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The map label for this gene is mdeA [H]

Identifier: 170752124

GI number: 170752124

Start: 6064113

End: 6065393

Strand: Direct

Name: mdeA [H]

Synonym: Mrad2831_5757

Alternate gene names: 170752124

Gene position: 6064113-6065393 (Clockwise)

Preceding gene: 170752123

Following gene: 170752126

Centisome position: 99.77

GC content: 68.31

Gene sequence:

>1281_bases
ATGTCCGACCGCCTGCCCGGCTTCAACACGCTGGCGATCCACGCGGGTGCCGCCCCCGACGCGGCCACCGGCGCACGGGC
CACGCCGATCTACCAGACCACCAGCTTCGTGTTCGACGACGTCGACCACGCCGCGTCGCTGTTCGGTCTTCAGGCCTTCG
GCAACATCTACACGCGGATCACCAACCCGACGAACGCCGTGCTGGAGGAGCGCATCGCGGCGTTGGAGGGCGGGACCGCC
GCCCTGGCGGTCGCCTCCGGCCACGCGGCCGAGTTTCTGACCATGCACGCCCTGATGCAGCCGGGCGACGAGTTCGTGGC
CTCGAACAAGCTGTACGGCGGCTCGATCAACCAGTTCAACCACTCGTACAAGAACTTCGGCTGGTCGGTGGCCTGGGCCG
ACAACGACGACCCCGCCTCCTTCGAGGCGGCGATCACGCCGCGCACCAAGGCGATCTTCTGCGAATCGATCGCCAATCCC
GGCGGCGTCATCACCGACCTCGCGGCGCTCTCGCAGATCGCCAAGAGGCACAACATCCCGCTCGTCGTCGACAACACCAT
GGCGACGCCGTACCTGATCCGACCCTTCGAGCACGGCGCCGACATCGTGGTGCACTCGGCCACCAAGTTCCTCGGCGGCC
ACGGCAACTCGATCGGCGGCCTGATCGTCGACGGCGGCACGTTCCAGTGGGCCGGTGACGCGCGCTACCCGATGATGAGC
CAGCCGCGGCCGGAATATTCCGGCATGGTGCTGGCCGAGACCTTCGGCAATTTCGGCTTCGCCATCGCCTGCCGTGTGCT
GGGCCTGCGCGATCTCGGTCCGGCGCTGTCGCCGTTCAACGCCTTCCTGATCCTGAACGGCATCGAGACGCTGCCGCTGC
GCATGCAGCGGCACTCCGACAACGCGCTGACGGTAGCCAAGCACCTGTCGACGCATCCGGCGGTGTCGTGGGTGAGCTAT
CCCGGCCTGGAGACCGACCGCTACCATCAGCTCGCGCGGCGCTACACGCCGAACGGCGCCGGCGCCGTCTTCACCTTCGG
GCTGAAGGGCGGCTACGACGCGGGGGTCAAGCTCGTCTCGAGTCTCCAGCTGTTCTCGCACCTCGCCAATATCGGCGACA
CCCGCTCGCTGATCATCCACCCGGCCTCGACGACCCACCGCCAGCTCACCGACGCGCAGAAGACCGCCGCGGGCGCGGGA
CCCGACGTGGTGCGGCTGTCGATCGGCCTGGAGGATCCGGCCGACCTGATCGACGATCTCGACGCCGCTCTCGGGGGCTG
A

Upstream 100 bases:

>100_bases
GCCGGACGCCTAGGGATTGCGGCGCGCGCGGCCGCGGGCGAGTCTTGCGCCGCAAACCCGGGCGGGCGTACAGGCTCGGG
TGCTCTGAGGAGACCCGACG

Downstream 100 bases:

>100_bases
CGAGCCGACGCCGGATGGCCCGGCCGCGCCGAGACCGGCGATCGCCGCGAGGCGACCCCGGCGGCGCGTCCGGGCCCGAC
GTCGCCCGCCGCCCGGGTCT

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSDRLPGFNTLAIHAGAAPDAATGARATPIYQTTSFVFDDVDHAASLFGLQAFGNIYTRITNPTNAVLEERIAALEGGTA
ALAVASGHAAEFLTMHALMQPGDEFVASNKLYGGSINQFNHSYKNFGWSVAWADNDDPASFEAAITPRTKAIFCESIANP
GGVITDLAALSQIAKRHNIPLVVDNTMATPYLIRPFEHGADIVVHSATKFLGGHGNSIGGLIVDGGTFQWAGDARYPMMS
QPRPEYSGMVLAETFGNFGFAIACRVLGLRDLGPALSPFNAFLILNGIETLPLRMQRHSDNALTVAKHLSTHPAVSWVSY
PGLETDRYHQLARRYTPNGAGAVFTFGLKGGYDAGVKLVSSLQLFSHLANIGDTRSLIIHPASTTHRQLTDAQKTAAGAG
PDVVRLSIGLEDPADLIDDLDAALGG

Sequences:

