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The map label for this gene is rumA

Identifier: 170754817

GI number: 170754817

Start: 3672735

End: 3674120

Strand: Reverse

Name: rumA

Synonym: CLD_1140

Alternate gene names: 170754817

Gene position: 3674120-3672735 (Counterclockwise)

Preceding gene: 170757353

Following gene: 170755609

Centisome position: 92.82

GC content: 28.72

Gene sequence:

>1386_bases
ATGAAAAAAAATATACCTGTAGAAAAAAATAAAGAATACAATTTAGACATAATAGGCACTGGATTTGAAGGAGAAGGGGT
AAGTAAAATTGATGATTTTACAGTATTTATTCCAGGAGCCATAAAAGAAGAAAATGCATTAACAAGAATAGTTAAAGTAA
ATAAAAACTTCGCCTTTGGAAAGATTATAAAGTTAAACAAAACATCAGAAAATAGAGTAGAATCTGTATGTCCTATATAT
AAACAGTGTGGAGGATGTCAAGTTCAACACTATAGTTATAAGGCTCAATTAGAGTTTAAAAAGCAGAGGGTTATAGATTC
ACTAGAAAGAATAGGTAAACTAGACATAGATAAAATAAAAATAGAAGATACTATTGGCATGGAAAACCCGTATAGATATA
GAAACAAAATTCAAATGCCTGTAAGATTTGAAAAAGGTGAAATAAAAATAGGATTTTATAAATCAAGAACTCATGAAGTA
GCAGATGTAAAAGAATGTTTTATACAAGATGAGCAAGCAGATAAAATTATACAAATAATAAGAAATTGGATGGTAAAACA
TAATATATCTGCCTACAATGAAGAAACAGGAAAAGGGTTTATAAGGCATATAATGGTAAGGAAAGCTTTTAAAAATAAAG
AACTTATGTTAGTTATTGTAACTAATAAAGAAGAGGAAATACCTTACAAAGAAGAATTAATAAATGAAGTAATAAAAGAA
TTTTTAAATATAAAAAGTATAATACAAAATATAAATACTAAAAAAACCAATGTGATTTTAGGAGAAAAGTGTATTACATT
ATGGGGAAAAGATACCATAACTGACTATATAGATAAGTTTAAATTTAATATATCTCCATTATCTTTTTTTCAGGTAAATC
CCATACAAACAGAGGTATTGTATAAAAAAGCCTTAGAATTTGCAAATCTAAAAGGAGAAGAAATAGTATTTGATGCTTAC
TGTGGCACAGGAACTATATCCTTATTTTTATCACAGAAAGCTAAAAAAGTATACGGAGTAGAAATAATAAAGGAAGCTAT
AGACAACGCAAAAGATAATGCAAAAGAAAATAGGATAGATAATGCAGAATTTTTTGTAGGAAAATCAGAAGAAGTTATAC
CAGATTTAATAGATAAAGGTATAAAAGCAGATGTGGTAGTTGTAGATCCACCAAGAAAAGGCTGCGAAAAAACCTTACTA
GAAGCTATAGCAGCTACAAAACCTAAAAGAATAGTATATGTATCCTGTGATCCAGGAACTTTGGCTAGAGACTTAGGTAT
ATTAAATGGATTAGGTTATGAAACTAAAGAAGTACAAACAGTAGATATGTTCCCCCAAACTAGCCATGTGGAGACTATTG
TGTTGATACAACGAGCCGATACGTAG

Upstream 100 bases:

>100_bases
AATTTATATATTGGAAAGAACCTAAGGATAGATAGTCTAATAGTTACTATTTATCCTGTGTTTTTGTTATGATATAAAAT
TACATAAATGGAGAGAGAGT

Downstream 100 bases:

>100_bases
AAATCCTTTGTTTTAGGCACTTCTTCAACCATTTTGTTTTTATAAATGAGGGTGATAAAATCCGTAATAAACAGCTAAAA
AAATATATTAACGTTTCTGT

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKKNIPVEKNKEYNLDIIGTGFEGEGVSKIDDFTVFIPGAIKEENALTRIVKVNKNFAFGKIIKLNKTSENRVESVCPIY
KQCGGCQVQHYSYKAQLEFKKQRVIDSLERIGKLDIDKIKIEDTIGMENPYRYRNKIQMPVRFEKGEIKIGFYKSRTHEV
ADVKECFIQDEQADKIIQIIRNWMVKHNISAYNEETGKGFIRHIMVRKAFKNKELMLVIVTNKEEEIPYKEELINEVIKE
FLNIKSIIQNINTKKTNVILGEKCITLWGKDTITDYIDKFKFNISPLSFFQVNPIQTEVLYKKALEFANLKGEEIVFDAY
CGTGTISLFLSQKAKKVYGVEIIKEAIDNAKDNAKENRIDNAEFFVGKSEEVIPDLIDKGIKADVVVVDPPRKGCEKTLL
EAIAATKPKRIVYVSCDPGTLARDLGILNGLGYETKEVQTVDMFPQTSHVETIVLIQRADT

Sequences:

>Translated_461_residues
MKKNIPVEKNKEYNLDIIGTGFEGEGVSKIDDFTVFIPGAIKEENALTRIVKVNKNFAFGKIIKLNKTSENRVESVCPIY
KQCGGCQVQHYSYKAQLEFKKQRVIDSLERIGKLDIDKIKIEDTIGMENPYRYRNKIQMPVRFEKGEIKIGFYKSRTHEV
ADVKECFIQDEQADKIIQIIRNWMVKHNISAYNEETGKGFIRHIMVRKAFKNKELMLVIVTNKEEEIPYKEELINEVIKE
FLNIKSIIQNINTKKTNVILGEKCITLWGKDTITDYIDKFKFNISPLSFFQVNPIQTEVLYKKALEFANLKGEEIVFDAY
CGTGTISLFLSQKAKKVYGVEIIKEAIDNAKDNAKENRIDNAEFFVGKSEEVIPDLIDKGIKADVVVVDPPRKGCEKTLL
EAIAATKPKRIVYVSCDPGTLARDLGILNGLGYETKEVQTVDMFPQTSHVETIVLIQRADT
>Mature_461_residues
MKKNIPVEKNKEYNLDIIGTGFEGEGVSKIDDFTVFIPGAIKEENALTRIVKVNKNFAFGKIIKLNKTSENRVESVCPIY
KQCGGCQVQHYSYKAQLEFKKQRVIDSLERIGKLDIDKIKIEDTIGMENPYRYRNKIQMPVRFEKGEIKIGFYKSRTHEV
ADVKECFIQDEQADKIIQIIRNWMVKHNISAYNEETGKGFIRHIMVRKAFKNKELMLVIVTNKEEEIPYKEELINEVIKE
FLNIKSIIQNINTKKTNVILGEKCITLWGKDTITDYIDKFKFNISPLSFFQVNPIQTEVLYKKALEFANLKGEEIVFDAY
CGTGTISLFLSQKAKKVYGVEIIKEAIDNAKDNAKENRIDNAEFFVGKSEEVIPDLIDKGIKADVVVVDPPRKGCEKTLL
EAIAATKPKRIVYVSCDPGTLARDLGILNGLGYETKEVQTVDMFPQTSHVETIVLIQRADT

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=352, Percent_Identity=28.6931818181818, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI51173878, Length=352, Percent_Identity=28.6931818181818, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI269784680, Length=287, Percent_Identity=28.2229965156794, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI269784676, Length=287, Percent_Identity=28.2229965156794, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI20149688, Length=287, Percent_Identity=28.2229965156794, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI269784678, Length=288, Percent_Identity=28.4722222222222, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1789148, Length=439, Percent_Identity=28.0182232346241, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1787083, Length=325, Percent_Identity=25.2307692307692, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790403, Length=182, Percent_Identity=30.2197802197802, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71997438, Length=166, Percent_Identity=35.5421686746988, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6322909, Length=375, Percent_Identity=27.2, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24666579, Length=436, Percent_Identity=29.5871559633028, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 52689; Mature: 52689

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNIPVEKNKEYNLDIIGTGFEGEGVSKIDDFTVFIPGAIKEENALTRIVKVNKNFAFG
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHEEEEEECCCCCEE
KIIKLNKTSENRVESVCPIYKQCGGCQVQHYSYKAQLEFKKQRVIDSLERIGKLDIDKIK
EEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCEEE
IEDTIGMENPYRYRNKIQMPVRFEKGEIKIGFYKSRTHEVADVKECFIQDEQADKIIQII
EECCCCCCCCHHHHCEECCCEEEECCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHH
RNWMVKHNISAYNEETGKGFIRHIMVRKAFKNKELMLVIVTNKEEEIPYKEELINEVIKE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
FLNIKSIIQNINTKKTNVILGEKCITLWGKDTITDYIDKFKFNISPLSFFQVNPIQTEVL
HHHHHHHHHCCCCCCCEEEECCCEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHH
YKKALEFANLKGEEIVFDAYCGTGTISLFLSQKAKKVYGVEIIKEAIDNAKDNAKENRID
HHHHHHHHCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
NAEFFVGKSEEVIPDLIDKGIKADVVVVDPPRKGCEKTLLEAIAATKPKRIVYVSCDPGT
CCEEEECCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCH
LARDLGILNGLGYETKEVQTVDMFPQTSHVETIVLIQRADT
HHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MKKNIPVEKNKEYNLDIIGTGFEGEGVSKIDDFTVFIPGAIKEENALTRIVKVNKNFAFG
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHEEEEEECCCCCEE
KIIKLNKTSENRVESVCPIYKQCGGCQVQHYSYKAQLEFKKQRVIDSLERIGKLDIDKIK
EEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCEEE
IEDTIGMENPYRYRNKIQMPVRFEKGEIKIGFYKSRTHEVADVKECFIQDEQADKIIQII
EECCCCCCCCHHHHCEECCCEEEECCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHH
RNWMVKHNISAYNEETGKGFIRHIMVRKAFKNKELMLVIVTNKEEEIPYKEELINEVIKE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
FLNIKSIIQNINTKKTNVILGEKCITLWGKDTITDYIDKFKFNISPLSFFQVNPIQTEVL
HHHHHHHHHCCCCCCCEEEECCCEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHH
YKKALEFANLKGEEIVFDAYCGTGTISLFLSQKAKKVYGVEIIKEAIDNAKDNAKENRID
HHHHHHHHCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
NAEFFVGKSEEVIPDLIDKGIKADVVVVDPPRKGCEKTLLEAIAATKPKRIVYVSCDPGT
CCEEEECCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCH
LARDLGILNGLGYETKEVQTVDMFPQTSHVETIVLIQRADT
HHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]