Click here to switch to the map view.

The map label for this gene is pcrA

Identifier: 170755491

GI number: 170755491

Start: 3537634

End: 3539850

Strand: Reverse

Name: pcrA

Synonym: CLD_1251

Alternate gene names: 170755491

Gene position: 3539850-3537634 (Counterclockwise)

Preceding gene: 170754650

Following gene: 170756080

Centisome position: 89.43

GC content: 28.1

Gene sequence:

>2217_bases
ATGGATTTAAAAAATAAATTAAATAAAGAACAATATGAAGCTGCTATTACTATAGATGGACCTTTACTAATACTCGCAGG
AGCAGGTTCAGGCAAGACAAGGGTTTTGACTTATAGAATAGCTCACATGATAGAAAATTTAAATATATATCCTTCTAAAA
TTTTGGCCATAACTTTTACAAATAAGGCTGCTGGAGAAATGAAAGAAAGAATAAAGGCTTTAGTAGGGGATGTGGTAGAA
GGAATGTGGGTTTCTACATTTCACTCTAGCTGTGTAAGGATTTTAAGAAGAGAAATAGATAAGTTAGGTTATGATAAAAA
TTTTACTATATATGATACTTATGATCAAAAAACTTTAGTTAAGCAATGTATGGAAGAATTGAATATAAATGATAAAGAAA
TCACAGACAGAGAAGTAATAAATACCATAAGTAATCAAAAGGATAATTTAATATCACCTAAGGAATTTAAAAAAATTTCT
AATGGAAATTATAGAAAAGATAGAATTGCAGATGCATATATTTTATACCAAAAAAAATTAAAGACTAATAATGCATTAGA
TTTTGATGATTTGATATATAAAACTGTGGAGTTATTTAAAACAAATAAAGAGGTATTAGAATTTTACCAAAGAAAATTTA
GATACATAATGGTGGATGAATATCAAGATACTAACAAATGCCAATATGAATTAGTTAAATTATTAGCTTCAGTTCATAGA
AATATATGTGTAGTTGGTGATGATGATCAATGCATATATGAATGGAGAGGGGCAGATATAAGCAATATATTAAATTTTGA
GAAGGATTATAAAGAAGCTAAGGTTATAAAATTAGAGCAAAATTATAGGTCAAAGGGCAATATATTAAACGCTGCCAATG
AGGTTATAAAAAATAATTCTCAAAGAAAAAATAAAGTTTTAAGAACTGAAAATGAAAATGGAAACAAGATAAAAGTATTT
AGAGCATATTCTGATATAGATGAAGCAAAATTTGCAGCCTCTGAAATCAAAAATATAATAAAGGATAGTAAAAAATCTTT
TAATGATTTTGCAGTTTTATATAGAACTAACGCACAATCCCGTATTTTTGAAGATGTGTTTATGAAAAGAGATATCCCTT
ATAGATTAATAGGTGGATTAAAGTTTTATGATAGAAAAGAAATAAAGGATTTAATGGCTTATTTAAAGCTTATAAACAAT
CCATTAGATGATATAAGTTTAAGGAGAATAATAAATGTTCCTAAAAGGAGTATAGGTGATGCTACAGTAAAAAAAGTTCA
AGAATTTGCAAATGAAATGGATGAATGTATGTATAGCGTGCTTTTAGATGCGGATCAAATATTAACATTGTCTCAAAGAA
GTATAACATCTATCAAAAAGTTCGTAAGTATAATGAATAGCTTTATAAGAAAAAAAGATGAGATAAGTGTATCTGCTCTT
ATAAAAGAAATATTAGAAGAAACAGGATACTTAAAAGAATTAAAAAATTCTAAGAATCCAGATGATGTAAGTAGAGTAGA
AAATCTGAAAGAATTAGTTTCCGCGGCAGCGGATTTTGAAAGAGAATCAGAAGATAAATCTTTAGGAGCGTTTTTAGAAA
AAGTAGCATTGGTTACAGATATAGATAATTATGATGAAAATTCAGATAGTGTTGCTATGATGACAGTACATAGTGCTAAG
GGATTAGAATTTCCTGTAGTATTTATGGTAGGTATGGAAAATGGAATATTTCCAGGAACACAATCATTGTCAGATCCAAA
AGAAATGGAAGAGTCTAGAAGACTGTGTTATGTTGCTATAACAAGAGCAAAAGAACAGCTATATATAACTTCAGCAGAAA
TTAGAAAAGTATTTGGGAGAACCGTAGCTTATGCTTTATCAGATTTTGTAAGTGAGATATCCAAAGATTTAAAAGATAAC
GTAAATATTACGGGAGAAGTAGCAACTGATAAAGTATTGCATAAAAATTTTGGTATAAGATCACAAAAAACAAATAGTAT
TAATTCTTATAAATCACCTAGCTTAAATAATGTACCTAAAAATAATGTGGATTCTAATAAGCTTAATGTAGGTTTAAAGG
TTAAGCATAAAGATTTTGGAATAGGAACTATAGTTAGTATGAGTAAGGTTTCGGATGACATAAAGTTAACTATTGCTTTT
GACCGTAGAGGTGTTAAAATATTAATGCTTAGTATGGCGCCAATTGAAGCGGTTTAG

Upstream 100 bases:

>100_bases
TTTACTAGCACATTTTAAATTTATAAGAATGTTTAACTAATATTTAAAATGATATAATATTAAGAAGAGGTAAAACAGAT
AAACTTAGGGAGCTGATGGA

Downstream 100 bases:

>100_bases
GAGGGATATATATGGATAACAAGTTAGAAAAGATGAAGGAACTTGTAGAAGAATTAAATCAGTATGCCTATGAGTATTAT
GTATTAGATAATCCCAGTAT

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 738; Mature: 738

Protein sequence:

>738_residues
MDLKNKLNKEQYEAAITIDGPLLILAGAGSGKTRVLTYRIAHMIENLNIYPSKILAITFTNKAAGEMKERIKALVGDVVE
GMWVSTFHSSCVRILRREIDKLGYDKNFTIYDTYDQKTLVKQCMEELNINDKEITDREVINTISNQKDNLISPKEFKKIS
NGNYRKDRIADAYILYQKKLKTNNALDFDDLIYKTVELFKTNKEVLEFYQRKFRYIMVDEYQDTNKCQYELVKLLASVHR
NICVVGDDDQCIYEWRGADISNILNFEKDYKEAKVIKLEQNYRSKGNILNAANEVIKNNSQRKNKVLRTENENGNKIKVF
RAYSDIDEAKFAASEIKNIIKDSKKSFNDFAVLYRTNAQSRIFEDVFMKRDIPYRLIGGLKFYDRKEIKDLMAYLKLINN
PLDDISLRRIINVPKRSIGDATVKKVQEFANEMDECMYSVLLDADQILTLSQRSITSIKKFVSIMNSFIRKKDEISVSAL
IKEILEETGYLKELKNSKNPDDVSRVENLKELVSAAADFERESEDKSLGAFLEKVALVTDIDNYDENSDSVAMMTVHSAK
GLEFPVVFMVGMENGIFPGTQSLSDPKEMEESRRLCYVAITRAKEQLYITSAEIRKVFGRTVAYALSDFVSEISKDLKDN
VNITGEVATDKVLHKNFGIRSQKTNSINSYKSPSLNNVPKNNVDSNKLNVGLKVKHKDFGIGTIVSMSKVSDDIKLTIAF
DRRGVKILMLSMAPIEAV

Sequences:

>Translated_738_residues
MDLKNKLNKEQYEAAITIDGPLLILAGAGSGKTRVLTYRIAHMIENLNIYPSKILAITFTNKAAGEMKERIKALVGDVVE
GMWVSTFHSSCVRILRREIDKLGYDKNFTIYDTYDQKTLVKQCMEELNINDKEITDREVINTISNQKDNLISPKEFKKIS
NGNYRKDRIADAYILYQKKLKTNNALDFDDLIYKTVELFKTNKEVLEFYQRKFRYIMVDEYQDTNKCQYELVKLLASVHR
NICVVGDDDQCIYEWRGADISNILNFEKDYKEAKVIKLEQNYRSKGNILNAANEVIKNNSQRKNKVLRTENENGNKIKVF
RAYSDIDEAKFAASEIKNIIKDSKKSFNDFAVLYRTNAQSRIFEDVFMKRDIPYRLIGGLKFYDRKEIKDLMAYLKLINN
PLDDISLRRIINVPKRSIGDATVKKVQEFANEMDECMYSVLLDADQILTLSQRSITSIKKFVSIMNSFIRKKDEISVSAL
IKEILEETGYLKELKNSKNPDDVSRVENLKELVSAAADFERESEDKSLGAFLEKVALVTDIDNYDENSDSVAMMTVHSAK
GLEFPVVFMVGMENGIFPGTQSLSDPKEMEESRRLCYVAITRAKEQLYITSAEIRKVFGRTVAYALSDFVSEISKDLKDN
VNITGEVATDKVLHKNFGIRSQKTNSINSYKSPSLNNVPKNNVDSNKLNVGLKVKHKDFGIGTIVSMSKVSDDIKLTIAF
DRRGVKILMLSMAPIEAV
>Mature_738_residues
MDLKNKLNKEQYEAAITIDGPLLILAGAGSGKTRVLTYRIAHMIENLNIYPSKILAITFTNKAAGEMKERIKALVGDVVE
GMWVSTFHSSCVRILRREIDKLGYDKNFTIYDTYDQKTLVKQCMEELNINDKEITDREVINTISNQKDNLISPKEFKKIS
NGNYRKDRIADAYILYQKKLKTNNALDFDDLIYKTVELFKTNKEVLEFYQRKFRYIMVDEYQDTNKCQYELVKLLASVHR
NICVVGDDDQCIYEWRGADISNILNFEKDYKEAKVIKLEQNYRSKGNILNAANEVIKNNSQRKNKVLRTENENGNKIKVF
RAYSDIDEAKFAASEIKNIIKDSKKSFNDFAVLYRTNAQSRIFEDVFMKRDIPYRLIGGLKFYDRKEIKDLMAYLKLINN
PLDDISLRRIINVPKRSIGDATVKKVQEFANEMDECMYSVLLDADQILTLSQRSITSIKKFVSIMNSFIRKKDEISVSAL
IKEILEETGYLKELKNSKNPDDVSRVENLKELVSAAADFERESEDKSLGAFLEKVALVTDIDNYDENSDSVAMMTVHSAK
GLEFPVVFMVGMENGIFPGTQSLSDPKEMEESRRLCYVAITRAKEQLYITSAEIRKVFGRTVAYALSDFVSEISKDLKDN
VNITGEVATDKVLHKNFGIRSQKTNSINSYKSPSLNNVPKNNVDSNKLNVGLKVKHKDFGIGTIVSMSKVSDDIKLTIAF
DRRGVKILMLSMAPIEAV

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=739, Percent_Identity=36.9418132611637, Blast_Score=520, Evalue=1e-148,
Organism=Escherichia coli, GI48994965, Length=640, Percent_Identity=41.09375, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1787196, Length=369, Percent_Identity=26.8292682926829, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=746, Percent_Identity=28.28418230563, Blast_Score=221, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6324477, Length=747, Percent_Identity=23.6947791164659, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 84423; Mature: 84423

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKNKLNKEQYEAAITIDGPLLILAGAGSGKTRVLTYRIAHMIENLNIYPSKILAITFT
CCCHHHCCHHHCCEEEEECCCEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCEEEEEEEC
NKAAGEMKERIKALVGDVVEGMWVSTFHSSCVRILRREIDKLGYDKNFTIYDTYDQKTLV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHH
KQCMEELNINDKEITDREVINTISNQKDNLISPKEFKKISNGNYRKDRIADAYILYQKKL
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KTNNALDFDDLIYKTVELFKTNKEVLEFYQRKFRYIMVDEYQDTNKCQYELVKLLASVHR
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHCC
NICVVGDDDQCIYEWRGADISNILNFEKDYKEAKVIKLEQNYRSKGNILNAANEVIKNNS
CEEEECCCCCEEEEECCCCHHHHHHHHHHHCHHEEEEEHHCCCCCCCHHHHHHHHHHCCC
QRKNKVLRTENENGNKIKVFRAYSDIDEAKFAASEIKNIIKDSKKSFNDFAVLYRTNAQS
HHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHH
RIFEDVFMKRDIPYRLIGGLKFYDRKEIKDLMAYLKLINNPLDDISLRRIINVPKRSIGD
HHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCH
ATVKKVQEFANEMDECMYSVLLDADQILTLSQRSITSIKKFVSIMNSFIRKKDEISVSAL
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
IKEILEETGYLKELKNSKNPDDVSRVENLKELVSAAADFERESEDKSLGAFLEKVALVTD
HHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
IDNYDENSDSVAMMTVHSAKGLEFPVVFMVGMENGIFPGTQSLSDPKEMEESRRLCYVAI
CCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCEEEEEE
TRAKEQLYITSAEIRKVFGRTVAYALSDFVSEISKDLKDNVNITGEVATDKVLHKNFGIR
ECCHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCC
SQKTNSINSYKSPSLNNVPKNNVDSNKLNVGLKVKHKDFGIGTIVSMSKVSDDIKLTIAF
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEE
DRRGVKILMLSMAPIEAV
CCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MDLKNKLNKEQYEAAITIDGPLLILAGAGSGKTRVLTYRIAHMIENLNIYPSKILAITFT
CCCHHHCCHHHCCEEEEECCCEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCEEEEEEEC
NKAAGEMKERIKALVGDVVEGMWVSTFHSSCVRILRREIDKLGYDKNFTIYDTYDQKTLV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHH
KQCMEELNINDKEITDREVINTISNQKDNLISPKEFKKISNGNYRKDRIADAYILYQKKL
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KTNNALDFDDLIYKTVELFKTNKEVLEFYQRKFRYIMVDEYQDTNKCQYELVKLLASVHR
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHCC
NICVVGDDDQCIYEWRGADISNILNFEKDYKEAKVIKLEQNYRSKGNILNAANEVIKNNS
CEEEECCCCCEEEEECCCCHHHHHHHHHHHCHHEEEEEHHCCCCCCCHHHHHHHHHHCCC
QRKNKVLRTENENGNKIKVFRAYSDIDEAKFAASEIKNIIKDSKKSFNDFAVLYRTNAQS
HHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHH
RIFEDVFMKRDIPYRLIGGLKFYDRKEIKDLMAYLKLINNPLDDISLRRIINVPKRSIGD
HHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCH
ATVKKVQEFANEMDECMYSVLLDADQILTLSQRSITSIKKFVSIMNSFIRKKDEISVSAL
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
IKEILEETGYLKELKNSKNPDDVSRVENLKELVSAAADFERESEDKSLGAFLEKVALVTD
HHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
IDNYDENSDSVAMMTVHSAKGLEFPVVFMVGMENGIFPGTQSLSDPKEMEESRRLCYVAI
CCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHCCEEEEEE
TRAKEQLYITSAEIRKVFGRTVAYALSDFVSEISKDLKDNVNITGEVATDKVLHKNFGIR
ECCHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCC
SQKTNSINSYKSPSLNNVPKNNVDSNKLNVGLKVKHKDFGIGTIVSMSKVSDDIKLTIAF
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEE
DRRGVKILMLSMAPIEAV
CCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]