>Translated_426_residues
MSDRLPGFNTLAIHAGAAPDAATGARATPIYQTTSFVFDDVDHAASLFGLQAFGNIYTRITNPTNAVLEERIAALEGGTA
ALAVASGHAAEFLTMHALMQPGDEFVASNKLYGGSINQFNHSYKNFGWSVAWADNDDPASFEAAITPRTKAIFCESIANP
GGVITDLAALSQIAKRHNIPLVVDNTMATPYLIRPFEHGADIVVHSATKFLGGHGNSIGGLIVDGGTFQWAGDARYPMMS
QPRPEYSGMVLAETFGNFGFAIACRVLGLRDLGPALSPFNAFLILNGIETLPLRMQRHSDNALTVAKHLSTHPAVSWVSY
PGLETDRYHQLARRYTPNGAGAVFTFGLKGGYDAGVKLVSSLQLFSHLANIGDTRSLIIHPASTTHRQLTDAQKTAAGAG
PDVVRLSIGLEDPADLIDDLDAALGG
>Mature_425_residues
SDRLPGFNTLAIHAGAAPDAATGARATPIYQTTSFVFDDVDHAASLFGLQAFGNIYTRITNPTNAVLEERIAALEGGTAA
LAVASGHAAEFLTMHALMQPGDEFVASNKLYGGSINQFNHSYKNFGWSVAWADNDDPASFEAAITPRTKAIFCESIANPG
GVITDLAALSQIAKRHNIPLVVDNTMATPYLIRPFEHGADIVVHSATKFLGGHGNSIGGLIVDGGTFQWAGDARYPMMSQ
PRPEYSGMVLAETFGNFGFAIACRVLGLRDLGPALSPFNAFLILNGIETLPLRMQRHSDNALTVAKHLSTHPAVSWVSYP
GLETDRYHQLARRYTPNGAGAVFTFGLKGGYDAGVKLVSSLQLFSHLANIGDTRSLIIHPASTTHRQLTDAQKTAAGAGP
DVVRLSIGLEDPADLIDDLDAALGG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=405, Percent_Identity=32.5925925925926, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI299473759, Length=406, Percent_Identity=30.0492610837438, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI25453487, Length=405, Percent_Identity=29.1358024691358, Blast_Score=142, Evalue=5e-34,
Organism=Escherichia coli, GI1790375, Length=412, Percent_Identity=29.126213592233, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1789383, Length=436, Percent_Identity=27.0642201834862, Blast_Score=124, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71980708, Length=416, Percent_Identity=31.7307692307692, Blast_Score=197, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=412, Percent_Identity=32.5242718446602, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=412, Percent_Identity=31.5533980582524, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=46.4037122969838, Blast_Score=354, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6319307, Length=423, Percent_Identity=30.7328605200946, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6321254, Length=442, Percent_Identity=25.5656108597285, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321170, Length=363, Percent_Identity=26.7217630853994, Blast_Score=106, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6321904, Length=429, Percent_Identity=24.7086247086247, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922554, Length=423, Percent_Identity=30.9692671394799, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45228; Mature: 45097

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRLPGFNTLAIHAGAAPDAATGARATPIYQTTSFVFDDVDHAASLFGLQAFGNIYTRI
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCEEE
TNPTNAVLEERIAALEGGTAALAVASGHAAEFLTMHALMQPGDEFVASNKLYGGSINQFN
CCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHC
HSYKNFGWSVAWADNDDPASFEAAITPRTKAIFCESIANPGGVITDLAALSQIAKRHNIP
CHHHCCCEEEEECCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
LVVDNTMATPYLIRPFEHGADIVVHSATKFLGGHGNSIGGLIVDGGTFQWAGDARYPMMS
EEEECCCCCCEEECCCCCCCCEEEECHHHHHCCCCCCCCEEEEECCEEEECCCCCCCCCC
QPRPEYSGMVLAETFGNFGFAIACRVLGLRDLGPALSPFNAFLILNGIETLPLRMQRHSD
CCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEECCCC
NALTVAKHLSTHPAVSWVSYPGLETDRYHQLARRYTPNGAGAVFTFGLKGGYDAGVKLVS
CHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHH
SLQLFSHLANIGDTRSLIIHPASTTHRQLTDAQKTAAGAGPDVVRLSIGLEDPADLIDDL
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHH
DAALGG
HHHHCC
>Mature Secondary Structure 
SDRLPGFNTLAIHAGAAPDAATGARATPIYQTTSFVFDDVDHAASLFGLQAFGNIYTRI
CCCCCCCCEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCEEE
TNPTNAVLEERIAALEGGTAALAVASGHAAEFLTMHALMQPGDEFVASNKLYGGSINQFN
CCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHC
HSYKNFGWSVAWADNDDPASFEAAITPRTKAIFCESIANPGGVITDLAALSQIAKRHNIP
CHHHCCCEEEEECCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
LVVDNTMATPYLIRPFEHGADIVVHSATKFLGGHGNSIGGLIVDGGTFQWAGDARYPMMS
EEEECCCCCCEEECCCCCCCCEEEECHHHHHCCCCCCCCEEEEECCEEEECCCCCCCCCC
QPRPEYSGMVLAETFGNFGFAIACRVLGLRDLGPALSPFNAFLILNGIETLPLRMQRHSD
CCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEECCCC
NALTVAKHLSTHPAVSWVSYPGLETDRYHQLARRYTPNGAGAVFTFGLKGGYDAGVKLVS
CHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHH
SLQLFSHLANIGDTRSLIIHPASTTHRQLTDAQKTAAGAGPDVVRLSIGLEDPADLIDDL
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHH
DAALGG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]