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The map label for this gene is proS

Identifier: 170755400

GI number: 170755400

Start: 3635508

End: 3636944

Strand: Reverse

Name: proS

Synonym: CLD_1176

Alternate gene names: 170755400

Gene position: 3636944-3635508 (Counterclockwise)

Preceding gene: 170754862

Following gene: 170755947

Centisome position: 91.88

GC content: 33.61

Gene sequence:

>1437_bases
ATGGCAAAAGATAAAAAATTTGTTGAAGATATTACACCAATGGATGAAGATTTTGCACAATGGTATACAGACATTGTTAA
AAAGGCAGAGCTTGCTGATTACTCAAGTATCAGAGGATGTATGATTATTCGTCCTAATGGTTATGCGATTTGGGAAAATA
TACAGAAATATGTAGATACAAAACTTAAAGAGTATGGACATGAAAATGTATCTATGCCAATATTTATACCTGAAAACTTA
TTACAAAAAGAAAAAGATCACGTTGAAGGATTTGCACCTGAAGTTGCATGGGTAACTCATGGTGGAGATGACGAATTAGC
TGAAAGATTATGTGTTCGTCCAACATCAGAAACATTGTTCTGTGAACACTATGCTAAGATTGTTCAATCTTATAAAGATT
TACCAAAGCTTTATAACCAATGGTGTTCAGTTGTAAGATGGGAAAAGACAACTAGACCTTTTTTAAGAACAACTGAATTC
TTATGGCAAGAAGGTCATACAATTCATGAAACAAAGGAAGAAGCAGAATCTCATTCACTTAAGATACTAAACATGTATTC
TAGATTATGTGAAGATATGTTAGCAATGCCAGTGGTTATGGGTAAAAAGACAGATAAGGAAAAATTTGCTGGTGCAGATG
ATACGTACACAATTGAAAGTTTAATGCATGATGGAAAGGCACTGCAAGCAGGTACTTCACACTATCTTGGACAAAATTTC
TCAAAGGCATTTGCAATTCAGTTCTCAGATAGAAATGGAAAGTTAGATTATCCACACTATACTACTTGGGCAGTAACTAC
AAGATTAATTGGAGCAATAATAATGGTCCATGGTGATAATAGTGGTCTAAAATTACCACCAAGAATTGCACCAACTCAAG
CAGTGATTATTCCTGTTGCTCAACATAAAGAAGGAGTATTGGAAAAGGCAGAAGAATTAAAGGAAAGATTAGCTAAAGTT
GTAAGAGTTAAGCTTGATGATAGTGATAAGATGCCAGGTTGGAAGTATAGTGAATATGAAATGAAGGGTATCCCACTAAG
AATAGAAATTGGGCCAAAGGATATAGAAAAAAACCAAGCTGTGTTAGTAAGAAGAGACAATAGAGAAAAAACAATAGTTT
CATTGGACGAAATTGAAATAAAGGTTCAAGAGATGTTAGATATTATCCATAATTCAATGCTAGAAGAAGCTAAAAAAACT
AGAGATGAAAAAACTTATGTAGCAACAAATATGGAAGAATTTGAAGATACTATTGAAAATAAACCAGGTTTTATTAAAGC
AATGTGGTGTGGAGATAAAGCTTGTGAAGATAAGATAAGAGAGGTAACTGGCGCTACATCTCGTTGTATGCCATTTGAAC
AAGAGGTTGTTTCAGACACTTGTGTATGTTGTGGTAAAAAAGCTAAAAATTTAGTTTACTGGGGAAGAGCATATTAA

Upstream 100 bases:

>100_bases
ATGTTAACAAGGGTGGTACCACCGACACAACTTCGGTCCCTGTATAGGGATTAGGAGTTTTTTTATTTACAAAAAAATAA
ATTTTAGGGAGGAATTTAAT

Downstream 100 bases:

>100_bases
TTAGAAGTTATTTAATTAATAGATTTTATAAAATAAATATTAGAAAATTAAAGGGTATAGGGGAATCAATGATTTTTCTA
TACCTTTTATATAGTGGGTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MAKDKKFVEDITPMDEDFAQWYTDIVKKAELADYSSIRGCMIIRPNGYAIWENIQKYVDTKLKEYGHENVSMPIFIPENL
LQKEKDHVEGFAPEVAWVTHGGDDELAERLCVRPTSETLFCEHYAKIVQSYKDLPKLYNQWCSVVRWEKTTRPFLRTTEF
LWQEGHTIHETKEEAESHSLKILNMYSRLCEDMLAMPVVMGKKTDKEKFAGADDTYTIESLMHDGKALQAGTSHYLGQNF
SKAFAIQFSDRNGKLDYPHYTTWAVTTRLIGAIIMVHGDNSGLKLPPRIAPTQAVIIPVAQHKEGVLEKAEELKERLAKV
VRVKLDDSDKMPGWKYSEYEMKGIPLRIEIGPKDIEKNQAVLVRRDNREKTIVSLDEIEIKVQEMLDIIHNSMLEEAKKT
RDEKTYVATNMEEFEDTIENKPGFIKAMWCGDKACEDKIREVTGATSRCMPFEQEVVSDTCVCCGKKAKNLVYWGRAY

Sequences:

