTTAGCACCTAAAACTAATATAGATAGTTATATAAAAAATAATTCTATTTATTCTAT
AAGTTTTCCATTAAAATCTGGTAAAACTAAAAATTTTTTAGAAACCAATAATTTACTTAATTCTCTTAACTGGGAAGGAA
ATATTATAATTACAAAGGATTTTAGTTCTAATGGTTATATATCTAAAAATTATAATTATTATAATTCCATTAGAGATAGG
CTTATAAAAGAAATAATCGTAGGTATTTCTTCTTTAATTATGGGAATTGTTATATTAATTATTTTCAAAAGAAGGAATTA
TTTAAATTCTCCTATGTTAAATAAAGTAAAAGGAATATATAATAACATTCCGCTAGATTTGAAGTTATTTATATTTATTT
TATACACTGCTATAATGGGGAGTTATTTAATAAATCTTAGTTTTTTTTATAAACCCTTAGGAATTAATCACTTTATTGTT
ACTACTCTAATAAGCATTTATGCTTTATATTTCATATTCAATTTCAAATATGCTATAAAGTTAAAAAAAGATAAAAATGA
GTTTTTAATTCAATATAAGGGCAGCCTAATTAACAAATTATTAAATGTTATTAAAAACAGTAATTTTAATAAAAATATTA
AATTAAAATTAGCTTCTATTATAACATTTTCTGCTCTATTAGGTGCATTTATACTATTATTATTGCTACTAGGATTTCAC
AATATATTAAGTGCCTTTATTATAACCATACTTTATATAATTTTACTTATAATATACTTATTTAAAAAAGCTGATTATTT
AAATGAAATATTAAAAGCTACAGAAAAAATGTCCTGTGGGAATTTAGATTATGTTATAAAGGAAAAAGGAGAAAGTGTTC
TAGGCAAAATAGCCCATAATATCAATAATATGAAAGAAGGTTATAAAAAATCTCTAGAAGAGCAGGTTAAAAGTGAAAGA
TTAAAAACTGAACTTATAACCAATGTATCTCATGATTTAAAGACTCCCCTTACCTCTATAATAAATTATATAGATTTACT
TAAAAAAGAGGATTTAAGCAAAGATGAAATTAATGGATATATTTCTGTTCTAGATAGAAAGTCTAAAAGACTTAAAGCAC
TTATAGAGGATTTATTTGAGGCATCAAAAATGTCCAGCGGTGCAGTAGAATTAAATATTGAAAAAATAAACGTAACAGCT
TTATTAAAACAAGCTTTAGGAGAATTTGAAGAAAAAATTAAGAAGTCCTCTCTACAGCTTAAATTTAAATGTACTAATAA
TAAAGCTTATGCAAATTTAGATGGAAAGAAAACCTGGAGAGTATTTGAAAATTTAATAAACAATATTATAAAGTATTCTC
AACCTAATACTAGAGTATATATAGATTTAATAGAAACAAATACTAAAATAATAATTACTATGAAAAATGTCTCACGCTAT
GAAATGGATTTTAGTGCTGATGAAATTTTTGAAAGATTTAAAAGGGGAGATAAGGCTAGAAACACAGATGGATCTGGGCT
AGGTCTTGCCATTGCAAAAAGTATTTCAGAACTTCAGGGTGGGAGCTTAAACATTATCATTGATGGGGATCTTTTTAAGG
TTATTGTAGAGTTTAACAAATAA

Upstream 100 bases:

>100_bases
AATCCTAGAGAACCTAGGTACTTGAAGGTGGTGTGGGGAATTGGATATAAAATTCAAGAATAAATTTATTTATATAATAT
GTTTCATTGTAGGTATATAT

Downstream 100 bases:

>100_bases
TATATTTAACTTTGGTTTCACTAATATAAAAAATAAGATTATTTATATATTGTTCTAATATTTTATAACTATTCTCAAAA
ATATATAATTTATAACTTTT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 700; Mature: 700

Protein sequence:

>700_residues
MLSLSILTGYDLIKNRKYLVKSPYFNSYEFDMEIYSYCSNLHNFHIAYKNFDYKVAENKVTKEQLADLKLFYEDMIRNSQ
KDIGNKYISILSTAAQSDDKDKFTKLTQEKNKELKEVEKENTKTEAELRKEIALWSYNDYKNIKKAIESKKEIKYYIKNS
LTKEVYTNLAPKTNIDSYIKNNSIYSISFPLKSGKTKNFLETNNLLNSLNWEGNIIITKDFSSNGYISKNYNYYNSIRDR
LIKEIIVGISSLIMGIVILIIFKRRNYLNSPMLNKVKGIYNNIPLDLKLFIFILYTAIMGSYLINLSFFYKPLGINHFIV
TTLISIYALYFIFNFKYAIKLKKDKNEFLIQYKGSLINKLLNVIKNSNFNKNIKLKLASIITFSALLGAFILLLLLLGFH
NILSAFIITILYIILLIIYLFKKADYLNEILKATEKMSCGNLDYVIKEKGESVLGKIAHNINNMKEGYKKSLEEQVKSER
LKTELITNVSHDLKTPLTSIINYIDLLKKEDLSKDEINGYISVLDRKSKRLKALIEDLFEASKMSSGAVELNIEKINVTA
LLKQALGEFEEKIKKSSLQLKFKCTNNKAYANLDGKKTWRVFENLINNIIKYSQPNTRVYIDLIETNTKIIITMKNVSRY
EMDFSADEIFERFKRGDKARNTDGSGLGLAIAKSISELQGGSLNIIIDGDLFKVIVEFNK

Sequences:

>Translated_700_residues
MLSLSILTGYDLIKNRKYLVKSPYFNSYEFDMEIYSYCSNLHNFHIAYKNFDYKVAENKVTKEQLADLKLFYEDMIRNSQ
KDIGNKYISILSTAAQSDDKDKFTKLTQEKNKELKEVEKENTKTEAELRKEIALWSYNDYKNIKKAIESKKEIKYYIKNS
LTKEVYTNLAPKTNIDSYIKNNSIYSISFPLKSGKTKNFLETNNLLNSLNWEGNIIITKDFSSNGYISKNYNYYNSIRDR
LIKEIIVGISSLIMGIVILIIFKRRNYLNSPMLNKVKGIYNNIPLDLKLFIFILYTAIMGSYLINLSFFYKPLGINHFIV
TTLISIYALYFIFNFKYAIKLKKDKNEFLIQYKGSLINKLLNVIKNSNFNKNIKLKLASIITFSALLGAFILLLLLLGFH
NILSAFIITILYIILLIIYLFKKADYLNEILKATEKMSCGNLDYVIKEKGESVLGKIAHNINNMKEGYKKSLEEQVKSER
LKTELITNVSHDLKTPLTSIINYIDLLKKEDLSKDEINGYISVLDRKSKRLKALIEDLFEASKMSSGAVELNIEKINVTA
LLKQALGEFEEKIKKSSLQLKFKCTNNKAYANLDGKKTWRVFENLINNIIKYSQPNTRVYIDLIETNTKIIITMKNVSRY
EMDFSADEIFERFKRGDKARNTDGSGLGLAIAKSISELQGGSLNIIIDGDLFKVIVEFNK
>Mature_700_residues
MLSLSILTGYDLIKNRKYLVKSPYFNSYEFDMEIYSYCSNLHNFHIAYKNFDYKVAENKVTKEQLADLKLFYEDMIRNSQ
KDIGNKYISILSTAAQSDDKDKFTKLTQEKNKELKEVEKENTKTEAELRKEIALWSYNDYKNIKKAIESKKEIKYYIKNS
LTKEVYTNLAPKTNIDSYIKNNSIYSISFPLKSGKTKNFLETNNLLNSLNWEGNIIITKDFSSNGYISKNYNYYNSIRDR
LIKEIIVGISSLIMGIVILIIFKRRNYLNSPMLNKVKGIYNNIPLDLKLFIFILYTAIMGSYLINLSFFYKPLGINHFIV
TTLISIYALYFIFNFKYAIKLKKDKNEFLIQYKGSLINKLLNVIKNSNFNKNIKLKLASIITFSALLGAFILLLLLLGFH
NILSAFIITILYIILLIIYLFKKADYLNEILKATEKMSCGNLDYVIKEKGESVLGKIAHNINNMKEGYKKSLEEQVKSER
LKTELITNVSHDLKTPLTSIINYIDLLKKEDLSKDEINGYISVLDRKSKRLKALIEDLFEASKMSSGAVELNIEKINVTA
LLKQALGEFEEKIKKSSLQLKFKCTNNKAYANLDGKKTWRVFENLINNIIKYSQPNTRVYIDLIETNTKIIITMKNVSRY
EMDFSADEIFERFKRGDKARNTDGSGLGLAIAKSISELQGGSLNIIIDGDLFKVIVEFNK

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=257, Percent_Identity=24.5136186770428, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787894, Length=206, Percent_Identity=27.6699029126214, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI1788713, Length=211, Percent_Identity=24.6445497630332, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1789149, Length=263, Percent_Identity=24.7148288973384, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786600, Length=210, Percent_Identity=25.2380952380952, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786912, Length=236, Percent_Identity=25, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1788279, Length=209, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI87082128, Length=231, Percent_Identity=30.7359307359307, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786783, Length=291, Percent_Identity=18.213058419244, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790346, Length=276, Percent_Identity=24.6376811594203, Blast_Score=64, Evalue=5e-11,
Organism=Escherichia coli, GI145693157, Length=232, Percent_Identity=23.7068965517241, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 80904; Mature: 80904

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLSILTGYDLIKNRKYLVKSPYFNSYEFDMEIYSYCSNLHNFHIAYKNFDYKVAENKV
CEEEEEECCHHHHHCCCCEEECCCCCCEEEHHHHHHHHHCCCCEEEEEECCCEEECCCHH
TKEQLADLKLFYEDMIRNSQKDIGNKYISILSTAAQSDDKDKFTKLTQEKNKELKEVEKE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NTKTEAELRKEIALWSYNDYKNIKKAIESKKEIKYYIKNSLTKEVYTNLAPKTNIDSYIK
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHC
NNSIYSISFPLKSGKTKNFLETNNLLNSLNWEGNIIITKDFSSNGYISKNYNYYNSIRDR
CCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEECCCCHHHHHHHH
LIKEIIVGISSLIMGIVILIIFKRRNYLNSPMLNKVKGIYNNIPLDLKLFIFILYTAIMG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SYLINLSFFYKPLGINHFIVTTLISIYALYFIFNFKYAIKLKKDKNEFLIQYKGSLINKL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCEEEEECHHHHHHH
LNVIKNSNFNKNIKLKLASIITFSALLGAFILLLLLLGFHNILSAFIITILYIILLIIYL
HHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKKADYLNEILKATEKMSCGNLDYVIKEKGESVLGKIAHNINNMKEGYKKSLEEQVKSER
HHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKTELITNVSHDLKTPLTSIINYIDLLKKEDLSKDEINGYISVLDRKSKRLKALIEDLFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASKMSSGAVELNIEKINVTALLKQALGEFEEKIKKSSLQLKFKCTNNKAYANLDGKKTWR
HHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCCCHHHHH
VFENLINNIIKYSQPNTRVYIDLIETNTKIIITMKNVSRYEMDFSADEIFERFKRGDKAR
HHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEEECCCHHCCCCCHHHHHHHHHCCCCCC
NTDGSGLGLAIAKSISELQGGSLNIIIDGDLFKVIVEFNK
CCCCCCHHHHHHHHHHHHCCCCEEEEECCHHEEHHEECCC
>Mature Secondary Structure
MLSLSILTGYDLIKNRKYLVKSPYFNSYEFDMEIYSYCSNLHNFHIAYKNFDYKVAENKV
CEEEEEECCHHHHHCCCCEEECCCCCCEEEHHHHHHHHHCCCCEEEEEECCCEEECCCHH
TKEQLADLKLFYEDMIRNSQKDIGNKYISILSTAAQSDDKDKFTKLTQEKNKELKEVEKE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NTKTEAELRKEIALWSYNDYKNIKKAIESKKEIKYYIKNSLTKEVYTNLAPKTNIDSYIK
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHC
NNSIYSISFPLKSGKTKNFLETNNLLNSLNWEGNIIITKDFSSNGYISKNYNYYNSIRDR
CCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEECCCCHHHHHHHH
LIKEIIVGISSLIMGIVILIIFKRRNYLNSPMLNKVKGIYNNIPLDLKLFIFILYTAIMG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SYLINLSFFYKPLGINHFIVTTLISIYALYFIFNFKYAIKLKKDKNEFLIQYKGSLINKL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCEEEEECHHHHHHH
LNVIKNSNFNKNIKLKLASIITFSALLGAFILLLLLLGFHNILSAFIITILYIILLIIYL
HHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKKADYLNEILKATEKMSCGNLDYVIKEKGESVLGKIAHNINNMKEGYKKSLEEQVKSER
HHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKTELITNVSHDLKTPLTSIINYIDLLKKEDLSKDEINGYISVLDRKSKRLKALIEDLFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASKMSSGAVELNIEKINVTALLKQALGEFEEKIKKSSLQLKFKCTNNKAYANLDGKKTWR
HHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCCCHHHHH
VFENLINNIIKYSQPNTRVYIDLIETNTKIIITMKNVSRYEMDFSADEIFERFKRGDKAR
HHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEEECCCHHCCCCCHHHHHHHHHCCCCCC
NTDGSGLGLAIAKSISELQGGSLNIIIDGDLFKVIVEFNK
CCCCCCHHHHHHHHHHHHCCCCEEEEECCHHEEHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]