>Translated_478_residues
MAKDKKFVEDITPMDEDFAQWYTDIVKKAELADYSSIRGCMIIRPNGYAIWENIQKYVDTKLKEYGHENVSMPIFIPENL
LQKEKDHVEGFAPEVAWVTHGGDDELAERLCVRPTSETLFCEHYAKIVQSYKDLPKLYNQWCSVVRWEKTTRPFLRTTEF
LWQEGHTIHETKEEAESHSLKILNMYSRLCEDMLAMPVVMGKKTDKEKFAGADDTYTIESLMHDGKALQAGTSHYLGQNF
SKAFAIQFSDRNGKLDYPHYTTWAVTTRLIGAIIMVHGDNSGLKLPPRIAPTQAVIIPVAQHKEGVLEKAEELKERLAKV
VRVKLDDSDKMPGWKYSEYEMKGIPLRIEIGPKDIEKNQAVLVRRDNREKTIVSLDEIEIKVQEMLDIIHNSMLEEAKKT
RDEKTYVATNMEEFEDTIENKPGFIKAMWCGDKACEDKIREVTGATSRCMPFEQEVVSDTCVCCGKKAKNLVYWGRAY
>Mature_477_residues
AKDKKFVEDITPMDEDFAQWYTDIVKKAELADYSSIRGCMIIRPNGYAIWENIQKYVDTKLKEYGHENVSMPIFIPENLL
QKEKDHVEGFAPEVAWVTHGGDDELAERLCVRPTSETLFCEHYAKIVQSYKDLPKLYNQWCSVVRWEKTTRPFLRTTEFL
WQEGHTIHETKEEAESHSLKILNMYSRLCEDMLAMPVVMGKKTDKEKFAGADDTYTIESLMHDGKALQAGTSHYLGQNFS
KAFAIQFSDRNGKLDYPHYTTWAVTTRLIGAIIMVHGDNSGLKLPPRIAPTQAVIIPVAQHKEGVLEKAEELKERLAKVV
RVKLDDSDKMPGWKYSEYEMKGIPLRIEIGPKDIEKNQAVLVRRDNREKTIVSLDEIEIKVQEMLDIIHNSMLEEAKKTR
DEKTYVATNMEEFEDTIENKPGFIKAMWCGDKACEDKIREVTGATSRCMPFEQEVVSDTCVCCGKKAKNLVYWGRAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=493, Percent_Identity=44.6247464503043, Blast_Score=424, Evalue=1e-119,
Organism=Escherichia coli, GI1786392, Length=156, Percent_Identity=28.8461538461538, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71991342, Length=490, Percent_Identity=39.7959183673469, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71991346, Length=358, Percent_Identity=43.854748603352, Blast_Score=345, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6321808, Length=497, Percent_Identity=45.8752515090543, Blast_Score=446, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24649468, Length=494, Percent_Identity=42.914979757085, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24649466, Length=494, Percent_Identity=42.914979757085, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 55008; Mature: 54876

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDKKFVEDITPMDEDFAQWYTDIVKKAELADYSSIRGCMIIRPNGYAIWENIQKYVDT
CCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHCCCEEEECCCCCHHHHHHHHHHHH
KLKEYGHENVSMPIFIPENLLQKEKDHVEGFAPEVAWVTHGGDDELAERLCVRPTSETLF
HHHHCCCCCCCEEEEECHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCCCHHH
CEHYAKIVQSYKDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQEGHTIHETKEEAESHSL
HHHHHHHHHHHHHHHHHHHHHHHHEECCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KILNMYSRLCEDMLAMPVVMGKKTDKEKFAGADDTYTIESLMHDGKALQAGTSHYLGQNF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHCCCCHHHCCCHHHHCCCC
SKAFAIQFSDRNGKLDYPHYTTWAVTTRLIGAIIMVHGDNSGLKLPPRIAPTQAVIIPVA
CCEEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCEEEEECH
QHKEGVLEKAEELKERLAKVVRVKLDDSDKMPGWKYSEYEMKGIPLRIEIGPKDIEKNQA
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCE
VLVRRDNREKTIVSLDEIEIKVQEMLDIIHNSMLEEAKKTRDEKTYVATNMEEFEDTIEN
EEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHCC
KPGFIKAMWCGDKACEDKIREVTGATSRCMPFEQEVVSDTCVCCGKKAKNLVYWGRAY
CCCEEEEEECCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCCCEEEECCCC
>Mature Secondary Structure 
AKDKKFVEDITPMDEDFAQWYTDIVKKAELADYSSIRGCMIIRPNGYAIWENIQKYVDT
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHCCCEEEECCCCCHHHHHHHHHHHH
KLKEYGHENVSMPIFIPENLLQKEKDHVEGFAPEVAWVTHGGDDELAERLCVRPTSETLF
HHHHCCCCCCCEEEEECHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCCCHHH
CEHYAKIVQSYKDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQEGHTIHETKEEAESHSL
HHHHHHHHHHHHHHHHHHHHHHHHEECCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KILNMYSRLCEDMLAMPVVMGKKTDKEKFAGADDTYTIESLMHDGKALQAGTSHYLGQNF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHCCCCHHHCCCHHHHCCCC
SKAFAIQFSDRNGKLDYPHYTTWAVTTRLIGAIIMVHGDNSGLKLPPRIAPTQAVIIPVA
CCEEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCEEEEECH
QHKEGVLEKAEELKERLAKVVRVKLDDSDKMPGWKYSEYEMKGIPLRIEIGPKDIEKNQA
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCE
VLVRRDNREKTIVSLDEIEIKVQEMLDIIHNSMLEEAKKTRDEKTYVATNMEEFEDTIEN
EEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHCC
KPGFIKAMWCGDKACEDKIREVTGATSRCMPFEQEVVSDTCVCCGKKAKNLVYWGRAY
CCCEEEEEECCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA